Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF249 and GFF250 are separated by 89 nucleotides GFF250 and GFF251 overlap by 1 nucleotides GFF251 and GFF252 are separated by 74 nucleotides GFF252 and GFF253 are separated by 6 nucleotides GFF253 and GFF254 are separated by 98 nucleotides
GFF249 - Excinuclease ABC, C subunit-like, at 254,392 to 254,682
GFF249
GFF250 - hypothetical protein, at 254,772 to 255,302
GFF250
GFF251 - Site-specific tyrosine recombinase XerC, at 255,302 to 256,204
GFF251
GFF252 - hypothetical protein, at 256,279 to 256,572
GFF252
GFF253 - DedA, at 256,579 to 256,719
GFF253
GFF254 - hypothetical protein, at 256,818 to 258,431
GFF254
Position (kb) 255
256
257 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 254.385 kb on + strand at 254.405 kb on + strand at 254.412 kb on - strand at 254.424 kb on + strand, within GFF249 at 254.446 kb on - strand, within GFF249 at 254.459 kb on + strand, within GFF249 at 254.477 kb on + strand, within GFF249 at 254.536 kb on + strand, within GFF249 at 254.537 kb on + strand, within GFF249 at 254.544 kb on - strand, within GFF249 at 254.547 kb on + strand, within GFF249 at 254.582 kb on - strand, within GFF249 at 254.593 kb on + strand, within GFF249 at 254.594 kb on - strand, within GFF249 at 254.594 kb on - strand, within GFF249 at 254.594 kb on - strand, within GFF249 at 254.596 kb on + strand, within GFF249 at 254.610 kb on - strand, within GFF249 at 254.640 kb on + strand, within GFF249 at 254.640 kb on + strand, within GFF249 at 254.643 kb on - strand, within GFF249 at 254.648 kb on - strand, within GFF249 at 254.648 kb on - strand, within GFF249 at 254.648 kb on - strand, within GFF249 at 254.648 kb on - strand, within GFF249 at 254.654 kb on + strand at 254.654 kb on + strand at 254.654 kb on + strand at 254.654 kb on + strand at 254.654 kb on + strand at 254.654 kb on + strand at 254.657 kb on - strand at 254.662 kb on - strand at 254.662 kb on - strand at 254.662 kb on - strand at 254.691 kb on + strand at 254.700 kb on - strand at 254.702 kb on + strand at 254.702 kb on + strand at 254.715 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.738 kb on + strand at 254.746 kb on - strand at 254.746 kb on - strand at 254.746 kb on - strand at 254.746 kb on - strand at 254.756 kb on + strand at 254.756 kb on + strand at 254.756 kb on + strand at 254.756 kb on + strand at 254.756 kb on - strand at 254.764 kb on - strand at 254.764 kb on - strand at 254.764 kb on - strand at 254.764 kb on - strand at 254.769 kb on + strand at 254.774 kb on + strand at 254.774 kb on + strand at 254.777 kb on - strand at 254.777 kb on - strand at 254.779 kb on + strand at 254.779 kb on + strand at 254.782 kb on - strand at 254.787 kb on - strand at 254.787 kb on - strand at 254.826 kb on + strand, within GFF250 at 254.839 kb on - strand, within GFF250 at 254.841 kb on + strand, within GFF250 at 254.841 kb on + strand, within GFF250 at 254.898 kb on + strand, within GFF250 at 254.898 kb on + strand, within GFF250 at 254.898 kb on + strand, within GFF250 at 254.903 kb on + strand, within GFF250 at 254.903 kb on + strand, within GFF250 at 254.904 kb on + strand, within GFF250 at 254.906 kb on - strand, within GFF250 at 254.907 kb on + strand, within GFF250 at 254.923 kb on + strand, within GFF250 at 254.928 