Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7 and GFF8 are separated by 281 nucleotides GFF8 and GFF9 are separated by 88 nucleotides GFF9 and GFF10 are separated by 93 nucleotides GFF10 and GFF11 are separated by 156 nucleotides
GFF7 - hypothetical protein, at 4,389 to 4,778
GFF7
GFF8 - hypothetical protein, at 5,060 to 5,461
GFF8
GFF9 - hypothetical protein, at 5,550 to 5,663
GFF9
GFF10 - hypothetical protein, at 5,757 to 6,065
GFF10
GFF11 - hypothetical protein, at 6,222 to 7,784
GFF11
Position (kb)
5
6
7 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4.757 kb on - strand at 4.793 kb on + strand at 4.801 kb on - strand at 4.858 kb on - strand at 4.858 kb on - strand at 4.872 kb on + strand at 4.888 kb on - strand at 4.968 kb on + strand at 5.014 kb on - strand at 5.080 kb on + strand at 5.124 kb on - strand, within GFF8 at 5.124 kb on - strand, within GFF8 at 5.148 kb on - strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.151 kb on + strand, within GFF8 at 5.154 kb on - strand, within GFF8 at 5.175 kb on + strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.176 kb on + strand, within GFF8 at 5.179 kb on - strand, within GFF8 at 5.192 kb on - strand, within GFF8 at 5.209 kb on + strand, within GFF8 at 5.210 kb on - strand, within GFF8 at 5.212 kb on + strand, within GFF8 at 5.212 kb on + strand, within GFF8 at 5.266 kb on - strand, within GFF8 at 5.279 kb on + strand, within GFF8 at 5.281 kb on - strand, within GFF8 at 5.284 kb on + strand, within GFF8 at 5.295 kb on + strand, within GFF8 at 5.295 kb on + strand, within GFF8 at 5.303 kb on - strand, within GFF8 at 5.308 kb on + strand, within GFF8 at 5.343 kb on + strand, within GFF8 at 5.348 kb on + strand, within GFF8 at 5.348 kb on + strand, within GFF8 at 5.356 kb on - strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.392 kb on + strand, within GFF8 at 5.400 kb on - strand, within GFF8 at 5.407 kb on + strand, within GFF8 at 5.431 kb on + strand at 5.435 kb on + strand at 5.443 kb on - strand at 5.447 kb on - strand at 5.464 kb on - strand at 5.464 kb on - strand at 5.480 kb on + strand at 5.508 kb on - strand at 5.631 kb on + strand, within GFF9 at 5.634 kb on - strand, within GFF9 at 5.634 kb on - strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.636 kb on + strand, within GFF9 at 5.644 kb on - strand, within GFF9 at 5.663 kb on - strand at 5.686 kb on - strand at 5.696 kb on + strand at 5.696 kb on + strand at 5.702 kb on - strand at 5.702 kb on - strand at 5.704 kb on - strand at 5.712 kb on - strand at 5.714 kb on - strand at 5.729 kb on - strand at 5.746 kb on + strand at 5.746 kb on + strand at 5.747 kb on + strand at 5.754 kb on - strand at 5.757 kb on - strand at 5.760 kb on + strand at 5.763 kb on - strand at 5.770 kb on - strand at 5.777 kb on - strand at 5.782 kb on - strand at 5.797 kb