Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF46 and GFF47 are separated by 74 nucleotides GFF47 and GFF48 are separated by 4 nucleotides GFF48 and GFF49 are separated by 69 nucleotides GFF49 and GFF50 are separated by 66 nucleotides
GFF46 - Alkaline phosphodiesterase I (EC 3.1.4.1) / Nucleotide pyrophosphatase (EC 3.6.1.9), at 36,290 to 37,570
GFF46
GFF47 - G:T/U mismatch-specific uracil/thymine DNA-glycosylase (EC 3.2.2.27), at 37,645 to 38,172
GFF47
GFF48 - GTP-binding and nucleic acid-binding protein YchF, at 38,177 to 39,277
GFF48
GFF49 - hypothetical protein, at 39,347 to 40,057
GFF49
GFF50 - Phosphoglucomutase (EC 5.4.2.2), at 40,124 to 41,752
GFF50
Position (kb)
38
39
40 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 37.177 kb on + strand, within GFF46 at 37.182 kb on + strand, within GFF46 at 37.186 kb on + strand, within GFF46 at 37.194 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46 at 37.284 kb on - strand, within GFF46 at 37.320 kb on + strand, within GFF46 at 37.334 kb on + strand, within GFF46 at 37.338 kb on - strand, within GFF46 at 37.351 kb on - strand, within GFF46 at 37.356 kb on - strand, within GFF46 at 37.375 kb on + strand, within GFF46 at 37.381 kb on + strand, within GFF46 at 37.382 kb on - strand, within GFF46 at 37.389 kb on - strand, within GFF46 at 37.535 kb on - strand at 37.537 kb on + strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.545 kb on - strand at 37.566 kb on - strand at 37.568 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.604 kb on + strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.612 kb on - strand at 37.614 kb on - strand at 37.622 kb on - strand at 37.622 kb on - strand at 37.635 kb on + strand at 37.651 kb on - strand at 37.655 kb on - strand at 37.661 kb on - strand at 37.665 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.676 kb on + strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.684 kb on - strand at 37.727 kb on + strand, within GFF47 at 37.735 kb on - strand, within GFF47 at 37.735 kb on - strand, within GFF47 at 37.737 kb on + strand, within GFF47 at 37.750 kb on - strand, within GFF47 at 37.780 kb on + strand, within GFF47 at 37.837 kb on + strand, within GFF47 at 37.845 kb on - strand, within GFF47 at 37.858 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.863 kb on + strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.871 kb on - strand, within GFF47 at 37.885 kb on + strand, within GFF47 at 37.888 kb on - strand, within GFF47 at 37.888 kb on - strand, within GFF47 at 37.890 kb on - strand, within GFF47 at 37.920 kb on + strand, within GFF47 at 37.924 kb on + strand, within GFF47 at 37.924 kb on + strand, within GFF47 at 37.929 kb on + strand, within GFF47 at 37.929 kb on + strand, within GFF47 at 37.939 kb on - strand, within GFF47 at 37.943 kb on + strand, within GFF47 at 37.947 kb on - strand, within GFF47 at 37.952 kb on + strand, within GFF47 at 37.971 kb on + strand, within GFF47 at 37.974 kb on - strand, within GFF47 at 37.981 kb on + strand, within GFF47 at 37.981 kb on + strand, within GFF47 at 37.984 kb on - strand, within GFF47 at 37.989 kb on - strand, within GFF47 at 38.011 kb on - strand, within GFF47 at 38.021 kb on - strand, within GFF47 at 38.024 kb on + strand, within GFF47 at 38.045 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.063 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.117 kb on - strand, within GFF47 at 38.142 kb on + strand at 38.142 kb on + strand at 38.175 kb on + strand at 38.295 kb on + strand, within GFF48 at 38.478 kb on + strand, within GFF48 at 38.632 kb on - strand, within GFF48 at 38.922 kb on - strand, within GFF48 at 38.927 kb on - strand, within GFF48 at 39.014 kb on + strand, within GFF48 at 39.027 kb on - strand, within GFF48 at 39.029 kb on - strand, within GFF48 at 39.068 kb on + strand, within GFF48 at 39.216 kb on + strand at 39.226 kb on + strand at 39.334 kb on + strand at 39.362 kb on - strand at 39.399 kb on + strand at 39.407 kb on - strand at 39.444 kb on - strand, within GFF49 at 39.536 kb on + strand, within GFF49 at 39.549 kb on + strand, within GFF49 at 39.582 kb on + strand, within GFF49 at 39.590 kb on - strand, within GFF49 at 39.608 kb on - strand, within GFF49 at 39.629 kb on - strand, within GFF49 at 39.653 kb on + strand, within GFF49 at 39.661 kb on - strand, within GFF49 at 39.691 kb on + strand, within GFF49 at 39.699 kb on - strand, within GFF49 at 39.721 kb on + strand, within GFF49 at 39.721 kb on + strand, within GFF49 at 39.729 kb on - strand, within GFF49 at 39.731 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.780 kb on + strand, within GFF49 at 39.787 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.788 kb on - strand, within GFF49 at 39.794 kb on - strand, within GFF49 at 39.804 kb on - strand, within GFF49 at 39.831 kb on + strand, within GFF49 at 39.836 kb on + strand, within GFF49 at 39.836 kb on + strand, within GFF49 at 39.839 kb on - strand, within GFF49 at 39.842 kb on + strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.844 kb on - strand, within GFF49 at 39.845 kb on - strand, within GFF49 at 39.856 kb on - strand, within GFF49 at 39.894 kb on + strand, within GFF49 at 39.914 kb on + strand, within GFF49 at 39.939 kb on + strand, within GFF49 at 39.947 kb on - strand, within GFF49 at 39.978 kb on + strand, within GFF49 at 39.998 kb on + strand at 39.998 kb on + strand at 40.006 kb on - strand at 40.006 kb on - strand at 40.006 kb on - strand at 40.065 kb on + strand at 40.073 kb on - strand at 40.091 kb on - strand at 40.102 kb on + strand at 40.130 kb on - strand at 40.135 kb on - strand at 40.135 kb on - strand at 40.139 kb on + strand at 40.142 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.147 kb on - strand at 40.152 kb on - strand at 40.268 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 37,177 + GFF46 0.69 +0.1 37,182 + GFF46 0.70 +0.8 37,186 + GFF46 0.70 +0.4 37,194 - GFF46 0.71 -0.2 37,204 - GFF46 0.71 -1.0 37,204 - GFF46 0.71 +1.4 37,284 - GFF46 0.78 -0.7 37,320 + GFF46 0.80 -1.3 37,334 + GFF46 0.81 -0.8 37,338 - GFF46 0.82 -0.4 37,351 - GFF46 0.83 +0.5 37,356 - GFF46 0.83 +0.8 37,375 + GFF46 0.85 -0.1 37,381 + GFF46 0.85 -0.6 37,382 - GFF46 0.85 -0.6 37,389 - GFF46 0.86 +0.9 37,535 - +0.9 37,537 + +0.5 37,545 - +1.3 37,545 - -1.2 37,545 - -0.6 37,545 - +0.4 37,545 - -1.3 37,545 - -0.3 37,566 - -1.5 37,568 + -0.4 37,604 + +0.7 37,604 + -0.1 37,604 + +0.4 37,604 + +0.5 37,604 + +0.5 37,612 - +0.9 37,612 - +1.2 37,612 - -0.3 37,612 - +0.1 37,614 - +0.2 37,622 - +0.1 37,622 - +0.2 37,635 + -1.0 37,651 - -0.4 37,655 - +1.2 37,661 - +0.4 37,665 + +1.5 37,676 + +0.2 37,676 + -0.7 37,676 + -1.0 37,676 + -0.9 37,676 + +0.1 37,684 - -0.2 37,684 - -1.1 37,684 - +0.7 37,684 - -0.2 37,684 - +1.1 37,727 + GFF47 0.16 -0.4 37,735 - GFF47 0.17 +1.4 37,735 - GFF47 0.17 -1.2 37,737 + GFF47 0.17 +1.1 37,750 - GFF47 0.20 +0.5 37,780 + GFF47 0.26 -1.8 37,837 + GFF47 0.36 -0.8 37,845 - GFF47 0.38 +0.0 37,858 + GFF47 0.40 -1.1 37,863 + GFF47 0.41 -0.4 37,863 + GFF47 0.41 -1.3 37,863 + GFF47 0.41 +1.0 37,863 + GFF47 0.41 +0.5 37,871 - GFF47 0.43 -1.6 37,871 - GFF47 0.43 +0.2 37,871 - GFF47 0.43 +0.5 37,871 - GFF47 0.43 +0.2 37,885 + GFF47 0.45 +0.7 37,888 - GFF47 0.46 -0.4 37,888 - GFF47 0.46 +0.2 37,890 - GFF47 0.46 +0.1 37,920 + GFF47 0.52 +0.5 37,924 + GFF47 0.53 -1.4 37,924 + GFF47 0.53 +0.4 37,929 + GFF47 0.54 +0.2 37,929 + GFF47 0.54 +1.0 37,939 - GFF47 0.56 -0.4 37,943 + GFF47 0.56 -0.6 37,947 - GFF47 0.57 -0.1 37,952 + GFF47 0.58 -0.3 37,971 + GFF47 0.62 +1.2 37,974 - GFF47 0.62 -0.1 37,981 + GFF47 0.64 -0.5 37,981 + GFF47 0.64 -1.5 37,984 - GFF47 0.64 -1.3 37,989 - GFF47 0.65 +0.3 38,011 - GFF47 0.69 -0.0 38,021 - GFF47 0.71 -0.4 38,024 + GFF47 0.72 -2.2 38,045 - GFF47 0.76 -0.1 38,063 - GFF47 0.79 +1.1 38,063 - GFF47 0.79 +2.1 38,063 - GFF47 0.79 +0.9 38,117 - GFF47 0.89 +1.1 38,117 - GFF47 0.89 +1.7 38,117 - GFF47 0.89 +1.3 38,142 + +0.4 38,142 + -0.5 38,175 + +0.5 38,295 + GFF48 0.11 +0.1 38,478 + GFF48 0.27 -0.2 38,632 - GFF48 0.41 +1.4 38,922 - GFF48 0.68 +0.1 38,927 - GFF48 0.68 -0.6 39,014 + GFF48 0.76 +2.0 39,027 - GFF48 0.77 +0.4 39,029 - GFF48 0.77 -0.6 39,068 + GFF48 0.81 +0.4 39,216 + +0.5 39,226 + +0.4 39,334 + +0.3 39,362 - +0.4 39,399 + +1.0 39,407 - +1.1 39,444 - GFF49 0.14 +0.4 39,536 + GFF49 0.27 +1.8 39,549 + GFF49 0.28 -0.0 39,582 + GFF49 0.33 -0.5 39,590 - GFF49 0.34 +1.2 39,608 - GFF49 0.37 +1.1 39,629 - GFF49 0.40 +1.0 39,653 + GFF49 0.43 +0.2 39,661 - GFF49 0.44 +0.4 39,691 + GFF49 0.48 -0.1 39,699 - GFF49 0.50 -0.8 39,721 + GFF49 0.53 -1.1 39,721 + GFF49 0.53 -0.4 39,729 - GFF49 0.54 -0.6 39,731 + GFF49 0.54 +0.1 39,780 + GFF49 0.61 -1.0 39,780 + GFF49 0.61 +0.2 39,780 + GFF49 0.61 -2.4 39,780 + GFF49 0.61 +2.2 39,787 - GFF49 0.62 +0.0 39,788 - GFF49 0.62 +2.2 39,788 - GFF49 0.62 +0.2 39,788 - GFF49 0.62 +0.1 39,794 - GFF49 0.63 -0.8 39,804 - GFF49 0.64 -1.4 39,831 + GFF49 0.68 +0.9 39,836 + GFF49 0.69 +0.4 39,836 + GFF49 0.69 +1.5 39,839 - GFF49 0.69 +1.4 39,842 + GFF49 0.70 -0.6 39,844 - GFF49 0.70 -1.3 39,844 - GFF49 0.70 -1.4 39,844 - GFF49 0.70 -0.0 39,844 - GFF49 0.70 -0.4 39,845 - GFF49 0.70 +0.9 39,856 - GFF49 0.72 -1.8 39,894 + GFF49 0.77 -1.9 39,914 + GFF49 0.80 +1.1 39,939 + GFF49 0.83 -1.2 39,947 - GFF49 0.84 +1.3 39,978 + GFF49 0.89 +0.7 39,998 + -0.7 39,998 + -0.5 40,006 - -0.4 40,006 - +0.2 40,006 - -0.6 40,065 + +0.5 40,073 - -0.1 40,091 - -0.3 40,102 + +0.7 40,130 - +0.4 40,135 - +0.3 40,135 - -1.2 40,139 + +0.1 40,142 - +0.2 40,147 - +0.6 40,147 - -0.1 40,147 - +0.0 40,147 - +0.0 40,147 - +0.0 40,147 - -0.6 40,152 - -0.7 40,268 - +0.4
Or see this region's nucleotide sequence