kb on + strand, within GFF250 at 254.931 kb on - strand, within GFF250 at 254.938 kb on + strand, within GFF250 at 254.946 kb on - strand, within GFF250 at 254.954 kb on + strand, within GFF250 at 254.954 kb on + strand, within GFF250 at 254.956 kb on + strand, within GFF250 at 254.962 kb on - strand, within GFF250 at 254.962 kb on - strand, within GFF250 at 254.964 kb on - strand, within GFF250 at 254.972 kb on - strand, within GFF250 at 254.972 kb on - strand, within GFF250 at 254.972 kb on - strand, within GFF250 at 254.972 kb on - strand, within GFF250 at 254.972 kb on - strand, within GFF250 at 254.987 kb on - strand, within GFF250 at 254.989 kb on + strand, within GFF250 at 254.994 kb on + strand, within GFF250 at 255.037 kb on + strand, within GFF250 at 255.037 kb on + strand, within GFF250 at 255.038 kb on + strand, within GFF250 at 255.056 kb on + strand, within GFF250 at 255.086 kb on - strand, within GFF250 at 255.094 kb on + strand, within GFF250 at 255.094 kb on + strand, within GFF250 at 255.102 kb on - strand, within GFF250 at 255.103 kb on + strand, within GFF250 at 255.105 kb on + strand, within GFF250 at 255.122 kb on + strand, within GFF250 at 255.123 kb on - strand, within GFF250 at 255.123 kb on - strand, within GFF250 at 255.138 kb on + strand, within GFF250 at 255.140 kb on + strand, within GFF250 at 255.146 kb on - strand, within GFF250 at 255.153 kb on - strand, within GFF250 at 255.159 kb on - strand, within GFF250 at 255.159 kb on - strand, within GFF250 at 255.209 kb on + strand, within GFF250 at 255.244 kb on + strand, within GFF250 at 255.252 kb on - strand at 255.254 kb on + strand at 255.254 kb on + strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.262 kb on - strand at 255.267 kb on - strand at 255.270 kb on + strand at 255.278 kb on - strand at 255.283 kb on - strand at 255.296 kb on + strand at 255.317 kb on + strand at 255.375 kb on + strand at 255.397 kb on + strand, within GFF251 at 255.441 kb on - strand, within GFF251 at 255.445 kb on + strand, within GFF251 at 255.470 kb on - strand, within GFF251 at 255.560 kb on + strand, within GFF251 at 255.597 kb on + strand, within GFF251 at 255.618 kb on - strand, within GFF251 at 255.635 kb on + strand, within GFF251 at 255.648 kb on - strand, within GFF251 at 255.673 kb on - strand, within GFF251 at 255.707 kb on + strand, within GFF251 at 255.745 kb on + strand, within GFF251 at 255.770 kb on + strand, within GFF251 at 255.810 kb on + strand, within GFF251 at 255.823 kb on - strand, within GFF251 at 255.833 kb on - strand, within GFF251 at 255.852 kb on - strand, within GFF251 at 255.862 kb on - strand, within GFF251 at 255.892 kb on - strand, within GFF251 at 255.902 kb on - strand, within GFF251 at 255.961 kb on + strand, within GFF251 at 255.984 kb on + strand, within GFF251 at 255.984 kb on + strand, within GFF251 at 255.984 kb on + strand, within GFF251 at 256.030 kb on - strand, within GFF251 at 256.035 kb on - strand, within GFF251 at 256.040 kb on - strand, within GFF251 at 256.053 kb on - strand, within GFF251 at 256.064 kb on + strand, within GFF251 at 256.093 kb on - strand, within GFF251 at 256.097 kb on + strand, within GFF251 at 256.103 kb on + strand, within GFF251 at 256.106 kb on + strand, within GFF251 at 256.114 kb on - strand at 256.124 kb on - strand at 256.128 kb on + strand at 256.133 kb on + strand at 256.138 kb on + strand at 256.187 kb on + strand at 256.195 kb on - strand at 256.206 kb on - strand at 256.217 kb on - strand at 256.229 kb on - strand at 256.239 kb on - strand at 256.239 kb on - strand at 256.241 kb on + strand at 256.241 kb on + strand at 256.249 kb on - strand at 256.249 kb on - strand at 256.256 kb on + strand at 256.272 kb on + strand at 256.277 kb on + strand at 256.280 kb on - strand at 256.290 kb on + strand at 256.297 kb on + strand at 256.298 kb on - strand at 256.298 kb on - strand at 256.315 kb on - strand, within GFF252 at 256.358 kb on - strand, within GFF252 at 256.359 kb on - strand, within GFF252 at 256.423 kb on - strand, within GFF252 at 256.449 kb on + strand, within GFF252 at 256.463 kb on + strand, within GFF252 at 256.473 kb on + strand, within GFF252 at 256.473 kb on + strand, within GFF252 at 256.482 kb on + strand, within GFF252 at 256.482 kb on + strand, within GFF252 at 256.482 kb on + strand, within GFF252 at 256.482 kb on + strand, within GFF252 at 256.482 kb on + strand, within GFF252 at 256.483 kb on + strand, within GFF252 at 256.483 kb on + strand, within GFF252 at 256.490 kb on - strand, within GFF252 at 256.490 kb on - strand, within GFF252 at 256.490 kb on - strand, within GFF252 at 256.490 kb on - strand, within GFF252 at 256.491 kb on - strand, within GFF252 at 256.507 kb on + strand, within GFF252 at 256.509 kb on - strand, within GFF252 at 256.519 kb on - strand, within GFF252 at 256.532 kb on + strand, within GFF252 at 256.532 kb on - strand, within GFF252 at 256.539 kb on - strand, within GFF252 at 256.539 kb on - strand, within GFF252 at 256.539 kb on - strand, within GFF252 at 256.540 kb on - strand, within GFF252 at 256.540 kb on - strand, within GFF252 at 256.541 kb on + strand, within GFF252 at 256.549 kb on - strand at 256.549 kb on - strand at 256.576 kb on + strand at 256.576 kb on + strand at 256.584 kb on - strand at 256.584 kb on - strand at 256.584 kb on - strand at 256.584 kb on - strand at 256.584 kb on - strand at 256.584 kb on - strand at 256.608 kb on + strand, within GFF253 at 256.616 kb on - strand, within GFF253 at 256.616 kb on - strand, within GFF253 at 256.618 kb on + strand, within GFF253 at 256.618 kb on + strand, within GFF253 at 256.618 kb on + strand, within GFF253 at 256.618 kb on + strand, within GFF253 at 256.618 kb on + strand, within GFF253 at 256.621 kb on - strand, within GFF253 at 256.621 kb on - strand, within GFF253 at 256.621 kb on - strand, within GFF253 at 256.621 kb on - strand, within GFF253 at 256.623 kb on + strand, within GFF253 at 256.623 kb on + strand, within GFF253 at 256.626 kb on - strand, within GFF253 at 256.626 kb on - strand, within GFF253 at 256.626 kb on - strand, within GFF253 at 256.626 kb on - strand, within GFF253 at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.706 kb on + strand at 256.711 kb on + strand at 256.714 kb on - strand at 256.714 kb on - strand at 256.714 kb on - strand at 256.716 kb on + strand at 256.716 kb on + strand at 256.719 kb on - strand at 256.748 kb on + strand at 256.748 kb on + strand at 256.804 kb on + strand at 256.874 kb on + strand at 256.874 kb on + strand at 256.882 kb on - strand at 256.892 kb on + strand at 256.893 kb on + strand