Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF218 and GFF219 are separated by 213 nucleotides GFF219 and GFF220 are separated by 83 nucleotides GFF220 and GFF221 are separated by 56 nucleotides GFF221 and GFF222 are separated by 183 nucleotides
GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373
GFF218
GFF219 - hypothetical protein, at 226,587 to 226,805
GFF219
GFF220 - hypothetical protein, at 226,889 to 227,122
GFF220
GFF221 - hypothetical protein, at 227,179 to 227,604
GFF221
GFF222 - hypothetical protein, at 227,788 to 228,771
GFF222
Position (kb) 226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 225.689 kb on - strand, within GFF218 at 225.700 kb on + strand, within GFF218 at 225.708 kb on + strand, within GFF218 at 225.716 kb on - strand, within GFF218 at 225.778 kb on - strand, within GFF218 at 225.790 kb on + strand, within GFF218 at 225.852 kb on - strand, within GFF218 at 225.933 kb on + strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.973 kb on + strand, within GFF218 at 226.034 kb on - strand, within GFF218 at 226.075 kb on - strand, within GFF218 at 226.079 kb on - strand, within GFF218 at 226.094 kb on - strand, within GFF218 at 226.140 kb on + strand, within GFF218 at 226.141 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.249 kb on + strand, within GFF218 at 226.269 kb on - strand, within GFF218 at 226.300 kb on + strand at 226.308 kb on - strand at 226.353 kb on - strand at 226.450 kb on + strand at 226.458 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.490 kb on + strand at 226.490 kb on + strand at 226.498 kb on - strand at 226.639 kb on - strand, within GFF219 at 226.700 kb on - strand, within GFF219 at 226.748 kb on + strand, within GFF219 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 227.060 kb on - strand, within GFF220 at 227.061 kb on - strand, within GFF220 at 227.170 kb on + strand at 227.270 kb on + strand, within GFF221 at 227.275 kb on + strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.301 kb on + strand, within GFF221 at 227.334 kb on - strand, within GFF221 at 227.343 kb on + strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.437 kb on - strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.511 kb on - strand, within GFF221 at 227.549 kb on - strand, within GFF221 at 227.606 kb on + strand at 227.669 kb on + strand at 227.669 kb on + strand at 227.718 kb on + strand at 227.726 kb on - strand at 227.726 kb on - strand at 227.726 kb on - strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.750 kb on + strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.792 kb on + strand at 227.800 kb on - strand at 227.800 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 225,689 - GFF218 0.30 +0.7 225,700 + GFF218 0.31 +0.4 225,708 + GFF218 0.31 +1.8 225,716 - GFF218 0.32 +0.7 225,778 - GFF218 0.39 +0.0 225,790 + GFF218 0.40 -0.8 225,852 - GFF218 0.46 -0.8 225,933 + GFF218 0.55 -1.6 225,941 - GFF218 0.55 -0.4 225,941 - GFF218 0.55 +0.8 225,973 + GFF218 0.59 -1.7 226,034 - GFF218 0.65 +0.3 226,075 - GFF218 0.69 -1.7 226,079 - GFF218 0.70 -0.6 226,094 - GFF218 0.71 +1.2 226,140 + GFF218 0.76 +0.5 226,141 + GFF218 0.76 -0.3 226,154 + GFF218 0.77 -2.3 226,154 + GFF218 0.77 -0.2 226,154 + GFF218 0.77 -0.9 226,249 + GFF218 0.87 -1.5 226,269 - GFF218 0.89 -0.0 226,300 + -0.2 226,308 - +1.2 226,353 - -0.0 226,450 + +1.2 226,458 - -0.3 226,488 - +1.2 226,488 - -0.5 226,488 - +1.2 226,488 - -0.7 226,488 - +2.7 226,488 - -1.3 226,488 - +1.2 226,488 - +0.4 226,490 + +1.5 226,490 + -0.1 226,498 - -0.2 226,639 - GFF219 0.24 -0.9 226,700 - GFF219 0.52 +0.0 226,748 + GFF219 0.74 -0.2 226,976 + GFF220 0.37 +0.6 226,976 + GFF220 0.37 -0.1 226,976 + GFF220 0.37 +0.1 226,976 + GFF220 0.37 -2.4 226,976 + GFF220 0.37 +0.9 227,060 - GFF220 0.73 +0.3 227,061 - GFF220 0.74 -0.9 227,170 + +1.1 227,270 + GFF221 0.21 +0.1 227,275 + GFF221 0.23 +2.7 227,283 - GFF221 0.24 -1.7 227,283 - GFF221 0.24 -0.7 227,283 - GFF221 0.24 +2.3 227,301 + GFF221 0.29 +1.9 227,334 - GFF221 0.36 +0.0 227,343 + GFF221 0.38 +0.1 227,351 - GFF221 0.40 -0.3 227,437 - GFF221 0.61 -2.2 227,503 + GFF221 0.76 -0.0 227,503 + GFF221 0.76 +1.3 227,503 + GFF221 0.76 -0.3 227,511 - GFF221 0.78 +0.2 227,549 - GFF221 0.87 -1.8 227,606 + +0.5 227,669 + -0.6 227,669 + +0.3 227,718 + -0.5 227,726 - -0.3 227,726 - -1.7 227,726 - +0.4 227,745 + +0.8 227,745 + +0.4 227,745 + +1.5 227,750 + +0.6 227,753 - -1.4 227,753 - -0.4 227,753 - +0.2 227,753 - -0.3 227,792 + -0.3 227,800 - +0.6 227,800 - -0.4
Or see this region's nucleotide sequence