Strain Fitness in Sphingobium sp. HT1-2 around GFF1854

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1852 and GFF1853 are separated by 2 nucleotidesGFF1853 and GFF1854 are separated by 2 nucleotidesGFF1854 and GFF1855 overlap by 4 nucleotides GFF1852 - Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing) (EC 6.3.5.9), at 1,882,582 to 1,883,880 GFF1852 GFF1853 - Precorrin-4 C(11)-methyltransferase (EC 2.1.1.133), at 1,883,883 to 1,884,668 GFF1853 GFF1854 - Cobalamin biosynthesis protein CobE, at 1,884,671 to 1,885,027 GFF1854 GFF1855 - Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (EC 2.1.1.132), at 1,885,024 to 1,886,175 GFF1855 Position (kb) 1884 1885 1886Strain fitness (log2 ratio) -3 -2 -1 0 1at 1883.671 kb on + strand, within GFF1852at 1883.671 kb on + strand, within GFF1852at 1883.679 kb on - strand, within GFF1852at 1883.679 kb on - strand, within GFF1852at 1883.679 kb on - strand, within GFF1852at 1883.688 kb on + strand, within GFF1852at 1883.706 kb on + strand, within GFF1852at 1883.748 kb on + strand, within GFF1852at 1883.753 kb on + strandat 1883.785 kb on + strandat 1883.789 kb on - strandat 1883.798 kb on - strandat 1883.807 kb on + strandat 1883.894 kb on + strandat 1883.894 kb on + strandat 1883.902 kb on - strandat 1883.907 kb on - strandat 1883.921 kb on - strandat 1883.937 kb on + strandat 1883.938 kb on + strandat 1883.938 kb on + strandat 1883.938 kb on + strandat 1883.944 kb on - strandat 1883.946 kb on - strandat 1884.017 kb on + strand, within GFF1853at 1884.017 kb on + strand, within GFF1853at 1884.017 kb on + strand, within GFF1853at 1884.017 kb on + strand, within GFF1853at 1884.025 kb on - strand, within GFF1853at 1884.025 kb on - strand, within GFF1853at 1884.025 kb on - strand, within GFF1853at 1884.025 kb on - strand, within GFF1853at 1884.040 kb on + strand, within GFF1853at 1884.077 kb on + strand, within GFF1853at 1884.085 kb on - strand, within GFF1853at 1884.112 kb on + strand, within GFF1853at 1884.145 kb on + strand, within GFF1853at 1884.145 kb on + strand, within GFF1853at 1884.145 kb on + strand, within GFF1853at 1884.153 kb on - strand, within GFF1853at 1884.183 kb on + strand, within GFF1853at 1884.183 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.238 kb on + strand, within GFF1853at 1884.246 kb on - strand, within GFF1853at 1884.246 kb on - strand, within GFF1853at 1884.246 kb on - strand, within GFF1853at 1884.248 kb on + strand, within GFF1853at 1884.283 kb on + strand, within GFF1853at 1884.283 kb on + strand, within GFF1853at 1884.284 kb on + strand, within GFF1853at 1884.314 kb on - strand, within GFF1853at 1884.437 kb on + strand, within GFF1853at 1884.439 kb on + strand, within GFF1853at 1884.487 kb on + strand, within GFF1853at 1884.494 kb on + strand, within GFF1853at 1884.508 kb on + strand, within GFF1853at 1884.516 kb on - strand, within GFF1853at 1884.541 kb on - strand, within GFF1853at 1884.579 kb on - strand, within GFF1853at 1884.677 kb on + strandat 1884.678 kb on + strandat 1884.678 kb on - strandat 1884.719 kb on + strand, within GFF1854at 1884.727 kb on - strand, within GFF1854at 1884.765 kb on + strand, within GFF1854at 1884.771 kb on - strand, within GFF1854at 1884.771 kb on - strand, within GFF1854at 1884.784 kb