Strain Fitness in Sphingobium sp. HT1-2 around GFF226

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF223 and GFF224 are separated by 154 nucleotidesGFF224 and GFF225 are separated by 75 nucleotidesGFF225 and GFF226 overlap by 39 nucleotidesGFF226 and GFF227 are separated by 44 nucleotidesGFF227 and GFF228 are separated by 18 nucleotides GFF223 - LSU ribosomal protein L28p @ LSU ribosomal protein L28p, zinc-independent, at 228,952 to 229,245 GFF223 GFF224 - hypothetical protein, at 229,400 to 229,621 GFF224 GFF225 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.33), at 229,697 to 230,155 GFF225 GFF226 - hypothetical protein, at 230,117 to 232,030 GFF226 GFF227 - hypothetical protein, at 232,075 to 232,491 GFF227 GFF228 - Transcriptional regulator, AraC family, at 232,510 to 233,406 GFF228 Position (kb) 230 231 232 233Strain fitness (log2 ratio) -2 -1 0 1 2at 229.311 kb on + strandat 229.329 kb on + strandat 229.370 kb on + strandat 229.378 kb on + strandat 229.378 kb on - strandat 229.380 kb on + strandat 229.383 kb on + strandat 229.385 kb on + strandat 229.388 kb on + strandat 229.391 kb on - strandat 229.398 kb on - strandat 229.477 kb on + strand, within GFF224at 229.540 kb on - strand, within GFF224at 229.679 kb on + strandat 229.681 kb on + strandat 230.158 kb on + strandat 230.163 kb on + strandat 230.163 kb on + strandat 230.163 kb on + strandat 230.163 kb on + strandat 230.163 kb on + strandat 230.171 kb on - strandat 230.175 kb on + strandat 230.175 kb on + strandat 230.175 kb on + strandat 230.183 kb on - strandat 230.248 kb on - strandat 230.355 kb on - strand, within GFF226at 230.658 kb on - strand, within GFF226at 230.661 kb on - strand, within GFF226at 230.729 kb on - strand, within GFF226at 230.748 kb on - strand, within GFF226at 230.867 kb on - strand, within GFF226at 230.906 kb on - strand, within GFF226at 230.906 kb on - strand, within GFF226at 230.949 kb on + strand, within GFF226at 230.963 kb on - strand, within GFF226at 231.064 kb on - strand, within GFF226at 231.125 kb on - strand, within GFF226at 231.168 kb on + strand, within GFF226at 231.233 kb on + strand, within GFF226at 231.241 kb on + strand, within GFF226at 231.241 kb on - strand, within GFF226at 231.249 kb on - strand, within GFF226at 231.515 kb on + strand, within GFF226at 231.515 kb on + strand, within GFF226at 231.557 kb on + strand, within GFF226at 231.559 kb on + strand, within GFF226at 231.657 kb on + strand, within GFF226at 231.662 kb on + strand, within GFF226at 231.670 kb on - strand, within GFF226at 231.670 kb on - strand, within GFF226at 231.737 kb on - strand, within GFF226at 231.793 kb on + strand, within GFF226at 231.793 kb on + strand, within GFF226at 231.798 kb on + strand, within GFF226at 231.801 kb on - strand, within GFF226at 231.801 kb on - strand, within GFF226at 231.845 kb on + strandat 231.873 kb on - strandat 231.943 kb on - strandat 232.014 kb on - strandat 232.038 kb on + strandat 232.048 kb on + strandat 232.056 kb on - strandat 232.068 kb on - strandat 232.095 kb on + strandat 232.095 kb on + strandat 232.113 kb on - strandat 232.124 kb on - strand, within GFF227at 232.500 kb on - strandat 232.500 kb on - strandat 232.500 kb on - strandat 232.536 kb on - strandat 232.561 kb on - strandat 232.566 kb on - strandat 232.600 kb on + strand, within GFF228at 232.610 kb on + strand, within GFF228at 232.615 kb on + strand, within GFF228at 232.623 kb on - strand, within GFF228at 232.667 kb on - strand, within GFF228at 232.688 kb on + strand, within GFF228at 232.720 kb on + strand, within GFF228at 232.723 kb on - strand, within GFF228at 232.793 kb on - strand, within GFF228at 232.793 kb on - strand, within GFF228at 232.801 kb on - strand, within GFF228at 232.803 kb on + strand, within GFF228at 232.811 kb on - strand, within GFF228at 232.811 kb on - strand, within GFF228at 232.817 kb on + strand, within GFF228at 232.817 kb on + strand, within GFF228at 232.853 kb on + strand, within GFF228at 232.861 kb on - strand, within GFF228at 232.902 kb on - strand, within GFF228at 232.950 kb on + strand, within GFF228at 232.950 kb on + strand, within GFF228at 232.955 kb on + strand, within GFF228at 232.958 kb on + strand, within GFF228at 232.958 kb on + strand, within GFF228at 232.960 kb on + strand, within GFF228at 232.963 kb on - strand, within GFF228at 232.966 kb on - strand, within GFF228at 232.969 kb on + strand, within GFF228at 232.969 kb on + strand, within GFF228at 232.977 kb on + strand, within GFF228at 232.984 kb on - strand, within GFF228at 232.999 kb on + strand, within GFF228at 233.007 kb on + strand, within GFF228at 233.007 kb on - strand, within GFF228at 233.007 kb on - strand, within GFF228at 233.015 kb on - strand, within GFF228at 233.018 kb on + strand, within GFF228at 233.022 kb on + strand, within GFF228at 233.026 kb on + strand, within GFF228at 233.026 kb on + strand, within GFF228at 233.030 kb on - strand, within GFF228

