Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF133 and GFF134 overlap by 4 nucleotides GFF134 and GFF135 are separated by 226 nucleotides
GFF133 - Biotin synthase (EC 2.8.1.6), at 127,952 to 128,965
GFF133
GFF134 - hypothetical protein, at 128,962 to 129,078
GFF134
GFF135 - Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3), at 129,305 to 131,305
GFF135
Position (kb)
128
129
130 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 127.984 kb on + strand at 127.985 kb on + strand at 128.062 kb on - strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.100 kb on + strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.103 kb on - strand, within GFF133 at 128.127 kb on + strand, within GFF133 at 128.134 kb on + strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.148 kb on + strand, within GFF133 at 128.161 kb on - strand, within GFF133 at 128.191 kb on + strand, within GFF133 at 128.207 kb on - strand, within GFF133 at 128.218 kb on - strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.237 kb on + strand, within GFF133 at 128.247 kb on + strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.371 kb on - strand, within GFF133 at 128.430 kb on - strand, within GFF133 at 128.486 kb on + strand, within GFF133 at 128.486 kb on + strand, within GFF133 at 128.494 kb on - strand, within GFF133 at 128.555 kb on + strand, within GFF133 at 128.563 kb on - strand, within GFF133 at 128.572 kb on - strand, within GFF133 at 128.596 kb on + strand, within GFF133 at 128.600 kb on + strand, within GFF133 at 128.612 kb on + strand, within GFF133 at 128.615 kb on + strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.632 kb on - strand, within GFF133 at 128.655 kb on + strand, within GFF133 at 128.655 kb on - strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.711 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.742 kb on - strand, within GFF133 at 128.757 kb on + strand, within GFF133 at 128.762 kb on + strand, within GFF133 at 128.765 kb on - strand, within GFF133 at 128.767 kb on + strand, within GFF133 at 128.770 kb on - strand, within GFF133 at 128.770 kb on - strand, within GFF133 at 128.855 kb on + strand, within GFF133 at 128.873 kb on + strand at 128.908 kb on + strand at 128.908 kb on + strand at 128.918 kb on + strand at 128.960 kb on + strand at 128.970 kb on + strand at 128.973 kb on + strand at 129.008 kb on + strand, within GFF134 at 129.038 kb on - strand, within GFF134 at 129.038 kb on - strand, within GFF134 at 129.050 kb on - strand, within GFF134 at 129.061 kb on + strand, within GFF134 at 129.095 kb on + strand at 129.120 kb on + strand at 129.120 kb on + strand at 129.128 kb on - strand at 129.137 kb on + strand at 129.147 kb on + strand at 129.179 kb on - strand at 129.252 kb on - strand at 129.356 kb on + strand at 129.366 kb on + strand at 129.366 kb on + strand at 129.368 kb on + strand at 129.376 kb on - strand at 129.376 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.390 kb on - strand at 129.400 kb on - strand at 129.402 kb on + strand at 129.442 kb on + strand at 129.451 kb on + strand at 129.476 kb on + strand at 129.476 kb on + strand at 129.481 kb on + strand at 129.484 kb on - strand at 129.489 kb on - strand at 129.489 kb on - strand at 129.539 kb on + strand, within GFF135 at 129.708 kb on + strand, within GFF135 at 129.726 kb on + strand, within GFF135 at 129.732 kb on + strand, within GFF135 at 129.763 kb on - strand, within GFF135 at 129.773 kb on - strand, within GFF135 at 129.781 kb on + strand, within GFF135 at 129.781 kb on + strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.783 kb on - strand, within GFF135 at 129.826 kb on - strand, within GFF135 at 129.846 kb on + strand, within GFF135 at 129.854 kb on - strand, within GFF135 at 129.861 kb on + strand, within GFF135 at 129.877 kb on - strand, within GFF135 at 129.877 kb on - strand, within GFF135 at 129.961 kb on + strand, within GFF135 at 129.975 kb on + strand, within GFF135 at 130.034 kb on + strand, within GFF135 at 130.040 kb on + strand, within GFF135
