Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF315 and GFF316 are separated by 40 nucleotides GFF316 and GFF317 are separated by 2 nucleotides GFF317 and GFF318 overlap by 1 nucleotides GFF318 and GFF319 overlap by 11 nucleotides
GFF315 - pyridoxal-phosphate dependent enzyme family protein, at 310,882 to 311,874
GFF315
GFF316 - Diaminopimelate epimerase (EC 5.1.1.7), at 311,915 to 312,727
GFF316
GFF317 - tRNA t(6)A37-methylthiotransferase (EC 2.8.4.5), at 312,730 to 313,971
GFF317
GFF318 - Signal recognition particle receptor FtsY, at 313,971 to 314,909
GFF318
GFF319 - Intracellular septation protein IspA, at 314,899 to 315,552
GFF319
Position (kb)
312
313
314 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 311.752 kb on - strand, within GFF315 at 311.766 kb on + strand, within GFF315 at 311.774 kb on + strand, within GFF315 at 311.774 kb on + strand, within GFF315 at 311.774 kb on + strand, within GFF315 at 311.774 kb on + strand, within GFF315 at 311.777 kb on - strand at 311.779 kb on + strand at 311.779 kb on + strand at 311.779 kb on + strand at 311.779 kb on + strand at 311.782 kb on - strand at 311.782 kb on - strand at 311.787 kb on - strand at 311.803 kb on - strand at 311.808 kb on + strand at 311.808 kb on + strand at 311.816 kb on - strand at 311.816 kb on - strand at 311.816 kb on - strand at 311.838 kb on + strand at 311.838 kb on + strand at 311.846 kb on - strand at 311.876 kb on - strand at 311.891 kb on - strand at 311.891 kb on - strand at 311.901 kb on - strand at 311.901 kb on - strand at 311.912 kb on + strand at 311.912 kb on + strand at 312.710 kb on + strand at 312.710 kb on + strand at 312.718 kb on - strand at 312.718 kb on - strand at 312.720 kb on + strand at 312.728 kb on - strand at 312.729 kb on - strand at 312.736 kb on + strand at 312.737 kb on + strand at 312.737 kb on + strand at 312.737 kb on + strand at 312.737 kb on + strand at 312.737 kb on + strand at 312.742 kb on + strand at 312.745 kb on - strand at 312.746 kb on + strand at 312.746 kb on + strand at 312.752 kb on - strand at 312.754 kb on - strand at 312.754 kb on - strand at 312.754 kb on - strand at 312.754 kb on - strand at 312.759 kb on - strand at 312.759 kb on - strand at 312.764 kb on - strand at 312.770 kb on + strand at 312.780 kb on + strand at 312.780 kb on + strand at 312.780 kb on + strand at 312.788 kb on - strand at 312.788 kb on - strand at 312.788 kb on - strand at 312.806 kb on + strand at 312.816 kb on - strand at 312.826 kb on + strand at 312.834 kb on - strand at 312.836 kb on + strand at 312.842 kb on - strand at 312.844 kb on + strand at 312.847 kb on - strand at 312.857 kb on - strand, within GFF317 at 312.857 kb on - strand, within GFF317 at 312.862 kb on + strand, within GFF317 at 312.866 kb on + strand, within GFF317 at 312.869 kb on - strand, within GFF317 at 312.870 kb on - strand, within GFF317 at 312.892 kb on + strand, within GFF317 at 312.932 kb on + strand, within GFF317 at 312.937 kb on + strand, within GFF317 at 312.940 kb on - strand, within GFF317 at 312.942 kb on + strand, within GFF317 at 312.945 kb on - strand, within GFF317 at 312.948 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.949 