Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3456 and GFF3457 are separated by 181 nucleotides GFF3457 and GFF3458 are separated by 85 nucleotides GFF3458 and GFF3459 are separated by 120 nucleotides GFF3459 and GFF3460 are separated by 38 nucleotides
GFF3456 - pyridoxal-phosphate dependent enzyme family protein, at 3,496,472 to 3,497,470
GFF3456
GFF3457 - hypothetical protein, at 3,497,652 to 3,498,068
GFF3457
GFF3458 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 3,498,154 to 3,499,197
GFF3458
GFF3459 - DNA recombination and repair protein RecO, at 3,499,318 to 3,500,052
GFF3459
GFF3460 - Integral membrane protein TerC, at 3,500,091 to 3,501,071
GFF3460
Position (kb)
3498
3499
3500 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3497.182 kb on + strand, within GFF3456 at 3497.246 kb on + strand, within GFF3456 at 3497.254 kb on - strand, within GFF3456 at 3497.254 kb on - strand, within GFF3456 at 3497.311 kb on + strand, within GFF3456 at 3497.311 kb on + strand, within GFF3456 at 3497.311 kb on + strand, within GFF3456 at 3497.311 kb on + strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.319 kb on - strand, within GFF3456 at 3497.362 kb on - strand, within GFF3456 at 3497.450 kb on + strand at 3497.463 kb on - strand at 3497.494 kb on - strand at 3497.513 kb on - strand at 3497.529 kb on + strand at 3497.537 kb on - strand at 3497.556 kb on - strand at 3497.628 kb on + strand at 3497.649 kb on + strand at 3497.649 kb on + strand at 3497.654 kb on + strand at 3497.757 kb on + strand, within GFF3457 at 3497.807 kb on + strand, within GFF3457 at 3497.838 kb on + strand, within GFF3457 at 3497.893 kb on + strand, within GFF3457 at 3497.906 kb on - strand, within GFF3457 at 3497.922 kb on + strand, within GFF3457 at 3497.924 kb on + strand, within GFF3457 at 3497.924 kb on + strand, within GFF3457 at 3497.924 kb on + strand, within GFF3457 at 3497.930 kb on - strand, within GFF3457 at 3497.931 kb on + strand, within GFF3457 at 3497.932 kb on - strand, within GFF3457 at 3497.935 kb on + strand, within GFF3457 at 3497.940 kb on + strand, within GFF3457 at 3497.945 kb on - strand, within GFF3457 at 3497.953 kb on - strand, within GFF3457 at 3497.967 kb on + strand, within GFF3457 at 3497.983 kb on - strand, within GFF3457 at 3498.043 kb on - strand at 3498.045 kb on - strand at 3498.048 kb on - strand at 3498.054 kb on - strand at 3498.181 kb on + strand at 3498.183 kb on + strand at 3498.205 kb on + strand at 3498.223 kb on + strand at 3498.247 kb on - strand at 3498.262 kb on - strand, within GFF3458 at 3498.282 kb on + strand, within GFF3458 at 3498.282 kb on + strand, within GFF3458 at 3498.295 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.338 kb on + strand, within GFF3458 at 3498.340 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.346 kb on - strand, within GFF3458 at 3498.356 kb on - strand, within GFF3458 at 3498.380 kb on + strand, within GFF3458 at 3498.386 kb on + strand, within GFF3458 at 