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 127,984 + +1.2 127,985 + +0.4 128,062 - GFF133 0.11 -0.9 128,092 + GFF133 0.14 +1.0 128,092 + GFF133 0.14 -0.6 128,100 + GFF133 0.15 +0.5 128,100 - GFF133 0.15 +0.5 128,100 - GFF133 0.15 -0.6 128,103 - GFF133 0.15 +0.5 128,127 + GFF133 0.17 +1.6 128,134 + GFF133 0.18 -0.9 128,135 - GFF133 0.18 -1.6 128,135 - GFF133 0.18 +1.2 128,135 - GFF133 0.18 -0.0 128,135 - GFF133 0.18 +0.1 128,148 + GFF133 0.19 -0.2 128,161 - GFF133 0.21 -0.4 128,191 + GFF133 0.24 -0.2 128,207 - GFF133 0.25 +0.3 128,218 - GFF133 0.26 -1.0 128,230 + GFF133 0.27 -2.8 128,230 + GFF133 0.27 -0.0 128,230 + GFF133 0.27 +0.8 128,237 + GFF133 0.28 +0.6 128,247 + GFF133 0.29 +0.2 128,278 - GFF133 0.32 +0.1 128,278 - GFF133 0.32 -2.7 128,280 + GFF133 0.32 -0.4 128,280 + GFF133 0.32 -1.4 128,280 + GFF133 0.32 +0.3 128,317 + GFF133 0.36 -0.0 128,317 + GFF133 0.36 -0.4 128,317 + GFF133 0.36 +1.7 128,317 + GFF133 0.36 -0.0 128,325 - GFF133 0.37 -0.3 128,325 - GFF133 0.37 -1.1 128,325 - GFF133 0.37 -1.9 128,325 - GFF133 0.37 -0.7 128,325 - GFF133 0.37 -0.2 128,371 - GFF133 0.41 +0.5 128,430 - GFF133 0.47 -0.3 128,486 + GFF133 0.53 -0.3 128,486 + GFF133 0.53 +0.0 128,494 - GFF133 0.53 +1.1 128,555 + GFF133 0.59 -1.6 128,563 - GFF133 0.60 -0.5 128,572 - GFF133 0.61 +0.5 128,596 + GFF133 0.64 +0.8 128,600 + GFF133 0.64 -0.2 128,612 + GFF133 0.65 -0.4 128,615 + GFF133 0.65 -0.3 128,620 - GFF133 0.66 +1.4 128,620 - GFF133 0.66 -2.8 128,632 - GFF133 0.67 -0.2 128,655 + GFF133 0.69 -0.4 128,655 - GFF133 0.69 -0.4 128,660 + GFF133 0.70 -0.5 128,660 + GFF133 0.70 +0.6 128,660 + GFF133 0.70 -1.8 128,660 + GFF133 0.70 +0.1 128,660 + GFF133 0.70 -0.8 128,668 - GFF133 0.71 +1.0 128,668 - GFF133 0.71 -2.3 128,668 - GFF133 0.71 +0.8 128,668 - GFF133 0.71 -0.1 128,668 - GFF133 0.71 +1.2 128,668 - GFF133 0.71 +0.7 128,668 - GFF133 0.71 -1.7 128,668 - GFF133 0.71 -2.5 128,711 + GFF133 0.75 +0.3 128,724 + GFF133 0.76 -0.3 128,724 + GFF133 0.76 -0.2 128,742 - GFF133 0.78 +0.2 128,757 + GFF133 0.79 -0.7 128,762 + GFF133 0.80 -0.7 128,765 - GFF133 0.80 +1.0 128,767 + GFF133 0.80 +0.5 128,770 - GFF133 0.81 +0.1 128,770 - GFF133 0.81 -1.2 128,855 + GFF133 0.89 +0.3 128,873 + +0.9 128,908 + +1.1 128,908 + -0.4 128,918 + +2.0 128,960 + +0.5 128,970 + +0.3 128,973 + -0.3 129,008 + GFF134 0.39 -1.3 129,038 - GFF134 0.65 -0.2 129,038 - GFF134 0.65 -1.3 129,050 - GFF134 0.75 -1.4 129,061 + GFF134 0.85 +0.1 129,095 + -2.0 129,120 + -0.9 129,120 + -0.7 129,128 - -0.8 129,137 + +0.9 129,147 + -0.6 129,179 - -0.6 129,252 - +1.1 129,356 + -1.0 129,366 + +1.7 129,366 + -0.2 129,368 + +2.0 129,376 - +0.4 129,376 - -2.3 129,390 - -0.1 129,390 - -0.5 129,390 - +1.2 129,390 - +1.5 129,400 - -1.2 129,402 + -1.7 129,442 + +0.1 129,451 + +0.6 129,476 + +1.3 129,476 + +0.1 129,481 + +0.5 129,484 - -1.4 129,489 - -0.6 129,489 - +1.3 129,539 + GFF135 0.12 +0.0 129,708 + GFF135 0.20 +0.1 129,726 + GFF135 0.21 +1.4 129,732 + GFF135 0.21 +0.4 129,763 - GFF135 0.23 +0.7 129,773 - GFF135 0.23 +0.3 129,781 + GFF135 0.24 +0.1 129,781 + GFF135 0.24 +0.1 129,783 - GFF135 0.24 -0.2 129,783 - GFF135 0.24 -1.7 129,783 - GFF135 0.24 +0.1 129,826 - GFF135 0.26 -2.0 129,846 + GFF135 0.27 +1.5 129,854 - GFF135 0.27 -0.2 129,861 + GFF135 0.28 -0.4 129,877 - GFF135 0.29 -1.1 129,877 - GFF135 0.29 +0.8 129,961 + GFF135 0.33 +0.4 129,975 + GFF135 0.33 -0.4 130,034 + GFF135 0.36 +0.1 130,040 + GFF135 0.37 -0.0
Or see this region's nucleotide sequence