Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1857 and GFF1858 overlap by 4 nucleotides GFF1858 and GFF1859 overlap by 4 nucleotides GFF1859 and GFF1860 overlap by 1 nucleotides
GFF1857 - Precorrin-3B C(17)-methyltransferase (EC 2.1.1.131), at 1,886,956 to 1,887,738
GFF1857
GFF1858 - Precorrin-2 C(20)-methyltransferase (EC 2.1.1.130), at 1,887,735 to 1,888,457
GFF1858
GFF1859 - Precorrin-8X methylmutase (EC 5.4.99.61), at 1,888,454 to 1,889,083
GFF1859
GFF1860 - Precorrin-3B synthase, at 1,889,083 to 1,890,393
GFF1860
Position (kb)
1888
1889
1890 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1887.455 kb on - strand, within GFF1857 at 1887.498 kb on + strand, within GFF1857 at 1887.536 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.541 kb on + strand, within GFF1857 at 1887.546 kb on + strand, within GFF1857 at 1887.549 kb on - strand, within GFF1857 at 1887.589 kb on - strand, within GFF1857 at 1887.605 kb on + strand, within GFF1857 at 1887.613 kb on - strand, within GFF1857 at 1887.620 kb on - strand, within GFF1857 at 1887.639 kb on - strand, within GFF1857 at 1887.645 kb on + strand, within GFF1857 at 1887.658 kb on - strand, within GFF1857 at 1887.658 kb on - strand, within GFF1857 at 1887.667 kb on + strand at 1887.724 kb on - strand at 1887.733 kb on + strand at 1887.735 kb on - strand at 1887.754 kb on + strand at 1887.790 kb on - strand at 1887.821 kb on + strand, within GFF1858 at 1887.886 kb on - strand, within GFF1858 at 1887.965 kb on - strand, within GFF1858 at 1887.982 kb on - strand, within GFF1858 at 1888.031 kb on + strand, within GFF1858 at 1888.076 kb on - strand, within GFF1858 at 1888.109 kb on - strand, within GFF1858 at 1888.138 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.212 kb on - strand, within GFF1858 at 1888.311 kb on - strand, within GFF1858 at 1888.316 kb on - strand, within GFF1858 at 1888.418 kb on - strand at 1888.430 kb on + strand at 1888.447 kb on + strand at 1888.511 kb on - strand at 1888.532 kb on + strand, within GFF1859 at 1888.544 kb on - strand, within GFF1859 at 1888.551 kb on + strand, within GFF1859 at 1888.571 kb on - strand, within GFF1859 at 1888.628 kb on + strand, within GFF1859 at 1888.654 kb on + strand, within GFF1859 at 1888.661 kb on - strand, within GFF1859 at 1888.749 kb on + strand, within GFF1859 at 1888.749 kb on - strand, within GFF1859 at 1888.751 kb on - strand, within GFF1859 at 1888.757 kb on - strand, within GFF1859 at 1888.776 kb on + strand, within GFF1859 at 1888.781 kb on + strand, within GFF1859 at 1888.782 kb on - strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.822 kb on - strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.838 kb on + strand, within GFF1859 at 1888.857 kb on + strand, within GFF1859 at 1888.888 kb on - strand, within GFF1859 at 1889.089 kb on - strand