on + strand, within GFF10 at 5.817 kb on - strand, within GFF10 at 5.876 kb on + strand, within GFF10 at 5.881 kb on + strand, within GFF10 at 5.891 kb on + strand, within GFF10 at 5.899 kb on - strand, within GFF10 at 5.906 kb on + strand, within GFF10 at 5.914 kb on - strand, within GFF10 at 5.949 kb on + strand, within GFF10 at 5.971 kb on + strand, within GFF10 at 6.041 kb on - strand at 6.047 kb on + strand at 6.183 kb on + strand at 6.245 kb on - strand at 6.273 kb on + strand at 6.273 kb on + strand at 6.273 kb on + strand at 6.281 kb on - strand at 6.281 kb on - strand at 6.281 kb on - strand at 6.281 kb on - strand at 6.286 kb on - strand at 6.297 kb on + strand at 6.316 kb on - strand at 6.322 kb on - strand at 6.341 kb on + strand at 6.349 kb on - strand at 6.349 kb on - strand at 6.351 kb on + strand at 6.354 kb on - strand at 6.356 kb on + strand at 6.356 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.357 kb on + strand at 6.359 kb on - strand at 6.364 kb on - strand at 6.364 kb on - strand at 6.365 kb on - strand at 6.397 kb on + strand, within GFF11 at 6.403 kb on - strand, within GFF11 at 6.405 kb on - strand, within GFF11 at 6.411 kb on - strand, within GFF11 at 6.413 kb on + strand, within GFF11 at 6.497 kb on - strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.519 kb on + strand, within GFF11 at 6.521 kb on + strand, within GFF11 at 6.521 kb on + strand, within GFF11 at 6.521 kb on + strand, within GFF11 at 6.521 kb on + strand, within GFF11 at 6.523 kb on + strand, within GFF11 at 6.527 kb on - strand, within GFF11 at 6.527 kb on - strand, within GFF11 at 6.527 kb on - strand, within GFF11 at 6.529 kb on - strand, within GFF11 at 6.540 kb on - strand, within GFF11 at 6.558 kb on + strand, within GFF11 at 6.558 kb on + strand, within GFF11 at 6.558 kb on + strand, within GFF11 at 6.560 kb on + strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.566 kb on - strand, within GFF11 at 6.582 kb on + strand, within GFF11 at 6.590 kb on - strand, within GFF11 at 6.590 kb on - strand, within GFF11 at 6.618 kb on + strand, within GFF11 at 6.618 kb on + strand, within GFF11 at 6.624 kb on - strand, within GFF11 at 6.624 kb on - strand, within GFF11 at 6.665 kb on - strand, within GFF11 at 6.686 kb on + strand, within GFF11 at 6.705 kb on - strand, within GFF11 at 6.716 kb on - strand, within GFF11 at 6.719 kb on - strand, within GFF11 at 6.727 kb on + strand, within GFF11 at 6.740 kb on - strand, within GFF11 at 6.740 kb on - strand, within GFF11 at 6.764 kb on + strand, within GFF11 at 6.779 kb on + strand, within GFF11 at 6.782 kb on - strand, within GFF11 at 6.787 kb on - strand, within GFF11 at 6.788 kb on + strand, within GFF11 at 6.798 kb on + strand, within GFF11 at 6.865 kb on - strand, within GFF11 at 6.868 kb on + strand, within GFF11 at 6.873 kb on + strand, within GFF11 at 6.873 kb on + strand, within GFF11 at 6.881 kb on - strand, within GFF11 at 6.881 kb on - strand, within GFF11 at 6.885 kb on + strand, within GFF11 at 6.891 kb on + strand, within GFF11 at 6.895 kb on + strand, within GFF11 at 6.905 kb on + strand, within GFF11 at 6.905 kb on + strand, within GFF11 at 6.913 kb on - strand, within GFF11 at 6.914 kb on + strand, within GFF11 at 6.923 kb on + strand, within GFF11 at 6.962 kb on + strand, within GFF11 at 6.965 kb on - strand, within GFF11 at 6.965 kb on - strand, within GFF11 at 6.968 kb on + strand, within GFF11 at 6.968 kb on + strand, within GFF11 at 6.976 kb on - strand, within GFF11 at 6.980 kb on + strand, within GFF11 at 6.985 kb on + strand, within GFF11 at 6.985 kb on + strand, within GFF11 at 6.985 kb on + strand, within GFF11 at 6.985 kb on + strand, within GFF11 at 6.985 kb on + strand, within GFF11 at 6.993 kb on - strand, within GFF11 at 6.995 kb on - strand, within GFF11 at 7.032 kb on + strand, within GFF11 at 7.036 kb on + strand, within GFF11 at 7.037 kb on - strand, within GFF11 at 7.039 kb on - strand, within GFF11 at 7.040 kb on - strand, within GFF11 at 7.043 kb on + strand, within GFF11 at 7.045 kb on - strand, within GFF11 at 7.051 kb on - strand, within GFF11 at 7.063 kb on + strand, within GFF11
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 4,757 - +0.3 4,793 + +0.6 4,801 - -1.2 4,858 - -0.5 4,858 - -0.1 4,872 + +0.5 4,888 - +0.4 4,968 + -0.4 5,014 - -0.2 5,080 + -1.6 5,124 - GFF8 0.16 +0.2 5,124 - GFF8 0.16 +0.2 5,148 - GFF8 0.22 +0.1 5,151 + GFF8 0.23 -0.9 5,151 + GFF8 0.23 +0.5 5,151 + GFF8 0.23 +1.4 5,154 - GFF8 0.23 +0.3 5,175 + GFF8 0.29 -0.1 5,176 + GFF8 0.29 -1.5 5,176 + GFF8 0.29 -1.1 5,179 - GFF8 0.30 -0.3 5,192 - GFF8 0.33 +0.2 5,209 + GFF8 0.37 -1.9 5,210 - GFF8 0.37 -0.7 5,212 + GFF8 0.38 -0.4 5,212 + GFF8 0.38 +0.8 5,266 - GFF8 0.51 -1.2 5,279 + GFF8 0.54 -0.4 5,281 - GFF8 0.55 +1.4 5,284 + GFF8 0.56 -2.1 5,295 + GFF8 0.58 -0.1 5,295 + GFF8 0.58 -1.1 5,303 - GFF8 0.60 -0.6 5,308 + GFF8 0.62 -0.0 5,343 + GFF8 0.70 +0.3 5,348 + GFF8 0.72 +0.1 5,348 + GFF8 0.72 -2.3 5,356 - GFF8 0.74 +1.4 5,392 + GFF8 0.83 -0.1 5,392 + GFF8 0.83 +0.3 5,392 + GFF8 0.83 -0.5 5,400 - GFF8 0.85 -1.0 5,407 + GFF8 0.86 -0.2 5,431 + -1.1 5,435 + -2.2 5,443 - -0.8 5,447 - -1.6 5,464 - -0.6 5,464 - -0.2 5,480 + -1.0 5,508 - +1.1 5,631 + GFF9 0.71 -1.1 5,634 - GFF9 0.74 -2.0 5,634 - GFF9 0.74 +1.5 5,636 + GFF9 0.75 -1.9 5,636 + GFF9 0.75 -0.6 5,636 + GFF9 0.75 +0.3 5,644 - GFF9 0.82 +0.7 5,663 - +0.7 5,686 - +1.1 5,696 + +0.3 5,696 + +0.2 5,702 - -1.1 5,702 - +0.1 5,704 - -1.2 5,712 - +0.7 5,714 - -0.5 5,729 - +0.5 5,746 + -1.7 5,746 + -0.6 5,747 + +0.5 5,754 - +1.7 5,757 - -0.2 5,760 + -0.1 5,763 - +0.9 5,770 - -1.1 5,777 - +0.2 5,782 - +0.1 5,797 + GFF10 0.13 -1.6 5,817 - GFF10 0.19 -1.1 5,876 + GFF10 0.39 +1.0 