3498.389 kb on - strand, within GFF3458 at 3498.389 kb on - strand, within GFF3458 at 3498.391 kb on + strand, within GFF3458 at 3498.398 kb on - strand, within GFF3458 at 3498.399 kb on - strand, within GFF3458 at 3498.401 kb on + strand, within GFF3458 at 3498.411 kb on + strand, within GFF3458 at 3498.448 kb on + strand, within GFF3458 at 3498.456 kb on + strand, within GFF3458 at 3498.457 kb on - strand, within GFF3458 at 3498.459 kb on + strand, within GFF3458 at 3498.459 kb on + strand, within GFF3458 at 3498.460 kb on + strand, within GFF3458 at 3498.463 kb on + strand, within GFF3458 at 3498.464 kb on + strand, within GFF3458 at 3498.468 kb on + strand, within GFF3458 at 3498.468 kb on + strand, within GFF3458 at 3498.468 kb on + strand, within GFF3458 at 3498.500 kb on + strand, within GFF3458 at 3498.510 kb on + strand, within GFF3458 at 3498.513 kb on - strand, within GFF3458 at 3498.518 kb on - strand, within GFF3458 at 3498.523 kb on - strand, within GFF3458 at 3498.523 kb on - strand, within GFF3458 at 3498.525 kb on - strand, within GFF3458 at 3498.559 kb on + strand, within GFF3458 at 3498.559 kb on + strand, within GFF3458 at 3498.566 kb on - strand, within GFF3458 at 3498.603 kb on - strand, within GFF3458 at 3498.615 kb on + strand, within GFF3458 at 3498.631 kb on - strand, within GFF3458 at 3498.632 kb on + strand, within GFF3458 at 3498.640 kb on - strand, within GFF3458 at 3498.640 kb on - strand, within GFF3458 at 3498.650 kb on + strand, within GFF3458 at 3498.658 kb on - strand, within GFF3458 at 3498.658 kb on - strand, within GFF3458 at 3498.658 kb on - strand, within GFF3458 at 3498.658 kb on - strand, within GFF3458 at 3498.670 kb on + strand, within GFF3458 at 3498.678 kb on - strand, within GFF3458 at 3498.696 kb on + strand, within GFF3458 at 3498.734 kb on + strand, within GFF3458 at 3498.736 kb on + strand, within GFF3458 at 3498.736 kb on + strand, within GFF3458 at 3498.736 kb on + strand, within GFF3458 at 3498.744 kb on - strand, within GFF3458 at 3498.744 kb on - strand, within GFF3458 at 3498.744 kb on - strand, within GFF3458 at 3498.753 kb on + strand, within GFF3458 at 3498.753 kb on + strand, within GFF3458 at 3498.759 kb on + strand, within GFF3458 at 3498.767 kb on - strand, within GFF3458 at 3498.767 kb on - strand, within GFF3458 at 3498.778 kb on - strand, within GFF3458 at 3498.783 kb on + strand, within GFF3458 at 3498.843 kb on + strand, within GFF3458 at 3498.851 kb on - strand, within GFF3458 at 3498.873 kb on + strand, within GFF3458 at 3498.902 kb on + strand, within GFF3458 at 3498.908 kb on - strand, within GFF3458 at 3498.908 kb on - strand, within GFF3458 at 3498.908 kb on - strand, within GFF3458 at 3498.920 kb on + strand, within GFF3458 at 3498.928 kb on - strand, within GFF3458 at 3499.003 kb on - strand, within GFF3458 at 3499.005 kb on + strand, within GFF3458 at 3499.005 kb on + strand, within GFF3458 at 3499.013 kb on - strand, within GFF3458 at 3499.025 kb on + strand, within GFF3458 at 3499.030 kb on + strand, within GFF3458 at 3499.087 kb on - strand, within GFF3458 at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.095 kb on + strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.103 kb on - strand at 3499.136 kb on + strand at 3499.144 kb on - strand at 3499.180 kb on - strand at 3499.331 kb on + strand at 3499.392 kb on + strand, within GFF3459 at 3499.392 kb on + strand, within GFF3459 at 3499.392 kb on + strand, within GFF3459 at 3499.400 kb on - strand, within GFF3459 at 3499.409 kb on - strand, within GFF3459 at 3499.441 kb on - strand, within GFF3459 at 3499.458 kb on - strand, within GFF3459 at 3499.465 kb on + strand, within GFF3459 at 3499.484 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.502 kb on + strand, within GFF3459 at 3499.508 kb on - strand, within GFF3459 at 3499.517 kb on - strand, within GFF3459 at 3499.522 kb on - strand, within GFF3459 at 3499.566 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.594 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.599 kb on + strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.602 kb on - strand, within GFF3459 at 3499.607 kb on - strand, within GFF3459 at 3499.620 kb on - strand, within GFF3459 at 3499.653 kb on - strand, within GFF3459 at 3499.653 kb on - strand, within GFF3459 at 3499.658 kb on + strand, within GFF3459 at 3499.658 kb on + strand, within GFF3459 at 3499.664 kb on - strand, within GFF3459 at 3499.666 kb on - strand, within GFF3459 at 3499.739 kb on + strand, within GFF3459 at 3499.766 kb on + strand, within GFF3459 at 3499.822 kb on + strand, within GFF3459 at 3499.823 kb on - strand, within GFF3459 at 3499.827 kb on + strand, within GFF3459 at 3499.831 kb on - strand, within GFF3459 at 3499.840 kb on + strand, within GFF3459 at 3499.903 kb on + strand, within GFF3459 at 3499.918 kb on - strand, within GFF3459 at 3499.948 kb on + strand, within GFF3459 at 3499.956 kb on - strand, within GFF3459 at 3499.965 kb on + strand, within GFF3459 at 3499.967 kb on - strand, within GFF3459 at 3499.967 kb on - strand, within GFF3459 at 3499.970 kb on + strand, within GFF3459 at 3499.978 kb on - strand, within GFF3459 at 3500.026 kb on - strand at 3500.026 kb on - strand at 3500.028 kb on + strand at 3500.048 kb on + strand at 3500.056 kb on - strand at 3500.056 kb on - strand at 3500.062 kb on - strand at 3500.111 kb on + strand at 3500.111 kb on + strand at 3500.119 kb on - strand at 3500.119 kb on - strand at 3500.119 kb on - strand at 3500.124 kb on - strand at 3500.144 kb on - strand at 3500.144 kb on - strand at 3500.146 kb on - strand at 3500.151 kb on - strand at 3500.159 kb on - strand at 3500.161 kb on - strand at 3500.