at 256.900 kb on - strand at 256.900 kb on - strand at 256.901 kb on + strand at 256.901 kb on + strand at 256.907 kb on - strand at 256.909 kb on - strand at 256.929 kb on + strand at 256.929 kb on + strand at 256.929 kb on + strand at 256.934 kb on + strand at 256.942 kb on - strand at 256.946 kb on + strand at 256.946 kb on + strand at 256.953 kb on + strand at 256.954 kb on - strand at 257.031 kb on - strand, within GFF254 at 257.041 kb on - strand, within GFF254 at 257.046 kb on + strand, within GFF254 at 257.054 kb on - strand, within GFF254 at 257.054 kb on - strand, within GFF254 at 257.091 kb on + strand, within GFF254 at 257.114 kb on - strand, within GFF254 at 257.139 kb on - strand, within GFF254 at 257.139 kb on - strand, within GFF254 at 257.139 kb on - strand, within GFF254 at 257.175 kb on + strand, within GFF254 at 257.181 kb on + strand, within GFF254 at 257.193 kb on - strand, within GFF254
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 254,385 + +0.9 254,405 + -0.5 254,412 - -0.4 254,424 + GFF249 0.11 +0.9 254,446 - GFF249 0.19 +0.3 254,459 + GFF249 0.23 -0.1 254,477 + GFF249 0.29 -0.6 254,536 + GFF249 0.49 +0.1 254,537 + GFF249 0.50 +0.0 254,544 - GFF249 0.52 -0.5 254,547 + GFF249 0.53 +0.2 254,582 - GFF249 0.65 -1.8 254,593 + GFF249 0.69 -1.1 254,594 - GFF249 0.69 +0.6 254,594 - GFF249 0.69 +1.4 254,594 - GFF249 0.69 +0.6 254,596 + GFF249 0.70 -1.6 254,610 - GFF249 0.75 -0.5 254,640 + GFF249 0.85 +2.2 254,640 + GFF249 0.85 -0.7 254,643 - GFF249 0.86 +1.6 254,648 - GFF249 0.88 -0.8 254,648 - GFF249 0.88 +0.7 254,648 - GFF249 0.88 -1.6 254,648 - GFF249 0.88 -0.1 254,654 + -1.0 254,654 + -0.8 254,654 + +0.5 254,654 + +0.7 254,654 + +0.1 254,654 + -0.5 254,657 - -0.5 254,662 - -1.1 254,662 - -1.0 254,662 - -0.8 254,691 + -0.1 254,700 - +0.4 254,702 + -1.6 254,702 + +1.3 254,715 + -0.3 254,738 + -0.1 254,738 + -1.4 254,738 + -1.3 254,738 + +0.4 254,738 + -1.1 254,738 + +0.6 254,738 + +0.4 254,738 + -1.2 254,738 + -0.6 254,738 + -0.5 254,746 - -0.6 254,746 - -1.2 254,746 - +0.9 254,746 - -1.3 254,756 + +0.3 254,756 + -0.3 254,756 + -0.5 254,756 + +0.2 254,756 - +1.2 254,764 - -0.4 254,764 - +0.2 254,764 - -1.3 254,764 - +0.0 254,769 + +0.1 254,774 + +1.3 254,774 + +0.7 254,777 - +0.4 254,777 - -0.8 254,779 + -0.2 254,779 + -0.5 254,782 - +0.3 254,787 - -1.2 254,787 - +0.4 254,826 + GFF250 0.10 +1.9 254,839 - GFF250 0.13 +1.3 254,841 + GFF250 0.13 +0.1 254,841 + GFF250 0.13 -0.1 254,898 + GFF250 0.24 +0.5 254,898 + GFF250 0.24 -1.0 254,898 + GFF250 0.24 -0.6 254,903 + GFF250 0.25 -1.2 254,903 + GFF250 0.25 -0.2 254,904 + GFF250 0.25 -1.9 254,906 - GFF250 0.25 +0.1 254,907 + GFF250 0.25 +0.6 254,923 + GFF250 0.28 -1.2 254,928 + GFF250 0.29 +1.4 254,931 - GFF250 0.30 -2.1 254,938 + GFF250 0.31 +0.4 254,946 - GFF250 0.33 +0.8 254,954 + GFF250 0.34 -1.6 254,954 + GFF250 0.34 -0.8 254,956 + GFF250 0.35 -0.5 254,962 - GFF250 0.36 -0.7 254,962 - GFF250 0.36 -1.0 254,964 - GFF250 0.36 -1.5 254,972 - GFF250 0.38 -0.4 254,972 - GFF250 0.38 -3.3 254,972 - GFF250 0.38 -0.4 254,972 - GFF250 0.38 -1.5 254,972 - GFF250 0.38 -0.4 254,987 - GFF250 0.40 +0.3 254,989 + GFF250 0.41 +0.5 254,994 + GFF250 0.42 -1.8 255,037 + GFF250 0.50 -0.8 255,037 + GFF250 0.50 +1.0 255,038 + GFF250 0.50 -1.3 255,056 + GFF250 0.53 +0.8 255,086 - GFF250 0.59 -0.2 