Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1891 and GFF1892 are separated by 24 nucleotides GFF1892 and GFF1893 are separated by 41 nucleotides GFF1893 and GFF1894 are separated by 123 nucleotides
GFF1891 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 1,915,371 to 1,916,504
GFF1891
GFF1892 - Nucleoside-diphosphate-sugar epimerase, at 1,916,529 to 1,917,425
GFF1892
GFF1893 - hypothetical protein, at 1,917,467 to 1,917,772
GFF1893
GFF1894 - Colicin V production protein, at 1,917,896 to 1,918,420
GFF1894
Position (kb)
1916
1917
1918 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1915.533 kb on - strand, within GFF1891 at 1915.540 kb on - strand, within GFF1891 at 1915.576 kb on + strand, within GFF1891 at 1915.584 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.605 kb on + strand, within GFF1891 at 1915.757 kb on + strand, within GFF1891 at 1915.814 kb on - strand, within GFF1891 at 1915.902 kb on - strand, within GFF1891 at 1915.904 kb on - strand, within GFF1891 at 1915.904 kb on - strand, within GFF1891 at 1915.912 kb on - strand, within GFF1891 at 1915.914 kb on - strand, within GFF1891 at 1915.914 kb on - strand, within GFF1891 at 1915.921 kb on + strand, within GFF1891 at 1915.922 kb on + strand, within GFF1891 at 1915.930 kb on - strand, within GFF1891 at 1915.930 kb on - strand, within GFF1891 at 1915.937 kb on + strand, within GFF1891 at 1915.938 kb on - strand, within GFF1891 at 1915.962 kb on - strand, within GFF1891 at 1915.964 kb on - strand, within GFF1891 at 1915.969 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1916.006 kb on + strand, within GFF1891 at 1916.014 kb on - strand, within GFF1891 at 1916.022 kb on + strand, within GFF1891 at 1916.030 kb on - strand, within GFF1891 at 1916.030 kb on - strand, within GFF1891 at 1916.034 kb on + strand, within GFF1891 at 1916.034 kb on + strand, within GFF1891 at 1916.057 kb on - strand, within GFF1891 at 1916.065 kb on - strand, within GFF1891 at 1916.067 kb on + strand, within GFF1891 at 1916.075 kb on - strand, within GFF1891 at 1916.117 kb on + strand, within GFF1891 at 1916.198 kb on + strand, within GFF1891 at 1916.262 kb on + strand, within GFF1891 at 1916.267 kb on + strand, within GFF1891 at 1916.267 kb on + strand, within GFF1891 at 1916.267 kb on + strand, within GFF1891 at 1916.274 kb on + strand, within GFF1891 at 1916.281 kb on + strand, within GFF1891 at 1916.281 kb on - strand, within GFF1891 at 1916.281 kb on - strand, within GFF1891 at 1916.293 kb on - strand, within GFF1891 at 1916.306 kb on + strand, within GFF1891 at 1916.306 kb on + strand, within GFF1891 at 1916.306 kb on + strand, within GFF1891 at 1916.306 kb on + strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.314 kb on - strand, within GFF1891 at 1916.316 kb on + strand, within GFF1891 at 1916.322 kb on - strand, within GFF1891 at 1916.358 kb on + strand, within