Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF41 and GFF42 are separated by 74 nucleotides GFF42 and GFF43 are separated by 13 nucleotides GFF43 and GFF44 are separated by 100 nucleotides GFF44 and GFF45 are separated by 59 nucleotides
GFF41 - hypothetical protein, at 32,483 to 32,701
GFF41
GFF42 - hypothetical protein, at 32,776 to 33,429
GFF42
GFF43 - Transporter, LysE family, at 33,443 to 34,069
GFF43
GFF44 - Acyl dehydratase, at 34,170 to 34,634
GFF44
GFF45 - secreted alkaline phosphatase, at 34,694 to 36,244
GFF45
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 32.502 kb on + strand at 32.524 kb on - strand, within GFF41 at 32.563 kb on + strand, within GFF41 at 32.636 kb on - strand, within GFF41 at 32.641 kb on + strand, within GFF41 at 32.649 kb on - strand, within GFF41 at 32.649 kb on - strand, within GFF41 at 32.679 kb on + strand, within GFF41 at 32.687 kb on - strand at 32.687 kb on - strand at 32.692 kb on - strand at 32.718 kb on + strand at 32.718 kb on + strand at 32.727 kb on + strand at 32.727 kb on + strand at 32.737 kb on + strand at 32.772 kb on - strand at 32.778 kb on + strand at 32.782 kb on - strand at 32.786 kb on - strand at 32.788 kb on + strand at 32.788 kb on + strand at 32.808 kb on + strand at 32.816 kb on - strand at 32.820 kb on + strand at 32.820 kb on + strand at 32.820 kb on + strand at 32.820 kb on + strand at 32.847 kb on - strand, within GFF42 at 32.919 kb on + strand, within GFF42 at 32.919 kb on + strand, within GFF42 at 32.924 kb on + strand, within GFF42 at 32.927 kb on - strand, within GFF42 at 32.946 kb on + strand, within GFF42 at 32.954 kb on - strand, within GFF42 at 32.985 kb on - strand, within GFF42 at 33.026 kb on - strand, within GFF42 at 33.070 kb on - strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.100 kb on + strand, within GFF42 at 33.105 kb on + strand, within GFF42 at 33.105 kb on + strand, within GFF42 at 33.108 kb on - strand, within GFF42 at 33.118 kb on - strand, within GFF42 at 33.118 kb on - strand, within GFF42 at 33.129 kb on - strand, within GFF42 at 33.144 kb on - strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.195 kb on + strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.203 kb on - strand, within GFF42 at 33.241 kb on + strand, within GFF42 at 33.241 kb on + strand, within GFF42 at 33.242 kb on - strand, within GFF42 at 33.249 kb on - strand, within GFF42 at 33.249 kb on - strand, within GFF42 at 33.250 kb on - strand, within GFF42 at 33.271 kb on + strand, within GFF42 at 33.272 kb on - strand, within GFF42 at 33.272 kb on - strand, within GFF42 at 33.277 kb on + strand, within GFF42 at 33.277 kb on - strand, within GFF42 at 33.290 kb on - strand, within GFF42 at 33.290 kb on - strand, within GFF42 at 33.290 kb on - strand, within GFF42 at 33.290 kb on - strand, within GFF42 at 33.294 kb on - strand, within GFF42 at 33.324 kb on + strand, within GFF42 at 33.369 kb on + strand at 33.377 kb on - strand at 33.377 kb on - strand at 33.387 kb on + strand at 33.388 kb on + strand at 33.395 kb on - strand at 33.406 kb on - strand at 33.414 kb on - strand at 33.471 kb on + strand at 33.476 kb on + strand at 33.476 kb on + strand at 33.476 kb on + strand at 33.479 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.484 kb on - strand at 33.486 kb on + strand at 33.500 kb on + strand at 33.500 kb on + strand at 33.508 kb on - strand, within GFF43 at 33.560 kb on + strand, within GFF43 at 33.599 kb on - strand, within GFF43 at 33.625 kb on + strand, within GFF43 at 33.626 kb on + strand, within GFF43 at 33.626 kb on + strand, within GFF43 at 33.626 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.631 kb on + strand, within GFF43 at 33.634 kb on - strand, within GFF43 at 33.634 kb on - strand, within GFF43 at 33.639 kb on - strand, within GFF43 at 33.639 kb on - strand, within GFF43 at 33.639 kb on - strand, within GFF43 at 33.639 kb on - strand, within GFF43 at 33.691 kb on + strand, within GFF43 at 33.691 kb on + strand, within GFF43 at 33.691 kb on + strand, within GFF43 at 33.696 kb on + strand, within GFF43 at 33.699 kb on - strand, within GFF43 at 33.699 kb on - strand, within GFF43 at 33.708 kb on + strand, within GFF43 at 33.718 kb on + strand, within GFF43 at 33.718 kb on + strand, within GFF43 at 33.739 kb on - strand, within GFF43 at 33.769 kb on + strand, within GFF43 at 33.782 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.855 kb on - strand, within GFF43 at 33.867 kb on + strand, within GFF43 at 33.867 kb on + strand, within GFF43 at 33.875 kb on - strand, within GFF43 at 33.930 kb on + strand, within GFF43 at 33.969 kb on - strand, within GFF43 at 33.990 kb on - strand, within GFF43 at 34.013 kb on + strand at 34.015 kb on + strand at 34.030 kb on + strand at 34.030 kb on + strand at 34.034 kb on - strand at 34.059 kb on + strand at 34.059 kb on + strand at 34.059 kb on + strand at 34.067 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.077 kb on - strand at 34.079 kb on - strand at 34.089 kb on - strand at 34.096 kb on - strand at 34.129 kb on + strand at 34.129 kb on + strand at 34.137 kb on - strand at 34.138 kb on + strand at 34.145 kb on - strand at 34.166 kb on - strand at 34.166 kb on - strand at 34.171 kb on - strand at 34.171 kb on - strand at 34.176 kb on - strand at 34.206 kb on + strand at 34.210 kb on - strand at 34.214 kb on + strand at 34.217 kb on - strand, within GFF44 at 34.217 kb on - strand, within GFF44 at 34.217 kb on - strand, within GFF44 at 34.227 kb on - strand, within GFF44 at 34.263 kb on + strand, within GFF44 at 34.271 kb on - strand, within GFF44 at 34.312 kb on + strand, within GFF44 at 34.323 kb on + strand, within GFF44 at 34.364 kb on - strand, within GFF44 at 34.366 kb on - strand, within GFF44 at 34.366 kb on - strand, within GFF44 at 34.377 kb on - strand, within GFF44 at 34.384 kb on - strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.455 kb on - strand, within GFF44 at 34.464 kb on + strand, within GFF44 at 34.464 kb on + strand, within GFF44 at 34.511 kb on + strand, within GFF44 at 34.511 kb on + strand, within GFF44 at 34.519 kb on - strand, within GFF44 at 34.525 kb on + strand, within GFF44 at 34.546 kb on - strand, within GFF44 at 34.670 kb on - strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.716 kb on - strand at 34.716 kb on - strand at 34.730 kb on + strand at 34.738 kb on - strand at 34.739 kb on - strand at 34.775 kb on - strand at 34.800 kb on + strand at 34.800 kb on + strand at 34.800 kb on + strand at 34.808 kb on - strand at 34.826 kb on + strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.846 kb on - strand at 34.879 kb on + strand, within GFF45 at 34.879 kb on - strand, within GFF45 at 34.903 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.017 kb on - strand, within GFF45 at 35.034 kb on + strand, within GFF45 at 35.042 kb on - strand, within GFF45 at 35.060 kb on + strand, within GFF45 at 35.060 kb on + strand, within GFF45 at 35.068 kb on + strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 32,502 + +0.3 32,524 - GFF41 0.19 +1.3 32,563 + GFF41 0.37 -0.3 32,636 - GFF41 0.70 +1.4 32,641 + GFF41 0.72 +1.1 32,649 - GFF41 0.76 +1.1 32,649 - GFF41 0.76 +1.2 32,679 + GFF41 0.89 +0.2 32,687 - +0.2 32,687 - +0.7 32,692 - +0.9 32,718 + +1.2 32,718 + +0.4 32,727 + -1.4 32,727 + +2.0 32,737 + +0.6 32,772 - -0.4 32,778 + -0.2 32,782 - -0.5 32,786 - +0.7 32,788 + -1.0 32,788 + -2.0 32,808 + +0.2 32,816 - -0.5 32,820 + -1.3 32,820 + +0.6 32,820 + +0.4 32,820 + -0.4 32,847 - GFF42 0.11 +0.2 32,919 + GFF42 0.22 -1.2 32,919 + GFF42 0.22 +0.7 32,924 + GFF42 0.23 +0.3 32,927 - GFF42 0.23 -0.7 32,946 + GFF42 0.26 -0.2 32,954 - GFF42 0.27 +0.4 32,985 - GFF42 0.32 +0.1 33,026 - GFF42 0.38 -0.3 33,070 - GFF42 0.45 -0.7 33,100 + GFF42 0.50 +0.5 33,100 + GFF42 0.50 -0.9 33,100 + GFF42 0.50 -0.3 33,100 + GFF42 0.50 +0.8 33,100 + GFF42 0.50 -1.8 33,100 + GFF42 0.50 +1.5 33,100 + GFF42 0.50 +0.0 33,105 + GFF42 0.50 +0.8 33,105 + GFF42 0.50 -0.6 33,108 - GFF42 0.51 +0.3 33,118 - GFF42 0.52 +0.1 33,118 - GFF42 0.52 -0.0 33,129 - GFF42 0.54 +0.3 33,144 - GFF42 0.56 -0.2 33,195 + GFF42 0.64 -1.0 33,195 + GFF42 0.64 +1.5 33,195 + GFF42 0.64 +2.2 33,195 + GFF42 0.64 +0.4 33,195 + GFF42 0.64 +0.4 33,195 + GFF42 0.64 +0.9 33,195 + GFF42 0.64 +2.0 33,195 + GFF42 0.64 -0.9 33,195 + GFF42 0.64 -0.0 33,195 + GFF42 0.64 +0.9 33,195 + GFF42 0.64 +0.0 33,195 + GFF42 0.64 -0.3 33,195 + GFF42 0.64 +0.5 33,203 - GFF42 0.65 -0.6 33,203 - GFF42 0.65 -0.2 33,203 - GFF42 0.65 -1.2 33,203 - GFF42 0.65 +0.8 33,203 - GFF42 0.65 -0.5 33,203 - GFF42 0.65 -0.4 33,203 - GFF42 0.65 +0.5 33,203 - GFF42 0.65 -1.8 33,203 - GFF42 0.65 +0.2 33,203 - GFF42 0.65 -0.8 33,203 - GFF42 0.65 +0.3 33,203 - GFF42 0.65 -0.8 33,203 - GFF42 0.65 +0.2 33,241 + GFF42 0.71 +1.0 33,241 + GFF42 0.71 -0.2 33,242 - GFF42 0.71 +0.6 33,249 - GFF42 0.72 +0.8 33,249 - GFF42 0.72 +0.4 33,250 - GFF42 0.72 +1.0 33,271 + GFF42 0.76 +0.1 33,272 - GFF42 0.76 -0.2 33,272 - GFF42 0.76 -0.6 33,277 + GFF42 0.77 -0.7 33,277 - GFF42 0.77 +0.4 33,290 - GFF42 0.79 +0.5 33,290 - GFF42 0.79 -0.8 33,290 - GFF42 0.79 +0.2 33,290 - GFF42 0.79 -0.1 33,294 - GFF42 0.79 -0.4 33,324 + GFF42 0.84 +0.2 33,369 + -0.3 33,377 - -0.3 33,377 - -0.4 33,387 + -0.2 33,388 + -1.5 33,395 - -2.7 33,406 - +0.0 33,414 - +0.1 33,471 + -0.3 33,476 + +1.3 33,476 + -1.4 33,476 + +0.6 33,479 - +0.8 33,484 - +0.2 33,484 - -0.1 33,484 - -0.7 33,484 - +0.3 33,484 - +0.4 33,484 - +0.2 33,484 - +0.2 33,484 - +0.5 33,484 - -0.4 33,484 - -0.3 33,486 + -0.8 33,500 + +0.9 33,500 + -1.1 33,508 - GFF43 0.10 -1.3 33,560 + GFF43 0.19 -0.4 33,599 - GFF43 0.25 +0.5 33,625 + GFF43 0.29 -2.5 33,626 + GFF43 0.29 +0.1 33,626 + GFF43 0.29 -1.4 33,626 + GFF43 0.29 -0.2 33,631 + GFF43 0.30 +0.4 33,631 + GFF43 0.30 +0.6 33,631 + GFF43 0.30 -0.2 33,631 + GFF43 0.30 +0.3 33,631 + GFF43 0.30 -0.3 33,631 + GFF43 0.30 +0.7 33,631 + GFF43 0.30 -1.8 33,634 - GFF43 0.30 +0.6 33,634 - GFF43 0.30 +0.6 33,639 - GFF43 0.31 -0.2 33,639 - GFF43 0.31 +1.3 33,639 - GFF43 0.31 -0.1 33,639 - GFF43 0.31 +0.7 33,691 + GFF43 0.40 +1.1 33,691 + GFF43 0.40 +0.2 33,691 + GFF43 0.40 -0.2 33,696 + GFF43 0.40 +0.1 33,699 - GFF43 0.41 +1.5 33,699 - GFF43 0.41 +1.2 33,708 + GFF43 0.42 -1.9 33,718 + GFF43 0.44 -0.6 33,718 + GFF43 0.44 -1.0 33,739 - GFF43 0.47 -0.9 33,769 + GFF43 0.52 +1.1 33,782 - GFF43 0.54 +1.1 33,852 - GFF43 0.65 +0.6 33,852 - GFF43 0.65 +0.8 33,852 - GFF43 0.65 +0.2 33,855 - GFF43 0.66 +0.8 33,867 + GFF43 0.68 -1.5 33,867 + GFF43 0.68 -0.6 33,875 - GFF43 0.69 +1.6 33,930 + GFF43 0.78 -1.8 33,969 - GFF43 0.84 -0.2 33,990 - GFF43 0.87 +1.3 34,013 + +0.8 34,015 + +0.7 34,030 + -1.7 34,030 + +0.2 34,034 - -1.0 34,059 + -0.2 34,059 + -0.8 34,059 + -0.2 34,067 - -0.1 34,072 - +0.4 34,072 - +0.6 34,072 - +0.5 34,072 - -0.1 34,072 - +1.0 34,072 - -0.6 34,072 - -1.3 34,077 - +0.6 34,079 - +0.6 34,089 - +2.0 34,096 - +1.2 34,129 + +1.4 34,129 + +0.0 34,137 - +1.2 34,138 + +3.2 34,145 - +1.4 34,166 - -0.4 34,166 - -0.5 34,171 - -0.1 34,171 - -0.3 34,176 - -0.1 34,206 + -1.0 34,210 - -0.2 34,214 + +0.3 34,217 - GFF44 0.10 +1.0 34,217 - GFF44 0.10 +1.2 34,217 - GFF44 0.10 -0.7 34,227 - GFF44 0.12 +1.3 34,263 + GFF44 0.20 -0.7 34,271 - GFF44 0.22 -0.2 34,312 + GFF44 0.31 +0.4 34,323 + GFF44 0.33 -0.2 34,364 - GFF44 0.42 -0.6 34,366 - GFF44 0.42 +1.5 34,366 - GFF44 0.42 +0.2 34,377 - GFF44 0.45 +1.3 34,384 - GFF44 0.46 +0.1 34,442 + GFF44 0.58 -0.3 34,442 + GFF44 0.58 -0.0 34,442 + GFF44 0.58 +0.2 34,442 + GFF44 0.58 -0.3 34,442 + GFF44 0.58 +1.3 34,450 - GFF44 0.60 +0.3 34,450 - GFF44 0.60 +0.8 34,450 - GFF44 0.60 +0.4 34,455 - GFF44 0.61 +1.0 34,464 + GFF44 0.63 -0.9 34,464 + GFF44 0.63 +0.8 34,511 + GFF44 0.73 -1.3 34,511 + GFF44 0.73 +0.4 34,519 - GFF44 0.75 -0.0 34,525 + GFF44 0.76 +0.2 34,546 - GFF44 0.81 +1.5 34,670 - +0.2 34,708 + -0.6 34,708 + +1.6 34,708 + -2.6 34,708 + -0.6 34,716 - -1.0 34,716 - +0.7 34,730 + -0.2 34,738 - -0.6 34,739 - -0.5 34,775 - -0.9 34,800 + -1.5 34,800 + -0.2 34,800 + +0.8 34,808 - +0.1 34,826 + -0.0 34,834 - -2.4 34,834 - -1.0 34,834 - +0.3 34,834 - -1.0 34,846 - -0.2 34,879 + GFF45 0.12 -1.4 34,879 - GFF45 0.12 +0.1 34,903 + GFF45 0.13 +0.5 35,009 + GFF45 0.20 -1.0 35,009 + GFF45 0.20 -0.3 35,009 + GFF45 0.20 +1.2 35,017 - GFF45 0.21 -0.9 35,034 + GFF45 0.22 +0.2 35,042 - GFF45 0.22 +0.8 35,060 + GFF45 0.24 +2.2 35,060 + GFF45 0.24 -0.4 35,068 + GFF45 0.24 +0.3 35,068 - GFF45 0.24 -0.2 35,068 - GFF45 0.24 -0.2 35,068 - GFF45 0.24 -0.0 35,068 - GFF45 0.24 +0.5
Or see this region's nucleotide sequence