Strain Fitness in Sphingobium sp. HT1-2 around GFF217

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF216 and GFF217 overlap by 4 nucleotidesGFF217 and GFF218 overlap by 4 nucleotides GFF216 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), N-terminal domain, at 223,430 to 224,476 GFF216 GFF217 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 224,473 to 225,405 GFF217 GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373 GFF218 Position (kb) 224 225 226Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 223.506 kb on + strandat 223.509 kb on - strandat 223.509 kb on - strandat 223.509 kb on - strandat 223.509 kb on - strandat 223.509 kb on - strandat 223.509 kb on - strandat 223.563 kb on + strand, within GFF216at 223.620 kb on - strand, within GFF216at 223.642 kb on - strand, within GFF216at 223.671 kb on + strand, within GFF216at 223.677 kb on + strand, within GFF216at 223.677 kb on + strand, within GFF216at 223.684 kb on - strand, within GFF216at 223.689 kb on - strand, within GFF216at 223.707 kb on + strand, within GFF216at 223.727 kb on + strand, within GFF216at 223.727 kb on + strand, within GFF216at 223.730 kb on - strand, within GFF216at 223.732 kb on + strand, within GFF216at 223.732 kb on + strand, within GFF216at 223.732 kb on + strand, within GFF216at 223.732 kb on + strand, within GFF216at 223.740 kb on - strand, within GFF216at 223.740 kb on - strand, within GFF216at 223.740 kb on - strand, within GFF216at 223.740 kb on - strand, within GFF216at 223.740 kb on - strand, within GFF216at 223.751 kb on + strand, within GFF216at 223.759 kb on - strand, within GFF216at 223.764 kb on - strand, within GFF216at 223.764 kb on - strand, within GFF216at 223.771 kb on + strand, within GFF216at 223.771 kb on + strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.779 kb on - strand, within GFF216at 223.831 kb on - strand, within GFF216at 223.854 kb on - strand, within GFF216at 223.874 kb on - strand, within GFF216at 223.926 kb on - strand, within GFF216at 224.055 kb on - strand, within GFF216at 224.061 kb on - strand, within GFF216at 224.186 kb on + strand, within GFF216at 224.194 kb on - strand, within GFF216at 224.194 kb on - strand, within GFF216at 224.244 kb on + strand, within GFF216at 224.246 kb on - strand, within GFF216at 224.246 kb on - strand, within GFF216at 224.274 kb on - strand, within GFF216at 224.288 kb on - strand, within GFF216at 224.298 kb on + strand, within GFF216at 224.304 kb on + strand, within GFF216at 224.312 kb on - strand, within GFF216at 224.331 kb on - strand, within GFF216at 224.353 kb on + strand, within GFF216at 224.353 kb on + strand, within GFF216at 224.388 kb on - strandat 224.406 kb on + strandat 224.408 kb on + strandat 224.416 kb on - strandat 224.434 kb on - strandat 224.514 kb on + strandat 224.539 kb on + strandat 224.539 kb on + strandat 224.547 kb on - strandat 224.547 kb on - strandat 224.608 kb on - strand, within GFF217at 224.666 kb on - strand, within GFF217at 224.697 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.702 kb on + strand, within GFF217at 224.710 kb on - strand, within GFF217at 224.710 kb on - strand, within GFF217at 224.710 kb on - strand, within GFF217at 224.730 kb on - strand, within GFF217at 224.797 kb on - strand, within GFF217at 224.844 kb on - strand, within GFF217at 224.863 kb on + strand, within GFF217at 224.975 kb on + strand, within GFF217at 224.975 kb on + strand, within GFF217at 225.065 kb on - strand, within GFF217at 225.078 kb on - strand, within GFF217at 225.100 kb on - strand, within GFF217at 225.110 kb on + strand, within GFF217at 225.136 kb on + strand, within GFF217at 225.144 kb on - strand, within GFF217at 225.188 kb on + strand, within GFF217at 225.189 kb on + strand, within GFF217at 225.197 kb on - strand, within GFF217at 225.222 kb on + strand, within GFF217at 225.278 kb on + strand, within GFF217at 225.351 kb on + strandat 225.361 kb on + strandat 225.374 kb on + strandat 225.395 kb on + strandat 225.403 kb on - strandat 225.491 kb on + strandat 225.509 kb on + strand, within GFF218at 225.566 kb on - strand, within GFF218at 225.583 kb on - strand, within GFF218at 225.689 kb on - strand, within GFF218at 225.700 kb on + strand, within GFF218at 225.708 kb on + strand, within