Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF820 and GFF821 are separated by 64 nucleotides GFF821 and GFF822 overlap by 4 nucleotides GFF822 and GFF823 are separated by 28 nucleotides GFF823 and GFF824 overlap by 4 nucleotides
GFF820 - hypothetical protein, at 809,666 to 810,544
GFF820
GFF821 - 'Pyridoxal-5'-phosphate phosphatase (EC 3.1.3.74), Alphaproteobacterial type' transl_table=11, at 810,609 to 811,280
GFF821
GFF822 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117), at 811,277 to 812,116
GFF822
GFF823 - Adenosylcobinamide kinase (EC 2.7.1.156) / Adenosylcobinamide-phosphate guanylyltransferase (EC 2.7.7.62), at 812,145 to 812,651
GFF823
GFF824 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 812,648 to 813,601
GFF824
Position (kb) 811
812
813 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 810.305 kb on - strand, within GFF820 at 810.333 kb on + strand, within GFF820 at 810.337 kb on + strand, within GFF820 at 810.341 kb on - strand, within GFF820 at 810.341 kb on - strand, within GFF820 at 810.458 kb on + strand at 810.463 kb on + strand at 810.466 kb on - strand at 810.487 kb on + strand at 810.500 kb on + strand at 810.500 kb on + strand at 810.500 kb on + strand at 810.534 kb on + strand at 810.535 kb on - strand at 810.555 kb on + strand at 810.564 kb on - strand at 810.571 kb on + strand at 810.576 kb on + strand at 810.576 kb on + strand at 810.579 kb on - strand at 810.579 kb on - strand at 810.579 kb on - strand at 810.579 kb on - strand at 810.601 kb on - strand at 810.618 kb on + strand at 810.618 kb on + strand at 810.618 kb on + strand at 810.618 kb on + strand at 810.620 kb on + strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.626 kb on - strand at 810.627 kb on - strand at 810.648 kb on + strand at 810.648 kb on + strand at 810.653 kb on + strand at 810.653 kb on + strand at 810.653 kb on + strand at 810.653 kb on + strand at 810.656 kb on - strand at 810.661 kb on - strand at 810.670 kb on - strand at 810.679 kb on - strand, within GFF821 at 810.685 kb on + strand, within GFF821 at 810.694 kb on - strand, within GFF821 at 810.704 kb on + strand, within GFF821 at 810.715 kb on + strand, within GFF821 at 810.715 kb on + strand, within GFF821 at 810.727 kb on - strand, within GFF821 at 810.729 kb on + strand, within GFF821 at 810.736 kb on + strand, within GFF821 at 810.737 kb on - strand, within GFF821 at 810.737 kb on - strand, within GFF821 at 810.785 kb on + strand, within GFF821 at 810.795 kb on + strand, within GFF821 at 810.795 kb on + strand, within GFF821 at 810.795 kb on + strand, within GFF821 at 810.797 kb on - strand, within GFF821 at 810.798 kb on - strand, within GFF821 at 810.803 kb on - strand, within GFF821 at 810.823 kb on - strand, within GFF821 at 810.852 kb on + strand, within GFF821 at 810.852 kb on + strand, within GFF821 at 810.852 kb on + strand, within GFF821 at 810.858 kb on - strand, within GFF821 at 810.860 kb on + strand, within GFF821 at 810.860 kb on + strand, within GFF821 at 810.868 kb on - strand, within GFF821 at 810.878 kb on + strand, within GFF821 at 810.891 kb on + strand, within GFF821 at 810.899 kb on - strand, within GFF821 at 810.907 kb on + strand, within GFF821 at 810.912 kb on + strand, within GFF821 at 810.912 kb on + strand, within GFF821 