GFF1891 at 1916.371 kb on + strand, within GFF1891 at 1916.371 kb on + strand, within GFF1891 at 1916.379 kb on - strand, within GFF1891 at 1916.379 kb on - strand, within GFF1891 at 1916.379 kb on - strand, within GFF1891 at 1916.385 kb on + strand, within GFF1891 at 1916.385 kb on + strand, within GFF1891 at 1916.435 kb on + strand at 1916.437 kb on + strand at 1916.437 kb on + strand at 1916.437 kb on + strand at 1916.443 kb on - strand at 1916.445 kb on - strand at 1916.459 kb on + strand at 1916.459 kb on + strand at 1916.459 kb on + strand at 1916.459 kb on + strand at 1916.467 kb on - strand at 1916.467 kb on - strand at 1916.467 kb on - strand at 1916.469 kb on + strand at 1916.477 kb on - strand at 1916.487 kb on - strand at 1916.498 kb on + strand at 1916.506 kb on - strand at 1916.506 kb on - strand at 1916.506 kb on - strand at 1916.511 kb on - strand at 1916.516 kb on - strand at 1916.534 kb on - strand at 1916.563 kb on + strand at 1916.587 kb on + strand at 1916.594 kb on + strand at 1916.619 kb on + strand, within GFF1892 at 1916.619 kb on + strand, within GFF1892 at 1916.624 kb on + strand, within GFF1892 at 1916.634 kb on + strand, within GFF1892 at 1916.749 kb on + strand, within GFF1892 at 1916.779 kb on - strand, within GFF1892 at 1916.786 kb on + strand, within GFF1892 at 1916.786 kb on + strand, within GFF1892 at 1916.786 kb on + strand, within GFF1892 at 1916.794 kb on - strand, within GFF1892 at 1916.794 kb on - strand, within GFF1892 at 1916.794 kb on - strand, within GFF1892 at 1916.823 kb on + strand, within GFF1892 at 1916.831 kb on - strand, within GFF1892 at 1916.865 kb on + strand, within GFF1892 at 1916.865 kb on + strand, within GFF1892 at 1916.868 kb on - strand, within GFF1892 at 1916.873 kb on - strand, within GFF1892 at 1916.873 kb on - strand, within GFF1892 at 1916.883 kb on - strand, within GFF1892 at 1916.889 kb on + strand, within GFF1892 at 1916.901 kb on + strand, within GFF1892 at 1916.906 kb on + strand, within GFF1892 at 1916.922 kb on - strand, within GFF1892 at 1916.961 kb on - strand, within GFF1892 at 1916.971 kb on + strand, within GFF1892 at 1916.980 kb on - strand, within GFF1892 at 1916.981 kb on + strand, within GFF1892 at 1916.989 kb on - strand, within GFF1892 at 1917.003 kb on + strand, within GFF1892 at 1917.009 kb on - strand, within GFF1892 at 1917.015 kb on - strand, within GFF1892 at 1917.038 kb on + strand, within GFF1892 at 1917.068 kb on + strand, within GFF1892 at 1917.076 kb on - strand, within GFF1892 at 1917.117 kb on - strand, within GFF1892 at 1917.190 kb on - strand, within GFF1892 at 1917.192 kb on + strand, within GFF1892 at 1917.192 kb on + strand, within GFF1892 at 1917.192 kb on + strand, within GFF1892 at 1917.199 kb on + strand, within GFF1892 at 1917.200 kb on - strand, within GFF1892 at 1917.278 kb on - strand, within GFF1892 at 1917.285 kb on - strand, within