Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1852 and GFF1853 are separated by 2 nucleotides GFF1853 and GFF1854 are separated by 2 nucleotides GFF1854 and GFF1855 overlap by 4 nucleotides
GFF1852 - Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing) (EC 6.3.5.9), at 1,882,582 to 1,883,880
GFF1852
GFF1853 - Precorrin-4 C(11)-methyltransferase (EC 2.1.1.133), at 1,883,883 to 1,884,668
GFF1853
GFF1854 - Cobalamin biosynthesis protein CobE, at 1,884,671 to 1,885,027
GFF1854
GFF1855 - Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (EC 2.1.1.132), at 1,885,024 to 1,886,175
GFF1855
Position (kb)
1884
1885
1886 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1883.671 kb on + strand, within GFF1852 at 1883.671 kb on + strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.688 kb on + strand, within GFF1852 at 1883.706 kb on + strand, within GFF1852 at 1883.748 kb on + strand, within GFF1852 at 1883.753 kb on + strand at 1883.785 kb on + strand at 1883.789 kb on - strand at 1883.798 kb on - strand at 1883.807 kb on + strand at 1883.894 kb on + strand at 1883.894 kb on + strand at 1883.902 kb on - strand at 1883.907 kb on - strand at 1883.921 kb on - strand at 1883.937 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.944 kb on - strand at 1883.946 kb on - strand at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.040 kb on + strand, within GFF1853 at 1884.077 kb on + strand, within GFF1853 at 1884.085 kb on - strand, within GFF1853 at 1884.112 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.153 kb on - strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.248 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.284 kb on + strand, within GFF1853 at 1884.314 kb on - strand, within GFF1853 at 1884.437 kb on + strand, within GFF1853 at 1884.439 kb on + strand, within GFF1853 at 1884.487 kb on + strand, within GFF1853 at 1884.494 kb on + strand, within GFF1853 at 1884.508 kb on + strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.541 kb on - strand, within GFF1853 at 1884.579 kb on - strand, within GFF1853 at 1884.677 kb on + strand at 1884.678 kb on + strand at 1884.678 kb on - strand at 1884.719 kb on + strand, within GFF1854 at 1884.727 kb on - strand, within GFF1854 at 1884.765 kb on + strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.784 kb on + strand, within GFF1854 at 1884.828 kb on - strand, within GFF1854 at 1884.838 kb on - strand, within GFF1854 at 1884.911 kb on - strand, within GFF1854 at 1884.979 kb on + strand, within GFF1854 at 1885.034 kb on + strand at 1885.037 kb on - strand at 1885.039 kb on + strand at 1885.102 kb on - strand at 1885.102 kb on - strand at 1885.113 kb on + strand at 1885.113 kb on + strand at 1885.142 kb on - strand, within GFF1855 at 1885.164 kb on + strand, within GFF1855 at 1885.191 kb on + strand, within GFF1855 at 1885.199 kb on - strand, within GFF1855 at 1885.235 kb on + strand, within GFF1855 at 1885.255 kb on + strand, within GFF1855 at 1885.317 kb on + strand, within GFF1855 at 1885.346 kb on - strand, within GFF1855 at 1885.357 kb on + strand, within GFF1855 at 1885.357 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.376 kb on + strand, within GFF1855 at 1885.380 kb on - strand, within GFF1855 at 1885.381 kb on + strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.404 kb on - strand, within GFF1855 at 1885.467 kb on - strand, within GFF1855 at 1885.500 kb on + strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.694 kb on - strand, within GFF1855 at 1885.823 kb on + strand, within GFF1855 at 1885.866 kb on + strand, within GFF1855 at 1885.911 kb on - strand, within GFF1855 at 1885.935 kb on - strand, within GFF1855 at 1885.957 kb on + strand, within GFF1855 at 1885.997 kb on + strand, within GFF1855 at 1886.013 kb on + strand, within GFF1855
