Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3227 and GFF3228 overlap by 4 nucleotides GFF3228 and GFF3229 are separated by 27 nucleotides GFF3229 and GFF3230 are separated by 568 nucleotides GFF3230 and GFF3231 are separated by 47 nucleotides
GFF3227 - hypothetical protein, at 3,282,530 to 3,283,864
GFF3227
GFF3228 - hypothetical protein, at 3,283,861 to 3,284,727
GFF3228
GFF3229 - hypothetical protein, at 3,284,755 to 3,285,036
GFF3229
GFF3230 - Transposase, at 3,285,605 to 3,285,832
GFF3230
GFF3231 - Transposase, at 3,285,880 to 3,286,704
GFF3231
Position (kb)
3284
3285
3286 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3283.828 kb on + strand at 3283.841 kb on - strand at 3283.841 kb on - strand at 3283.843 kb on + strand at 3283.858 kb on + strand at 3283.859 kb on + strand at 3283.861 kb on - strand at 3283.866 kb on + strand at 3283.866 kb on + strand at 3283.866 kb on + strand at 3283.866 kb on + strand at 3283.866 kb on + strand at 3283.866 kb on - strand at 3283.873 kb on - strand at 3283.874 kb on - strand at 3283.874 kb on - strand at 3283.874 kb on - strand at 3283.874 kb on - strand at 3283.896 kb on + strand at 3283.903 kb on + strand at 3283.904 kb on - strand at 3283.916 kb on + strand at 3283.929 kb on + strand at 3283.930 kb on - strand at 3283.937 kb on - strand at 3283.948 kb on - strand, within GFF3228 at 3283.950 kb on + strand, within GFF3228 at 3283.950 kb on + strand, within GFF3228 at 3283.958 kb on + strand, within GFF3228 at 3283.960 kb on + strand, within GFF3228 at 3283.961 kb on + strand, within GFF3228 at 3284.007 kb on - strand, within GFF3228 at 3284.089 kb on + strand, within GFF3228 at 3284.120 kb on + strand, within GFF3228 at 3284.220 kb on + strand, within GFF3228 at 3284.222 kb on + strand, within GFF3228 at 3284.232 kb on + strand, within GFF3228 at 3284.239 kb on + strand, within GFF3228 at 3284.256 kb on + strand, within GFF3228 at 3284.261 kb on + strand, within GFF3228 at 3284.261 kb on + strand, within GFF3228 at 3284.264 kb on - strand, within GFF3228 at 3284.264 kb on - strand, within GFF3228 at 3284.269 kb on - strand, within GFF3228 at 3284.315 kb on + strand, within GFF3228 at 3284.323 kb on - strand, within GFF3228 at 3284.328 kb on - strand, within GFF3228 at 3284.353 kb on + strand, within GFF3228 at 3284.370 kb on + strand, within GFF3228 at 3284.438 kb on - strand, within GFF3228 at 3284.438 kb on - strand, within GFF3228 at 3284.443 kb on - strand, within GFF3228 at 3284.454 kb on + strand, within GFF3228 at 3284.454 kb on + strand, within GFF3228 at 3284.463 kb on + strand, within GFF3228 at 3284.463 kb on - strand, within GFF3228 at 3284.464 kb on - strand, within GFF3228 at 3284.475 kb on + strand, within GFF3228 at 3284.483 kb on + strand, within GFF3228 at 3284.511 kb on + strand, within GFF3228 at 3284.521 kb on - strand, within GFF3228 at 3284.581 kb on + strand, within GFF3228 at 3284.581 kb on + strand, within GFF3228 at 3284.594 kb on + strand, within GFF3228 at 3284.594 kb on + strand, within GFF3228 at 3284.594 kb on + strand, within GFF3228 at 3284.594 kb on + strand, within GFF3228 at 3284.602 kb on - strand, within GFF3228 at 3284.602 kb on - strand, within GFF3228 at 3284.602 kb on - strand, within GFF3228 at 3284.621 kb on - strand, within GFF3228 at 3284.621 kb on - strand, within GFF3228 at 3284.621 kb on - strand, within GFF3228 at 3284.683 kb on - strand at 3284.685 kb on + strand at 3284.687 kb on + strand at 3284.688 kb on - strand at 3284.692 kb on + strand at 3284.693 kb on - strand at 3284.693 kb on - strand at 3284.741 kb on - strand at 3284.758 kb on + strand at 3284.767 kb on - strand at 3284.810 kb on - strand, within GFF3229 at 3284.811 kb on + strand, within GFF3229 at 3284.825 kb on - strand, within GFF3229 at 3284.826 kb on + strand, within GFF3229 at 3284.826 kb on + strand, within GFF3229 at 3284.826 kb on + strand, within GFF3229 at 3284.826 kb on + strand, within GFF3229 at 3284.826 kb on + strand, within GFF3229 at 3284.834 kb on - strand, within GFF3229 at 3284.834 kb on - strand, within GFF3229 at 3284.834 kb on - strand, within GFF3229 at 3284.834 kb on - strand, within GFF3229 at 3284.886 kb on + strand, within GFF3229 at 3284.891 kb on + strand, within GFF3229 at 3284.891 kb on + strand, within GFF3229 at 3284.891 kb on + strand, within GFF3229 at 3284.894 kb on - strand, within GFF3229 at 3284.901 kb on + strand, within GFF3229 at 3284.901 kb on + strand, within GFF3229 at 3284.906 kb on + strand, within GFF3229 at 3284.909 kb on - strand, within GFF3229 at 3284.909 kb on - strand, within GFF3229 at 3284.909 kb on - strand, within GFF3229 at 3284.909 kb on - strand, within GFF3229 at 3284.926 kb on + strand, within GFF3229 at 3284.951 kb on + strand, within GFF3229 at 3284.964 kb on + strand, within GFF3229 at 3284.969 kb on + strand, within GFF3229 at 3284.977 kb on + strand, within GFF3229 at 3284.985 kb on - strand, within GFF3229 at 3284.993 kb on + strand, within GFF3229 at 3285.007 kb on - strand, within GFF3229 at 3285.007 kb on - strand, within GFF3229 at 3285.007 kb on - strand, within GFF3229 at 3285.013 kb on + strand at 3285.013 kb on + strand at 3285.013 kb on + strand at 3285.013 kb on + strand at 3285.021 kb on - strand at 3285.021 kb on - strand at 3285.021 kb on - strand at 3285.026 kb on - strand at 3285.036 kb on + strand at 3285.046 kb on + strand at 3285.046 kb on + strand at 3285.061 kb on - strand at 3285.061 kb on - strand at 3285.061 kb on - strand at 3285.061 kb on - strand at 3285.063 kb on + strand at 3285.071 kb on - strand at 3285.074 kb on + strand at 3285.087 kb on + strand at 3285.137 kb on + strand at 3285.177 kb on + strand at 3285.231 kb on + strand at 3285.294 kb on + strand at 3285.306 kb on + strand at 3285.306 kb on + strand at 3285.311 kb on + strand at 3285.311 kb on + strand at 3285.311 kb on - strand at 3285.314 kb on - strand at 3285.314 kb on - strand at 3285.350 kb on + strand at 3285.353 kb on + strand at 3285.353 kb on + strand at 3285.386 kb on + strand at 3285.396 kb on + strand at 3285.412 kb on + strand at 3285.412 kb on + strand at 3285.420 kb on - strand at 3285.420 kb on - strand at 3285.420 kb on - strand at 3285.427 kb on - strand at 3285.447 kb on + strand at 3285.461 kb on - strand at 3285.501 kb on + strand at 3285.510 kb on + strand at 3285.514 kb on + strand at 3285.518 kb on - strand at 3285.522 kb on - strand at 3285.536 kb on - strand at 3285.542 kb on + strand at 3285.545 kb on - strand at 3285.564 kb on - strand at 3285.566 kb on + strand at 3285.566 kb on + strand at 3285.566 kb on + strand at 3285.571 kb on + strand at 3285.574 kb on - strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.576 kb on + strand at 3285.579 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.584 kb on - strand at 3285.587 kb on + strand at 3285.602 kb on - strand at 3285.602 kb on - strand at 3285.616 kb on - strand at 3285.616 kb on - strand at 3285.628 kb on + strand, within GFF3230 at 3285.630 kb on + strand, within GFF3230 at 3285.636 kb on - strand, within GFF3230 at 3285.636 kb on - strand, within GFF3230 at 3285.636 kb on - strand, within GFF3230 at 3285.636 kb on - strand, within GFF3230 at 3285.642 kb on + strand, within GFF3230 at 3285.653 kb on - strand, within GFF3230 at 3285.655 kb on + strand, within GFF3230 at 3285.660 kb on + strand, within GFF3230 at 3285.668 kb on - strand, within GFF3230 at 3285.700 kb on + strand, within GFF3230 at 3285.700 kb on + strand, within GFF3230 at 3285.714 kb on - strand, within GFF3230 at 3285.716 kb on + strand, within GFF3230 at 3285.718 kb on - strand, within GFF3230 at 3285.732 kb on - strand, within GFF3230 at 3285.734 kb on + strand, within GFF3230 at 3285.756 kb on - strand, within GFF3230 at 3285.763 kb on + strand, within GFF3230 at 3285.763 kb on + strand, within GFF3230 at 3285.776 kb on - strand, within GFF3230 at 3285.779 kb on - strand, within GFF3230 at 3285.781 kb on - strand, within GFF3230 at 3285.844 kb on - strand at 3285.854 kb on + strand at 3285.862 kb on - strand at 3285.872 kb on - strand at 3285.888 kb on - strand at 3285.890 kb on - strand at 3285.892 kb