Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF48 and GFF49 are separated by 69 nucleotides GFF49 and GFF50 are separated by 66 nucleotides GFF50 and GFF51 are separated by 50 nucleotides GFF51 and GFF52 are separated by 28 nucleotides
GFF48 - GTP-binding and nucleic acid-binding protein YchF, at 38,177 to 39,277
GFF48
GFF49 - hypothetical protein, at 39,347 to 40,057
GFF49
GFF50 - Phosphoglucomutase (EC 5.4.2.2), at 40,124 to 41,752
GFF50
GFF51 - hypothetical protein, at 41,803 to 41,949
GFF51
GFF52 - Choloylglycine hydrolase (EC 3.5.1.24), at 41,978 to 43,033
GFF52
Position (kb)
40
41
42 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 39.216 kb on + strand at 39.226 kb on + strand at 39.334 kb on + strand at 39.362 kb on - strand at 39.399 kb on + strand at 39.407 kb on - strand at 39.444 kb on - strand, within GFF49 at 39.536 kb on + strand, within GFF49 at 39.549 kb on + strand, within GFF49 at 39.582 kb on + strand, within GFF49 at 39.590 kb on - strand, within GFF49 at 39.608 kb on - strand, within GFF49 at 39.629 kb on - strand, within GFF49 at 39.653 kb on + strand, within GFF49 at 39.661 kb on - strand, within GFF49 at 39.691 kb on + strand, within GFF49 at 39.699 kb on - strand, within GFF49 at 39.721 kb on + strand, within GFF49 at 39.721 kb on + strand, within GFF49 at 39.729 kb on - strand, within GFF49 at 39.731 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.787 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.794 kb on - strand, within GFF49 at 39.804 kb on - strand, within GFF49 at 39.831 kb on + strand, within GFF49 at 39.836 kb on + strand, within GFF49 at 39.836 kb on + strand, within GFF49 at 39.839 kb on - strand, within GFF49 at 39.842 kb on + strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.845 kb on - strand, within GFF49 at 39.856 kb on - strand, within GFF49 at 39.894 kb on + strand, within GFF49 at 39.914 kb on + strand, within GFF49 at 39.939 kb on + strand, within GFF49 at 39.947 kb on - strand, within GFF49 at 39.978 kb on + strand, within GFF49 at 39.998 kb on + strand at 39.998 kb on + strand at 40.006 kb on - strand at 40.006 kb on - strand at 40.006 kb on - strand at 40.065 kb on + strand at 40.073 kb on - strand at 40.091 kb on - strand at 40.102 kb on + strand at 40.130 kb on - strand at 40.135 kb on - strand at 40.135 kb on - strand at 40.139 kb on + strand at 40.142 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.152 kb on - strand at 40.268 kb on - strand at 40.386 kb on - strand, within GFF50 at 40.386 kb on - strand, within GFF50 at 40.489 kb on - strand, within GFF50 at 40.661 kb on - strand, within GFF50 at 41.207 kb on - strand, within GFF50 at 41.418 kb on - strand, within GFF50 at 41.608 kb on - strand at 41.742 kb on + strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.752 kb on + strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.765 kb on - strand at 41.772 kb on - strand at 41.795 kb on - strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.808 kb on + strand at 41.813 kb on + strand at 41.816 kb on - strand at 41.816 kb on - strand at 41.883 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.904 kb on + strand, within GFF51 at 41.944 kb on + strand at 41.950 kb on + strand at 41.950 kb on + strand at 41.958 kb on - strand at 41.981 kb on - strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.024 kb on - strand at 42.031 kb on - strand at 42.102 kb on + strand, within GFF52 at 42.136 kb on + strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.176 kb on - strand, within GFF52 at 42.180 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.335 kb on + strand, within GFF52 at 42.396 kb on - strand, within GFF52 at 42.475 kb on - strand, within GFF52 at 42.483 kb on + strand, within GFF52 at 