GFF1892 at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.347 kb on + strand at 1917.352 kb on + strand at 1917.355 kb on - strand at 1917.355 kb on - strand at 1917.380 kb on + strand at 1917.380 kb on + strand at 1917.421 kb on - strand at 1917.443 kb on - strand at 1917.494 kb on + strand at 1917.525 kb on + strand, within GFF1893 at 1917.551 kb on - strand, within GFF1893 at 1917.551 kb on - strand, within GFF1893 at 1917.586 kb on + strand, within GFF1893 at 1917.602 kb on - strand, within GFF1893 at 1917.607 kb on - strand, within GFF1893 at 1917.672 kb on - strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.689 kb on + strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.697 kb on - strand, within GFF1893 at 1917.708 kb on + strand, within GFF1893 at 1917.708 kb on + strand, within GFF1893 at 1917.708 kb on + strand, within GFF1893 at 1917.708 kb on + strand, within GFF1893 at 1917.708 kb on + strand, within GFF1893 at 1917.713 kb on + strand, within GFF1893 at 1917.716 kb on - strand, within GFF1893 at 1917.716 kb on - strand, within GFF1893 at 1917.716 kb on - strand, within GFF1893 at 1917.716 kb on - strand, within GFF1893 at 1917.728 kb on + strand, within GFF1893 at 1917.728 kb on + strand, within GFF1893 at 1917.731 kb on - strand, within GFF1893 at 1917.736 kb on - strand, within GFF1893 at 1917.749 kb on + strand at 1917.883 kb on - strand at 1917.883 kb on - strand at 1917.883 kb on - strand at 1917.885 kb on + strand at 1917.906 kb on + strand at 1917.906 kb on + strand at 1917.906 kb on + strand at 1917.946 kb on - strand at 1917.982 kb on + strand, within GFF1894 at 1917.990 kb on - strand, within GFF1894 at 1918.051 kb on - strand, within GFF1894 at 1918.070 kb on + strand, within GFF1894 at 1918.070 kb on + strand, within GFF1894 at 1918.085 kb on + strand, within GFF1894 at 1918.090 kb on + strand, within GFF1894 at 1918.120 kb on + strand, within GFF1894 at 1918.147 kb on + strand, within GFF1894 at 1918.175 kb on + strand, within GFF1894 at 1918.223 kb on + strand, within GFF1894 at 1918.231 kb on - strand, within GFF1894 at 1918.236 kb on + strand, within GFF1894 at 1918.236 kb on + strand, within GFF1894 at 1918.239 kb on - strand, within GFF1894 at 1918.284 kb on + strand, within GFF1894
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,915,533 - GFF1891 0.14 -2.2 1,915,540 - GFF1891 0.15 -2.0 1,915,576 + GFF1891 0.18 -3.2 1,915,584 + GFF1891 0.19 -2.9 1,915,586 + GFF1891 0.19 -3.0 1,915,594 - GFF1891 0.20 -2.5 1,915,594 - GFF1891 0.20 -2.4 1,915,605 + GFF1891 0.21 +0.1 1,915,757 + GFF1891 0.34 -2.1 1,915,814 - GFF1891 0.39 -2.7 1,915,902 - GFF1891 0.47 -2.6 1,915,904 - GFF1891 0.47 -2.0 1,915,904 - GFF1891 0.47 -3.3 1,915,912 - GFF1891 0.48 -2.8 1,915,914 - GFF1891 0.48 -1.7 1,915,914 - GFF1891 0.48 -1.6 1,915,921 + GFF1891 0.49 +0.4 1,915,922 + GFF1891 0.49 -2.3 1,915,930 - GFF1891 