Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1971 and GFF1972 are separated by 5 nucleotides GFF1972 and GFF1973 overlap by 4 nucleotides GFF1973 and GFF1974 are separated by 38 nucleotides GFF1974 and GFF1975 are separated by 21 nucleotides
GFF1971 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4), at 1,995,404 to 1,996,363
GFF1971
GFF1972 - Homoserine kinase (EC 2.7.1.39), at 1,996,369 to 1,997,346
GFF1972
GFF1973 - Ribonuclease HI (EC 3.1.26.4), at 1,997,343 to 1,997,789
GFF1973
GFF1974 - UPF0339 protein YegP.N, at 1,997,828 to 1,998,004
GFF1974
GFF1975 - Glycine/D-amino acid oxidases (deaminating), at 1,998,026 to 1,999,129
GFF1975
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1995.388 kb on + strand at 1996.337 kb on - strand at 1996.347 kb on + strand at 1996.355 kb on - strand at 1996.366 kb on + strand at 1996.401 kb on + strand at 1996.401 kb on + strand at 1996.429 kb on - strand at 1996.430 kb on - strand at 1996.514 kb on - strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.523 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.528 kb on + strand, within GFF1972 at 1996.531 kb on - strand, within GFF1972 at 1996.531 kb on - strand, within GFF1972 at 1996.564 kb on + strand, within GFF1972 at 1996.594 kb on - strand, within GFF1972 at 1996.610 kb on + strand, within GFF1972 at 1996.613 kb on + strand, within GFF1972 at 1996.616 kb on - strand, within GFF1972 at 1996.767 kb on + strand, within GFF1972 at 1996.767 kb on + strand, within GFF1972 at 1996.772 kb on + strand, within GFF1972 at 1996.772 kb on + strand, within GFF1972 at 1996.775 kb on - strand, within GFF1972 at 1996.777 kb on + strand, within GFF1972 at 1996.785 kb on - strand, within GFF1972 at 1996.818 kb on + strand, within GFF1972 at 1996.955 kb on + strand, within GFF1972 at 1996.955 kb on + strand, within GFF1972 at 1996.963 kb on - strand, within GFF1972 at 1996.974 kb on - strand, within GFF1972 at 1996.978 kb on + strand, within GFF1972 at 1996.986 kb on - strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.045 kb on + strand, within GFF1972 at 1997.147 kb on + strand, within GFF1972 at 1997.180 kb on + strand, within GFF1972 at 1997.190 kb on + strand, within GFF1972 at 1997.198 kb on + strand, within GFF1972 at 1997.198 kb on - strand, within GFF1972 at 1997.203 kb on + strand, within GFF1972 at 1997.203 kb on + strand, within GFF1972 at 1997.211 kb on - strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.215 kb on + strand, within GFF1972 at 1997.223 kb on - strand, within GFF1972 at 1997.235 kb on - strand, within GFF1972 at 1997.287 kb on + strand at 1997.324 kb on + strand at 1997.339 kb on + strand at 1997.342 kb on - strand at 1997.347 kb on - strand at 1997.347 kb on - strand at 1997.361 kb on + strand at 1997.369 kb on - strand at 1997.369 kb on - strand at 1997.382 kb on - strand at 1997.390 kb on + strand, within GFF1973 at 1997.390 kb on + strand, within GFF1973 at 1997.421 kb on + strand, within GFF1973 at 1997.429 kb on - strand, within GFF1973 at 1997.449 kb on - strand, within GFF1973 at 1997.546 kb on + strand, within GFF1973 at 1997.682 kb on + strand, within GFF1973 at 1997.758 kb on + strand at 1997.764 kb on - strand at 1997.769 kb on + strand at 1997.803 kb on - strand at 1997.899 kb on + strand, within GFF1974 at 1997.900 kb on - strand, within GFF1974 at 1997.905 kb on - strand, within GFF1974 at 1997.906 kb on + strand, within GFF1974 at 1997.907 kb on - strand, within GFF1974 at 1997.970 kb on + strand, within GFF1974 at 1998.011 kb on + strand at 1998.011 kb on - strand at 1998.045 kb on + strand at 1998.088 