at 810.915 kb on - strand, within GFF821 at 810.915 kb on - strand, within GFF821 at 810.922 kb on + strand, within GFF821 at 810.927 kb on + strand, within GFF821 at 810.940 kb on - strand, within GFF821 at 810.941 kb on - strand, within GFF821 at 810.956 kb on - strand, within GFF821 at 810.997 kb on + strand, within GFF821 at 810.997 kb on + strand, within GFF821 at 811.005 kb on - strand, within GFF821 at 811.017 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.037 kb on + strand, within GFF821 at 811.045 kb on - strand, within GFF821 at 811.045 kb on - strand, within GFF821 at 811.045 kb on - strand, within GFF821 at 811.050 kb on - strand, within GFF821 at 811.055 kb on + strand, within GFF821 at 811.058 kb on - strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.060 kb on + strand, within GFF821 at 811.063 kb on - strand, within GFF821 at 811.065 kb on + strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.068 kb on - strand, within GFF821 at 811.070 kb on + strand, within GFF821 at 811.070 kb on + strand, within GFF821 at 811.075 kb on - strand, within GFF821 at 811.075 kb on - strand, within GFF821 at 811.078 kb on - strand, within GFF821 at 811.078 kb on - strand, within GFF821 at 811.078 kb on - strand, within GFF821 at 811.078 kb on - strand, within GFF821 at 811.078 kb on - strand, within GFF821 at 811.084 kb on + strand, within GFF821 at 811.084 kb on + strand, within GFF821 at 811.084 kb on + strand, within GFF821 at 811.084 kb on + strand, within GFF821 at 811.089 kb on + strand, within GFF821 at 811.089 kb on + strand, within GFF821 at 811.092 kb on - strand, within GFF821 at 811.092 kb on - strand, within GFF821 at 811.092 kb on - strand, within GFF821 at 811.092 kb on - strand, within GFF821 at 811.092 kb on - strand, within GFF821 at 811.129 kb on + strand, within GFF821 at 811.130 kb on + strand, within GFF821 at 811.138 kb on - strand, within GFF821 at 811.138 kb on - strand, within GFF821 at 811.138 kb on - strand, within GFF821 at 811.166 kb on - strand, within GFF821 at 811.180 kb on + strand, within GFF821 at 811.180 kb on + strand, within GFF821 at 811.180 kb on + strand, within GFF821 at 811.180 kb on + strand, within GFF821 at 811.300 kb on + strand at 812.120 kb on - strand at 812.120 kb on - strand at 812.135 kb on - strand at 812.142 kb on + strand at 812.142 kb on + strand at 812.142 kb on + strand at 812.148 kb on + strand at 812.151 kb on + strand at 812.158 kb on - strand at 812.224 kb on + strand, within GFF823 at 812.224 kb on - strand, within GFF823 at 812.232 kb on - strand, within GFF823 at 812.282 kb on - strand, within GFF823 at 812.301 kb on + strand, within GFF823 at 812.369 kb on - strand, within GFF823 at 812.396 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.419 kb on + strand, within GFF823 at 812.422 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.427 kb on - strand, within GFF823 at 812.480 kb on + strand, within GFF823 at 812.497 kb on + strand, within GFF823 at 812.502 kb on + strand, within GFF823 at 812.509 kb on - strand, within GFF823 at 812.554 kb on + strand, within GFF823 at 812.562 kb on - strand, within GFF823 at 812.571 kb on + strand, within GFF823 at 812.602 kb on - strand at 812.633 kb on - strand at 812.651 kb on + strand at 812.652 kb on + strand