Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3 and GFF4 are separated by 149 nucleotides GFF4 and GFF5 are separated by 2 nucleotides GFF5 and GFF6 are separated by 455 nucleotides GFF6 and GFF7 are separated by 157 nucleotides
GFF3 - hypothetical protein, at 2,156 to 2,869
GFF3
GFF4 - Cytotoxic translational repressor of toxin- antitoxin stability system, at 3,019 to 3,348
GFF4
GFF5 - DNA-binding protein, at 3,351 to 3,650
GFF5
GFF6 - hypothetical protein, at 4,106 to 4,231
GFF6
GFF7 - hypothetical protein, at 4,389 to 4,778
GFF7
Position (kb)
3
4 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.367 kb on + strand, within GFF3 at 2.375 kb on - strand, within GFF3 at 2.377 kb on + strand, within GFF3 at 2.385 kb on - strand, within GFF3 at 2.390 kb on + strand, within GFF3 at 2.397 kb on - strand, within GFF3 at 2.411 kb on - strand, within GFF3 at 2.436 kb on + strand, within GFF3 at 2.466 kb on - strand, within GFF3 at 2.487 kb on - strand, within GFF3 at 2.492 kb on + strand, within GFF3 at 2.494 kb on + strand, within GFF3 at 2.502 kb on - strand, within GFF3 at 2.561 kb on - strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.563 kb on + strand, within GFF3 at 2.566 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.571 kb on - strand, within GFF3 at 2.606 kb on - strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.618 kb on + strand, within GFF3 at 2.626 kb on + strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.626 kb on - strand, within GFF3 at 2.627 kb on + strand, within GFF3 at 2.640 kb on + strand, within GFF3 at 2.654 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.662 kb on + strand, within GFF3 at 2.670 kb on - strand, within GFF3 at 2.702 kb on + strand, within GFF3 at 2.744 kb on + strand, within GFF3 at 2.752 kb on - strand, within GFF3 at 2.758 kb on + strand, within GFF3 at 2.766 kb on - strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.783 kb on + strand, within GFF3 at 2.791 kb on - strand, within GFF3 at 2.791 kb on - strand, within GFF3 at 2.801 kb on + strand at 2.894 kb on + strand at 2.902 kb on + strand at 2.931 kb on - strand at 2.931 kb on - strand at 2.937 kb on + strand at 3.017 kb on + strand at 3.017 kb on + strand at 3.038 kb on + strand at 3.040 kb on - strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.043 kb on + strand at 3.046 kb on - strand at 3.051 kb on - strand at 3.077 kb on + strand, within GFF4 at 3.085 kb on - strand, within GFF4 at 3.090 kb on - strand, within GFF4 at 3.092 kb on + strand, within GFF4 at 3.165 kb on + strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.226 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.308 kb on - strand, within GFF4 at 3.452 kb on + strand, within GFF5 at 3.452 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.508 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.540 kb on + strand, within GFF5 at 3.653 kb on - strand at 3.657 kb on - strand at 3.745 kb on - strand at 3.774 kb on - strand at 3.776 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.829 kb on + strand at 3.838 kb on - strand at 3.893 kb on + strand at 3.903 kb on + strand at 3.955 kb on + strand at 3.970 kb on + strand at 3.992 kb on - strand at 3.993 kb on - strand at 4.004 kb on + strand at 4.086 kb on - strand at 4.109 kb on + strand at 4.117 kb on - strand at 4.137 kb on + strand, within GFF6 at 4.153 kb on - strand, within GFF6 at 4.164 kb on - strand, within GFF6 at 4.178 kb on + strand, within GFF6 at 4.212 kb on - strand, within GFF6 at 4.217 kb on - strand, within GFF6 at 4.247 kb on - strand at 4.292 kb on + strand at 4.302 kb on - strand at 4.314 kb on + strand at 4.314 kb on + strand at 4.316 kb on + strand at 4.322 kb on - strand at 4.349 kb on - strand at 4.381 kb on + strand at 4.414 kb on + strand at 4.422 kb on - strand at 4.423 kb on - strand at 4.442 kb on + strand, within GFF7 at 4.521 kb on + strand, within GFF7 at 4.524 kb on - strand, within GFF7 at 4.554 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.588 kb on + strand, within GFF7 at 4.596 kb on - strand, within GFF7 at 4.605 kb on - strand, within GFF7 at 4.647 kb on + strand, within GFF7
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 2,367 + GFF3 0.30 +0.8 2,367 + GFF3 0.30 -0.4 2,367 + GFF3 0.30 -1.4 2,367 + GFF3 0.30 +0.1 2,375 - GFF3 0.31 -0.0 2,377 + GFF3 0.31 +0.3 2,385 - GFF3 0.32 -0.9 2,390 + GFF3 0.33 +0.3 2,397 - GFF3 0.34 -0.9 2,411 - GFF3 0.36 +0.8 2,436 + GFF3 0.39 +0.2 2,466 - GFF3 0.43 +0.2 2,487 - GFF3 0.46 +0.0 2,492 + GFF3 0.47 -1.7 2,494 + GFF3 0.47 +0.0 2,502 - GFF3 0.48 -0.3 2,561 - GFF3 0.57 +0.6 2,563 + GFF3 0.57 -1.5 2,563 + GFF3 0.57 +0.1 2,563 + GFF3 0.57 +1.4 2,563 + GFF3 0.57 +0.5 2,563 + GFF3 0.57 -0.1 2,566 - GFF3 0.57 +0.3 2,571 - GFF3 0.58 +0.2 2,571 - GFF3 0.58 -0.4 2,571 - GFF3 0.58 -0.2 2,571 - GFF3 0.58 -0.8 2,606 - GFF3 0.63 +0.6 2,618 + GFF3 0.65 +1.0 2,618 + GFF3 0.65 -1.5 2,618 + GFF3 0.65 +0.5 2,618 + GFF3 0.65 +0.9 2,626 + GFF3 0.66 -2.2 2,626 - GFF3 0.66 +0.4 2,626 - GFF3 0.66 +0.2 2,626 - GFF3 0.66 -0.0 2,626 - GFF3 0.66 -0.7 2,627 + GFF3 0.66 -0.5 2,640 + GFF3 0.68 -2.2 2,654 + GFF3 0.70 -1.3 2,662 + GFF3 0.71 +0.5 2,662 + GFF3 0.71 +0.0 2,662 + GFF3 0.71 -0.6 2,670 - GFF3 0.72 +0.0 2,702 + GFF3 0.76 +0.3 2,744 + GFF3 0.82 +0.1 2,752 - GFF3 0.83 -0.0 2,758 + GFF3 0.84 +1.0 2,766 - GFF3 0.85 +0.8 2,783 + GFF3 0.88 -0.4 2,783 + GFF3 0.88 +1.2 2,783 + GFF3 0.88 -0.4 2,783 + GFF3 0.88 +0.0 2,783 + GFF3 0.88 -1.2 2,783 + GFF3 0.88 -0.7 2,791 - GFF3 0.89 +2.7 2,791 - GFF3 0.89 -0.1 2,801 + -1.2 2,894 + +0.7 2,902 + -1.3 2,931 - -0.4 2,931 - +0.7 2,937 + -1.4 3,017 + +0.5 3,017 + +1.9 3,038 + -1.2 3,040 - -0.3 3,043 + -0.3 3,043 + -1.4 3,043 + -0.7 3,043 + -1.6 3,043 + +0.4 3,043 + -0.3 3,043 + +1.1 3,046 - -0.5 3,051 - +0.1 3,077 + GFF4 0.18 +0.5 3,085 - GFF4 0.20 +0.6 3,090 - GFF4 0.22 -0.0 3,092 + GFF4 0.22 +0.9 3,165 + GFF4 0.44 -0.6 3,226 - GFF4 0.63 -1.1 3,226 - GFF4 0.63 -0.2 3,308 - GFF4 0.88 -0.6 3,308 - GFF4 0.88 -1.3 3,452 + GFF5 0.34 +0.1 3,452 + GFF5 0.34 +0.4 3,508 + GFF5 0.52 -2.2 3,508 + GFF5 0.52 -1.5 3,540 + GFF5 0.63 -1.2 3,540 + GFF5 0.63 -1.7 3,653 - +0.6 3,657 - -0.7 3,745 - -0.0 3,774 - +0.4 3,776 + -1.0 3,824 + +0.5 3,824 + -0.4 3,824 + -0.2 3,829 + -0.0 3,829 + +1.1 3,829 + -0.2 3,838 - -0.1 3,893 + +0.7 3,903 + +0.1 3,955 + -0.8 3,970 + -0.8 3,992 - +0.4 3,993 - -0.9 4,004 + +0.1 4,086 - -0.5 4,109 + -1.9 4,117 - +0.8 4,137 + GFF6 0.25 -1.6 4,153 - GFF6 0.37 +0.5 4,164 - GFF6 0.46 +0.6 4,178 + GFF6 0.57 -0.8 4,212 - GFF6 0.84 -2.0 4,217 - GFF6 0.88 +0.8 4,247 - -1.5 4,292 + -0.8 4,302 - -1.0 4,314 + -1.3 4,314 + -0.4 4,316 + -0.6 4,322 - -1.2 4,349 - -0.2 4,381 + +0.3 4,414 + -1.3 4,422 - -1.4 4,423 - +1.2 4,442 + GFF7 0.14 -0.3 4,521 + GFF7 0.34 -0.0 4,524 - GFF7 0.35 +1.7 4,554 + GFF7 0.42 -1.7 4,588 + GFF7 0.51 -0.2 4,588 + GFF7 0.51 +0.6 4,588 + GFF7 0.51 -0.2 4,588 + GFF7 0.51 +0.0 4,588 + GFF7 0.51 +0.7 4,588 + GFF7 0.51 -0.2 4,588 + GFF7 0.51 -0.7 4,588 + GFF7 0.51 -0.1 4,596 - GFF7 0.53 -0.7 4,605 - GFF7 0.55 +1.1 4,647 + GFF7 0.66 -0.2
Or see this region's nucleotide sequence