Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF216 and GFF217 overlap by 4 nucleotides GFF217 and GFF218 overlap by 4 nucleotides GFF218 and GFF219 are separated by 213 nucleotides GFF219 and GFF220 are separated by 83 nucleotides GFF220 and GFF221 are separated by 56 nucleotides
GFF216 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), N-terminal domain, at 223,430 to 224,476
GFF216
GFF217 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 224,473 to 225,405
GFF217
GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373
GFF218
GFF219 - hypothetical protein, at 226,587 to 226,805
GFF219
GFF220 - hypothetical protein, at 226,889 to 227,122
GFF220
GFF221 - hypothetical protein, at 227,179 to 227,604
GFF221
Position (kb) 225
226
227 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 224.406 kb on + strand at 224.408 kb on + strand at 224.416 kb on - strand at 224.434 kb on - strand at 224.480 kb on + strand at 224.480 kb on - strand at 224.488 kb on - strand at 224.488 kb on - strand at 224.488 kb on - strand at 224.495 kb on + strand at 224.495 kb on + strand at 224.503 kb on - strand at 224.514 kb on + strand at 224.514 kb on + strand at 224.515 kb on + strand at 224.522 kb on - strand at 224.522 kb on - strand at 224.522 kb on - strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.540 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.549 kb on + strand at 224.559 kb on + strand at 224.561 kb on - strand at 224.588 kb on + strand, within GFF217 at 224.608 kb on - strand, within GFF217 at 224.618 kb on - strand, within GFF217 at 224.658 kb on + strand, within GFF217 at 224.666 kb on - strand, within GFF217 at 224.666 kb on - strand, within GFF217 at 224.690 kb on - strand, within GFF217 at 224.697 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.730 kb on - strand, within GFF217 at 224.797 kb on - strand, within GFF217 at 224.836 kb on + strand, within GFF217 at 224.844 kb on - strand, within GFF217 at 224.844 kb on - strand, within GFF217 at 224.863 kb on + strand, within GFF217 at 224.899 kb on - strand, within GFF217 at 224.963 kb on + strand, within GFF217 at 224.975 kb on + strand, within GFF217 at 224.975 kb on + strand, within GFF217 at 224.975 kb on + strand, within GFF217 at 224.983 kb on - strand, within GFF217 at 224.983 kb on - strand, within GFF217 at 224.998 kb on + strand, within GFF217 at 224.998 kb on - strand, within GFF217 at 225.005 kb on + strand, within GFF217 at 225.026 kb on - strand, within GFF217 at 225.029 kb on + strand, within GFF217 at 225.029 kb on + strand, within GFF217 at 225.037 kb on - strand, within GFF217 at 225.037 kb on - strand, within GFF217 at 225.047 kb on + strand, within GFF217 at 225.065 kb on - strand, within GFF217 at 225.078 kb on - strand, within GFF217 at 225.100 kb on - strand, within GFF217 at 225.100 kb on - strand, within GFF217 at 225.104 kb on + strand, within GFF217 