Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF821 and GFF822 overlap by 4 nucleotides GFF822 and GFF823 are separated by 28 nucleotides GFF823 and GFF824 overlap by 4 nucleotides GFF824 and GFF825 overlap by 8 nucleotides
GFF821 - 'Pyridoxal-5'-phosphate phosphatase (EC 3.1.3.74), Alphaproteobacterial type' transl_table=11, at 810,609 to 811,280
GFF821
GFF822 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 811,277 to 812,116
GFF822
GFF823 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 812,145 to 812,651
GFF823
GFF824 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 812,648 to 813,601
GFF824
GFF825 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 813,594 to 814,577
GFF825
Position (kb) 812
813 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 811.166 kb on - strand, within GFF821 at 811.180 kb on + strand, within GFF821 at 812.151 kb on + strand at 812.282 kb on - strand, within GFF823 at 812.301 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.554 kb on + strand, within GFF823 at 812.571 kb on + strand, within GFF823 at 812.639 kb on + strand at 812.659 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.696 kb on + strand at 812.708 kb on + strand at 812.710 kb on + strand at 812.710 kb on + strand at 812.710 kb on + strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.752 kb on + strand, within GFF824 at 812.782 kb on + strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.878 kb on + strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.975 kb on - strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 813.094 kb on + strand, within GFF824 at 813.319 kb on - strand, within GFF824 at 813.360 kb on - strand, within GFF824 at 813.368 kb on + strand, within GFF824 at 813.559 kb on - strand at 813.619 kb on + strand at 813.624 kb on + strand at 813.624 kb on + strand at 813.632 kb on - strand at 813.632 kb on - strand at 813.632 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 811,166 - GFF821 0.83 -1.8 811,180 + GFF821 0.85 +0.8 812,151 + -1.6 812,282 - GFF823 0.27 -1.9 812,301 + GFF823 0.31 -2.3 812,419 + GFF823 0.54 -0.3 812,419 + GFF823 0.54 +0.2 812,419 + GFF823 0.54 -2.6 812,419 + GFF823 0.54 -1.4 812,419 + GFF823 0.54 -0.1 812,419 + GFF823 0.54 -0.9 812,427 - GFF823 0.56 +0.2 812,427 - GFF823 0.56 -2.3 812,427 - GFF823 0.56 -1.4 812,427 - GFF823 0.56 +0.5 812,427 - GFF823 0.56 -1.6 812,427 - GFF823 0.56 -2.6 812,427 - GFF823 0.56 -2.1 812,427 - GFF823 0.56 -0.6 812,427 - GFF823 0.56 -1.4 812,554 + GFF823 0.81 -1.1 812,571 + GFF823 0.84 -2.4 812,639 + +0.2 812,659 + +0.6 812,691 + +1.1 812,691 + +0.9 812,696 + -0.5 812,708 + -2.3 812,710 + -1.1 812,710 + +0.2 812,710 + -0.3 812,716 - -2.1 812,716 - -0.8 812,752 + GFF824 0.11 -0.1 812,782 + GFF824 0.14 -1.2 812,805 - GFF824 0.16 -2.1 812,805 - GFF824 0.16 -0.9 812,805 - GFF824 0.16 -0.8 812,838 - GFF824 0.20 -1.6 812,838 - GFF824 0.20 -0.5 812,838 - GFF824 0.20 -3.1 812,838 - GFF824 0.20 -2.9 812,878 + GFF824 0.24 -0.5 812,886 - GFF824 0.25 -1.7 812,886 - GFF824 0.25 -0.6 812,975 - GFF824 0.34 +0.2 812,985 - GFF824 0.35 -1.6 813,094 + GFF824 0.47 -0.3 813,319 - GFF824 0.70 +0.4 813,360 - GFF824 0.75 -0.3 813,368 + GFF824 0.75 -1.5 813,559 - -2.4 813,619 + -1.7 813,624 + +0.5 813,624 + -2.3 813,632 - -0.0 813,632 - -0.6 813,632 - -1.4
Or see this region's nucleotide sequence