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,883,671 + GFF1852 0.84 -1.2 1,883,671 + GFF1852 0.84 -0.9 1,883,679 - GFF1852 0.84 -1.5 1,883,679 - GFF1852 0.84 -2.4 1,883,679 - GFF1852 0.84 +0.8 1,883,688 + GFF1852 0.85 -0.9 1,883,706 + GFF1852 0.87 -1.7 1,883,748 + GFF1852 0.90 -0.9 1,883,753 + -1.5 1,883,785 + -1.1 1,883,789 - -1.5 1,883,798 - +0.4 1,883,807 + +0.3 1,883,894 + +0.8 1,883,894 + +1.0 1,883,902 - -0.8 1,883,907 - +1.4 1,883,921 - -0.1 1,883,937 + +1.3 1,883,938 + -1.0 1,883,938 + +0.6 1,883,938 + -0.4 1,883,944 - +0.6 1,883,946 - -1.7 1,884,017 + GFF1853 0.17 -2.3 1,884,017 + GFF1853 0.17 +0.3 1,884,017 + GFF1853 0.17 -3.1 1,884,017 + GFF1853 0.17 -0.2 1,884,025 - GFF1853 0.18 -0.7 1,884,025 - GFF1853 0.18 -1.8 1,884,025 - GFF1853 0.18 -0.7 1,884,025 - GFF1853 0.18 -1.3 1,884,040 + GFF1853 0.20 -1.3 1,884,077 + GFF1853 0.25 +0.3 1,884,085 - GFF1853 0.26 -1.1 1,884,112 + GFF1853 0.29 +0.6 1,884,145 + GFF1853 0.33 -0.4 1,884,145 + GFF1853 0.33 -0.4 1,884,145 + GFF1853 0.33 +0.6 1,884,153 - GFF1853 0.34 -1.1 1,884,183 + GFF1853 0.38 -1.0 1,884,183 + GFF1853 0.38 -0.2 1,884,238 + GFF1853 0.45 -0.4 1,884,238 + GFF1853 0.45 -1.3 1,884,238 + GFF1853 0.45 -1.7 1,884,238 + GFF1853 0.45 +0.4 1,884,238 + GFF1853 0.45 -1.6 1,884,238 + GFF1853 0.45 -1.6 1,884,246 - GFF1853 0.46 -2.0 1,884,246 - GFF1853 0.46 -2.0 1,884,246 - GFF1853 0.46 -1.2 1,884,248 + GFF1853 0.46 -1.2 1,884,283 + GFF1853 0.51 -0.6 1,884,283 + GFF1853 0.51 -0.3 1,884,284 + GFF1853 0.51 +0.6 1,884,314 - GFF1853 0.55 -1.4 1,884,437 + GFF1853 0.70 -1.7 1,884,439 + GFF1853 0.71 +0.3 1,884,487 + GFF1853 0.77 -1.6 1,884,494 + GFF1853 0.78 -1.1 1,884,508 + GFF1853 0.80 -2.9 1,884,516 - GFF1853 0.81 -1.6 1,884,541 - GFF1853 0.84 -1.8 1,884,579 - GFF1853 0.89 -1.0 1,884,677 + +0.5 1,884,678 + -2.2 1,884,678 - +0.8 1,884,719 + GFF1854 0.13 -0.6 1,884,727 - GFF1854 0.16 -1.9 1,884,765 + GFF1854 0.26 -0.4 1,884,771 - GFF1854 0.28 -2.8 1,884,771 - GFF1854 0.28 -0.6 1,884,784 + GFF1854 0.32 +0.4 1,884,828 - GFF1854 0.44 -1.6 1,884,838 - GFF1854 0.47 -0.6 1,884,911 - GFF1854 0.67 -1.7 1,884,979 + GFF1854 0.86 -0.9 1,885,034 + -2.6 1,885,037 - -1.7 1,885,039 + -1.3 1,885,102 - -2.7 1,885,102 - +0.6 1,885,113 + -2.2 1,885,113 + -2.3 1,885,142 - GFF1855 0.10 -1.5 1,885,164 + GFF1855 0.12 -1.3 1,885,191 + GFF1855 0.14 -0.7 1,885,199 - GFF1855 0.15 -2.1 1,885,235 + GFF1855 0.18 -1.6 1,885,255 + GFF1855 0.20 -0.6 1,885,317 + GFF1855 0.25 -0.7 1,885,346 - GFF1855 0.28 -0.1 1,885,357 + GFF1855 0.29 -0.4 1,885,357 + GFF1855 0.29 -1.0 1,885,372 + GFF1855 0.30 -1.9 1,885,372 + GFF1855 0.30 +0.0 1,885,376 + GFF1855 0.31 -1.4 1,885,380 - GFF1855 0.31 -2.7 1,885,381 + GFF1855 0.31 -0.9 1,885,389 - GFF1855 0.32 +0.6 1,885,389 - GFF1855 0.32 -1.8 1,885,404 - GFF1855 0.33 -0.9 1,885,467 - GFF1855 0.38 -0.4 1,885,500 + GFF1855 0.41 -2.3 1,885,508 - GFF1855 0.42 -1.1 1,885,508 - GFF1855 0.42 -1.0 1,885,508 - GFF1855 0.42 -0.2 1,885,576 + GFF1855 0.48 -0.4 1,885,576 + GFF1855 0.48 -0.9 1,885,576 + GFF1855 0.48 -1.1 1,885,576 + GFF1855 0.48 -2.2 1,885,576 + GFF1855 0.48 -0.9 1,885,581 + GFF1855 0.48 -1.0 1,885,581 + GFF1855 0.48 -1.0 1,885,581 + GFF1855 0.48 +0.3 1,885,584 - GFF1855 0.49 -1.6 1,885,584 - GFF1855 0.49 -1.9 1,885,589 - GFF1855 0.49 -0.2 1,885,589 - GFF1855 0.49 -0.4 1,885,589 - GFF1855 0.49 -0.6 1,885,589 - GFF1855 0.49 -1.8 1,885,602 + GFF1855 0.50 -1.1 1,885,602 + GFF1855 0.50 +0.4 1,885,602 + GFF1855 0.50 -1.7 1,885,602 + GFF1855 0.50 -0.3 1,885,610 - GFF1855 0.51 -0.6 1,885,610 - GFF1855 0.51 -0.9 1,885,610 - GFF1855 0.51 -0.7 1,885,610 - GFF1855 0.51 -1.7 1,885,694 - GFF1855 0.58 -1.6 1,885,823 + GFF1855 0.69 -0.9 1,885,866 + GFF1855 0.73 +1.0 1,885,911 - GFF1855 0.77 -1.9 1,885,935 - GFF1855 0.79 -1.3 1,885,957 + GFF1855 0.81 -1.0 1,885,997 + GFF1855 0.84 -1.0 1,886,013 + GFF1855 0.86 -1.2
Or see this region's nucleotide sequence