on - strand at 3285.892 kb on - strand at 3285.903 kb on + strand at 3285.927 kb on + strand at 3285.939 kb on - strand at 3285.947 kb on - strand at 3285.948 kb on + strand at 3285.956 kb on - strand at 3285.961 kb on + strand at 3285.967 kb on - strand, within GFF3231 at 3285.973 kb on + strand, within GFF3231 at 3285.973 kb on + strand, within GFF3231 at 3285.981 kb on - strand, within GFF3231 at 3285.981 kb on - strand, within GFF3231 at 3285.981 kb on - strand, within GFF3231 at 3285.981 kb on - strand, within GFF3231 at 3285.988 kb on + strand, within GFF3231 at 3285.996 kb on - strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.008 kb on + strand, within GFF3231 at 3286.009 kb on + strand, within GFF3231 at 3286.009 kb on + strand, within GFF3231 at 3286.009 kb on + strand, within GFF3231 at 3286.009 kb on + strand, within GFF3231 at 3286.009 kb on + strand, within GFF3231 at 3286.016 kb on - strand, within GFF3231 at 3286.016 kb on - strand, within GFF3231 at 3286.016 kb on - strand, within GFF3231 at 3286.016 kb on - strand, within GFF3231 at 3286.016 kb on - strand, within GFF3231 at 3286.017 kb on - strand, within GFF3231 at 3286.017 kb on - strand, within GFF3231 at 3286.017 kb on - strand, within GFF3231 at 3286.017 kb on - strand, within GFF3231 at 3286.027 kb on - strand, within GFF3231 at 3286.035 kb on - strand, within GFF3231
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 3,283,828 + +0.4 3,283,841 - -1.4 3,283,841 - -0.3 3,283,843 + -0.7 3,283,858 + -2.3 3,283,859 + -1.8 3,283,861 - +0.3 3,283,866 + +0.9 3,283,866 + -0.1 3,283,866 + -1.4 3,283,866 + +0.6 3,283,866 + -1.1 3,283,866 - -1.4 3,283,873 - -2.1 3,283,874 - -1.2 3,283,874 - -0.3 3,283,874 - -2.3 3,283,874 - -0.6 3,283,896 + +1.7 3,283,903 + -0.8 3,283,904 - +0.1 3,283,916 + -0.6 3,283,929 + +0.8 3,283,930 - +0.2 3,283,937 - -1.9 3,283,948 - GFF3228 0.10 -0.5 3,283,950 + GFF3228 0.10 +1.1 3,283,950 + GFF3228 0.10 -0.4 3,283,958 + GFF3228 0.11 -0.1 3,283,960 + GFF3228 0.11 -0.1 3,283,961 + GFF3228 0.12 +0.5 3,284,007 - GFF3228 0.17 -2.7 3,284,089 + GFF3228 0.26 -1.1 3,284,120 + GFF3228 0.30 -0.6 3,284,220 + GFF3228 0.41 -0.4 3,284,222 + GFF3228 0.42 +0.6 3,284,232 + GFF3228 0.43 -0.1 3,284,239 + GFF3228 0.44 -0.9 3,284,256 + GFF3228 0.46 -0.1 3,284,261 + GFF3228 0.46 +0.2 3,284,261 + GFF3228 0.46 +0.2 3,284,264 - GFF3228 0.46 +0.7 3,284,264 - GFF3228 0.46 -1.2 3,284,269 - GFF3228 0.47 -0.7 3,284,315 + GFF3228 0.52 -1.0 3,284,323 - GFF3228 0.53 -2.7 3,284,328 - GFF3228 0.54 -1.5 3,284,353 + GFF3228 0.57 -2.0 3,284,370 + GFF3228 0.59 +0.0 3,284,438 - GFF3228 0.67 -1.6 3,284,438 - GFF3228 0.67 +1.1 3,284,443 - GFF3228 0.67 +1.0 3,284,454 + GFF3228 0.68 -1.3 3,284,454 + GFF3228 0.68 +0.7 3,284,463 + GFF3228 0.69 -1.9 3,284,463 - GFF3228 0.69 +1.3 3,284,464 - GFF3228 0.70 +1.2 3,284,475 + GFF3228 0.71 -0.6 3,284,483 + GFF3228 0.72 -1.3 3,284,511 + GFF3228 0.75 +0.0 3,284,521 - GFF3228 0.76 +0.3 3,284,581 + GFF3228 0.83 -0.6 3,284,581 + GFF3228 0.83 +1.1 3,284,594 + GFF3228 0.85 +0.3 3,284,594 + GFF3228 0.85 -0.2 3,284,594 + GFF3228 0.85 +0.6 3,284,594 + GFF3228 0.85 -0.1 3,284,602 - GFF3228 0.85 -0.6 3,284,602 - GFF3228 0.85 -0.2 3,284,602 - GFF3228 0.85 -0.5 3,284,621 - GFF3228 0.88 -0.5 3,284,621 - GFF3228 0.88 -0.7 3,284,621 - GFF3228 0.88 +1.3 3,284,683 - -2.2 3,284,685 + +0.3 3,284,687 + +0.8 3,284,688 - +2.3 3,284,692 + -0.1 3,284,693 - +0.4 3,284,693 - -0.3 3,284,741 - -2.1 3,284,758 + -0.1 3,284,767 - -1.6 3,284,810 - GFF3229 0.20 +0.1 3,284,811 + GFF3229 0.20 +0.5 3,284,825 - GFF3229 0.25 +0.0 3,284,826 + GFF3229 0.25 +1.0 3,284,826 + GFF3229 0.25 +0.0 3,284,826 + GFF3229 0.25 +1.1 3,284,826 + GFF3229 0.25 +1.5 3,284,826 + GFF3229 0.25 -0.5 3,284,834 - GFF3229 0.28 +0.4 3,284,834 - GFF3229 0.28 +0.2 3,284,834 - GFF3229 0.28 +0.0 3,284,834 - GFF3229 0.28 +0.4 3,284,886 + GFF3229 0.46 -0.1 3,284,891 + GFF3229 0.48 +0.8 3,284,891 + GFF3229 0.48 +0.3 3,284,891 + GFF3229 0.48 +0.5 3,284,894 - GFF3229 0.49 +1.2 3,284,901 + GFF3229 0.52 -0.1 3,284,901 + GFF3229 0.52 -0.2 3,284,906 + GFF3229 0.54 -0.1 3,284,909 - GFF3229 0.55 -0.2 3,284,909 - GFF3229 0.55 -1.8 3,284,909 - GFF3229 0.55 -0.5 3,284,909 - GFF3229 0.55 -0.2 3,284,926 + GFF3229 0.61 -0.5 3,284,951 + GFF3229 0.70 +0.2 3,284,964 + GFF3229 0.74 +0.7 3,284,969 + GFF3229 0.76 +0.0 3,284,977 + GFF3229 0.79 -1.3 3,284,985 - GFF3229 0.82 -0.8 3,284,993 + GFF3229 0.84 +0.7 3,285,007 - GFF3229 0.89 -0.5 3,285,007 - GFF3229 0.89 +0.2 3,285,007 - GFF3229 0.89 -1.8 3,285,013 + +0.1 3,285,013 + -0.5 3,285,013 + -1.9 3,285,013 + -0.8 3,285,021 - -0.3 3,285,021 - -0.4 3,285,021 - +1.4 3,285,026 - -0.1 3,285,036 + -2.0 3,285,046 + +0.8 3,285,046 + -0.9 3,285,061 - -1.3 3,285,061 - +0.7 3,285,061 - -1.0 3,285,061 - -0.8 3,285,063 + +0.4 3,285,071 - -0.1 3,285,074 + -2.1 3,285,087 + -0.8 3,285,137 + -0.1 3,285,177 + -0.5 3,285,231 + -1.5 3,285,294 + -0.1 3,285,306 + +1.0 3,285,306 + -0.6 3,285,311 + +0.4 3,285,311 + -0.6 3,285,311 - -1.5 3,285,314 - -0.3 3,285,314 - +1.4 3,285,350 + -0.4 3,285,353 + -0.7 3,285,353 + +0.4 3,285,386 + +0.2 3,285,396 + +0.2 3,285,412 + -0.6 3,285,412 + -0.5 3,285,420 - -0.5 3,285,420 - -0.1 3,285,420 - -2.2 3,285,427 - -0.6 3,285,447 + -0.7 3,285,461 - -0.3 3,285,501 + -0.2 3,285,510 + -1.1 3,285,514 + +1.1 3,285,518 - -0.3 3,285,522 - +1.0 3,285,536 - -1.5 3,285,542 + -0.5 3,285,545 - -0.5 3,285,564 - -1.9 3,285,566 + -0.3 3,285,566 + -0.6 3,285,566 + -0.3 3,285,571 + -1.5 