at 812.659 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.691 kb on + strand at 812.694 kb on - strand at 812.696 kb on + strand at 812.704 kb on - strand at 812.708 kb on + strand at 812.708 kb on + strand at 812.710 kb on + strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.716 kb on - strand at 812.754 kb on + strand, within GFF824 at 812.782 kb on + strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.805 kb on - strand, within GFF824 at 812.824 kb on + strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.838 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.886 kb on - strand, within GFF824 at 812.922 kb on + strand, within GFF824 at 812.936 kb on + strand, within GFF824 at 812.936 kb on + strand, within GFF824 at 812.977 kb on + strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 812.985 kb on - strand, within GFF824 at 813.036 kb on + strand, within GFF824 at 813.061 kb on - strand, within GFF824 at 813.076 kb on - strand, within GFF824 at 813.085 kb on + strand, within GFF824 at 813.110 kb on + strand, within GFF824
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 810,305 - GFF820 0.73 +0.3 810,333 + GFF820 0.76 -0.3 810,337 + GFF820 0.76 -0.5 810,341 - GFF820 0.77 +0.3 810,341 - GFF820 0.77 -1.4 810,458 + -0.1 810,463 + +0.5 810,466 - -0.2 810,487 + -1.9 810,500 + +1.0 810,500 + -0.5 810,500 + +0.1 810,534 + -1.4 810,535 - -2.1 810,555 + -0.6 810,564 - +0.0 810,571 + -1.3 810,576 + -0.4 810,576 + -1.3 810,579 - -1.2 810,579 - +0.3 810,579 - -0.5 810,579 - +1.0 810,601 - +0.1 810,618 + -2.5 810,618 + -0.6 810,618 + -1.2 810,618 + -0.3 810,620 + -1.8 810,626 - -0.6 810,626 - -1.1 810,626 - -1.9 810,626 - -2.2 810,626 - -1.4 810,626 - -1.1 810,626 - -0.5 810,626 - -1.7 810,627 - -3.1 810,648 + -0.9 810,648 + -1.5 810,653 + -0.5 810,653 + -0.1 810,653 + -0.4 810,653 + -1.2 810,656 - -1.1 810,661 - -2.2 810,670 - +0.1 810,679 - GFF821 0.10 -0.4 810,685 + GFF821 0.11 -1.1 810,694 - GFF821 0.13 -0.1 810,704 + GFF821 0.14 +0.4 810,715 + GFF821 0.16 -0.1 810,715 + GFF821 0.16 -1.7 810,727 - GFF821 0.18 -1.1 810,729 + GFF821 0.18 -1.6 810,736 + GFF821 0.19 -2.3 810,737 - GFF821 0.19 -0.1 810,737 - GFF821 0.19 -1.2 810,785 + GFF821 0.26 -0.2 810,795 + GFF821 0.28 -0.3 810,795 + GFF821 0.28 -0.2 810,795 + GFF821 0.28 +0.5 810,797 - GFF821 0.28 +0.5 810,798 - GFF821 0.28 -2.3 810,803 - GFF821 0.29 -1.9 810,823 - GFF821 0.32 -1.6 810,852 + GFF821 0.36 -1.0 810,852 + GFF821 0.36 +0.3 810,852 + GFF821 0.36 +0.3 810,858 - GFF821 0.37 -2.4 810,860 + GFF821 0.37 -1.2 810,860 + GFF821 0.37 -1.4 810,868 - GFF821 0.39 -0.6 810,878 + GFF821 0.40 -1.8 810,891 + GFF821 0.42 -0.1 810,899 - GFF821 0.43 -1.5 810,907 + GFF821 0.44 +0.4 810,912 + GFF821 0.45 -0.3 810,912 + GFF821 0.45 -1.3 810,915 - GFF821 0.46 -3.5 810,915 - GFF821 0.46 -2.7 810,922 + GFF821 0.47 -1.4 810,927 + GFF821 0.47 -0.1 810,940 - GFF821 0.49 -1.5 810,941 - GFF821 0.49 -1.8 810,956 - GFF821 0.52 -3.1 810,997 + GFF821 0.58 +0.8 810,997 + GFF821 0.58 -0.2 811,005 - GFF821 0.59 -1.7 811,017 + GFF821 0.61 +0.7 811,037 + GFF821 0.64 -1.6 811,037 + GFF821 0.64 -1.0 811,037 + GFF821 0.64 -0.5 811,037 + GFF821 0.64 +0.5 811,037 + GFF821 0.64 +1.4 811,037 + GFF821 0.64 -0.7 811,037 + GFF821 0.64 +1.4 811,037 + GFF821 0.64 -0.6 811,045 - GFF821 0.65 -1.4 811,045 - GFF821 0.65 -1.3 811,045 - GFF821 0.65 -2.0 811,050 - GFF821 0.66 -1.3 811,055 + GFF821 0.66 +0.7 811,058 - GFF821 0.67 -2.8 811,060 + GFF821 0.67 -0.5 811,060 + GFF821 0.67 +0.7 811,060 + GFF821 0.67 -1.4 811,060 + GFF821 0.67 +0.0 811,060 + GFF821 0.67 -0.4 811,060 + GFF821 0.67 -0.5 811,063 - GFF821 0.68 -2.8 811,065 + GFF821 0.68 -1.0 811,068 - GFF821 0.68 -0.0 811,068 - GFF821 0.68 -1.7 811,068 - GFF821 0.68 -0.7 811,068 - GFF821 0.68 -1.9 811,068 - GFF821 0.68 -1.8 811,068 - GFF821 0.68 -1.1 811,068 - GFF821 0.68 -1.5 811,068 - GFF821 0.68 -1.3 811,068 - GFF821 0.68 -2.8 811,070 + GFF821 0.69 +0.3 811,070 + GFF821 0.69 -1.7 811,075 - GFF821 0.69 -1.5 811,075 - GFF821 0.69 -1.4 811,078 - GFF821 0.70 -2.3 811,078 - GFF821 0.70 -2.0 811,078 - GFF821 0.70 +0.1 811,078 - GFF821 0.70 -1.1 811,078 - GFF821 0.70 -0.4 811,084 + GFF821 0.71 -1.1 811,084 + GFF821 0.71 -0.2 811,084 + GFF821 0.71 +0.5 811,084 + GFF821 0.71 -2.0 811,089 + GFF821 0.71 -1.3 811,089 + GFF821 0.71 -0.3 811,092 - GFF821 0.72 -2.7 811,092 - GFF821 0.72 -1.5 811,092 - GFF821 0.72 -2.8 811,092 - GFF821 0.72 -1.6 811,092 - GFF821 0.72 -0.5 811,129 + GFF821 0.77 -0.1 811,130 + GFF821 0.78 -1.5 811,138 - GFF821 0.79 -1.7 811,138 - GFF821 0.79 -1.2 811,138 - GFF821 0.79 -1.3 811,166 - GFF821 0.83 -2.0 811,180 + GFF821 0.85 +1.1 811,180 + GFF821 0.85 +0.7 811,180 + GFF821 0.85 -1.2 811,180 + GFF821 0.85 +0.4 811,300 + -1.4 812,120 - +0.0 812,120 - +1.0 812,135 - -1.4 812,142 + -1.5 812,142 + +0.8 812,142 + -0.9 812,148 + -3.0 812,151 + -1.8 812,158 - +0.1 812,224 + GFF823 0.16 -1.4 812,224 - GFF823 0.16 -1.3 812,232 - GFF823 0.17 -1.8 812,282 - GFF823 0.27 -2.1 812,301 + GFF823 0.31 -2.5 812,369 - GFF823 0.44 -1.8 812,396 + GFF823 0.50 -1.3 812,419 + GFF823 0.54 -0.4 812,419 + GFF823 0.54 -2.5 812,419 + GFF823 0.54 -1.7 812,419 + GFF823 0.54 -1.5 812,419 + GFF823 0.54 -2.8 812,419 + GFF823 0.54 -1.5 812,419 + GFF823 0.54 -1.1 812,422 - GFF823 0.55 -2.2 812,427 - GFF823 0.56 -1.1 812,427 - GFF823 0.56 -1.5 812,427 - GFF823 0.56 -1.2 812,427 - GFF823 0.56 -2.5 812,427 - GFF823 0.56 -1.5 812,427 - GFF823 0.56 -2.7 812,427 - GFF823 0.56 -1.8 812,427 - GFF823 0.56 -2.8 812,427 - GFF823 0.56 -2.3 812,427 - GFF823 0.56 -2.1 812,427 - GFF823 0.56 -1.6 812,480 + GFF823 0.66 -1.4 812,497 + GFF823 0.69 -1.2 812,502 + GFF823 0.70 -1.3 812,509 - GFF823 0.72 -1.6 812,554 + GFF823 0.81 -1.2 812,562 - GFF823 0.82 -0.4 812,571 + GFF823 0.84 -2.5 812,602 - -2.3 812,633 - -2.8 812,651 + -0.5 812,652 + -2.3 812,659 + +0.5 812,691 + -2.2 812,691 + -1.8 812,691 + +0.8 812,694 - +1.0 812,696 + -0.6 812,704 - -1.4 812,708 + -2.4 812,708 + -1.3 812,710 + -1.2 812,716 - -1.0 812,716 - -2.2 812,716 - -1.0 812,754 + GFF824 0.11 -1.8 812,782 + GFF824 0.14 -1.3 812,805 - GFF824 0.16 -2.2 812,805 - GFF824 0.16 -1.1 812,805 - GFF824 0.16 -1.0 812,824 + GFF824 0.18 -1.6 812,838 - GFF824 0.20 -1.8 812,838 - GFF824 0.20 -3.2 812,886 - GFF824 0.25 -1.9 812,886 - GFF824 0.25 -1.3 812,922 + GFF824 0.29 -1.6 812,936 + GFF824 0.30 -1.4 812,936 + GFF824 0.30 -1.5 812,977 + GFF824 0.34 -1.6 812,985 - GFF824 0.35 -1.6 812,985 - GFF824 0.35 -1.7 813,036 + GFF824 0.41 -1.8 813,061 - GFF824 0.43 -2.5 813,076 - GFF824 0.45 -1.4 813,085 + GFF824 0.46 -2.5 813,110 + GFF824 0.48 -1.3
Or see this region's nucleotide sequence