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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229,311 + -0.8
229,329 + -0.8
229,370 + +0.9
229,378 + +0.5
229,378 - -1.4
229,380 + -2.0
229,383 + -0.0
229,385 + +0.6
229,388 + -1.0
229,391 - +1.1
229,398 - -0.7
229,477 + GFF224 0.35 -0.9
229,540 - GFF224 0.63 +2.1
229,679 + -0.2
229,681 + -0.2
230,158 + +0.6
230,163 + +0.7
230,163 + -0.6
230,163 + -0.1
230,163 + +0.4
230,163 + -0.0
230,171 - -0.3
230,175 + -0.3
230,175 + -0.0
230,175 + -0.6
230,183 - +0.5
230,248 - -1.7
230,355 - GFF226 0.12 +0.5
230,658 - GFF226 0.28 +0.5
230,661 - GFF226 0.28 +1.0
230,729 - GFF226 0.32 +1.8
230,748 - GFF226 0.33 +0.5
230,867 - GFF226 0.39 -0.5
230,906 - GFF226 0.41 -0.5
230,906 - GFF226 0.41 -0.0
230,949 + GFF226 0.43 -0.3
230,963 - GFF226 0.44 +2.2
231,064 - GFF226 0.49 +0.2
231,125 - GFF226 0.53 -0.0
231,168 + GFF226 0.55 +0.2
231,233 + GFF226 0.58 +0.5
231,241 + GFF226 0.59 -0.6
231,241 - GFF226 0.59 +0.5
231,249 - GFF226 0.59 -0.0
231,515 + GFF226 0.73 +0.2
231,515 + GFF226 0.73 +0.5
231,557 + GFF226 0.75 +0.5
231,559 + GFF226 0.75 -0.0
231,657 + GFF226 0.80 -0.5
231,662 + GFF226 0.81 +0.5
231,670 - GFF226 0.81 -0.0
231,670 - GFF226 0.81 +0.5
231,737 - GFF226 0.85 +0.5
231,793 + GFF226 0.88 +0.5
231,793 + GFF226 0.88 +0.5
231,798 + GFF226 0.88 -0.8
231,801 - GFF226 0.88 -0.5
231,801 - GFF226 0.88 -0.3
231,845 + -0.0
231,873 - +1.2
231,943 - -0.3
232,014 - +0.2
232,038 + -0.5
232,048 + +0.3
232,056 - +0.2
232,068 - +1.2
232,095 + -1.2
232,095 + +2.3
232,113 - +0.7
232,124 - GFF227 0.12 +2.0
232,500 - +0.6
232,500 - +0.1
232,500 - -0.4
232,536 - +0.1
232,561 - -0.3
232,566 - +0.4
232,600 + GFF228 0.10 +0.2
232,610 + GFF228 0.11 -1.0
232,615 + GFF228 0.12 +1.5
232,623 - GFF228 0.13 -1.3
232,667 - GFF228 0.18 +0.2
232,688 + GFF228 0.20 +0.2
232,720 + GFF228 0.23 -0.2
232,723 - GFF228 0.24 -0.5
232,793 - GFF228 0.32 -0.4
232,793 - GFF228 0.32 +1.1
232,801 - GFF228 0.32 +0.2
232,803 + GFF228 0.33 -0.3
232,811 - GFF228 0.34 -0.8
232,811 - GFF228 0.34 -0.8
232,817 + GFF228 0.34 +0.7
232,817 + GFF228 0.34 +0.9
232,853 + GFF228 0.38 +0.8
232,861 - GFF228 0.39 -0.5
232,902 - GFF228 0.44 +0.4
232,950 + GFF228 0.49 -0.3
232,950 + GFF228 0.49 +1.2
232,955 + GFF228 0.50 -0.6
232,958 + GFF228 0.50 -1.1
232,958 + GFF228 0.50 +1.0
232,960 + GFF228 0.50 +1.1
232,963 - GFF228 0.51 -0.5
232,966 - GFF228 0.51 +1.0
232,969 + GFF228 0.51 -0.5
232,969 + GFF228 0.51 -1.3
232,977 + GFF228 0.52 +0.7
232,984 - GFF228 0.53 +0.9
232,999 + GFF228 0.55 -1.8
233,007 + GFF228 0.55 -0.3
233,007 - GFF228 0.55 -1.5
233,007 - GFF228 0.55 -0.6
233,015 - GFF228 0.56 +1.1
233,018 + GFF228 0.57 -1.0
233,022 + GFF228 0.57 +0.4
233,026 + GFF228 0.58 -0.4
233,026 + GFF228 0.58 +1.5
233,030 - GFF228 0.58 -0.2

Or see this region's nucleotide sequence