kb on + strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 312.957 kb on - strand, within GFF317 at 313.004 kb on + strand, within GFF317 at 313.005 kb on + strand, within GFF317 at 313.005 kb on + strand, within GFF317 at 313.013 kb on - strand, within GFF317 at 313.041 kb on + strand, within GFF317 at 313.041 kb on + strand, within GFF317 at 313.042 kb on - strand, within GFF317 at 313.049 kb on - strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.056 kb on + strand, within GFF317 at 313.064 kb on - strand, within GFF317 at 313.064 kb on - strand, within GFF317 at 313.064 kb on - strand, within GFF317 at 313.064 kb on - strand, within GFF317 at 313.068 kb on + strand, within GFF317 at 313.073 kb on + strand, within GFF317 at 313.078 kb on + strand, within GFF317 at 313.078 kb on + strand, within GFF317 at 313.086 kb on - strand, within GFF317 at 313.086 kb on - strand, within GFF317 at 313.090 kb on + strand, within GFF317 at 313.090 kb on + strand, within GFF317 at 313.091 kb on - strand, within GFF317 at 313.098 kb on - strand, within GFF317 at 313.121 kb on + strand, within GFF317 at 313.129 kb on + strand, within GFF317 at 313.129 kb on + strand, within GFF317 at 313.129 kb on + strand, within GFF317 at 313.129 kb on + strand, within GFF317 at 313.134 kb on + strand, within GFF317 at 313.134 kb on + strand, within GFF317 at 313.136 kb on - strand, within GFF317 at 313.137 kb on - strand, within GFF317 at 313.147 kb on - strand, within GFF317 at 313.149 kb on + strand, within GFF317 at 313.157 kb on - strand, within GFF317 at 313.223 kb on + strand, within GFF317 at 313.223 kb on + strand, within GFF317 at 313.244 kb on - strand, within GFF317 at 313.260 kb on - strand, within GFF317 at 313.269 kb on - strand, within GFF317 at 313.269 kb on - strand, within GFF317 at 313.269 kb on - strand, within GFF317 at 313.271 kb on + strand, within GFF317 at 313.271 kb on + strand, within GFF317 at 313.271 kb on + strand, within GFF317 at 313.279 kb on - strand, within GFF317 at 313.287 kb on + strand, within GFF317 at 313.291 kb on + strand, within GFF317 at 313.292 kb on + strand, within GFF317 at 313.292 kb on + strand, within GFF317 at 313.292 kb on + strand, within GFF317 at 313.292 kb on + strand, within GFF317 at 313.300 kb on + strand, within GFF317 at 313.300 kb on - strand, within GFF317 at 313.305 kb on + strand, within GFF317 at 313.308 kb on - strand, within GFF317 at 313.308 kb on - strand, within GFF317 at 313.310 kb on + strand, within GFF317 at 313.313 kb on - strand, within GFF317 at 313.351 kb on + strand, within GFF317 at 313.351 kb on + strand, within GFF317 at 313.391 kb on + strand, within GFF317 at 313.396 kb on - strand, within GFF317 at 313.427 kb on + strand, within GFF317 at 313.427 kb on + strand, within GFF317 at 313.427 kb on + strand, within GFF317 at 313.427 kb on + strand, within GFF317 at 313.427 kb on + strand, within GFF317 at 313.430 kb on - strand, within GFF317 at 313.434 kb on + strand, within GFF317 at 313.435 kb on - strand, within GFF317 at 313.435 kb on - strand, within GFF317 at 313.451 kb on + strand, within GFF317 at 313.466 kb on - strand, within