Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF94 and GFF95 are separated by 17 nucleotides GFF95 and GFF96 overlap by 4 nucleotides GFF96 and GFF97 are separated by 52 nucleotides GFF97 and GFF98 overlap by 1 nucleotides
GFF94 - hypothetical protein, at 83,743 to 85,359
GFF94
GFF95 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 85,377 to 86,237
GFF95
GFF96 - Predicted N-formylglutamate amidohydrolase, at 86,234 to 86,950
GFF96
GFF97 - hypothetical protein, at 87,003 to 87,359
GFF97
GFF98 - NAD(P)H-hydrate epimerase (EC 5.1.99.6) / ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136), at 87,359 to 88,750
GFF98
Position (kb)
86
87 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 85.308 kb on + strand at 85.320 kb on + strand at 85.321 kb on - strand at 85.330 kb on + strand at 85.332 kb on + strand at 85.340 kb on - strand at 85.382 kb on - strand at 86.222 kb on + strand at 86.222 kb on + strand at 86.222 kb on + strand at 86.230 kb on - strand at 86.230 kb on - strand at 86.236 kb on + strand at 86.243 kb on + strand at 86.243 kb on + strand at 86.243 kb on + strand at 86.243 kb on + strand at 86.243 kb on + strand at 86.243 kb on + strand at 86.245 kb on + strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.251 kb on - strand at 86.268 kb on + strand at 86.276 kb on - strand at 86.278 kb on + strand at 86.289 kb on - strand at 86.309 kb on + strand, within GFF96 at 86.311 kb on - strand, within GFF96 at 86.319 kb on + strand, within GFF96 at 86.327 kb on - strand, within GFF96 at 86.370 kb on + strand, within GFF96 at 86.386 kb on + strand, within GFF96 at 86.421 kb on + strand, within GFF96 at 86.463 kb on + strand, within GFF96 at 86.473 kb on + strand, within GFF96 at 86.494 kb on + strand, within GFF96 at 86.526 kb on - strand, within GFF96 at 86.549 kb on + strand, within GFF96 at 86.564 kb on + strand, within GFF96 at 86.584 kb on - strand, within GFF96 at 86.585 kb on + strand, within GFF96 at 86.609 kb on + strand, within GFF96 at 86.609 kb on + strand, within GFF96 at 86.609 kb on + strand, within GFF96 at 86.615 kb on + strand, within GFF96 at 86.616 kb on - strand, within GFF96 at 86.623 kb on - strand, within GFF96 at 86.623 kb on - strand, within GFF96 at 86.623 kb on - strand, within GFF96 at 86.638 kb on - strand, within GFF96 at 86.638 kb on - strand, within GFF96 at 86.643 kb on + strand, within GFF96 at 86.686 kb on + strand, within GFF96 at 86.687 kb on - strand, within GFF96 at 86.771 kb on + strand, within GFF96 at 86.776 kb on + strand, within GFF96 at 86.776 kb on + strand, within GFF96 at 86.776 kb on + strand, within GFF96 at 86.776 kb on + strand, within GFF96 at 86.779 kb on - strand, within GFF96 at 86.779 kb on - strand, within GFF96 at 86.781 kb on + strand, within GFF96 at 86.781 kb on + strand, within GFF96 at 86.781 kb on + strand, within GFF96 at 86.781 kb on + strand, within GFF96 at 86.789 kb on - strand, within GFF96 at 86.804 kb on + strand, within GFF96 at 86.804 kb on + strand, within GFF96 at 86.812 kb on - strand, within GFF96 at 86.880 kb on + strand at 