Strain Fitness in Sphingobium sp. HT1-2 around GFF94

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF93 and GFF94 are separated by 25 nucleotidesGFF94 and GFF95 are separated by 17 nucleotidesGFF95 and GFF96 overlap by 4 nucleotides GFF93 - Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1), at 82,065 to 83,717 GFF93 GFF94 - hypothetical protein, at 83,743 to 85,359 GFF94 GFF95 - 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (EC 2.7.1.148), at 85,377 to 86,237 GFF95 GFF96 - Predicted N-formylglutamate amidohydrolase, at 86,234 to 86,950 GFF96 Position (kb) 83 84 85 86Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 83.726 kb on + strandat 83.726 kb on + strandat 83.726 kb on + strandat 83.726 kb on + strandat 83.732 kb on - strandat 83.734 kb on - strandat 83.745 kb on + strandat 83.794 kb on + strandat 83.794 kb on + strandat 83.796 kb on + strandat 83.830 kb on + strandat 83.888 kb on - strandat 83.893 kb on - strandat 83.893 kb on - strandat 84.020 kb on + strand, within GFF94at 84.020 kb on + strand, within GFF94at 84.027 kb on - strand, within GFF94at 84.028 kb on - strand, within GFF94at 84.033 kb on + strand, within GFF94at 84.033 kb on + strand, within GFF94at 84.041 kb on - strand, within GFF94at 84.046 kb on + strand, within GFF94at 84.046 kb on + strand, within GFF94at 84.046 kb on + strand, within GFF94at 84.046 kb on + strand, within GFF94at 84.051 kb on + strand, within GFF94at 84.054 kb on - strand, within GFF94at 84.054 kb on - strand, within GFF94at 84.054 kb on - strand, within GFF94at 84.059 kb on - strand, within GFF94at 84.059 kb on - strand, within GFF94at 84.059 kb on - strand, within GFF94at 84.059 kb on - strand, within GFF94at 84.069 kb on + strand, within GFF94at 84.082 kb on + strand, within GFF94at 84.103 kb on + strand, within GFF94at 84.111 kb on + strand, within GFF94at 84.111 kb on + strand, within GFF94at 84.111 kb on + strand, within GFF94at 84.111 kb on + strand, within GFF94at 84.116 kb on + strand, within GFF94at 84.119 kb on - strand, within GFF94at 84.119 kb on - strand, within GFF94at 84.119 kb on - strand, within GFF94at 84.121 kb on + strand, within GFF94at 84.124 kb on - strand, within GFF94at 84.130 kb on - strand, within GFF94at 84.140 kb on + strand, within GFF94at 84.151 kb on + strand, within GFF94at 84.194 kb on + strand, within GFF94at 84.205 kb on + strand, within GFF94at 84.205 kb on + strand, within GFF94at 84.205 kb on + strand, within GFF94at 84.213 kb on - strand, within GFF94at 84.249 kb on - strand, within GFF94at 84.254 kb on - strand, within GFF94at 84.268 kb on + strand, within GFF94at 84.268 kb on + strand, within GFF94at 84.268 kb on + strand, within GFF94at 84.275 kb on - strand, within GFF94at 84.276 kb on - strand, within GFF94at 84.276 kb on - strand, within GFF94at 84.287 kb on - strand, within GFF94at 84.298 kb on + strand, within GFF94at 84.303 kb on + strand, within GFF94at 84.304 kb on + strand, within GFF94at 84.311 kb on - strand, within GFF94at 84.312 kb on - strand, within GFF94at 84.330 kb on - strand, within GFF94at 84.336 kb on - strand, within GFF94at 84.345 kb on + strand, within GFF94at 84.353 kb on + strand, within GFF94at 84.360 kb on - strand, within GFF94at 84.360 kb on - strand, within GFF94at 84.361 kb on - strand, within GFF94at 84.371 kb on + strand, within GFF94at 84.388 kb on + strand, within GFF94at 84.388 kb on + strand, within GFF94at 84.401 kb on - strand, within GFF94at 84.420 kb on + strand, within GFF94at 84.449 kb on + strand, within GFF94at 84.452 kb on - strand, within GFF94at 84.461 kb on + strand, within