on + strand, within GFF1854at 1884.828 kb on - strand, within GFF1854at 1884.838 kb on - strand, within GFF1854at 1884.911 kb on - strand, within GFF1854at 1884.979 kb on + strand, within GFF1854at 1885.034 kb on + strandat 1885.037 kb on - strandat 1885.039 kb on + strandat 1885.102 kb on - strandat 1885.102 kb on - strandat 1885.113 kb on + strandat 1885.113 kb on + strandat 1885.142 kb on - strand, within GFF1855at 1885.164 kb on + strand, within GFF1855at 1885.191 kb on + strand, within GFF1855at 1885.199 kb on - strand, within GFF1855at 1885.235 kb on + strand, within GFF1855at 1885.255 kb on + strand, within GFF1855at 1885.317 kb on + strand, within GFF1855at 1885.346 kb on - strand, within GFF1855at 1885.357 kb on + strand, within GFF1855at 1885.357 kb on + strand, within GFF1855at 1885.372 kb on + strand, within GFF1855at 1885.372 kb on + strand, within GFF1855at 1885.376 kb on + strand, within GFF1855at 1885.380 kb on - strand, within GFF1855at 1885.381 kb on + strand, within GFF1855at 1885.389 kb on - strand, within GFF1855at 1885.389 kb on - strand, within GFF1855at 1885.404 kb on - strand, within GFF1855at 1885.467 kb on - strand, within GFF1855at 1885.500 kb on + strand, within GFF1855at 1885.508 kb on - strand, within GFF1855at 1885.508 kb on - strand, within GFF1855at 1885.508 kb on - strand, within GFF1855at 1885.576 kb on + strand, within GFF1855at 1885.576 kb on + strand, within GFF1855at 1885.576 kb on + strand, within GFF1855at 1885.576 kb on + strand, within GFF1855at 1885.576 kb on + strand, within GFF1855at 1885.581 kb on + strand, within GFF1855at 1885.581 kb on + strand, within GFF1855at 1885.581 kb on + strand, within GFF1855at 1885.584 kb on - strand, within GFF1855at 1885.584 kb on - strand, within GFF1855at 1885.589 kb on - strand, within GFF1855at 1885.589 kb on - strand, within GFF1855at 1885.589 kb on - strand, within GFF1855at 1885.589 kb on - strand, within GFF1855at 1885.602 kb on + strand, within GFF1855at 1885.602 kb on + strand, within GFF1855at 1885.602 kb on + strand, within GFF1855at 1885.602 kb on + strand, within GFF1855at 1885.610 kb on - strand, within GFF1855at 1885.610 kb on - strand, within GFF1855at 1885.610 kb on - strand, within GFF1855at 1885.610 kb on - strand, within GFF1855at 1885.694 kb on - strand, within GFF1855at 1885.823 kb on + strand, within GFF1855at 1885.866 kb on + strand, within GFF1855at 1885.911 kb on - strand, within GFF1855at 1885.935 kb on - strand, within GFF1855at 1885.957 kb on + strand, within GFF1855at 1885.997 kb on + strand, within GFF1855at 1886.013 kb on + strand, within GFF1855

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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1,883,671 + GFF1852 0.84 -1.2
1,883,671 + GFF1852 0.84 -0.9
1,883,679 - GFF1852 0.84 -1.5
1,883,679 - GFF1852 0.84 -2.4
1,883,679 - GFF1852 0.84 +0.8
1,883,688 + GFF1852 0.85 -0.9
1,883,706 + GFF1852 0.87 -1.7
1,883,748 + GFF1852 0.90 -0.9
1,883,753 + -1.5
1,883,785 + -1.1
1,883,789 - -1.5
1,883,798 - +0.4
1,883,807 + +0.3
1,883,894 + +0.8
1,883,894 + +1.0
1,883,902 - -0.8
1,883,907 - +1.4
1,883,921 - -0.1
1,883,937 + +1.3
1,883,938 + -1.0
1,883,938 + +0.6
1,883,938 + -0.4
1,883,944 - +0.6
1,883,946 - -1.7
1,884,017 + GFF1853 0.17 -2.3