86.880 kb on + strand at 86.880 kb on + strand at 86.880 kb on - strand at 86.913 kb on + strand at 86.916 kb on - strand at 86.916 kb on - strand at 86.921 kb on + strand at 86.926 kb on + strand at 86.948 kb on - strand at 86.960 kb on - strand at 86.960 kb on - strand at 86.973 kb on + strand at 86.981 kb on - strand at 86.992 kb on + strand at 86.992 kb on + strand at 86.997 kb on + strand at 87.000 kb on - strand at 87.000 kb on - strand at 87.000 kb on - strand at 87.024 kb on + strand at 87.029 kb on + strand at 87.029 kb on + strand at 87.039 kb on + strand, within GFF97 at 87.039 kb on + strand, within GFF97 at 87.047 kb on - strand, within GFF97 at 87.058 kb on + strand, within GFF97 at 87.058 kb on - strand, within GFF97 at 87.062 kb on + strand, within GFF97 at 87.062 kb on + strand, within GFF97 at 87.070 kb on - strand, within GFF97 at 87.113 kb on - strand, within GFF97 at 87.149 kb on - strand, within GFF97 at 87.171 kb on + strand, within GFF97 at 87.171 kb on + strand, within GFF97 at 87.172 kb on + strand, within GFF97 at 87.173 kb on - strand, within GFF97 at 87.209 kb on + strand, within GFF97 at 87.209 kb on + strand, within GFF97 at 87.209 kb on + strand, within GFF97 at 87.217 kb on - strand, within GFF97 at 87.217 kb on - strand, within GFF97 at 87.219 kb on + strand, within GFF97 at 87.229 kb on + strand, within GFF97 at 87.232 kb on - strand, within GFF97 at 87.232 kb on - strand, within GFF97 at 87.237 kb on - strand, within GFF97 at 87.237 kb on - strand, within GFF97 at 87.239 kb on + strand, within GFF97 at 87.262 kb on + strand, within GFF97 at 87.262 kb on + strand, within GFF97 at 87.268 kb on + strand, within GFF97 at 87.310 kb on + strand, within GFF97 at 87.310 kb on + strand, within GFF97 at 87.313 kb on - strand, within GFF97 at 87.315 kb on + strand, within GFF97 at 87.318 kb on - strand, within GFF97 at 87.327 kb on - strand at 87.354 kb on + strand at 87.354 kb on + strand at 87.394 kb on + strand at 87.435 kb on + strand at 87.435 kb on + strand at 87.443 kb on - strand at 87.443 kb on - strand at 87.443 kb on - strand at 87.443 kb on - strand at 87.443 kb on - strand at 87.445 kb on + strand at 87.470 kb on + strand at 87.486 kb on + strand at 87.507 kb on + strand, within GFF98 at 87.514 kb on - strand, within GFF98 at 87.566 kb on - strand, within GFF98 at 87.573 kb on + strand, within GFF98 at 87.600 kb on + strand, within GFF98 at 87.600 kb on + strand, within GFF98 at 87.610 kb on + strand, within GFF98 at 87.610 kb on + strand, within GFF98 at 87.610 kb on - strand, within GFF98 at 87.612 kb on - strand, within GFF98 at 87.615 kb on + strand, within GFF98 at 87.634 kb on + strand, within GFF98 at 87.634 kb on + strand, within GFF98 at 87.648 kb on - strand, within GFF98 at 87.654 kb on + strand, within GFF98 at 87.662 kb on - strand, within GFF98 at 87.662 kb on - strand, within GFF98 at 87.662 kb on - strand, within GFF98 at 87.705 kb on - strand, within GFF98 at 87.709 kb on + strand, within GFF98 at 87.709 kb on + strand, within GFF98 at 87.709 kb on + strand, within GFF98 