GFF94at 84.461 kb on + strand, within GFF94at 84.463 kb on - strand, within GFF94at 84.472 kb on + strand, within GFF94at 84.525 kb on + strand, within GFF94at 84.528 kb on - strand, within GFF94at 84.551 kb on - strand, within GFF94at 84.569 kb on - strand, within GFF94at 84.579 kb on - strand, within GFF94at 84.651 kb on + strand, within GFF94at 84.670 kb on + strand, within GFF94at 84.698 kb on + strand, within GFF94at 84.701 kb on - strand, within GFF94at 84.710 kb on - strand, within GFF94at 84.718 kb on + strand, within GFF94at 84.718 kb on + strand, within GFF94at 84.742 kb on + strand, within GFF94at 84.801 kb on - strand, within GFF94at 84.849 kb on - strand, within GFF94at 84.874 kb on - strand, within GFF94at 84.882 kb on + strand, within GFF94at 84.890 kb on - strand, within GFF94at 84.890 kb on - strand, within GFF94at 84.895 kb on - strand, within GFF94at 84.909 kb on - strand, within GFF94at 84.914 kb on + strand, within GFF94at 84.914 kb on + strand, within GFF94at 84.924 kb on + strand, within GFF94at 84.932 kb on - strand, within GFF94at 84.937 kb on + strand, within GFF94at 84.937 kb on + strand, within GFF94at 84.937 kb on + strand, within GFF94at 84.947 kb on + strand, within GFF94at 84.950 kb on - strand, within GFF94at 84.962 kb on - strand, within GFF94at 85.010 kb on + strand, within GFF94at 85.018 kb on - strand, within GFF94at 85.023 kb on - strand, within GFF94at 85.023 kb on - strand, within GFF94at 85.023 kb on - strand, within GFF94at 85.028 kb on - strand, within GFF94at 85.037 kb on + strand, within GFF94at 85.056 kb on - strand, within GFF94at 85.116 kb on - strand, within GFF94at 85.117 kb on - strand, within GFF94at 85.127 kb on - strand, within GFF94at 85.133 kb on - strand, within GFF94at 85.138 kb on + strand, within GFF94at 85.140 kb on + strand, within GFF94at 85.140 kb on + strand, within GFF94at 85.140 kb on + strand, within GFF94at 85.140 kb on + strand, within GFF94at 85.148 kb on + strand, within GFF94at 85.148 kb on - strand, within GFF94at 85.148 kb on - strand, within GFF94at 85.161 kb on - strand, within GFF94at 85.210 kb on + strandat 85.210 kb on + strandat 85.210 kb on + strandat 85.218 kb on - strandat 85.218 kb on - strandat 85.218 kb on - strandat 85.225 kb on + strandat 85.225 kb on + strandat 85.308 kb on + strandat 85.320 kb on + strandat 85.321 kb on - strandat 85.330 kb on + strandat 85.332 kb on + strandat 85.340 kb on - strandat 85.382 kb on - strandat 86.222 kb on + strandat 86.222 kb on + strandat 86.222 kb on + strandat 86.230 kb on - strandat 86.230 kb on - strandat 86.236 kb on + strandat 86.243 kb on + strandat 86.243 kb on + strandat 86.243 kb on + strandat 86.243 kb on + strandat 86.243 kb on + strandat 86.243 kb on + strandat 86.245 kb on + strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.251 kb on - strandat 86.268 kb on + strandat 86.276 kb on - strandat 86.278 kb on + strandat 86.289 kb on - strandat 86.309 kb on + strand, within GFF96at 86.311 kb on - strand, within GFF96at 86.319 kb on + strand, within GFF96at 86.327 kb on - strand, within GFF96

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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83,726 + -2.5
83,726 + -0.9
83,726 + -0.9
83,726 + +0.1
83,732 - +0.8
83,734 - +0.4
83,745 + -0.5
83,794 + -1.3
83,794 + -1.5
83,796 + -1.2
83,830 + -1.0
83,888 - +1.0
83,893 - -0.2
83,893 - -0.2
84,020 + GFF94 0.17 +0.4
84,020 + GFF94 0.17 -1.4
84,027 - GFF94 0.18 -0.3
84,028 - GFF94 0.18 +0.2
84,033 + GFF94 0.18 -0.2
84,033 + GFF94 0.18 -1.8
84,041 - GFF94 0.18 -1.3
84,046 + GFF94 0.19 -1.2