1,884,017 + GFF1853 0.17 +0.3
1,884,017 + GFF1853 0.17 -3.1
1,884,017 + GFF1853 0.17 -0.2
1,884,025 - GFF1853 0.18 -0.7
1,884,025 - GFF1853 0.18 -1.8
1,884,025 - GFF1853 0.18 -0.7
1,884,025 - GFF1853 0.18 -1.3
1,884,040 + GFF1853 0.20 -1.3
1,884,077 + GFF1853 0.25 +0.3
1,884,085 - GFF1853 0.26 -1.1
1,884,112 + GFF1853 0.29 +0.6
1,884,145 + GFF1853 0.33 -0.4
1,884,145 + GFF1853 0.33 -0.4
1,884,145 + GFF1853 0.33 +0.6
1,884,153 - GFF1853 0.34 -1.1
1,884,183 + GFF1853 0.38 -1.0
1,884,183 + GFF1853 0.38 -0.2
1,884,238 + GFF1853 0.45 -0.4
1,884,238 + GFF1853 0.45 -1.3
1,884,238 + GFF1853 0.45 -1.7
1,884,238 + GFF1853 0.45 +0.4
1,884,238 + GFF1853 0.45 -1.6
1,884,238 + GFF1853 0.45 -1.6
1,884,246 - GFF1853 0.46 -2.0
1,884,246 - GFF1853 0.46 -2.0
1,884,246 - GFF1853 0.46 -1.2
1,884,248 + GFF1853 0.46 -1.2
1,884,283 + GFF1853 0.51 -0.6
1,884,283 + GFF1853 0.51 -0.3
1,884,284 + GFF1853 0.51 +0.6
1,884,314 - GFF1853 0.55 -1.4
1,884,437 + GFF1853 0.70 -1.7
1,884,439 + GFF1853 0.71 +0.3
1,884,487 + GFF1853 0.77 -1.6
1,884,494 + GFF1853 0.78 -1.1
1,884,508 + GFF1853 0.80 -2.9
1,884,516 - GFF1853 0.81 -1.6
1,884,541 - GFF1853 0.84 -1.8
1,884,579 - GFF1853 0.89 -1.0
1,884,677 + +0.5
1,884,678 + -2.2
1,884,678 - +0.8
1,884,719 + GFF1854 0.13 -0.6
1,884,727 - GFF1854 0.16 -1.9
1,884,765 + GFF1854 0.26 -0.4
1,884,771 - GFF1854 0.28 -2.8
1,884,771 - GFF1854 0.28 -0.6
1,884,784 + GFF1854 0.32 +0.4
1,884,828 - GFF1854 0.44 -1.6
1,884,838 - GFF1854 0.47 -0.6
1,884,911 - GFF1854 0.67 -1.7
1,884,979 + GFF1854 0.86 -0.9
1,885,034 + -2.6
1,885,037 - -1.7
1,885,039 + -1.3
1,885,102 - -2.7
1,885,102 - +0.6
1,885,113 + -2.2
1,885,113 + -2.3
1,885,142 - GFF1855 0.10 -1.5
1,885,164 + GFF1855 0.12 -1.3
1,885,191 + GFF1855 0.14 -0.7
1,885,199 - GFF1855 0.15 -2.1
1,885,235 + GFF1855 0.18 -1.6
1,885,255 + GFF1855 0.20 -0.6
1,885,317 + GFF1855 0.25 -0.7
1,885,346 - GFF1855 0.28 -0.1
1,885,357 + GFF1855 0.29 -0.4
1,885,357 + GFF1855 0.29 -1.0
1,885,372 + GFF1855 0.30 -1.9
1,885,372 + GFF1855 0.30 +0.0
1,885,376 + GFF1855 0.31 -1.4
1,885,380 - GFF1855 0.31 -2.7
1,885,381 + GFF1855 0.31 -0.9
1,885,389 - GFF1855 0.32 +0.6
1,885,389 - GFF1855 0.32 -1.8
1,885,404 - GFF1855 0.33 -0.9
1,885,467 - GFF1855 0.38 -0.4
1,885,500 + GFF1855 0.41 -2.3
1,885,508 - GFF1855 0.42 -1.1
1,885,508 - GFF1855 0.42 -1.0
1,885,508 - GFF1855 0.42 -0.2
1,885,576 + GFF1855 0.48 -0.4
1,885,576 + GFF1855 0.48 -0.9
1,885,576 + GFF1855 0.48 -1.1
1,885,576 + GFF1855 0.48 -2.2
1,885,576 + GFF1855 0.48 -0.9
1,885,581 + GFF1855 0.48 -1.0
1,885,581 + GFF1855 0.48 -1.0
1,885,581 + GFF1855 0.48 +0.3
1,885,584 - GFF1855 0.49 -1.6
1,885,584 - GFF1855 0.49 -1.9
1,885,589 - GFF1855 0.49 -0.2
1,885,589 - GFF1855 0.49 -0.4
1,885,589 - GFF1855 0.49 -0.6
1,885,589 - GFF1855 0.49 -1.8
1,885,602 + GFF1855 0.50 -1.1
1,885,602 + GFF1855 0.50 +0.4
1,885,602 + GFF1855 0.50 -1.7
1,885,602 + GFF1855 0.50 -0.3
1,885,610 - GFF1855 0.51 -0.6
1,885,610 - GFF1855 0.51 -0.9
1,885,610 - GFF1855 0.51 -0.7
1,885,610 - GFF1855 0.51 -1.7
1,885,694 - GFF1855 0.58 -1.6
1,885,823 + GFF1855 0.69 -0.9
1,885,866 + GFF1855 0.73 +1.0
1,885,911 - GFF1855 0.77 -1.9
1,885,935 - GFF1855 0.79 -1.3
1,885,957 + GFF1855 0.81 -1.0
1,885,997 + GFF1855 0.84 -1.0
1,886,013 + GFF1855 0.86 -1.2

Or see this region's nucleotide sequence