at 225.110 kb on + strand, within GFF217 at 225.121 kb on + strand, within GFF217 at 225.136 kb on + strand, within GFF217 at 225.136 kb on + strand, within GFF217 at 225.136 kb on + strand, within GFF217 at 225.144 kb on - strand, within GFF217 at 225.144 kb on - strand, within GFF217 at 225.188 kb on + strand, within GFF217 at 225.188 kb on + strand, within GFF217 at 225.189 kb on + strand, within GFF217 at 225.189 kb on + strand, within GFF217 at 225.197 kb on - strand, within GFF217 at 225.197 kb on - strand, within GFF217 at 225.222 kb on + strand, within GFF217 at 225.222 kb on + strand, within GFF217 at 225.222 kb on + strand, within GFF217 at 225.222 kb on + strand, within GFF217 at 225.230 kb on - strand, within GFF217 at 225.230 kb on - strand, within GFF217 at 225.230 kb on - strand, within GFF217 at 225.232 kb on - strand, within GFF217 at 225.278 kb on + strand, within GFF217 at 225.283 kb on + strand, within GFF217 at 225.286 kb on - strand, within GFF217 at 225.346 kb on + strand at 225.346 kb on + strand at 225.351 kb on + strand at 225.359 kb on - strand at 225.361 kb on + strand at 225.374 kb on + strand at 225.394 kb on + strand at 225.395 kb on + strand at 225.397 kb on - strand at 225.403 kb on - strand at 225.419 kb on + strand at 225.462 kb on + strand at 225.476 kb on + strand at 225.484 kb on - strand at 225.491 kb on + strand at 225.509 kb on + strand, within GFF218 at 225.512 kb on - strand, within GFF218 at 225.514 kb on + strand, within GFF218 at 225.565 kb on - strand, within GFF218 at 225.566 kb on - strand, within GFF218 at 225.566 kb on - strand, within GFF218 at 225.580 kb on + strand, within GFF218 at 225.582 kb on + strand, within GFF218 at 225.583 kb on - strand, within GFF218 at 225.640 kb on + strand, within GFF218 at 225.642 kb on + strand, within GFF218 at 225.664 kb on + strand, within GFF218 at 225.672 kb on - strand, within GFF218 at 225.677 kb on - strand, within GFF218 at 225.689 kb on - strand, within GFF218 at 225.696 kb on - strand, within GFF218 at 225.708 kb on + strand, within GFF218 at 225.713 kb on + strand, within GFF218 at 225.716 kb on - strand, within GFF218 at 225.716 kb on - strand, within GFF218 at 225.718 kb on - strand, within GFF218 at 225.719 kb on + strand, within GFF218 at 225.721 kb on - strand, within GFF218 at 225.736 kb on + strand, within GFF218 at 225.765 kb on - strand, within GFF218 at 225.778 kb on - strand, within GFF218 at 225.798 kb on - strand, within GFF218 at 225.800 kb on + strand, within GFF218 at 225.852 kb on - strand, within GFF218 at 225.933 kb on + strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.973 kb on + strand, within GFF218 at 226.026 kb on + strand, within GFF218 at 226.034 kb on - strand, within GFF218 at 226.071 kb on + strand, within GFF218 at 226.075 kb on - strand, within GFF218 at 226.079 kb on - strand, within GFF218 at 226.094 kb on - strand, within