84,046 + GFF94 0.19 -0.9
84,046 + GFF94 0.19 -0.7
84,046 + GFF94 0.19 -1.2
84,051 + GFF94 0.19 +0.9
84,054 - GFF94 0.19 -0.7
84,054 - GFF94 0.19 -0.1
84,054 - GFF94 0.19 -0.5
84,059 - GFF94 0.20 -0.1
84,059 - GFF94 0.20 -0.1
84,059 - GFF94 0.20 -0.4
84,059 - GFF94 0.20 +0.0
84,069 + GFF94 0.20 +0.8
84,082 + GFF94 0.21 -1.5
84,103 + GFF94 0.22 -0.9
84,111 + GFF94 0.23 +0.1
84,111 + GFF94 0.23 +0.2
84,111 + GFF94 0.23 +2.0
84,111 + GFF94 0.23 +1.9
84,116 + GFF94 0.23 -2.8
84,119 - GFF94 0.23 +0.7
84,119 - GFF94 0.23 -0.4
84,119 - GFF94 0.23 +0.8
84,121 + GFF94 0.23 -0.5
84,124 - GFF94 0.24 -0.0
84,130 - GFF94 0.24 -0.1
84,140 + GFF94 0.25 -1.8
84,151 + GFF94 0.25 -0.6
84,194 + GFF94 0.28 -0.5
84,205 + GFF94 0.29 -0.0
84,205 + GFF94 0.29 +0.3
84,205 + GFF94 0.29 -0.2
84,213 - GFF94 0.29 -1.6
84,249 - GFF94 0.31 +0.4
84,254 - GFF94 0.32 -1.2
84,268 + GFF94 0.32 -1.7
84,268 + GFF94 0.32 -0.2
84,268 + GFF94 0.32 +0.9
84,275 - GFF94 0.33 +1.1
84,276 - GFF94 0.33 -0.5
84,276 - GFF94 0.33 -0.4
84,287 - GFF94 0.34 +0.3
84,298 + GFF94 0.34 -1.0
84,303 + GFF94 0.35 +0.7
84,304 + GFF94 0.35 +0.8
84,311 - GFF94 0.35 -1.2
84,312 - GFF94 0.35 -0.2
84,330 - GFF94 0.36 +0.5
84,336 - GFF94 0.37 -1.4
84,345 + GFF94 0.37 +0.9
84,353 + GFF94 0.38 -0.3
84,360 - GFF94 0.38 +0.7
84,360 - GFF94 0.38 +0.5
84,361 - GFF94 0.38 +0.9
84,371 + GFF94 0.39 -2.7
84,388 + GFF94 0.40 -0.3
84,388 + GFF94 0.40 -1.5
84,401 - GFF94 0.41 -0.3
84,420 + GFF94 0.42 -0.3
84,449 + GFF94 0.44 -0.2
84,452 - GFF94 0.44 -1.5
84,461 + GFF94 0.44 +0.3
84,461 + GFF94 0.44 +0.4
84,463 - GFF94 0.45 -0.4
84,472 + GFF94 0.45 +0.5
84,525 + GFF94 0.48 +0.0
84,528 - GFF94 0.49 +0.7
84,551 - GFF94 0.50 -0.2
84,569 - GFF94 0.51 +0.1
84,579 - GFF94 0.52 -1.2
84,651 + GFF94 0.56 -0.1
84,670 + GFF94 0.57 +1.5
84,698 + GFF94 0.59 -0.2
84,701 - GFF94 0.59 +0.7
84,710 - GFF94 0.60 -1.6
84,718 + GFF94 0.60 -0.8
84,718 + GFF94 0.60 -0.3
84,742 + GFF94 0.62 -0.2
84,801 - GFF94 0.65 +0.1
84,849 - GFF94 0.68 -0.1
84,874 - GFF94 0.70 -0.2
84,882 + GFF94 0.70 -0.0
84,890 - GFF94 0.71 +1.4
84,890 - GFF94 0.71 -2.0
84,895 - GFF94 0.71 -0.0
84,909 - GFF94 0.72 -0.5
84,914 + GFF94 0.72 +0.3
84,914 + GFF94 0.72 -0.4
84,924 + GFF94 0.73 -1.6
84,932 - GFF94 0.74 -1.4
84,937 + GFF94 0.74 -0.6
84,937 + GFF94 0.74 -0.0
84,937 + GFF94 0.74 -0.9
84,947 + GFF94 0.74 -1.7
84,950 - GFF94 0.75 -0.0
84,962 - GFF94 0.75 +0.8
85,010 + GFF94 0.78 -0.6
85,018 - GFF94 0.79 +1.9
85,023 - GFF94 0.79 -0.7
85,023 - GFF94 0.79 +0.4
85,023 - GFF94 0.79 -2.1
85,028 - GFF94 0.79 -0.4
85,037 + GFF94 0.80 +1.5
85,056 - GFF94 0.81 -0.8
85,116 - GFF94 0.85 -2.3
85,117 - GFF94 0.85 +0.7
85,127 - GFF94 0.86 +1.0
85,133 - GFF94 0.86 +0.9
85,138 + GFF94 0.86 -0.9
85,140 + GFF94 0.86 +0.9
85,140 + GFF94 0.86 -1.8
85,140 + GFF94 0.86 -0.4
85,140 + GFF94 0.86 -0.5
85,148 + GFF94 0.87 +0.4
85,148 - GFF94 0.87 -0.4
85,148 - GFF94 0.87 -0.1
85,161 - GFF94 0.88 -0.6
85,210 + +1.8
85,210 + -0.9
85,210 + -0.9
85,218 - -1.5
85,218 - -0.7
85,218 - -0.6
85,225 + -0.2
85,225 + +1.0
85,308 + +0.6
85,320 + +0.2
85,321 - -1.4
85,330 + -1.9
85,332 + -0.9
85,340 - +0.1
85,382 - +0.0
86,222 + +0.6
86,222 + -0.2
86,222 + +0.7
86,230 - -0.3
86,230 - +0.3
86,236 + -0.6
86,243 + +0.1
86,243 + +1.0
86,243 + -0.4
86,243 + -1.1
86,243 + +0.4
86,243 + -1.0
86,245 + -1.4
86,251 - -2.1
86,251 - -0.6
86,251 - -2.0
86,251 - +0.7
86,251 - -1.6
86,251 - +1.0
86,251 - -0.1
86,268 + +1.0
86,276 - -1.2
86,278 + +0.4
86,289 - +0.1
86,309 + GFF96 0.10 +0.1
86,311 - GFF96 0.11 -1.2
86,319 + GFF96 0.12 -2.1
86,327 - GFF96 0.13 -0.9

Or see this region's nucleotide sequence