42.494 kb on + strand, within GFF52 at 42.558 kb on + strand, within GFF52 at 42.609 kb on - strand, within GFF52 at 42.655 kb on + strand, within GFF52 at 42.662 kb on + strand, within GFF52 at 42.692 kb on + strand, within GFF52 at 42.692 kb on - strand, within GFF52 at 42.709 kb on + strand, within GFF52 at 42.711 kb on + strand, within GFF52 at 42.716 kb on + strand, within GFF52 at 42.724 kb on - strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.736 kb on - strand, within GFF52 at 42.737 kb on + strand, within GFF52 at 42.741 kb on - strand, within GFF52
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 39,216 + -0.9 39,226 + +0.6 39,334 + -2.1 39,362 - -0.5 39,399 + +0.3 39,407 - +0.5 39,444 - GFF49 0.14 -0.1 39,536 + GFF49 0.27 +1.0 39,549 + GFF49 0.28 +0.4 39,582 + GFF49 0.33 -1.9 39,590 - GFF49 0.34 -0.4 39,608 - GFF49 0.37 +1.5 39,629 - GFF49 0.40 -1.4 39,653 + GFF49 0.43 +1.5 39,661 - GFF49 0.44 -0.4 39,691 + GFF49 0.48 -1.5 39,699 - GFF49 0.50 -0.5 39,721 + GFF49 0.53 -2.0 39,721 + GFF49 0.53 -0.3 39,729 - GFF49 0.54 -1.8 39,731 + GFF49 0.54 -1.8 39,780 + GFF49 0.61 -0.8 39,780 + GFF49 0.61 +0.4 39,780 + GFF49 0.61 +0.1 39,780 + GFF49 0.61 +1.6 39,787 - GFF49 0.62 +1.1 39,788 - GFF49 0.62 +2.0 39,788 - GFF49 0.62 -1.2 39,788 - GFF49 0.62 +0.4 39,794 - GFF49 0.63 +1.6 39,804 - GFF49 0.64 -1.3 39,831 + GFF49 0.68 -1.1 39,836 + GFF49 0.69 -0.2 39,836 + GFF49 0.69 +1.1 39,839 - GFF49 0.69 +1.2 39,842 + GFF49 0.70 +0.6 39,844 - GFF49 0.70 -2.7 39,844 - GFF49 0.70 -2.8 39,844 - GFF49 0.70 -0.6 39,844 - GFF49 0.70 -0.4 39,845 - GFF49 0.70 +0.6 39,856 - GFF49 0.72 +0.9 39,894 + GFF49 0.77 -0.2 39,914 + GFF49 0.80 +0.2 39,939 + GFF49 0.83 -0.0 39,947 - GFF49 0.84 -0.1 39,978 + GFF49 0.89 +1.3 39,998 + +0.0 39,998 + -0.1 40,006 - -1.3 40,006 - -0.9 40,006 - -0.5 40,065 + -1.1 40,073 - +0.3 40,091 - -0.1 40,102 + -1.0 40,130 - +2.1 40,135 - -2.5 40,135 - -1.1 40,139 + +0.6 40,142 - +0.0 40,147 - -0.3 40,147 - -0.7 40,147 - +0.7 40,147 - -0.3 40,147 - -1.1 40,147 - -0.4 40,152 - -1.5 40,268 - +0.6 40,386 - GFF50 0.16 -1.0 40,386 - GFF50 0.16 -0.0 40,489 - GFF50 0.22 +0.2 40,661 - GFF50 0.33 +1.0 41,207 - GFF50 0.66 +1.6 41,418 - GFF50 0.79 +0.6 41,608 - +0.6 41,742 + +0.6 41,750 - +0.2 41,750 - -2.7 41,750 - -0.1 41,750 - -1.4 41,752 + +0.4 41,760 - -0.3 41,760 - -1.4 41,760 - +1.0 41,760 - +0.6 41,760 - -0.2 41,760 - +0.3 41,765 - +1.0 41,772 - +0.4 41,795 - +0.2 41,803 + -0.1 41,803 + +0.7 41,803 + -0.1 41,808 + -1.3 41,813 + -0.2 41,816 - +0.8 41,816 - +0.2 41,883 - GFF51 0.54 -0.8 41,894 - GFF51 0.62 +0.1 41,894 - GFF51 0.62 +1.0 41,904 + GFF51 0.69 +0.4 41,944 + -0.6 41,950 + -1.0 41,950 + -0.5 41,958 - -0.8 41,981 - -0.0 42,006 + +1.0 42,006 + -0.3 42,006 + +0.3 42,024 - -0.3 42,031 - -1.1 42,102 + GFF52 0.12 +1.0 42,136 + GFF52 0.15 +0.6 42,144 - GFF52 0.16 +0.6 42,144 - GFF52 0.16 -2.0 42,144 - GFF52 0.16 +0.6 42,156 + GFF52 0.17 +0.4 42,156 + GFF52 0.17 -0.2 42,176 - GFF52 0.19 -0.2 42,180 + GFF52 0.19 +0.0 42,264 + GFF52 0.27 +2.6 42,264 + GFF52 0.27 +0.9 42,264 + GFF52 0.27 -0.7 42,264 + GFF52 0.27 -0.8 42,264 + GFF52 0.27 +1.2 42,272 - GFF52 0.28 -0.6 42,272 - GFF52 0.28 +1.3 42,272 - GFF52 0.28 +1.2 42,335 + GFF52 0.34 -1.6 42,396 - GFF52 0.40 +0.0 42,475 - GFF52 0.47 -1.9 42,483 + GFF52 0.48 -2.2 42,494 + GFF52 0.49 +1.0 42,558 + GFF52 0.55 -1.1 42,609 - GFF52 0.60 -0.1 42,655 + GFF52 0.64 -1.0 42,662 + GFF52 0.65 +1.9 42,692 + GFF52 0.68 +0.5 42,692 - GFF52 0.68 -0.5 42,709 + GFF52 0.69 -0.7 42,711 + GFF52 0.69 +0.7 42,716 + GFF52 0.70 +0.2 42,724 - GFF52 0.71 -1.0 42,728 + GFF52 0.71 -0.4 42,728 + GFF52 0.71 +1.0 42,728 + GFF52 0.71 -1.4 42,728 + GFF52 0.71 -1.0 42,736 - GFF52 0.72 -2.6 42,737 + GFF52 0.72 +0.2 42,741 - GFF52 0.72 -0.7
Or see this region's nucleotide sequence