GFF218 at 226.129 kb on - strand, within GFF218 at 226.141 kb on + strand, within GFF218 at 226.148 kb on - strand, within GFF218 at 226.148 kb on - strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.249 kb on + strand, within GFF218 at 226.295 kb on - strand at 226.300 kb on + strand at 226.308 kb on - strand at 226.318 kb on - strand at 226.353 kb on - strand at 226.450 kb on + strand at 226.458 kb on - strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.483 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.490 kb on + strand at 226.490 kb on + strand at 226.498 kb on - strand at 226.498 kb on - strand at 226.498 kb on - strand at 226.558 kb on - strand at 226.578 kb on + strand at 226.578 kb on + strand at 226.585 kb on - strand at 226.589 kb on + strand at 226.596 kb on - strand at 226.639 kb on - strand, within GFF219 at 226.639 kb on - strand, within GFF219 at 226.639 kb on - strand, within GFF219 at 226.671 kb on + strand, within GFF219 at 226.679 kb on - strand, within GFF219 at 226.692 kb on + strand, within GFF219 at 226.692 kb on + strand, within GFF219 at 226.700 kb on - strand, within GFF219 at 226.700 kb on - strand, within GFF219 at 226.747 kb on - strand, within GFF219 at 226.748 kb on + strand, within GFF219 at 226.843 kb on - strand at 226.861 kb on + strand at 226.887 kb on - strand at 226.904 kb on + strand at 226.907 kb on - strand at 226.907 kb on - strand at 226.920 kb on - strand, within GFF220 at 226.933 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.990 kb on + strand, within GFF220 at 226.993 kb on + strand, within GFF220 at 227.001 kb on - strand, within GFF220 at 227.060 kb on - strand, within GFF220 at 227.061 kb on - strand, within GFF220 at 227.068 kb on + strand, within GFF220 at 227.085 kb on - strand, within GFF220 at 227.104 kb on + strand at 227.112 kb on - strand at 227.152 kb on + strand at 227.160 kb on - strand at 227.160 kb on - strand at 227.170 kb on + strand at 227.175 kb on - strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.270 kb on + strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.286 kb on + strand, within GFF221 at 227.294 kb on - strand, within GFF221 at 227.301 kb on + strand, within GFF221 at 227.326 kb on + strand, within GFF221 at 227.326 kb on - strand, within GFF221 at 227.334 kb on - strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.343 kb on + strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.351 kb on - strand, within GFF221