3,285,574 - +1.0 3,285,576 + -1.1 3,285,576 + -0.6 3,285,576 + -0.1 3,285,576 + +0.6 3,285,576 + +1.3 3,285,576 + -0.3 3,285,576 + -1.3 3,285,576 + +0.0 3,285,576 + +0.2 3,285,576 + -0.3 3,285,576 + -1.9 3,285,576 + +0.9 3,285,576 + -0.1 3,285,576 + +0.5 3,285,576 + -1.6 3,285,579 - -0.8 3,285,584 - +1.4 3,285,584 - -1.9 3,285,584 - -1.0 3,285,584 - +0.5 3,285,584 - +0.7 3,285,584 - -0.5 3,285,584 - -1.4 3,285,584 - +0.8 3,285,584 - -1.5 3,285,584 - +0.3 3,285,584 - +0.7 3,285,587 + -0.3 3,285,602 - -1.3 3,285,602 - -0.8 3,285,616 - -1.1 3,285,616 - +0.7 3,285,628 + GFF3230 0.10 -0.6 3,285,630 + GFF3230 0.11 -0.5 3,285,636 - GFF3230 0.14 -2.7 3,285,636 - GFF3230 0.14 +1.0 3,285,636 - GFF3230 0.14 -0.3 3,285,636 - GFF3230 0.14 -1.4 3,285,642 + GFF3230 0.16 -0.7 3,285,653 - GFF3230 0.21 -1.3 3,285,655 + GFF3230 0.22 -2.1 3,285,660 + GFF3230 0.24 +0.2 3,285,668 - GFF3230 0.28 -1.2 3,285,700 + GFF3230 0.42 +0.4 3,285,700 + GFF3230 0.42 -0.5 3,285,714 - GFF3230 0.48 -0.5 3,285,716 + GFF3230 0.49 -1.1 3,285,718 - GFF3230 0.50 -0.8 3,285,732 - GFF3230 0.56 -2.9 3,285,734 + GFF3230 0.57 +0.4 3,285,756 - GFF3230 0.66 -1.4 3,285,763 + GFF3230 0.69 -0.1 3,285,763 + GFF3230 0.69 +0.4 3,285,776 - GFF3230 0.75 +0.0 3,285,779 - GFF3230 0.76 -1.1 3,285,781 - GFF3230 0.77 -1.3 3,285,844 - -1.1 3,285,854 + -0.5 3,285,862 - +0.2 3,285,872 - +0.0 3,285,888 - -0.2 3,285,890 - +0.0 3,285,892 - -1.1 3,285,892 - +0.6 3,285,903 + +0.0 3,285,927 + -0.1 3,285,939 - -0.1 3,285,947 - -0.4 3,285,948 + +0.8 3,285,956 - -0.9 3,285,961 + +1.3 3,285,967 - GFF3231 0.11 -0.7 3,285,973 + GFF3231 0.11 +1.3 3,285,973 + GFF3231 0.11 +0.5 3,285,981 - GFF3231 0.12 -1.2 3,285,981 - GFF3231 0.12 -1.3 3,285,981 - GFF3231 0.12 +0.5 3,285,981 - GFF3231 0.12 +0.0 3,285,988 + GFF3231 0.13 +0.3 3,285,996 - GFF3231 0.14 +0.4 3,286,008 + GFF3231 0.16 -0.4 3,286,008 + GFF3231 0.16 +0.4 3,286,008 + GFF3231 0.16 -2.2 3,286,008 + GFF3231 0.16 +0.5 3,286,008 + GFF3231 0.16 -0.2 3,286,008 + GFF3231 0.16 +0.3 3,286,008 + GFF3231 0.16 -0.5 3,286,008 + GFF3231 0.16 +0.0 3,286,008 + GFF3231 0.16 -0.6 3,286,008 + GFF3231 0.16 +1.2 3,286,008 + GFF3231 0.16 -2.9 3,286,008 + GFF3231 0.16 +0.0 3,286,008 + GFF3231 0.16 -0.7 3,286,009 + GFF3231 0.16 -0.8 3,286,009 + GFF3231 0.16 +1.3 3,286,009 + GFF3231 0.16 +0.9 3,286,009 + GFF3231 0.16 -0.1 3,286,009 + GFF3231 0.16 +0.4 3,286,016 - GFF3231 0.16 -0.1 3,286,016 - GFF3231 0.16 -0.6 3,286,016 - GFF3231 0.16 -1.4 3,286,016 - GFF3231 0.16 -0.7 3,286,016 - GFF3231 0.16 -0.1 3,286,017 - GFF3231 0.17 +1.3 3,286,017 - GFF3231 0.17 +0.9 3,286,017 - GFF3231 0.17 +0.2 3,286,017 - GFF3231 0.17 -1.8 3,286,027 - GFF3231 0.18 +0.3 3,286,035 - GFF3231 0.19 -1.3
Or see this region's nucleotide sequence