at 87.709 kb on + strand, within GFF98 at 87.709 kb on + strand, within GFF98 at 87.715 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.717 kb on - strand, within GFF98 at 87.719 kb on + strand, within GFF98 at 87.719 kb on + strand, within GFF98 at 87.719 kb on + strand, within GFF98 at 87.727 kb on - strand, within GFF98 at 87.727 kb on - strand, within GFF98 at 87.727 kb on - strand, within GFF98 at 87.727 kb on - strand, within GFF98 at 87.727 kb on - strand, within GFF98 at 87.732 kb on + strand, within GFF98 at 87.782 kb on + strand, within GFF98 at 87.803 kb on + strand, within GFF98 at 87.813 kb on + strand, within GFF98 at 87.813 kb on + strand, within GFF98 at 87.813 kb on + strand, within GFF98 at 87.813 kb on + strand, within GFF98 at 87.821 kb on - strand, within GFF98 at 87.821 kb on - strand, within GFF98 at 87.832 kb on - strand, within GFF98 at 87.848 kb on + strand, within GFF98 at 87.856 kb on + strand, within GFF98 at 87.872 kb on + strand, within GFF98 at 87.883 kb on + strand, within GFF98 at 87.883 kb on + strand, within GFF98 at 87.883 kb on + strand, within GFF98 at 87.883 kb on + strand, within GFF98 at 87.883 kb on + strand, within GFF98 at 87.891 kb on - strand, within GFF98 at 87.891 kb on - strand, within GFF98 at 87.891 kb on - strand, within GFF98 at 87.901 kb on + strand, within GFF98 at 87.904 kb on - strand, within GFF98 at 87.926 kb on - strand, within GFF98 at 87.928 kb on - strand, within GFF98 at 87.933 kb on - strand, within GFF98 at 87.939 kb on - strand, within GFF98
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 85,308 + +0.6 85,320 + +0.2 85,321 - -1.4 85,330 + -1.9 85,332 + -0.9 85,340 - +0.1 85,382 - +0.0 86,222 + +0.6 86,222 + -0.2 86,222 + +0.7 86,230 - -0.3 86,230 - +0.3 86,236 + -0.6 86,243 + +0.1 86,243 + +1.0 86,243 + -0.4 86,243 + -1.1 86,243 + +0.4 86,243 + -1.0 86,245 + -1.4 86,251 - -2.1 86,251 - -0.6 86,251 - -2.0 86,251 - +0.7 86,251 - -1.6 86,251 - +1.0 86,251 - -0.1 86,268 + +1.0 86,276 - -1.2 86,278 + +0.4 86,289 - +0.1 86,309 + GFF96 0.10 +0.1 86,311 - GFF96 0.11 -1.2 86,319 + GFF96 0.12 -2.1 86,327 - GFF96 0.13 -0.9 86,370 + GFF96 0.19 +0.6 86,386 + GFF96 0.21 -0.8 86,421 + GFF96 0.26 -1.8 86,463 + GFF96 0.32 +0.5 86,473 + GFF96 0.33 -1.2 86,494 + GFF96 0.36 +1.3 86,526 - GFF96 0.41 -1.4 86,549 + GFF96 0.44 +1.2 86,564 + GFF96 0.46 +0.0 86,584 - GFF96 0.49 -1.1 86,585 + GFF96 0.49 +1.2 86,609 + GFF96 0.52 -1.1 86,609 + GFF96 0.52 -1.5 86,609 + GFF96 0.52 +0.5 86,615 + GFF96 0.53 -1.0 86,616 - GFF96 0.53 +0.0 86,623 - GFF96 0.54 -0.6 86,623 - GFF96 0.54 -1.7 86,623 - GFF96 0.54 -0.3 86,638 - GFF96 0.56 -1.2 86,638 - GFF96 0.56 -0.2 86,643 + GFF96 0.57 +0.9 86,686 + GFF96 0.63 -0.2 86,687 - GFF96 0.63 -2.3 86,771 + GFF96 0.75 +0.2 86,776 + GFF96 0.76 +0.3 86,776 + GFF96 0.76 -1.2 86,776 + GFF96 0.76 -0.6 86,776 + GFF96 0.76 -0.7 86,779 - GFF96 0.76 -0.7 86,779 - GFF96 0.76 -2.1 86,781 + GFF96 0.76 -1.3 86,781 + GFF96 0.76 -1.0 86,781 + GFF96 0.76 -0.3 86,781 + GFF96 0.76 -0.3 86,789 - GFF96 0.77 -0.2 86,804 + GFF96 0.79 +1.1 86,804 + GFF96 0.79 -1.9 