Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF130 and GFF131 are separated by 49 nucleotides GFF131 and GFF132 are separated by 167 nucleotides GFF132 and GFF133 are separated by 0 nucleotides
GFF130 - Excinuclease ABC, C subunit-like, at 124,850 to 125,140
GFF130
GFF131 - Methylmalonyl-CoA epimerase (EC 5.1.99.1) @ Ethylmalonyl-CoA epimerase, at 125,190 to 125,630
GFF131
GFF132 - Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2), at 125,798 to 127,951
GFF132
GFF133 - Biotin synthase (EC 2.8.1.6), at 127,952 to 128,965
GFF133
Position (kb)
125
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 124.825 kb on + strand at 124.830 kb on + strand at 124.830 kb on + strand at 124.840 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.862 kb on + strand at 124.894 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.895 kb on + strand, within GFF130 at 124.897 kb on + strand, within GFF130 at 124.899 kb on + strand, within GFF130 at 124.899 kb on + strand, within GFF130 at 124.902 kb on - strand, within GFF130 at 124.902 kb on - strand, within GFF130 at 124.905 kb on - strand, within GFF130 at 124.910 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.911 kb on + strand, within GFF130 at 124.912 kb on - strand, within GFF130 at 124.929 kb on + strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.956 kb on - strand, within GFF130 at 124.996 kb on - strand, within GFF130 at 124.996 kb on - strand, within GFF130 at 124.998 kb on + strand, within GFF130 at 125.006 kb on - strand, within GFF130 at 125.016 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.047 kb on + strand, within GFF130 at 125.050 kb on - strand, within GFF130 at 125.055 kb on - strand, within GFF130 at 125.060 kb on - strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.092 kb on + strand, within GFF130 at 125.100 kb on - strand, within GFF130 at 125.137 kb on - strand at 125.137 kb on - strand at 125.143 kb on - strand at 125.151 kb on + strand at 125.181 kb on + strand at 125.211 kb on - strand at 125.284 kb on + strand, within GFF131 at 125.292 kb on - strand, within GFF131 at 125.329 kb on - strand, within GFF131 at 125.351 kb on - strand, within GFF131 at 125.391 kb on + strand, within GFF131 at 125.391 kb on + strand, within GFF131 at 125.430 kb on - strand, within GFF131 at 125.453 kb on - strand, within GFF131 at 125.455 kb on + strand, within GFF131 at 125.463 kb on - strand, within GFF131 at 125.492 kb on + strand, within GFF131 at 125.575 kb on - strand, within GFF131 at 125.575 kb on - strand, within GFF131 at 125.576 kb on + strand, within GFF131 at 125.584 kb on - strand, within GFF131 at 125.589 kb on - strand at 125.606 kb on - strand at 125.633 kb on - strand at 125.637 kb on - strand at 125.701 kb on + strand at 125.706 kb on + strand at 125.706 kb on + strand at 125.779 kb on + strand at 125.779 kb on + strand at 125.786 kb on + strand at 125.790 kb on + strand at 125.861 kb on + strand at 125.902 kb on + strand at 125.902 kb on + strand at 125.921 kb on + strand at 126.003 kb on - strand at 126.003 kb on - strand at 126.074 kb on + strand, within GFF132 at 126.191 kb on - strand, within GFF132 at 126.338 kb on - strand, within GFF132 at 126.348 kb on + strand, within GFF132 at 126.375 kb on - strand, within GFF132 at 126.729 kb on + strand, within GFF132 at 126.833 kb on - strand, within GFF132 at 126.945 kb on - strand, within GFF132 at 127.056 kb on - strand, within GFF132 at 127.067 kb on + strand, within GFF132 at 127.203 kb on + strand, within GFF132 at 127.243 kb on + strand, within GFF132 at 127.278 kb on - strand, within GFF132 at 127.288 kb on - strand, within GFF132 at 127.342 kb on - strand, within GFF132 at 127.405 kb on - strand, within GFF132 at 127.454 kb on + strand, within GFF132 at 127.468 kb on - strand, within GFF132 at 127.596 kb on - strand, within GFF132 at 127.650 kb on - strand, within GFF132 at 127.745 kb on - strand at 127.874 kb on + strand at 127.915 kb on + strand at 127.919 kb on - strand at 127.923 kb on - strand at 127.923 kb on - strand at 127.923 kb on - strand at 127.931 kb on + strand at 127.936 kb on + strand at 127.939 kb on - strand at 127.946 kb on + strand at 127.960 kb on + strand at 127.984 kb on + strand at 127.985 kb on + strand at 128.062 kb on - strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.092 kb on + strand, within GFF133 at 128.100 kb on + strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.100 kb on - strand, within GFF133 at 128.103 kb on - strand, within GFF133 at 128.127 kb on + strand, within GFF133 at 128.134 kb on + strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.135 kb on - strand, within GFF133 at 128.148 kb on + strand, within GFF133 at 128.161 kb on - strand, within GFF133 at 128.191 kb on + strand, within GFF133 at 128.207 kb on - strand, within GFF133 at 128.218 kb on - strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.230 kb on + strand, within GFF133 at 128.237 kb on + strand, within GFF133 at 128.247 kb on + strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.278 kb on - strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.280 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.317 kb on + strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.325 kb on - strand, within GFF133 at 128.371 kb on - strand, within GFF133 at 128.430 kb on - strand, within GFF133 at 128.486 kb on + strand, within GFF133 at 128.486 kb on + strand, within GFF133 at 128.494 kb on - strand, within GFF133 at 128.555 kb on + strand, within GFF133 at 128.563 kb on - strand, within GFF133 at 128.572 kb on - strand, within GFF133 at 128.596 kb on + strand, within GFF133 at 128.600 kb on + strand, within GFF133 at 128.612 kb on + strand, within GFF133 at 128.615 kb on + strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.620 kb on - strand, within GFF133 at 128.632 kb on - strand, within GFF133 at 128.655 kb on + strand, within GFF133 at 128.655 kb on - strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.660 kb on + strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.668 kb on - strand, within GFF133 at 128.711 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.724 kb on + strand, within GFF133 at 128.742 kb on - strand, within GFF133 at 128.757 kb on + strand, within GFF133 at 128.762 kb on + strand, within GFF133 at 128.765 kb on - strand, within GFF133 at 128.767 kb on + strand, within GFF133 at 128.770 kb on - strand, within GFF133 at 128.770 kb on - strand, within GFF133 at 128.855 kb on + strand, within GFF133 at 128.873 kb on + strand at 128.908 kb on + strand at 128.908 kb on + strand at 128.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 124,825 + -0.7 124,830 + +1.3 124,830 + -0.4 124,840 + -2.1 124,862 + -0.5 124,862 + -0.8 124,862 + +0.6 124,862 + +0.1 124,862 + -0.9 124,894 + GFF130 0.15 -1.0 124,895 + GFF130 0.15 -0.1 124,895 + GFF130 0.15 +1.0 124,895 + GFF130 0.15 +1.3 124,897 + GFF130 0.16 +0.2 124,899 + GFF130 0.17 -0.5 124,899 + GFF130 0.17 -0.7 124,902 - GFF130 0.18 -1.4 124,902 - GFF130 0.18 -0.7 124,905 - GFF130 0.19 -0.0 124,910 + GFF130 0.21 -0.0 124,911 + GFF130 0.21 +0.3 124,911 + GFF130 0.21 -1.7 124,911 + GFF130 0.21 +0.6 124,911 + GFF130 0.21 +0.3 124,912 - GFF130 0.21 -0.3 124,929 + GFF130 0.27 +0.2 124,956 - GFF130 0.36 +1.0 124,956 - GFF130 0.36 -2.0 124,956 - GFF130 0.36 -0.9 124,996 - GFF130 0.50 -0.4 124,996 - GFF130 0.50 -0.2 124,998 + GFF130 0.51 -0.2 125,006 - GFF130 0.54 -0.8 125,016 + GFF130 0.57 -0.1 125,047 + GFF130 0.68 +2.1 125,047 + GFF130 0.68 +0.6 125,047 + GFF130 0.68 +0.4 125,047 + GFF130 0.68 -0.3 125,047 + GFF130 0.68 -0.2 125,050 - GFF130 0.69 -1.3 125,055 - GFF130 0.70 +0.2 