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 224,406 + -2.2 224,408 + -1.3 224,416 - -3.9 224,434 - -2.4 224,480 + -2.5 224,480 - -1.0 224,488 - +0.7 224,488 - +0.8 224,488 - +0.2 224,495 + -2.5 224,495 + -1.5 224,503 - -2.1 224,514 + -2.2 224,514 + -2.2 224,515 + -1.2 224,522 - +1.4 224,522 - -1.3 224,522 - +0.8 224,539 + -2.2 224,539 + -2.7 224,539 + -2.2 224,539 + -2.8 224,539 + -2.4 224,539 + -1.9 224,540 - -1.9 224,547 - -1.6 224,547 - -2.4 224,547 - -2.4 224,547 - -1.7 224,547 - -1.9 224,547 - -1.5 224,547 - -1.9 224,547 - -1.4 224,547 - -1.4 224,547 - -1.2 224,547 - -1.9 224,549 + -1.4 224,559 + -1.2 224,561 - -1.2 224,588 + GFF217 0.12 -1.9 224,608 - GFF217 0.14 -0.5 224,618 - GFF217 0.16 -2.4 224,658 + GFF217 0.20 -2.0 224,666 - GFF217 0.21 -2.8 224,666 - GFF217 0.21 -3.8 224,690 - GFF217 0.23 -2.0 224,697 + GFF217 0.24 -1.6 224,702 + GFF217 0.25 -2.9 224,702 + GFF217 0.25 -3.5 224,702 + GFF217 0.25 -2.7 224,702 + GFF217 0.25 -1.9 224,702 + GFF217 0.25 -3.5 224,702 + GFF217 0.25 -2.0 224,710 - GFF217 0.25 -2.6 224,710 - GFF217 0.25 -1.7 224,710 - GFF217 0.25 -2.6 224,710 - GFF217 0.25 -1.5 224,730 - GFF217 0.28 -0.3 224,797 - GFF217 0.35 -1.6 224,836 + GFF217 0.39 -2.1 224,844 - GFF217 0.40 -1.8 224,844 - GFF217 0.40 -1.8 224,863 + GFF217 0.42 -3.1 224,899 - GFF217 0.46 -1.3 224,963 + GFF217 0.53 -2.4 224,975 + GFF217 0.54 -3.8 224,975 + GFF217 0.54 -1.7 224,975 + GFF217 0.54 -1.5 224,983 - GFF217 0.55 -2.5 224,983 - GFF217 0.55 -1.5 224,998 + GFF217 0.56 -2.6 224,998 - GFF217 0.56 -2.0 225,005 + GFF217 0.57 -1.2 225,026 - GFF217 0.59 -1.3 225,029 + GFF217 0.60 -0.3 225,029 + GFF217 0.60 -1.2 225,037 - GFF217 0.60 -2.0 225,037 - GFF217 0.60 -2.0 225,047 + GFF217 0.62 -3.2 225,065 - GFF217 0.63 -2.1 225,078 - GFF217 0.65 -2.2 225,100 - GFF217 0.67 -2.5 225,100 - GFF217 0.67 -1.3 225,104 + GFF217 0.68 -3.3 225,110 + GFF217 0.68 -1.4 225,121 + GFF217 0.69 -2.3 225,136 + GFF217 0.71 -1.8 225,136 + GFF217 0.71 -1.5 225,136 + GFF217 0.71 -2.0 225,144 - GFF217 0.72 -2.7 225,144 - GFF217 0.72 -3.4 225,188 + GFF217 0.77 -3.0 225,188 + GFF217 0.77 -2.0 225,189 + GFF217 0.77 -1.6 225,189 + GFF217 0.77 -2.4 225,197 - GFF217 0.78 -2.7 225,197 - GFF217 0.78 -2.4 225,222 + GFF217 0.80 -2.8 225,222 + GFF217 0.80 -3.0 225,222 + GFF217 0.80 -3.0 225,222 + GFF217 0.80 -1.3 225,230 - GFF217 0.81 -2.5 225,230 - GFF217 0.81 -1.7 225,230 - GFF217 0.81 -2.9 225,232 - GFF217 0.81 -1.2 225,278 + GFF217 0.86 -2.8 225,283 + GFF217 0.87 -2.2 225,286 - GFF217 0.87 -4.0 225,346 + -1.2 225,346 + -1.8 225,351 + -1.4 225,359 - -1.8 225,361 + -1.5 225,374 + -2.7 225,394 + -2.2 225,395 + -1.2 225,397 - -1.7 225,403 - -2.2 225,419 + -0.3 225,462 + -0.7 225,476 + -1.1 225,484 - -1.2 225,491 + -1.3 225,509 + GFF218 0.11 -1.4 225,512 - GFF218 0.11 -0.3 225,514 + GFF218 0.12 -0.8 225,565 - GFF218 0.17 -0.4 225,566 - GFF218 0.17 -0.8 225,566 - GFF218 0.17 +0.1 225,580 + GFF218 0.18 -0.7 225,582 + GFF218 0.19 -0.6 225,583 - GFF218 0.19 +0.1 225,640 + GFF218 0.24 -1.3 225,642 + GFF218 0.25 -1.3 225,664 + GFF218 0.27 -1.1 225,672 - GFF218 0.28 -1.2 225,677 - GFF218 0.28 -0.1 225,689 - GFF218 0.30 +0.3 225,696 - GFF218 0.30 -0.4 225,708 + GFF218 0.31 +0.8 225,713 + GFF218 0.32 -1.6 225,716 - GFF218 0.32 +0.5 225,716 - GFF218 0.32 +0.6 225,718 - GFF218 0.33 +1.7 225,719 + GFF218 0.33 -0.4 225,721 - GFF218 0.33 +1.0 225,736 + GFF218 0.34 +1.0 225,765 - GFF218 0.37 -0.8 225,778 - GFF218 0.39 +0.6 225,798 - GFF218 0.41 +0.2 225,800 + GFF218 0.41 -2.2 225,852 - GFF218 0.46 -0.4 225,933 + GFF218 0.55 -1.9 225,941 - GFF218 0.55 -0.9 225,941 - GFF218 0.55 -0.9 225,941 - GFF218 0.55 -2.2 225,973 + GFF218 0.59 -0.6 226,026 + GFF218 0.64 -2.4 226,034 - GFF218 0.65 -0.5 226,071 + GFF218 0.69 -0.3 226,075 - GFF218 0.69 -2.0 226,079 - GFF218 0.70 -0.4 226,094 - GFF218 0.71 +0.4 226,129 - GFF218 0.75 -0.0 226,141 + GFF218 0.76 -0.5 226,148 - GFF218 0.77 -0.2 226,148 - GFF218 0.77 -1.8 226,154 + GFF218 0.77 +0.0 226,154 + GFF218 0.77 -1.4 226,154 + GFF218 0.77 -0.2 226,249 + GFF218 0.87 -0.1 226,295 - -2.0 226,300 + -1.4 226,308 - -0.0 226,318 - -1.1 226,353 - -0.3 226,450 + +1.1 226,458 - +0.2 226,480 + -0.6 226,480 + -1.2 226,480 + +0.7 226,480 + -0.5 226,480 + -0.1 226,480 + +1.5 226,483 - +0.8 226,488 - +0.3 226,488 - +0.6 226,488 - +1.4 226,488 - -0.2 226,488 - -1.1 226,488 - -1.0 226,488 - -0.3 226,490 + +0.4 226,490 + +0.3 226,498 - -0.8 226,498 - +2.0 226,498 - +0.4 226,558 - +0.0 226,578 + +1.9 226,578 + +0.8 226,585 - +0.2 226,589 + +0.6 226,596 - -1.2 226,639 - GFF219 0.24 +0.5 226,639 - GFF219 0.24 -1.2 226,639 - GFF219 0.24 +1.4 226,671 + GFF219 0.38 -1.2 226,679 - GFF219 0.42 -0.3 226,692 + GFF219 0.48 -0.4 226,692 + GFF219 0.48 -0.1 226,700 - GFF219 0.52 -1.2 226,700 - GFF219 0.52 -1.8 226,747 - GFF219 0.73 +0.1 226,748 + GFF219 0.74 -0.4 226,843 - +0.5 226,861 + +0.4 226,887 - -0.3 226,904 + +0.9 226,907 - -1.7 226,907 - +0.3 226,920 - GFF220 0.13 +0.8 226,933 + GFF220 0.19 +0.5 226,948 + GFF220 0.25 +1.4 226,948 + GFF220 0.25 -0.5 226,976 + GFF220 0.37 +0.3 226,976 + GFF220 0.37 -0.6 226,976 + GFF220 0.37 +1.2 226,976 + GFF220 0.37 +0.3 226,976 + GFF220 0.37 -0.2 226,976 + GFF220 0.37 +0.6 226,976 + GFF220 0.37 -0.6 226,976 + GFF220 0.37 -1.1 226,976 + GFF220 0.37 +0.0 226,984 - GFF220 0.41 +0.4 226,984 - GFF220 0.41 +1.2 226,984 - GFF220 0.41 -0.9 226,984 - GFF220 0.41 -1.1 226,984 - GFF220 0.41 +0.2 226,984 - GFF220 0.41 -0.2 226,990 + GFF220 0.43 -2.2 226,993 + GFF220 0.44 +0.3 227,001 - GFF220 0.48 -0.0 227,060 - GFF220 0.73 +0.1 227,061 - GFF220 0.74 -1.2 227,068 + GFF220 0.76 -1.0 227,085 - GFF220 0.84 -0.9 227,104 + -0.8 227,112 - -1.5 227,152 + -0.3 227,160 - +0.1 227,160 - -2.2 227,170 + -0.2 227,175 - -1.5 227,177 + -0.7 227,177 + +0.4 227,177 + +0.1 227,185 - -0.2 227,185 - +0.1 227,185 - +0.3 227,270 + GFF221 0.21 +0.4 227,283 - GFF221 0.24 -0.9 227,283 - GFF221 0.24 +0.6 227,286 + GFF221 0.25 -0.3 227,294 - GFF221 0.27 -0.9 227,301 + GFF221 0.29 -0.2 227,326 + GFF221 0.35 -0.7 227,326 - GFF221 0.35 +0.1 227,334 - GFF221 0.36 +0.7 227,339 + GFF221 0.38 -0.7 227,339 + GFF221 0.38 -0.6 227,339 + GFF221 0.38 +0.0 227,343 + GFF221 0.38 -1.5 227,351 - GFF221 0.40 +0.8 227,351 - GFF221 0.40 -0.6
Or see this region's nucleotide sequence