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 3,497,182 + GFF3456 0.71 +0.5 3,497,246 + GFF3456 0.77 -0.6 3,497,254 - GFF3456 0.78 -0.2 3,497,254 - GFF3456 0.78 +0.2 3,497,311 + GFF3456 0.84 -2.4 3,497,311 + GFF3456 0.84 -0.0 3,497,311 + GFF3456 0.84 -1.7 3,497,311 + GFF3456 0.84 -1.3 3,497,319 - GFF3456 0.85 +0.1 3,497,319 - GFF3456 0.85 +0.6 3,497,319 - GFF3456 0.85 -1.2 3,497,319 - GFF3456 0.85 +0.5 3,497,319 - GFF3456 0.85 -2.0 3,497,319 - GFF3456 0.85 -0.6 3,497,362 - GFF3456 0.89 -0.4 3,497,450 + -1.0 3,497,463 - +0.6 3,497,494 - -1.1 3,497,513 - +0.1 3,497,529 + +1.6 3,497,537 - +0.4 3,497,556 - +0.3 3,497,628 + -0.3 3,497,649 + +1.0 3,497,649 + -1.9 3,497,654 + -0.1 3,497,757 + GFF3457 0.25 +0.5 3,497,807 + GFF3457 0.37 -0.5 3,497,838 + GFF3457 0.45 +0.0 3,497,893 + GFF3457 0.58 -0.1 3,497,906 - GFF3457 0.61 -0.6 3,497,922 + GFF3457 0.65 -0.7 3,497,924 + GFF3457 0.65 -2.1 3,497,924 + GFF3457 0.65 -0.2 3,497,924 + GFF3457 0.65 -1.3 3,497,930 - GFF3457 0.67 +1.8 3,497,931 + GFF3457 0.67 +0.6 3,497,932 - GFF3457 0.67 -0.1 3,497,935 + GFF3457 0.68 -0.6 3,497,940 + GFF3457 0.69 -0.5 3,497,945 - GFF3457 0.70 -1.6 3,497,953 - GFF3457 0.72 +0.2 3,497,967 + GFF3457 0.76 -0.2 3,497,983 - GFF3457 0.79 +0.2 3,498,043 - +0.4 3,498,045 - +0.4 3,498,048 - -0.3 3,498,054 - -0.7 3,498,181 + -2.5 3,498,183 + +0.1 3,498,205 + -0.8 3,498,223 + -2.5 3,498,247 - -2.6 3,498,262 - GFF3458 0.10 -2.4 3,498,282 + GFF3458 0.12 -1.7 3,498,282 + GFF3458 0.12 -2.0 3,498,295 + GFF3458 0.14 -1.2 3,498,338 + GFF3458 0.18 -2.9 3,498,338 + GFF3458 0.18 -0.8 3,498,338 + GFF3458 0.18 -3.1 3,498,338 + GFF3458 0.18 -2.4 3,498,338 + GFF3458 0.18 -1.4 3,498,338 + GFF3458 0.18 -3.5 3,498,338 + GFF3458 0.18 -3.0 3,498,340 - GFF3458 0.18 -0.5 3,498,346 - GFF3458 0.18 -1.5 3,498,346 - GFF3458 0.18 -0.8 3,498,346 - GFF3458 0.18 -2.4 3,498,346 - GFF3458 0.18 -2.7 3,498,346 - GFF3458 0.18 -1.7 3,498,346 - GFF3458 0.18 -2.0 3,498,356 - GFF3458 0.19 -1.2 3,498,380 + GFF3458 0.22 -2.0 3,498,386 + GFF3458 0.22 -2.3 3,498,389 - GFF3458 0.23 -2.5 3,498,389 - GFF3458 0.23 -2.0 3,498,391 + GFF3458 0.23 -2.4 3,498,398 - GFF3458 0.23 -3.4 3,498,399 - GFF3458 0.23 -1.0 3,498,401 + GFF3458 0.24 -2.5 3,498,411 + GFF3458 0.25 -4.2 3,498,448 + GFF3458 0.28 -2.1 3,498,456 + GFF3458 0.29 -1.7 3,498,457 - GFF3458 0.29 -3.4 3,498,459 + GFF3458 0.29 -2.9 3,498,459 + GFF3458 0.29 -1.2 3,498,460 + GFF3458 0.29 -1.5 3,498,463 + GFF3458 0.30 -1.6 3,498,464 + GFF3458 0.30 -1.9 3,498,468 + GFF3458 0.30 -1.1 3,498,468 + GFF3458 0.30 -2.5 3,498,468 + GFF3458 0.30 -3.1 3,498,500 + GFF3458 0.33 -0.8 3,498,510 + GFF3458 0.34 -1.6 3,498,513 - GFF3458 0.34 -2.5 3,498,518 - GFF3458 0.35 -0.1 3,498,523 - GFF3458 0.35 -2.1 3,498,523 - GFF3458 0.35 -2.4 3,498,525 - GFF3458 0.36 -0.9 3,498,559 + GFF3458 0.39 -2.3 3,498,559 + GFF3458 0.39 -1.0 3,498,566 - GFF3458 0.39 -0.8 3,498,603 - GFF3458 0.43 -2.0 3,498,615 + GFF3458 0.44 -2.0 3,498,631 - GFF3458 0.46 -1.3 3,498,632 + GFF3458 0.46 -1.7 3,498,640 - GFF3458 0.47 -2.3 3,498,640 - GFF3458 0.47 -2.9 3,498,650 + GFF3458 0.48 -0.9 3,498,658 - GFF3458 0.48 -1.8 3,498,658 - GFF3458 0.48 -0.9 3,498,658 - GFF3458 0.48 -2.5 3,498,658 - GFF3458 0.48 -1.7 3,498,670 + GFF3458 0.49 -1.3 3,498,678 - GFF3458 0.50 -2.7 3,498,696 + GFF3458 0.52 -2.1 3,498,734 + GFF3458 0.56 -1.2 3,498,736 + GFF3458 0.56 -1.0 3,498,736 + GFF3458 0.56 -1.7 3,498,736 + GFF3458 0.56 -2.0 3,498,744 - GFF3458 0.57 -1.4 3,498,744 - GFF3458 0.57 -0.8 3,498,744 - GFF3458 0.57 -2.2 3,498,753 + GFF3458 0.57 -1.2 3,498,753 + GFF3458 0.57 -0.5 3,498,759 + GFF3458 0.58 -1.4 3,498,767 - GFF3458 0.59 -2.5 3,498,767 - GFF3458 0.59 -1.8 3,498,778 - GFF3458 0.60 -2.7 3,498,783 + GFF3458 0.60 -1.2 3,498,843 + GFF3458 0.66 -1.7 3,498,851 - GFF3458 0.67 -1.5 3,498,873 + GFF3458 0.69 -3.0 3,498,902 + GFF3458 0.72 -3.6 3,498,908 - GFF3458 0.72 -1.0 3,498,908 - GFF3458 0.72 -1.7 3,498,908 - GFF3458 0.72 -2.4 3,498,920 + GFF3458 0.73 -1.7 3,498,928 - GFF3458 0.74 -2.4 3,499,003 - GFF3458 0.81 -2.1 3,499,005 + GFF3458 0.82 -0.8 3,499,005 + GFF3458 0.82 -2.6 3,499,013 - GFF3458 0.82 -2.4 3,499,025 + GFF3458 0.83 -2.6 3,499,030 + GFF3458 0.84 -2.2 3,499,087 - GFF3458 0.89 -2.5 3,499,095 + -1.4 3,499,095 + -2.4 3,499,095 + -3.1 3,499,095 + -2.3 3,499,103 - -1.4 3,499,103 - -2.3 3,499,103 - -2.4 3,499,103 - -2.6 3,499,136 + -2.0 3,499,144 - -2.0 3,499,180 - -1.7 3,499,331 + -1.7 3,499,392 + GFF3459 0.10 +1.5 3,499,392 + GFF3459 0.10 +0.7 3,499,392 + GFF3459 0.10 +0.6 3,499,400 - GFF3459 0.11 +1.1 3,499,409 - GFF3459 0.12 -1.3 3,499,441 - GFF3459 0.17 -1.3 3,499,458 - GFF3459 0.19 -0.4 3,499,465 + GFF3459 0.20 +1.1 3,499,484 + GFF3459 0.23 +0.0 3,499,502 + GFF3459 0.25 +0.3 3,499,502 + GFF3459 0.25 -1.0 3,499,502 + GFF3459 0.25 +1.3 3,499,508 - GFF3459 0.26 +0.2 3,499,517 - GFF3459 0.27 +0.2 3,499,522 - GFF3459 0.28 +1.2 3,499,566 + GFF3459 0.34 +1.0 3,499,594 + GFF3459 0.38 +0.2 3,499,594 + GFF3459 0.38 -0.3 3,499,594 + GFF3459 0.38 -0.4 3,499,599 + GFF3459 0.38 +0.5 3,499,599 + GFF3459 0.38 -2.1 3,499,599 + GFF3459 0.38 +0.1 3,499,602 - GFF3459 0.39 +1.1 3,499,602 - GFF3459 0.39 -0.2 3,499,602 - GFF3459 0.39 -0.9 3,499,602 - GFF3459 0.39 -0.8 3,499,607 - GFF3459 0.39 -0.9 3,499,620 - GFF3459 0.41 -0.1 3,499,653 - GFF3459 0.46 +2.0 3,499,653 - GFF3459 0.46 -0.2 3,499,658 + GFF3459 0.46 -1.6 3,499,658 + GFF3459 0.46 -0.7 3,499,664 - GFF3459 0.47 -0.8 3,499,666 - GFF3459 0.47 +2.4 3,499,739 + GFF3459 0.57 -0.7 3,499,766 + GFF3459 0.61 -0.1 3,499,822 + GFF3459 0.69 -0.1 3,499,823 - GFF3459 0.69 -0.9 3,499,827 + GFF3459 0.69 +0.5 3,499,831 - GFF3459 0.70 +0.1 3,499,840 + GFF3459 0.71 -1.1 3,499,903 + GFF3459 0.80 -1.2 3,499,918 - GFF3459 0.82 +1.7 3,499,948 + GFF3459 0.86 +0.4 3,499,956 - GFF3459 0.87 +0.8 3,499,965 + GFF3459 0.88 -1.2 3,499,967 - GFF3459 0.88 -1.0 3,499,967 - GFF3459 0.88 +0.0 3,499,970 + GFF3459 0.89 -0.6 3,499,978 - GFF3459 0.90 +0.2 3,500,026 - -0.2 3,500,026 - +0.2 3,500,028 + -1.9 3,500,048 + -0.0 3,500,056 - -1.7 3,500,056 - -1.8 3,500,062 - -0.5 3,500,111 + +0.7 3,500,111 + -0.3 3,500,119 - -1.3 3,500,119 - +0.3 3,500,119 - +1.0 3,500,124 - -0.5 3,500,144 - -0.2 3,500,144 - -1.8 3,500,146 - +0.7 3,500,151 - -0.8 3,500,159 - +0.8 3,500,161 - -0.4 3,500,164 - +0.2
Or see this region's nucleotide sequence