GFF218at 225.716 kb on - strand, within GFF218at 225.778 kb on - strand, within GFF218at 225.790 kb on + strand, within GFF218at 225.852 kb on - strand, within GFF218at 225.933 kb on + strand, within GFF218at 225.941 kb on - strand, within GFF218at 225.941 kb on - strand, within GFF218at 225.973 kb on + strand, within GFF218at 226.034 kb on - strand, within GFF218at 226.075 kb on - strand, within GFF218at 226.079 kb on - strand, within GFF218at 226.094 kb on - strand, within GFF218at 226.140 kb on + strand, within GFF218at 226.141 kb on + strand, within GFF218at 226.154 kb on + strand, within GFF218at 226.154 kb on + strand, within GFF218at 226.154 kb on + strand, within GFF218at 226.249 kb on + strand, within GFF218at 226.269 kb on - strand, within GFF218at 226.300 kb on + strandat 226.308 kb on - strandat 226.353 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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223,506 + -0.9
223,509 - -1.5
223,509 - -1.4
223,509 - -2.5
223,509 - -1.9
223,509 - -2.0
223,509 - -0.2
223,563 + GFF216 0.13 -2.5
223,620 - GFF216 0.18 -1.5
223,642 - GFF216 0.20 -1.5
223,671 + GFF216 0.23 -1.8
223,677 + GFF216 0.24 -0.9
223,677 + GFF216 0.24 -3.0
223,684 - GFF216 0.24 -1.0
223,689 - GFF216 0.25 -0.5
223,707 + GFF216 0.26 -2.2
223,727 + GFF216 0.28 +0.1
223,727 + GFF216 0.28 -2.4
223,730 - GFF216 0.29 -0.5
223,732 + GFF216 0.29 -2.0
223,732 + GFF216 0.29 -1.6
223,732 + GFF216 0.29 -1.3
223,732 + GFF216 0.29 -1.4
223,740 - GFF216 0.30 -0.9
223,740 - GFF216 0.30 -1.7
223,740 - GFF216 0.30 -1.0
223,740 - GFF216 0.30 -1.0
223,740 - GFF216 0.30 -1.6
223,751 + GFF216 0.31 -2.0
223,759 - GFF216 0.31 -2.3
223,764 - GFF216 0.32 -0.8
223,764 - GFF216 0.32 -1.4
223,771 + GFF216 0.33 -2.5
223,771 + GFF216 0.33 -2.7
223,779 - GFF216 0.33 +0.2
223,779 - GFF216 0.33 -2.2
223,779 - GFF216 0.33 -2.6
223,779 - GFF216 0.33 -2.5
223,779 - GFF216 0.33 -1.3
223,779 - GFF216 0.33 -1.9
223,831 - GFF216 0.38 -2.5
223,854 - GFF216 0.40 -1.9
223,874 - GFF216 0.42 -1.5
223,926 - GFF216 0.47 -1.8
224,055 - GFF216 0.60 -0.9
224,061 - GFF216 0.60 -2.2
224,186 + GFF216 0.72 -1.2
224,194 - GFF216 0.73 -1.3
224,194 - GFF216 0.73 +0.5
224,244 + GFF216 0.78 -1.5
224,246 - GFF216 0.78 -0.9
224,246 - GFF216 0.78 -1.4
224,274 - GFF216 0.81 -1.0
224,288 - GFF216 0.82 -2.7
224,298 + GFF216 0.83 -2.0
224,304 + GFF216 0.83 -2.3
224,312 - GFF216 0.84 -0.6
224,331 - GFF216 0.86 -2.8
224,353 + GFF216 0.88 -1.4
224,353 + GFF216 0.88 -1.9
224,388 - -0.0
224,406 + -2.9
224,408 + -1.0
224,416 - -2.6
224,434 - -2.1
224,514 + -1.9
224,539 + -1.9
224,539 + -2.0
224,547 - -1.2
224,547 - -1.6
224,608 - GFF217 0.14 -2.5
224,666 - GFF217 0.21 -3.6
224,697 + GFF217 0.24 -2.4
224,702 + GFF217 0.25 -2.7
224,702 + GFF217 0.25 -2.3
224,702 + GFF217 0.25 -2.5
224,702 + GFF217 0.25 -1.6
224,702 + GFF217 0.25 -3.2
224,702 + GFF217 0.25 -2.7
224,710 - GFF217 0.25 -0.6
224,710 - GFF217 0.25 -2.3
224,710 - GFF217 0.25 -2.4
224,730 - GFF217 0.28 -0.8
224,797 - GFF217 0.35 -2.4
224,844 - GFF217 0.40 -1.5
224,863 + GFF217 0.42 -2.9
224,975 + GFF217 0.54 -3.6
224,975 + GFF217 0.54 +0.5
225,065 - GFF217 0.63 -0.8
225,078 - GFF217 0.65 -1.9
225,100 - GFF217 0.67 -2.2
225,110 + GFF217 0.68 -1.2
225,136 + GFF217 0.71 -1.3
225,144 - GFF217 0.72 -1.1
225,188 + GFF217 0.77 -2.3
225,189 + GFF217 0.77 -0.6
225,197 - GFF217 0.78 -2.1
225,222 + GFF217 0.80 -1.5
225,278 + GFF217 0.86 -2.5
225,351 + -1.2
225,361 + -1.3
225,374 + -1.4
225,395 + -2.8
225,403 - -0.9
225,491 + -0.0
225,509 + GFF218 0.11 -0.2
225,566 - GFF218 0.17 -1.5
225,583 - GFF218 0.19 +0.0
225,689 - GFF218 0.30 +0.7
225,700 + GFF218 0.31 +0.4
225,708 + GFF218 0.31 +1.8
225,716 - GFF218 0.32 +0.7
225,778 - GFF218 0.39 +0.0
225,790 + GFF218 0.40 -0.8
225,852 - GFF218 0.46 -0.8
225,933 + GFF218 0.55 -1.6
225,941 - GFF218 0.55 -0.4
225,941 - GFF218 0.55 +0.8
225,973 + GFF218 0.59 -1.7
226,034 - GFF218 0.65 +0.3
226,075 - GFF218 0.69 -1.7
226,079 - GFF218 0.70 -0.6
226,094 - GFF218 0.71 +1.2
226,140 + GFF218 0.76 +0.5
226,141 + GFF218 0.76 -0.3
226,154 + GFF218 0.77 -2.3
226,154 + GFF218 0.77 -0.2
226,154 + GFF218 0.77 -0.9
226,249 + GFF218 0.87 -1.5
226,269 - GFF218 0.89 -0.0
226,300 + -0.2
226,308 - +1.2
226,353 - -0.0

Or see this region's nucleotide sequence