GFF317 at 313.473 kb on - strand, within GFF317 at 313.486 kb on + strand, within GFF317 at 313.486 kb on + strand, within GFF317 at 313.486 kb on + strand, within GFF317 at 313.494 kb on - strand, within GFF317 at 313.521 kb on - strand, within GFF317 at 313.522 kb on + strand, within GFF317 at 313.525 kb on + strand, within GFF317 at 313.525 kb on + strand, within GFF317 at 313.526 kb on - strand, within GFF317 at 313.538 kb on - strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.545 kb on + strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.553 kb on - strand, within GFF317 at 313.564 kb on + strand, within GFF317 at 313.577 kb on + strand, within GFF317 at 313.631 kb on + strand, within GFF317 at 313.631 kb on + strand, within GFF317 at 313.631 kb on + strand, within GFF317 at 313.631 kb on + strand, within GFF317 at 313.631 kb on + strand, within GFF317 at 313.639 kb on - strand, within GFF317 at 313.639 kb on - strand, within GFF317 at 313.639 kb on - strand, within GFF317 at 313.639 kb on - strand, within GFF317 at 313.639 kb on - strand, within GFF317 at 313.660 kb on + strand, within GFF317 at 313.666 kb on + strand, within GFF317 at 313.672 kb on + strand, within GFF317 at 313.672 kb on + strand, within GFF317 at 313.672 kb on + strand, within GFF317 at 313.672 kb on + strand, within GFF317 at 313.672 kb on + strand, within GFF317 at 313.674 kb on - strand, within GFF317 at 313.679 kb on + strand, within GFF317 at 313.680 kb on - strand, within GFF317 at 313.744 kb on - strand, within GFF317 at 313.751 kb on + strand, within GFF317 at 313.753 kb on + strand, within GFF317 at 313.756 kb on + strand, within GFF317 at 313.758 kb on + strand, within GFF317 at 313.758 kb on + strand, within GFF317 at 313.759 kb on - strand, within GFF317 at 313.766 kb on - strand, within GFF317 at 313.766 kb on - strand, within GFF317 at 313.766 kb on - strand, within GFF317 at 313.785 kb on - strand, within GFF317 at 313.796 kb on - strand, within GFF317 at 313.806 kb on + strand, within GFF317 at 313.806 kb on + strand, within GFF317 at 313.814 kb on - strand, within GFF317 at 313.814 kb on - strand, within GFF317 at 313.820 kb on + strand, within GFF317 at 313.828 kb on - strand, within GFF317 at 313.828 kb on - strand, within GFF317 at 313.833 kb on - strand, within GFF317 at 313.838 kb on + strand, within GFF317 at 313.848 kb on + strand at 313.856 kb on - strand at 313.856 kb on - strand at 313.878 kb on + strand at 313.881 kb on + strand at 313.891 kb on + strand at 313.891 kb on + strand at 313.904 kb on - strand at 313.912 kb on - strand at 313.926 kb on - strand at 313.931 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 311,752 - GFF315 0.88 +1.5 311,766 + GFF315 0.89 +0.3 311,774 + GFF315 0.90 +0.3 311,774 + GFF315 0.90 -1.2 311,774 + GFF315 0.90 +0.2 311,774 + GFF315 0.90 -1.3 311,777 - -0.6 311,779 + -2.0 311,779 + -1.1 311,779 + +1.1 311,779 + -0.4 311,782 - -0.7 311,782 - +0.9 311,787 - +0.0 311,803 - -0.2 311,808 + -0.5 311,808 + -0.2 311,816 - -0.8 311,816 - -0.4 311,816 - +0.0 311,838 + +0.0 311,838 + -2.7 311,846 - -1.4 311,876 - -0.7 311,891 - -0.6 311,891 - -0.3 311,901 - +0.8 311,901 - -0.6 311,912 + +0.5 311,912 + -0.4 312,710 + -1.7 312,710 + -1.9 312,718 - -1.5 312,718 - -1.9 312,720 + +0.4 312,728 - -0.7 312,729 - -0.7 312,736 + -1.2 312,737 + +0.0 312,737 + +0.0 312,737 + -0.4 312,737 + +0.1 312,737 + +0.2 312,742 + -0.2 312,745 - -0.8 312,746 + -0.9 312,746 + -1.1 312,752 - -0.2 312,754 - +0.9 312,754 - +0.2 312,754 - -0.5 312,754 - +0.4 312,759 - -1.6 312,759 - -1.7 312,764 - -0.3 312,770 + -1.4 312,780 + -0.5 312,780 + -0.8 312,780 + -0.8 312,788 - -0.7 312,788 - +1.0 312,788 - +1.1 312,806 + -1.0 312,816 - -0.5 312,826 + -0.4 312,834 - -0.9 312,836 + +1.4 312,842 - +0.7 312,844 + -0.5 312,847 - +0.8 312,857 - GFF317 0.10 -0.6 312,857 - GFF317 0.10 +0.6 312,862 + GFF317 0.11 -0.2 312,866 + GFF317 0.11 -0.3 312,869 - GFF317 0.11 -0.5 312,870 - GFF317 0.11 -0.5 312,892 + GFF317 0.13 +0.0 312,932 + GFF317 0.16 -0.1 312,937 + GFF317 0.17 +0.1 312,940 - GFF317 0.17 +0.4 312,942 + GFF317 0.17 -0.3 312,945 - GFF317 0.17 -1.5 312,948 + GFF317 0.18 +0.5 312,949 + GFF317 0.18 +0.5 312,949 + GFF317 0.18 -0.9 312,949 + GFF317 0.18 -0.9 312,949 + GFF317 0.18 +0.9 312,949 + GFF317 0.18 -0.4 312,949 + GFF317 0.18 -0.7 312,949 + GFF317 0.18 -0.6 312,949 + GFF317 0.18 -1.6 312,949 + GFF317 0.18 -0.9 312,949 + GFF317 0.18 -1.7 312,949 + GFF317 0.18 +1.2 312,949 + GFF317 0.18 -1.1 312,949 + GFF317 0.18 +0.0 312,957 - GFF317 0.18 -2.3 312,957 - GFF317 0.18 +2.0 312,957 - GFF317 0.18 -0.1 312,957 - GFF317 0.18 -1.2 312,957 - GFF317 0.18 +0.7 312,957 - GFF317 0.18 -1.4 312,957 - GFF317 0.18 -0.4 312,957 - GFF317 0.18 -1.7 312,957 - GFF317 0.18 -0.1 313,004 + GFF317 0.22 +0.3 313,005 + GFF317 0.22 +0.8 313,005 + GFF317 0.22 +1.3 313,013 - GFF317 0.23 -0.6 313,041 + GFF317 0.25 +0.6 313,041 + GFF317 0.25 -0.1 313,042 - GFF317 0.25 -1.4 313,049 - GFF317 0.26 +0.4 313,056 + GFF317 0.26 +0.2 313,056 + GFF317 0.26 +1.5 313,056 + GFF317 0.26 +1.0 313,056 + GFF317 0.26 -0.8 313,056 + GFF317 0.26 +0.9 313,056 + GFF317 0.26 -0.3 313,064 - GFF317 0.27 -1.4 313,064 - GFF317 0.27 +1.0 313,064 - GFF317 0.27 -1.3 313,064 - GFF317 0.27 -0.7 313,068 + GFF317 0.27 -0.7 313,073 + GFF317 0.28 -1.6 313,078 + GFF317 0.28 +0.6 313,078 + GFF317 0.28 +0.3 313,086 - GFF317 0.29 +0.4 313,086 - GFF317 0.29 +1.1 313,090 + GFF317 0.29 -1.6 313,090 + GFF317 0.29 +0.2 313,091 - GFF317 0.29 +0.8 313,098 - GFF317 0.30 -0.2 313,121 + GFF317 0.31 +1.1 313,129 + GFF317 0.32 -2.3 313,129 + GFF317 0.32 -0.4 313,129 + GFF317 0.32 -0.2 313,129 + GFF317 0.32 -0.7 313,134 + GFF317 0.33 -0.2 313,134 + GFF317 0.33 -0.6 313,136 - GFF317 0.33 +0.3 313,137 - GFF317 0.33 +0.7 313,147 - GFF317 0.34 -0.5 313,149 + GFF317 0.34 -0.8 313,157 - GFF317 0.34 +0.0 313,223 + GFF317 0.40 -1.7 313,223 + GFF317 0.40 +0.6 313,244 - GFF317 0.41 +0.9 313,260 - GFF317 0.43 -2.4 313,269 - GFF317 0.43 +0.6 313,269 - GFF317 0.43 -1.3 313,269 - GFF317 0.43 +0.1 313,271 + GFF317 0.44 -0.2 313,271 + GFF317 0.44 -0.8 313,271 + GFF317 0.44 -0.1 313,279 - GFF317 0.44 -0.4 313,287 + GFF317 0.45 +0.3 313,291 + GFF317 0.45 -0.2 313,292 + GFF317 0.45 -0.1 313,292 + GFF317 0.45 +0.3 313,292 + GFF317 0.45 -1.4 313,292 + GFF317 0.45 +0.3 313,300 + GFF317 0.46 -1.1 313,300 - GFF317 0.46 +0.1 