at 1889.090 kb on + strand at 1889.119 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.137 kb on - strand at 1889.148 kb on - strand at 1889.163 kb on - strand at 1889.178 kb on - strand at 1889.199 kb on - strand at 1889.230 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.237 kb on - strand, within GFF1860 at 1889.247 kb on + strand, within GFF1860 at 1889.255 kb on - strand, within GFF1860 at 1889.298 kb on + strand, within GFF1860 at 1889.311 kb on - strand, within GFF1860 at 1889.338 kb on + strand, within GFF1860 at 1889.480 kb on + strand, within GFF1860 at 1889.480 kb on + strand, within GFF1860 at 1889.486 kb on - strand, within GFF1860 at 1889.487 kb on - strand, within GFF1860 at 1889.487 kb on - strand, within GFF1860 at 1889.527 kb on + strand, within GFF1860 at 1889.543 kb on + strand, within GFF1860 at 1889.543 kb on + strand, within GFF1860 at 1889.546 kb on - strand, within GFF1860 at 1889.606 kb on + strand, within GFF1860 at 1889.612 kb on - strand, within GFF1860 at 1889.673 kb on - strand, within GFF1860 at 1889.678 kb on + strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.761 kb on + strand, within GFF1860 at 1889.785 kb on + strand, within GFF1860 at 1889.817 kb on - strand, within GFF1860 at 1889.846 kb on - strand, within GFF1860 at 1889.860 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.914 kb on - strand, within GFF1860 at 1889.914 kb on - strand, within GFF1860 at 1889.929 kb on + strand, within GFF1860 at 1889.932 kb on - strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.949 kb on - strand, within GFF1860 at 1889.982 kb on - strand, within GFF1860 at 1889.992 kb on + strand, within GFF1860
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,887,455 - GFF1857 0.64 -0.1 1,887,498 + GFF1857 0.69 -2.3 1,887,536 + GFF1857 0.74 -1.9 1,887,541 + GFF1857 0.75 -1.2 1,887,541 + GFF1857 0.75 -0.7 1,887,541 + GFF1857 0.75 -0.7 1,887,541 + GFF1857 0.75 -1.2 1,887,546 + GFF1857 0.75 -1.0 1,887,549 - GFF1857 0.76 -1.3 1,887,589 - GFF1857 0.81 -1.1 1,887,605 + GFF1857 0.83 +0.6 1,887,613 - GFF1857 0.84 -1.2 1,887,620 - GFF1857 0.85 +0.4 1,887,639 - GFF1857 0.87 -0.7 1,887,645 + GFF1857 0.88 -0.7 1,887,658 - GFF1857 0.90 -1.4 1,887,658 - GFF1857 0.90 -0.9 1,887,667 + -2.1 1,887,724 - -1.3 1,887,733 + -1.7 1,887,735 - -1.5 1,887,754 + -1.1 1,887,790 - -1.7 1,887,821 + GFF1858 0.12 -1.5 1,887,886 - GFF1858 0.21 -0.7 1,887,965 - GFF1858 0.32 -1.3 1,887,982 - GFF1858 0.34 -0.5 1,888,031 + GFF1858 0.41 -0.3 1,888,076 - GFF1858 0.47 -0.9 1,888,109 - GFF1858 0.52 +0.0 1,888,138 + GFF1858 0.56 -2.0 1,888,146 + GFF1858 0.57 -1.0 1,888,146 + GFF1858 0.57 -1.8 1,888,212 - GFF1858 0.66 -1.5 1,888,311 - GFF1858 0.80 -0.7 1,888,316 - GFF1858 0.80 -1.7 1,888,418 - -0.6 1,888,430 + -2.5 1,888,447 + -1.4 1,888,511 - -1.7 1,888,532 + GFF1859 0.12 -0.6 1,888,544 - GFF1859 0.14 +0.6 1,888,551 + GFF1859 0.15 -0.7 1,888,571 - GFF1859 0.19 -2.5 1,888,628 + GFF1859 0.28 -1.1 1,888,654 + GFF1859 0.32 +0.0 1,888,661 - GFF1859 0.33 +0.0 1,888,749 + GFF1859 0.47 -1.5 1,888,749 - GFF1859 0.47 -1.0 1,888,751 - GFF1859 0.47 -1.5 1,888,757 - GFF1859 0.48 -0.9 1,888,776 + GFF1859 0.51 -1.2 1,888,781 + GFF1859 0.52 +0.3 1,888,782 - GFF1859 0.52 -0.6 1,888,791 + GFF1859 0.53 +0.8 1,888,791 + GFF1859 0.53 -2.1 1,888,799 - GFF1859 0.55 -0.2 1,888,799 - GFF1859 0.55 -0.7 1,888,799 - GFF1859 0.55 -0.6 1,888,799 - GFF1859 0.55 +0.3 1,888,822 - GFF1859 0.58 +0.6 1,888,827 + GFF1859 0.59 -2.3 1,888,827 + GFF1859 0.59 -0.9 1,888,838 + GFF1859 0.61 +0.6 1,888,857 + GFF1859 0.64 -2.5 1,888,888 - GFF1859 0.69 +0.6 1,889,089 - +1.9 1,889,090 + -2.6 1,889,119 + -2.1 1,889,124 + -1.0 1,889,124 + -2.1 1,889,124 + -0.9 1,889,124 + -0.9 1,889,124 + +0.3 1,889,124 + -1.7 1,889,129 + -1.7 1,889,129 + -1.8 1,889,129 + +0.3 1,889,132 - -1.1 1,889,132 - +1.4 1,889,132 - -0.9 1,889,132 - -0.3 1,889,132 - -2.6 1,889,137 - +0.7 1,889,148 - -0.7 1,889,163 - -1.0 1,889,178 - -1.0 1,889,199 - -1.3 1,889,230 - GFF1860 0.11 +0.0 1,889,235 - GFF1860 0.12 -1.2 1,889,235 - GFF1860 0.12 +0.3 1,889,237 - GFF1860 0.12 +0.3 1,889,247 + GFF1860 0.13 -0.2 1,889,255 - GFF1860 0.13 +0.3 1,889,298 + GFF1860 0.16 -2.2 1,889,311 - GFF1860 0.17 +0.8 1,889,338 + GFF1860 0.19 -0.4 1,889,480 + GFF1860 0.30 -1.2 1,889,480 + GFF1860 0.30 -1.1 1,889,486 - GFF1860 0.31 +0.0 1,889,487 - GFF1860 0.31 -1.8 1,889,487 - GFF1860 0.31 +0.1 1,889,527 + GFF1860 0.34 -1.6 1,889,543 + GFF1860 0.35 -1.8 1,889,543 + GFF1860 0.35 -1.5 1,889,546 - GFF1860 0.35 -1.8 1,889,606 + GFF1860 0.40 -0.2 1,889,612 - GFF1860 0.40 -0.9 1,889,673 - GFF1860 0.45 -2.0 1,889,678 + GFF1860 0.45 -1.9 1,889,707 - GFF1860 0.48 -1.6 1,889,707 - GFF1860 0.48 +0.6 1,889,707 - GFF1860 0.48 -1.3 1,889,761 + GFF1860 0.52 +0.3 1,889,785 + GFF1860 0.54 -0.6 1,889,817 - GFF1860 0.56 -1.5 1,889,846 - GFF1860 0.58 +0.4 1,889,860 - GFF1860 0.59 -2.1 1,889,868 - GFF1860 0.60 -0.4 1,889,868 - GFF1860 0.60 -0.7 1,889,868 - GFF1860 0.60 +0.0 1,889,914 - GFF1860 0.63 +1.8 1,889,914 - GFF1860 0.63 -2.2 1,889,929 + GFF1860 0.65 -2.1 1,889,932 - GFF1860 0.65 -0.6 1,889,934 + GFF1860 0.65 -0.6 1,889,934 + GFF1860 0.65 -2.3 1,889,934 + GFF1860 0.65 +0.0 1,889,934 + GFF1860 0.65 -1.7 1,889,934 + GFF1860 0.65 -1.3 1,889,934 + GFF1860 0.65 -1.4 1,889,934 + GFF1860 0.65 -1.0 1,889,934 + GFF1860 0.65 +0.3 1,889,934 + GFF1860 0.65 -1.0 1,889,934 + GFF1860 0.65 -0.9 1,889,934 + GFF1860 0.65 -0.6 1,889,942 - GFF1860 0.66 -2.1 1,889,942 - GFF1860 0.66 -1.7 1,889,942 - GFF1860 0.66 -0.1 1,889,942 - GFF1860 0.66 -1.4 1,889,942 - GFF1860 0.66 -2.0 1,889,949 - GFF1860 0.66 -0.9 1,889,982 - GFF1860 0.69 -1.7 1,889,992 + GFF1860 0.69 -0.4
Or see this region's nucleotide sequence