5,881 + GFF10 0.40 -1.5 5,891 + GFF10 0.43 -0.5 5,899 - GFF10 0.46 +0.3 5,906 + GFF10 0.48 -0.4 5,914 - GFF10 0.51 -0.9 5,949 + GFF10 0.62 -1.5 5,971 + GFF10 0.69 +0.2 6,041 - +0.1 6,047 + +0.1 6,183 + -0.9 6,245 - -1.8 6,273 + +0.4 6,273 + -2.0 6,273 + -1.5 6,281 - -1.6 6,281 - -0.5 6,281 - +0.1 6,281 - -2.5 6,286 - -3.3 6,297 + -1.4 6,316 - +0.3 6,322 - +0.5 6,341 + -0.5 6,349 - -0.9 6,349 - +0.8 6,351 + -2.3 6,354 - -0.3 6,356 + +0.8 6,356 + +0.3 6,357 + -0.6 6,357 + -1.7 6,357 + -1.9 6,357 + -0.2 6,357 + -0.0 6,359 - -1.3 6,364 - -1.9 6,364 - -1.4 6,365 - +0.2 6,397 + GFF11 0.11 -1.1 6,403 - GFF11 0.12 +0.6 6,405 - GFF11 0.12 -1.8 6,411 - GFF11 0.12 -1.3 6,413 + GFF11 0.12 -0.1 6,497 - GFF11 0.18 -1.5 6,519 + GFF11 0.19 +0.6 6,519 + GFF11 0.19 -0.2 6,519 + GFF11 0.19 -0.1 6,519 + GFF11 0.19 -0.1 6,519 + GFF11 0.19 +1.0 6,519 + GFF11 0.19 +1.0 6,521 + GFF11 0.19 -1.6 6,521 + GFF11 0.19 +1.0 6,521 + GFF11 0.19 +0.5 6,521 + GFF11 0.19 -0.4 6,523 + GFF11 0.19 -0.1 6,527 - GFF11 0.20 +0.1 6,527 - GFF11 0.20 -1.7 6,527 - GFF11 0.20 -1.4 6,529 - GFF11 0.20 -0.2 6,540 - GFF11 0.20 -1.1 6,558 + GFF11 0.21 +0.9 6,558 + GFF11 0.21 +1.2 6,558 + GFF11 0.21 +0.4 6,560 + GFF11 0.22 -0.8 6,566 - GFF11 0.22 -1.7 6,566 - GFF11 0.22 +1.2 6,566 - GFF11 0.22 +1.1 6,566 - GFF11 0.22 -1.1 6,566 - GFF11 0.22 -0.0 6,566 - GFF11 0.22 +0.8 6,566 - GFF11 0.22 +1.0 6,566 - GFF11 0.22 -1.3 6,566 - GFF11 0.22 +0.1 6,582 + GFF11 0.23 -0.2 6,590 - GFF11 0.24 -0.7 6,590 - GFF11 0.24 -3.6 6,618 + GFF11 0.25 -0.3 6,618 + GFF11 0.25 -1.2 6,624 - GFF11 0.26 -1.0 6,624 - GFF11 0.26 +0.2 6,665 - GFF11 0.28 +0.5 6,686 + GFF11 0.30 +0.9 6,705 - GFF11 0.31 -0.8 6,716 - GFF11 0.32 +0.6 6,719 - GFF11 0.32 -0.5 6,727 + GFF11 0.32 -0.9 6,740 - GFF11 0.33 +0.8 6,740 - GFF11 0.33 -1.6 6,764 + GFF11 0.35 -1.6 6,779 + GFF11 0.36 -0.6 6,782 - GFF11 0.36 -0.6 6,787 - GFF11 0.36 -0.9 6,788 + GFF11 0.36 -1.7 6,798 + GFF11 0.37 -1.7 6,865 - GFF11 0.41 +1.2 6,868 + GFF11 0.41 -0.9 6,873 + GFF11 0.42 -0.1 6,873 + GFF11 0.42 -1.9 6,881 - GFF11 0.42 +0.8 6,881 - GFF11 0.42 +0.1 6,885 + GFF11 0.42 -0.8 6,891 + GFF11 0.43 -1.5 6,895 + GFF11 0.43 -0.1 6,905 + GFF11 0.44 -1.3 6,905 + GFF11 0.44 -0.4 6,913 - GFF11 0.44 -1.0 6,914 + GFF11 0.44 -0.3 6,923 + GFF11 0.45 -2.4 6,962 + GFF11 0.47 -1.9 6,965 - GFF11 0.48 -0.8 6,965 - GFF11 0.48 +0.4 6,968 + GFF11 0.48 -1.6 6,968 + GFF11 0.48 -1.6 6,976 - GFF11 0.48 +1.1 6,980 + GFF11 0.48 -0.7 6,985 + GFF11 0.49 +0.7 6,985 + GFF11 0.49 +1.2 6,985 + GFF11 0.49 -0.4 6,985 + GFF11 0.49 -1.2 6,985 + GFF11 0.49 +0.5 6,993 - GFF11 0.49 -0.5 6,995 - GFF11 0.49 -0.7 7,032 + GFF11 0.52 -1.1 7,036 + GFF11 0.52 -0.9 7,037 - GFF11 0.52 -0.8 7,039 - GFF11 0.52 -0.3 7,040 - GFF11 0.52 -1.3 7,043 + GFF11 0.53 -0.2 7,045 - GFF11 0.53 +1.1 7,051 - GFF11 0.53 +1.6 7,063 + GFF11 0.54 -0.5
Or see this region's nucleotide sequence