313,305 + GFF317 0.46 -0.7 313,308 - GFF317 0.47 +1.8 313,308 - GFF317 0.47 -0.3 313,310 + GFF317 0.47 -0.3 313,313 - GFF317 0.47 -1.1 313,351 + GFF317 0.50 -0.3 313,351 + GFF317 0.50 -0.6 313,391 + GFF317 0.53 +0.3 313,396 - GFF317 0.54 -0.8 313,427 + GFF317 0.56 +0.0 313,427 + GFF317 0.56 +0.3 313,427 + GFF317 0.56 -0.2 313,427 + GFF317 0.56 +0.6 313,427 + GFF317 0.56 +0.3 313,430 - GFF317 0.56 -0.2 313,434 + GFF317 0.57 -0.7 313,435 - GFF317 0.57 -0.1 313,435 - GFF317 0.57 -0.8 313,451 + GFF317 0.58 +0.6 313,466 - GFF317 0.59 +0.8 313,473 - GFF317 0.60 -0.2 313,486 + GFF317 0.61 +0.3 313,486 + GFF317 0.61 +1.2 313,486 + GFF317 0.61 -1.6 313,494 - GFF317 0.62 -0.7 313,521 - GFF317 0.64 -1.1 313,522 + GFF317 0.64 -1.0 313,525 + GFF317 0.64 +0.8 313,525 + GFF317 0.64 -1.2 313,526 - GFF317 0.64 -1.5 313,538 - GFF317 0.65 -0.7 313,545 + GFF317 0.66 -0.0 313,545 + GFF317 0.66 -0.1 313,545 + GFF317 0.66 -0.2 313,545 + GFF317 0.66 +1.1 313,545 + GFF317 0.66 +0.5 313,545 + GFF317 0.66 +0.4 313,545 + GFF317 0.66 +0.0 313,545 + GFF317 0.66 -1.0 313,545 + GFF317 0.66 -1.2 313,545 + GFF317 0.66 -0.3 313,545 + GFF317 0.66 -0.7 313,545 + GFF317 0.66 -2.1 313,545 + GFF317 0.66 +1.1 313,545 + GFF317 0.66 -1.3 313,545 + GFF317 0.66 -0.4 313,545 + GFF317 0.66 -1.7 313,545 + GFF317 0.66 -0.3 313,545 + GFF317 0.66 -0.9 313,545 + GFF317 0.66 -1.3 313,553 - GFF317 0.66 -2.0 313,553 - GFF317 0.66 -1.0 313,553 - GFF317 0.66 +0.5 313,553 - GFF317 0.66 +0.7 313,553 - GFF317 0.66 +0.0 313,553 - GFF317 0.66 +0.3 313,553 - GFF317 0.66 +0.1 313,553 - GFF317 0.66 +0.5 313,553 - GFF317 0.66 -0.6 313,553 - GFF317 0.66 +0.4 313,553 - GFF317 0.66 -0.3 313,553 - GFF317 0.66 +1.1 313,553 - GFF317 0.66 -0.1 313,553 - GFF317 0.66 +0.5 313,553 - GFF317 0.66 +0.6 313,553 - GFF317 0.66 +0.8 313,553 - GFF317 0.66 +0.2 313,564 + GFF317 0.67 +0.7 313,577 + GFF317 0.68 +1.4 313,631 + GFF317 0.73 -0.0 313,631 + GFF317 0.73 +0.1 313,631 + GFF317 0.73 -1.6 313,631 + GFF317 0.73 +1.3 313,631 + GFF317 0.73 +0.7 313,639 - GFF317 0.73 +0.1 313,639 - GFF317 0.73 -0.4 313,639 - GFF317 0.73 +0.7 313,639 - GFF317 0.73 -0.4 313,639 - GFF317 0.73 +0.4 313,660 + GFF317 0.75 +0.7 313,666 + GFF317 0.75 -1.2 313,672 + GFF317 0.76 +0.7 313,672 + GFF317 0.76 -0.6 313,672 + GFF317 0.76 -0.1 313,672 + GFF317 0.76 +1.5 313,672 + GFF317 0.76 +0.5 313,674 - GFF317 0.76 -1.3 313,679 + GFF317 0.76 -0.5 313,680 - GFF317 0.76 -0.8 313,744 - GFF317 0.82 +1.2 313,751 + GFF317 0.82 -0.3 313,753 + GFF317 0.82 -1.3 313,756 + GFF317 0.83 -0.3 313,758 + GFF317 0.83 +0.8 313,758 + GFF317 0.83 +0.2 313,759 - GFF317 0.83 -0.6 313,766 - GFF317 0.83 +0.9 313,766 - GFF317 0.83 +0.8 313,766 - GFF317 0.83 -1.2 313,785 - GFF317 0.85 +0.6 313,796 - GFF317 0.86 -0.1 313,806 + GFF317 0.87 -0.1 313,806 + GFF317 0.87 -0.7 313,814 - GFF317 0.87 -0.2 313,814 - GFF317 0.87 -0.1 313,820 + GFF317 0.88 +0.8 313,828 - GFF317 0.88 +0.3 313,828 - GFF317 0.88 -0.0 313,833 - GFF317 0.89 +0.8 313,838 + GFF317 0.89 -0.2 313,848 + -0.3 313,856 - +0.0 313,856 - -2.4 313,878 + +0.2 313,881 + -1.2 313,891 + +0.3 313,891 + -0.6 313,904 - +0.0 313,912 - -0.2 313,926 - +0.6 313,931 + -0.8
Or see this region's nucleotide sequence