Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF822 and GFF823 are separated by 28 nucleotides GFF823 and GFF824 overlap by 4 nucleotides GFF824 and GFF825 overlap by 8 nucleotides GFF825 and GFF826 overlap by 4 nucleotides
GFF822 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 811,277 to 812,116
GFF822
GFF823 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 812,145 to 812,651
GFF823
GFF824 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 812,648 to 813,601
GFF824
GFF825 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 813,594 to 814,577
GFF825
GFF826 - Cobalamin synthase (EC 2.7.8.26), at 814,574 to 815,293
GFF826
Position (kb) 812
813
814 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 812.151 kb on + strand at 812.282 kb on - strand, within GFF823 at 812.301 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.554 kb on + strand, within GFF823 at 812.571 kb on + strand, within GFF823 at 812.639 kb on + strand at 812.659 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.696 kb on + strand at 812.708 kb on + strand at 812.710 kb on + strand at 812.710 kb on + strand at 812.710 kb on + strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.752 kb on + strand, within GFF824 at 812.782 kb on + strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.878 kb on + strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.975 kb on - strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 813.094 kb on + strand, within GFF824 at 813.319 kb on - strand, within GFF824 at 813.360 kb on - strand, within GFF824 at 813.368 kb on + strand, within GFF824 at 813.559 kb on - strand at 813.619 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.632 kb on - strand at 813.632 kb on - strand at 813.632 kb on - strand at 813.715 kb on + strand, within GFF825 at 813.779 kb on + strand, within GFF825 at 813.779 kb on + strand, within GFF825 at 813.853 kb on + strand, within GFF825 at 813.856 kb on - strand, within GFF825 at 813.913 kb on - strand, within GFF825 at 814.047 kb on - strand, within GFF825 at 814.047 kb on - strand, within GFF825 at 814.083 kb on + strand, within GFF825 at 814.188 kb on + strand, within GFF825 at 814.193 kb on + strand, within GFF825 at 814.207 kb on + strand, within GFF825 at 814.207 kb on + strand, within GFF825 at 814.211 kb on + strand, within GFF825 at 814.229 kb on - strand, within GFF825 at 814.242 kb on + strand, within GFF825 at 814.320 kb on + strand, within GFF825 at 814.325 kb on + strand, within GFF825 at 814.348 kb on + strand, within GFF825 at 814.351 kb on - strand, within GFF825 at 814.384 kb on + strand, within GFF825 at 814.501 kb on - strand at 814.573 kb on + strand at 814.579 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 812,151 + -1.6 812,282 - GFF823 0.27 -1.9 812,301 + GFF823 0.31 -2.3 812,419 + GFF823 0.54 -0.3 812,419 + GFF823 0.54 +0.2 812,419 + GFF823 0.54 -2.6 812,419 + GFF823 0.54 -1.4 812,419 + GFF823 0.54 -0.1 812,419 + GFF823 0.54 -0.9 812,427 - GFF823 0.56 +0.2 812,427 - GFF823 0.56 -2.3 812,427 - GFF823 0.56 -1.4 812,427 - GFF823 0.56 +0.5 812,427 - GFF823 0.56 -1.6 812,427 - GFF823 0.56 -2.6 812,427 - GFF823 0.56 -2.1 812,427 - GFF823 0.56 -0.6 812,427 - GFF823 0.56 -1.4 812,554 + GFF823 0.81 -1.1 812,571 + GFF823 0.84 -2.4 812,639 + +0.2 812,659 + +0.6 812,691 + +1.1 812,691 + +0.9 812,696 + -0.5 812,708 + -2.3 812,710 + -1.1 812,710 + +0.2 812,710 + -0.3 812,716 - -2.1 812,716 - -0.8 812,752 + GFF824 0.11 -0.1 812,782 + GFF824 0.14 -1.2 812,805 - GFF824 0.16 -2.1 812,805 - GFF824 0.16 -0.9 812,805 - GFF824 0.16 -0.8 812,838 - GFF824 0.20 -1.6 812,838 - GFF824 0.20 -0.5 812,838 - GFF824 0.20 -3.1 812,838 - GFF824 0.20 -2.9 812,878 + GFF824 0.24 -0.5 812,886 - GFF824 0.25 -1.7 812,886 - GFF824 0.25 -0.6 812,975 - GFF824 0.34 +0.2 812,985 - GFF824 0.35 -1.6 813,094 + GFF824 0.47 -0.3 813,319 - GFF824 0.70 +0.4 813,360 - GFF824 0.75 -0.3 813,368 + GFF824 0.75 -1.5 813,559 - -2.4 813,619 + -1.7 813,624 + +0.5 813,624 + -2.3 813,632 - -0.0 813,632 - -0.6 813,632 - -1.4 813,715 + GFF825 0.12 -0.6 813,779 + GFF825 0.19 -0.3 813,779 + GFF825 0.19 -1.8 813,853 + GFF825 0.26 -2.3 813,856 - GFF825 0.27 -2.0 813,913 - GFF825 0.32 -1.0 814,047 - GFF825 0.46 -0.9 814,047 - GFF825 0.46 -1.6 814,083 + GFF825 0.50 -2.3 814,188 + GFF825 0.60 -0.9 814,193 + GFF825 0.61 -1.6 814,207 + GFF825 0.62 -0.9 814,207 + GFF825 0.62 -2.2 814,211 + GFF825 0.63 -1.2 814,229 - GFF825 0.65 -0.0 814,242 + GFF825 0.66 -1.5 814,320 + GFF825 0.74 -0.8 814,325 + GFF825 0.74 -0.3 814,348 + GFF825 0.77 -1.4 814,351 - GFF825 0.77 -0.3 814,384 + GFF825 0.80 -1.0 814,501 - -1.0 814,573 + -0.5 814,579 - +0.3
Or see this region's nucleotide sequence