86,812 - GFF96 0.81 -0.6 86,880 + -0.2 86,880 + -0.1 86,880 + -1.2 86,880 - -0.2 86,913 + -1.3 86,916 - -0.6 86,916 - +0.4 86,921 + -0.4 86,926 + -2.6 86,948 - -2.7 86,960 - +0.4 86,960 - +1.2 86,973 + +0.5 86,981 - -0.3 86,992 + -0.4 86,992 + +1.1 86,997 + -2.1 87,000 - -0.4 87,000 - -0.7 87,000 - +0.4 87,024 + +0.8 87,029 + -0.3 87,029 + -0.3 87,039 + GFF97 0.10 -0.7 87,039 + GFF97 0.10 +0.4 87,047 - GFF97 0.12 -0.3 87,058 + GFF97 0.15 -0.1 87,058 - GFF97 0.15 -0.9 87,062 + GFF97 0.17 +0.6 87,062 + GFF97 0.17 -2.0 87,070 - GFF97 0.19 -1.7 87,113 - GFF97 0.31 -1.2 87,149 - GFF97 0.41 -0.8 87,171 + GFF97 0.47 -0.8 87,171 + GFF97 0.47 -0.1 87,172 + GFF97 0.47 +0.1 87,173 - GFF97 0.48 -0.4 87,209 + GFF97 0.58 -0.1 87,209 + GFF97 0.58 -0.4 87,209 + GFF97 0.58 -0.2 87,217 - GFF97 0.60 -2.8 87,217 - GFF97 0.60 -1.4 87,219 + GFF97 0.61 +1.0 87,229 + GFF97 0.63 +0.3 87,232 - GFF97 0.64 -1.7 87,232 - GFF97 0.64 -1.4 87,237 - GFF97 0.66 -1.4 87,237 - GFF97 0.66 -0.2 87,239 + GFF97 0.66 -1.6 87,262 + GFF97 0.73 -2.4 87,262 + GFF97 0.73 +0.7 87,268 + GFF97 0.74 +0.2 87,310 + GFF97 0.86 +0.6 87,310 + GFF97 0.86 -1.7 87,313 - GFF97 0.87 -2.0 87,315 + GFF97 0.87 +0.4 87,318 - GFF97 0.88 -0.0 87,327 - -2.3 87,354 + -0.0 87,354 + +0.6 87,394 + -1.0 87,435 + +0.2 87,435 + +1.1 87,443 - +0.2 87,443 - +1.0 87,443 - -0.7 87,443 - -0.7 87,443 - +0.1 87,445 + +1.0 87,470 + -0.5 87,486 + +0.3 87,507 + GFF98 0.11 +0.1 87,514 - GFF98 0.11 -1.6 87,566 - GFF98 0.15 +1.1 87,573 + GFF98 0.15 -0.3 87,600 + GFF98 0.17 +0.4 87,600 + GFF98 0.17 -1.4 87,610 + GFF98 0.18 -0.6 87,610 + GFF98 0.18 +0.1 87,610 - GFF98 0.18 -1.2 87,612 - GFF98 0.18 +0.1 87,615 + GFF98 0.18 -0.3 87,634 + GFF98 0.20 -1.6 87,634 + GFF98 0.20 +1.4 87,648 - GFF98 0.21 -2.1 87,654 + GFF98 0.21 +0.8 87,662 - GFF98 0.22 -0.0 87,662 - GFF98 0.22 -1.5 87,662 - GFF98 0.22 -0.5 87,705 - GFF98 0.25 -1.3 87,709 + GFF98 0.25 -0.5 87,709 + GFF98 0.25 -2.5 87,709 + GFF98 0.25 +0.4 87,709 + GFF98 0.25 -0.8 87,709 + GFF98 0.25 -0.5 87,715 - GFF98 0.26 -2.3 87,717 - GFF98 0.26 +1.5 87,717 - GFF98 0.26 -0.5 87,717 - GFF98 0.26 -2.5 87,717 - GFF98 0.26 +0.0 87,717 - GFF98 0.26 +0.3 87,717 - GFF98 0.26 -0.3 87,719 + GFF98 0.26 -0.6 87,719 + GFF98 0.26 +0.1 87,719 + GFF98 0.26 +1.0 87,727 - GFF98 0.26 +1.4 87,727 - GFF98 0.26 +0.1 87,727 - GFF98 0.26 +1.1 87,727 - GFF98 0.26 -0.6 87,727 - GFF98 0.26 -0.6 87,732 + GFF98 0.27 +0.1 87,782 + GFF98 0.30 +1.0 87,803 + GFF98 0.32 +0.5 87,813 + GFF98 0.33 -2.1 87,813 + GFF98 0.33 +0.1 87,813 + GFF98 0.33 -0.1 87,813 + GFF98 0.33 -0.1 87,821 - GFF98 0.33 -1.2 87,821 - GFF98 0.33 -1.1 87,832 - GFF98 0.34 -0.3 87,848 + GFF98 0.35 -0.6 87,856 + GFF98 0.36 +1.2 87,872 + GFF98 0.37 -0.1 87,883 + GFF98 0.38 +1.3 87,883 + GFF98 0.38 +0.1 87,883 + GFF98 0.38 -2.0 87,883 + GFF98 0.38 +0.3 87,883 + GFF98 0.38 -1.4 87,891 - GFF98 0.38 +0.3 87,891 - GFF98 0.38 +0.1 87,891 - GFF98 0.38 -0.1 87,901 + GFF98 0.39 +0.1 87,904 - GFF98 0.39 -1.6 87,926 - GFF98 0.41 -1.6 87,928 - GFF98 0.41 +0.9 87,933 - GFF98 0.41 +0.4 87,939 - GFF98 0.42 -2.0
Or see this region's nucleotide sequence