kb on - strand at 1998.088 kb on - strand at 1998.093 kb on - strand at 1998.093 kb on - strand at 1998.103 kb on - strand at 1998.103 kb on - strand at 1998.111 kb on - strand at 1998.116 kb on - strand at 1998.116 kb on - strand at 1998.135 kb on + strand at 1998.176 kb on + strand, within GFF1975 at 1998.197 kb on + strand, within GFF1975 at 1998.220 kb on + strand, within GFF1975 at 1998.230 kb on + strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.233 kb on - strand, within GFF1975 at 1998.235 kb on + strand, within GFF1975 at 1998.290 kb on - strand, within GFF1975 at 1998.292 kb on + strand, within GFF1975 at 1998.319 kb on - strand, within GFF1975 at 1998.327 kb on - strand, within GFF1975
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,995,388 + +1.5 1,995,388 + -0.5 1,995,388 + -0.1 1,995,388 + +0.8 1,995,388 + -0.5 1,996,337 - -2.7 1,996,347 + -0.8 1,996,355 - -0.6 1,996,366 + -1.4 1,996,401 + -1.7 1,996,401 + -1.8 1,996,429 - -1.1 1,996,430 - -0.6 1,996,514 - GFF1972 0.15 -1.5 1,996,523 + GFF1972 0.16 -0.7 1,996,523 + GFF1972 0.16 -2.0 1,996,528 + GFF1972 0.16 -0.9 1,996,528 + GFF1972 0.16 -1.9 1,996,528 + GFF1972 0.16 -2.2 1,996,528 + GFF1972 0.16 -2.5 1,996,528 + GFF1972 0.16 -0.9 1,996,531 - GFF1972 0.17 -2.5 1,996,531 - GFF1972 0.17 -1.6 1,996,564 + GFF1972 0.20 -2.1 1,996,594 - GFF1972 0.23 -2.5 1,996,610 + GFF1972 0.25 -1.4 1,996,613 + GFF1972 0.25 -2.2 1,996,616 - GFF1972 0.25 -1.4 1,996,767 + GFF1972 0.41 -0.7 1,996,767 + GFF1972 0.41 -1.7 1,996,772 + GFF1972 0.41 -1.6 1,996,772 + GFF1972 0.41 -2.7 1,996,775 - GFF1972 0.42 -1.1 1,996,777 + GFF1972 0.42 -2.6 1,996,785 - GFF1972 0.43 -1.3 1,996,818 + GFF1972 0.46 -1.5 1,996,955 + GFF1972 0.60 -2.0 1,996,955 + GFF1972 0.60 -2.9 1,996,963 - GFF1972 0.61 -1.0 1,996,974 - GFF1972 0.62 -2.8 1,996,978 + GFF1972 0.62 -0.6 1,996,986 - GFF1972 0.63 -1.3 1,997,045 + GFF1972 0.69 -1.3 1,997,045 + GFF1972 0.69 -1.7 1,997,045 + GFF1972 0.69 -1.0 1,997,045 + GFF1972 0.69 -2.0 1,997,045 + GFF1972 0.69 -0.7 1,997,147 + GFF1972 0.80 -1.7 1,997,180 + GFF1972 0.83 -1.2 1,997,190 + GFF1972 0.84 -1.6 1,997,198 + GFF1972 0.85 -1.0 1,997,198 - GFF1972 0.85 -1.2 1,997,203 + GFF1972 0.85 -2.1 1,997,203 + GFF1972 0.85 -2.6 1,997,211 - GFF1972 0.86 -0.6 1,997,215 + GFF1972 0.87 -3.2 1,997,215 + GFF1972 0.87 -1.7 1,997,223 - GFF1972 0.87 +0.0 1,997,235 - GFF1972 0.89 -1.0 1,997,287 + -2.2 1,997,324 + -3.6 1,997,339 + -0.5 1,997,342 - -1.8 1,997,347 - -1.9 1,997,347 - -1.1 1,997,361 + +0.7 1,997,369 - +0.0 1,997,369 - +0.6 1,997,382 - -1.1 1,997,390 + GFF1973 0.11 +0.4 1,997,390 + GFF1973 0.11 -0.2 1,997,421 + GFF1973 0.17 +1.9 1,997,429 - GFF1973 0.19 +2.6 1,997,449 - GFF1973 0.24 +0.3 1,997,546 + GFF1973 0.45 +2.0 1,997,682 + GFF1973 0.76 -0.3 1,997,758 + -1.1 1,997,764 - +0.2 1,997,769 + -1.0 1,997,803 - -1.5 1,997,899 + GFF1974 0.40 +0.1 1,997,900 - GFF1974 0.41 -0.3 1,997,905 - GFF1974 0.44 +0.2 1,997,906 + GFF1974 0.44 +0.2 1,997,907 - GFF1974 0.45 -0.2 1,997,970 + GFF1974 0.80 -0.8 1,998,011 + +0.8 1,998,011 - +0.4 1,998,045 + +0.6 1,998,088 - -1.9 1,998,088 - -1.7 1,998,093 - -0.5 1,998,093 - +0.6 1,998,103 - -2.3 1,998,103 - -3.1 1,998,111 - +0.4 1,998,116 - +0.7 1,998,116 - +0.1 1,998,135 + -0.4 1,998,176 + GFF1975 0.14 +1.4 1,998,197 + GFF1975 0.15 +2.1 1,998,220 + GFF1975 0.18 -1.4 1,998,230 + GFF1975 0.18 -1.4 1,998,233 - GFF1975 0.19 +0.5 1,998,233 - GFF1975 0.19 -0.1 1,998,233 - GFF1975 0.19 +0.0 1,998,235 + GFF1975 0.19 +0.1 1,998,290 - GFF1975 0.24 +0.8 1,998,292 + GFF1975 0.24 -0.1 1,998,319 - GFF1975 0.27 -0.7 1,998,327 - GFF1975 0.27 -0.2
Or see this region's nucleotide sequence