125,060 - GFF130 0.72 -0.7 125,092 + GFF130 0.83 +0.3 125,092 + GFF130 0.83 -0.4 125,092 + GFF130 0.83 -0.4 125,100 - GFF130 0.86 -1.7 125,137 - +1.2 125,137 - +0.0 125,143 - -1.5 125,151 + -0.5 125,181 + +0.6 125,211 - -0.3 125,284 + GFF131 0.21 -0.3 125,292 - GFF131 0.23 -0.0 125,329 - GFF131 0.32 -1.8 125,351 - GFF131 0.37 -1.3 125,391 + GFF131 0.46 -0.0 125,391 + GFF131 0.46 -0.0 125,430 - GFF131 0.54 +0.2 125,453 - GFF131 0.60 -0.4 125,455 + GFF131 0.60 -0.4 125,463 - GFF131 0.62 +0.9 125,492 + GFF131 0.68 -0.6 125,575 - GFF131 0.87 -0.6 125,575 - GFF131 0.87 -0.3 125,576 + GFF131 0.88 -1.6 125,584 - GFF131 0.89 +0.2 125,589 - +0.6 125,606 - -2.1 125,633 - -1.0 125,637 - +1.3 125,701 + -0.3 125,706 + +1.1 125,706 + -0.9 125,779 + +0.7 125,779 + -2.2 125,786 + +0.2 125,790 + +1.6 125,861 + -0.6 125,902 + +0.6 125,902 + -0.0 125,921 + -1.1 126,003 - -0.3 126,003 - +0.2 126,074 + GFF132 0.13 -1.8 126,191 - GFF132 0.18 -0.6 126,338 - GFF132 0.25 +0.7 126,348 + GFF132 0.26 -0.3 126,375 - GFF132 0.27 +0.2 126,729 + GFF132 0.43 +1.4 126,833 - GFF132 0.48 -1.7 126,945 - GFF132 0.53 +0.2 127,056 - GFF132 0.58 +0.6 127,067 + GFF132 0.59 -0.3 127,203 + GFF132 0.65 -0.3 127,243 + GFF132 0.67 -0.4 127,278 - GFF132 0.69 +0.4 127,288 - GFF132 0.69 +0.2 127,342 - GFF132 0.72 -0.3 127,405 - GFF132 0.75 +0.2 127,454 + GFF132 0.77 -0.0 127,468 - GFF132 0.78 +0.2 127,596 - GFF132 0.83 +0.2 127,650 - GFF132 0.86 +0.2 127,745 - +1.2 127,874 + -0.0 127,915 + -0.1 127,919 - +0.0 127,923 - +0.2 127,923 - -0.4 127,923 - -0.4 127,931 + +1.0 127,936 + +0.8 127,939 - +0.4 127,946 + -0.7 127,960 + -0.8 127,984 + +0.6 127,985 + +0.4 128,062 - GFF133 0.11 -0.4 128,092 + GFF133 0.14 +0.1 128,092 + GFF133 0.14 -1.0 128,100 + GFF133 0.15 +0.1 128,100 - GFF133 0.15 +0.7 128,100 - GFF133 0.15 -0.4 128,103 - GFF133 0.15 +0.8 128,127 + GFF133 0.17 +0.7 128,134 + GFF133 0.18 -0.8 128,135 - GFF133 0.18 -1.4 128,135 - GFF133 0.18 +1.3 128,135 - GFF133 0.18 +0.1 128,135 - GFF133 0.18 -1.1 128,148 + GFF133 0.19 -0.9 128,161 - GFF133 0.21 -1.8 128,191 + GFF133 0.24 -0.5 128,207 - GFF133 0.25 -0.1 128,218 - GFF133 0.26 -0.9 128,230 + GFF133 0.27 -0.7 128,230 + GFF133 0.27 -1.2 128,230 + GFF133 0.27 -0.6 128,237 + GFF133 0.28 +0.3 128,247 + GFF133 0.29 +1.6 128,278 - GFF133 0.32 +0.8 128,278 - GFF133 0.32 -1.0 128,280 + GFF133 0.32 -1.3 128,280 + GFF133 0.32 -0.3 128,280 + GFF133 0.32 -0.1 128,317 + GFF133 0.36 -0.3 128,317 + GFF133 0.36 +1.0 128,317 + GFF133 0.36 -0.1 128,317 + GFF133 0.36 -1.2 128,325 - GFF133 0.37 +0.3 128,325 - GFF133 0.37 -0.1 128,325 - GFF133 0.37 -0.8 128,325 - GFF133 0.37 -0.3 128,325 - GFF133 0.37 +0.4 128,371 - GFF133 0.41 -1.7 128,430 - GFF133 0.47 -1.8 128,486 + GFF133 0.53 +0.2 128,486 + GFF133 0.53 -0.3 128,494 - GFF133 0.53 -0.9 128,555 + GFF133 0.59 +0.2 128,563 - GFF133 0.60 -0.7 128,572 - GFF133 0.61 -0.4 128,596 + GFF133 0.64 -0.6 128,600 + GFF133 0.64 -0.0 128,612 + GFF133 0.65 +0.4 128,615 + GFF133 0.65 +0.6 128,620 - GFF133 0.66 +1.1 128,620 - GFF133 0.66 +0.7 128,632 - GFF133 0.67 -0.5 128,655 + GFF133 0.69 -0.0 128,655 - GFF133 0.69 +0.4 128,660 + GFF133 0.70 -0.9 128,660 + GFF133 0.70 -2.1 128,660 + GFF133 0.70 -1.7 128,660 + GFF133 0.70 +1.6 128,660 + GFF133 0.70 -0.6 128,668 - GFF133 0.71 -1.1 128,668 - GFF133 0.71 +0.2 128,668 - GFF133 0.71 +0.0 128,668 - GFF133 0.71 +1.5 128,668 - GFF133 0.71 +0.3 128,668 - GFF133 0.71 -1.8 128,668 - GFF133 0.71 +0.0 128,668 - GFF133 0.71 -0.4 128,711 + GFF133 0.75 +1.4 128,724 + GFF133 0.76 +0.0 128,724 + GFF133 0.76 -1.0 128,742 - GFF133 0.78 +0.3 128,757 + GFF133 0.79 -1.6 128,762 + GFF133 0.80 -0.6 128,765 - GFF133 0.80 -1.1 128,767 + GFF133 0.80 +0.1 128,770 - GFF133 0.81 +1.2 128,770 - GFF133 0.81 -0.0 128,855 + GFF133 0.89 -0.3 128,873 + +0.7 128,908 + +1.8 128,908 + -0.7 128,918 + +1.6
Or see this region's nucleotide sequence