0.49 -2.2 1,915,930 - GFF1891 0.49 -0.7 1,915,937 + GFF1891 0.50 -2.7 1,915,938 - GFF1891 0.50 -3.0 1,915,962 - GFF1891 0.52 -1.3 1,915,964 - GFF1891 0.52 -3.4 1,915,969 + GFF1891 0.53 -3.4 1,915,972 + GFF1891 0.53 -2.8 1,915,972 + GFF1891 0.53 -3.0 1,915,980 - GFF1891 0.54 -3.3 1,915,980 - GFF1891 0.54 -1.3 1,915,980 - GFF1891 0.54 -1.9 1,915,980 - GFF1891 0.54 -1.1 1,916,006 + GFF1891 0.56 -2.6 1,916,014 - GFF1891 0.57 -2.8 1,916,022 + GFF1891 0.57 -0.1 1,916,030 - GFF1891 0.58 -0.9 1,916,030 - GFF1891 0.58 -3.1 1,916,034 + GFF1891 0.58 -2.7 1,916,034 + GFF1891 0.58 -3.3 1,916,057 - GFF1891 0.60 -0.4 1,916,065 - GFF1891 0.61 -2.4 1,916,067 + GFF1891 0.61 -2.5 1,916,075 - GFF1891 0.62 +0.1 1,916,117 + GFF1891 0.66 -1.1 1,916,198 + GFF1891 0.73 -0.4 1,916,262 + GFF1891 0.79 -2.0 1,916,267 + GFF1891 0.79 -3.1 1,916,267 + GFF1891 0.79 -2.5 1,916,267 + GFF1891 0.79 -1.5 1,916,274 + GFF1891 0.80 -0.6 1,916,281 + GFF1891 0.80 +0.1 1,916,281 - GFF1891 0.80 -1.3 1,916,281 - GFF1891 0.80 -1.9 1,916,293 - GFF1891 0.81 -3.6 1,916,306 + GFF1891 0.82 -2.2 1,916,306 + GFF1891 0.82 -2.8 1,916,306 + GFF1891 0.82 -2.9 1,916,306 + GFF1891 0.82 -1.9 1,916,314 - GFF1891 0.83 -3.7 1,916,314 - GFF1891 0.83 -1.5 1,916,314 - GFF1891 0.83 +0.4 1,916,314 - GFF1891 0.83 -1.4 1,916,314 - GFF1891 0.83 -1.9 1,916,314 - GFF1891 0.83 -1.6 1,916,314 - GFF1891 0.83 -2.0 1,916,314 - GFF1891 0.83 -1.9 1,916,314 - GFF1891 0.83 -1.1 1,916,316 + GFF1891 0.83 -1.6 1,916,322 - GFF1891 0.84 -2.1 1,916,358 + GFF1891 0.87 -1.3 1,916,371 + GFF1891 0.88 -1.5 1,916,371 + GFF1891 0.88 -1.9 1,916,379 - GFF1891 0.89 -1.9 1,916,379 - GFF1891 0.89 -1.9 1,916,379 - GFF1891 0.89 -0.3 1,916,385 + GFF1891 0.89 -2.4 1,916,385 + GFF1891 0.89 -1.9 1,916,435 + -1.5 1,916,437 + +0.4 1,916,437 + -2.0 1,916,437 + -2.5 1,916,443 - -1.6 1,916,445 - -0.3 1,916,459 + -2.5 1,916,459 + -3.5 1,916,459 + -1.0 1,916,459 + -2.6 1,916,467 - -2.5 1,916,467 - -2.0 1,916,467 - -1.3 1,916,469 + -2.0 1,916,477 - -2.7 1,916,487 - -1.7 1,916,498 + +1.0 1,916,506 - -2.6 1,916,506 - +0.8 1,916,506 - +0.8 1,916,511 - +0.2 1,916,516 - +0.0 1,916,534 - -1.4 1,916,563 + +0.1 1,916,587 + -0.3 1,916,594 + +0.2 1,916,619 + GFF1892 0.10 +0.8 1,916,619 + GFF1892 0.10 -2.1 1,916,624 + GFF1892 0.11 +0.0 1,916,634 + GFF1892 0.12 -0.5 1,916,749 + GFF1892 0.25 -1.3 1,916,779 - GFF1892 0.28 +0.9 1,916,786 + GFF1892 0.29 +0.3 1,916,786 + GFF1892 0.29 -0.6 1,916,786 + GFF1892 0.29 +0.2 1,916,794 - GFF1892 0.30 -0.0 1,916,794 - GFF1892 0.30 +0.9 1,916,794 - GFF1892 0.30 -0.6 1,916,823 + GFF1892 0.33 -0.8 1,916,831 - GFF1892 0.34 +0.6 1,916,865 + GFF1892 0.37 +0.3 1,916,865 + GFF1892 0.37 +0.1 1,916,868 - GFF1892 0.38 +1.0 1,916,873 - GFF1892 0.38 -0.3 1,916,873 - GFF1892 0.38 -0.3 1,916,883 - GFF1892 0.39 -0.5 1,916,889 + GFF1892 0.40 +0.4 1,916,901 + GFF1892 0.41 +0.1 1,916,906 + GFF1892 0.42 +0.4 1,916,922 - GFF1892 0.44 -1.5 1,916,961 - GFF1892 0.48 -0.9 1,916,971 + GFF1892 0.49 +0.3 1,916,980 - GFF1892 0.50 +0.9 1,916,981 + GFF1892 0.50 +1.3 1,916,989 - GFF1892 0.51 +0.4 1,917,003 + GFF1892 0.53 -1.0 1,917,009 - GFF1892 0.54 -0.9 1,917,015 - GFF1892 0.54 +1.0 1,917,038 + GFF1892 0.57 -0.7 1,917,068 + GFF1892 0.60 -0.3 1,917,076 - GFF1892 0.61 +0.2 1,917,117 - GFF1892 0.66 +0.0 1,917,190 - GFF1892 0.74 +1.2 1,917,192 + GFF1892 0.74 -0.3 1,917,192 + GFF1892 0.74 -1.1 1,917,192 + GFF1892 0.74 -0.5 1,917,199 + GFF1892 0.75 -0.1 1,917,200 - GFF1892 0.75 -1.1 1,917,278 - GFF1892 0.84 -0.5 1,917,285 - GFF1892 0.84 +0.6 1,917,347 + -0.8 1,917,347 + +0.4 1,917,347 + -0.9 1,917,347 + +1.0 1,917,347 + +0.4 1,917,347 + -0.1 1,917,347 + -1.1 1,917,347 + -1.0 1,917,347 + -0.4 1,917,347 + +0.5 1,917,352 + -1.8 1,917,355 - +0.7 1,917,355 - -0.8 1,917,380 + +0.1 1,917,380 + +0.8 1,917,421 - +1.6 1,917,443 - -0.1 1,917,494 + +0.1 1,917,525 + GFF1893 0.19 +1.2 1,917,551 - GFF1893 0.27 +1.1 1,917,551 - GFF1893 0.27 -2.2 1,917,586 + GFF1893 0.39 -1.4 1,917,602 - GFF1893 0.44 +0.5 1,917,607 - GFF1893 0.46 +0.1 1,917,672 - GFF1893 0.67 +0.8 1,917,689 + GFF1893 0.73 -0.9 1,917,689 + GFF1893 0.73 +0.9 1,917,689 + GFF1893 0.73 -1.0 1,917,689 + GFF1893 0.73 +1.4 1,917,689 + GFF1893 0.73 +0.7 1,917,689 + GFF1893 0.73 -0.9 1,917,689 + GFF1893 0.73 +0.4 1,917,689 + GFF1893 0.73 +0.4 1,917,689 + GFF1893 0.73 -0.2 1,917,689 + GFF1893 0.73 -0.6 1,917,689 + GFF1893 0.73 -0.5 1,917,689 + GFF1893 0.73 +2.4 1,917,689 + GFF1893 0.73 -0.3 1,917,689 + GFF1893 0.73 -0.6 1,917,697 - GFF1893 0.75 -0.6 1,917,697 - GFF1893 0.75 +0.9 1,917,697 - GFF1893 0.75 -0.5 1,917,697 - GFF1893 0.75 +0.3 1,917,697 - GFF1893 0.75 -0.3 1,917,697 - GFF1893 0.75 -1.9 1,917,697 - GFF1893 0.75 -0.8 1,917,697 - GFF1893 0.75 +0.4 1,917,697 - GFF1893 0.75 -0.6 1,917,708 + GFF1893 0.79 -0.6 1,917,708 + GFF1893 0.79 +1.5 1,917,708 + GFF1893 0.79 -0.6 1,917,708 + GFF1893 0.79 +2.0 1,917,708 + GFF1893 0.79 -0.9 1,917,713 + GFF1893 0.80 +0.4 1,917,716 - GFF1893 0.81 -1.0 1,917,716 - GFF1893 0.81 +0.0 1,917,716 - GFF1893 0.81 +0.4 1,917,716 - GFF1893 0.81 +1.3 1,917,728 + GFF1893 0.85 +0.6 1,917,728 + GFF1893 0.85 -0.4 1,917,731 - GFF1893 0.86 +0.1 1,917,736 - GFF1893 0.88 -0.6 1,917,749 + -0.4 1,917,883 - -0.1 1,917,883 - -0.9 1,917,883 - +0.6 1,917,885 + -0.5 1,917,906 + -1.5 1,917,906 + -1.0 1,917,906 + +0.1 1,917,946 - -0.1 1,917,982 + GFF1894 0.16 -0.1 1,917,990 - GFF1894 0.18 -0.3 1,918,051 - GFF1894 0.30 -1.9 1,918,070 + GFF1894 0.33 +1.7 1,918,070 + GFF1894 0.33 +0.2 1,918,085 + GFF1894 0.36 -0.2 1,918,090 + GFF1894 0.37 -1.5 1,918,120 + GFF1894 0.43 -0.4 1,918,147 + GFF1894 0.48 -0.5 1,918,175 + GFF1894 0.53 -0.2 1,918,223 + GFF1894 0.62 +0.5 1,918,231 - GFF1894 0.64 +0.4 1,918,236 + GFF1894 0.65 -1.0 1,918,236 + GFF1894 0.65 -1.0 1,918,239 - GFF1894 0.65 +0.6 1,918,284 + GFF1894 0.74 -0.5
Or see this region's nucleotide sequence