255,094 + GFF250 0.61 -0.7 255,094 + GFF250 0.61 +0.5 255,102 - GFF250 0.62 +1.4 255,103 + GFF250 0.62 +0.0 255,105 + GFF250 0.63 +0.1 255,122 + GFF250 0.66 +0.1 255,123 - GFF250 0.66 -0.4 255,123 - GFF250 0.66 +0.6 255,138 + GFF250 0.69 +0.3 255,140 + GFF250 0.69 -1.7 255,146 - GFF250 0.70 +1.1 255,153 - GFF250 0.72 -0.4 255,159 - GFF250 0.73 -1.2 255,159 - GFF250 0.73 -0.4 255,209 + GFF250 0.82 -0.8 255,244 + GFF250 0.89 -1.2 255,252 - +2.7 255,254 + -2.3 255,254 + +0.0 255,262 - -1.1 255,262 - -0.5 255,262 - -1.3 255,262 - -0.1 255,262 - -1.4 255,262 - -0.7 255,262 - -0.9 255,267 - +0.7 255,270 + -0.0 255,278 - +0.6 255,283 - +0.3 255,296 + -0.4 255,317 + +1.0 255,375 + +0.4 255,397 + GFF251 0.11 -1.3 255,441 - GFF251 0.15 +0.4 255,445 + GFF251 0.16 -1.8 255,470 - GFF251 0.19 -1.6 255,560 + GFF251 0.29 -1.2 255,597 + GFF251 0.33 -0.2 255,618 - GFF251 0.35 +0.6 255,635 + GFF251 0.37 -0.1 255,648 - GFF251 0.38 -1.9 255,673 - GFF251 0.41 +0.2 255,707 + GFF251 0.45 +0.4 255,745 + GFF251 0.49 +1.0 255,770 + GFF251 0.52 -0.8 255,810 + GFF251 0.56 -0.4 255,823 - GFF251 0.58 +2.1 255,833 - GFF251 0.59 +0.1 255,852 - GFF251 0.61 +0.2 255,862 - GFF251 0.62 +2.1 255,892 - GFF251 0.65 +0.6 255,902 - GFF251 0.66 -0.2 255,961 + GFF251 0.73 -0.6 255,984 + GFF251 0.76 -0.3 255,984 + GFF251 0.76 -0.2 255,984 + GFF251 0.76 +0.2 256,030 - GFF251 0.81 -1.4 256,035 - GFF251 0.81 -0.8 256,040 - GFF251 0.82 +0.5 256,053 - GFF251 0.83 +0.7 256,064 + GFF251 0.84 -0.4 256,093 - GFF251 0.88 -1.4 256,097 + GFF251 0.88 +1.7 256,103 + GFF251 0.89 -0.2 256,106 + GFF251 0.89 -1.8 256,114 - +0.1 256,124 - +0.1 256,128 + +0.4 256,133 + -0.6 256,138 + -1.4 256,187 + +0.1 256,195 - -0.6 256,206 - +0.7 256,217 - -0.9 256,229 - -0.8 256,239 - +1.3 256,239 - -1.2 256,241 + -0.1 256,241 + -0.5 256,249 - +0.7 256,249 - +0.4 256,256 + -0.5 256,272 + +0.4 256,277 + -2.6 256,280 - +0.5 256,290 + -1.6 256,297 + -0.2 256,298 - +0.4 256,298 - +1.6 256,315 - GFF252 0.12 -1.2 256,358 - GFF252 0.27 -0.7 256,359 - GFF252 0.27 +1.3 256,423 - GFF252 0.49 +0.3 256,449 + GFF252 0.58 +0.2 256,463 + GFF252 0.63 +0.1 256,473 + GFF252 0.66 +0.6 256,473 + GFF252 0.66 +0.3 256,482 + GFF252 0.69 -0.1 256,482 + GFF252 0.69 -0.9 256,482 + GFF252 0.69 -0.4 256,482 + GFF252 0.69 +0.1 256,482 + GFF252 0.69 +0.4 256,483 + GFF252 0.69 +0.3 256,483 + GFF252 0.69 -0.5 256,490 - GFF252 0.72 -0.3 256,490 - GFF252 0.72 -0.2 256,490 - GFF252 0.72 -1.4 256,490 - GFF252 0.72 +0.0 256,491 - GFF252 0.72 -0.7 256,507 + GFF252 0.78 -0.2 256,509 - GFF252 0.78 -0.1 256,519 - GFF252 0.82 -1.8 256,532 + GFF252 0.86 +1.0 256,532 - GFF252 0.86 +0.9 256,539 - GFF252 0.88 -0.8 256,539 - GFF252 0.88 +0.9 256,539 - GFF252 0.88 -1.2 256,540 - GFF252 0.89 +1.9 256,540 - GFF252 0.89 -1.5 256,541 + GFF252 0.89 +0.8 256,549 - +1.4 256,549 - -1.7 256,576 + +0.2 256,576 + +1.0 256,584 - -0.7 256,584 - -0.3 256,584 - +1.1 256,584 - -0.5 256,584 - -0.2 256,584 - +0.8 256,608 + GFF253 0.21 +0.1 256,616 - GFF253 0.26 +0.7 256,616 - GFF253 0.26 +1.7 256,618 + GFF253 0.28 +1.5 256,618 + GFF253 0.28 -0.3 256,618 + GFF253 0.28 +0.5 256,618 + GFF253 0.28 -0.1 256,618 + GFF253 0.28 +0.4 256,621 - GFF253 0.30 +1.2 256,621 - GFF253 0.30 -1.0 256,621 - GFF253 0.30 +0.1 256,621 - GFF253 0.30 +0.5 256,623 + GFF253 0.31 -0.0 256,623 + GFF253 0.31 -0.8 256,626 - GFF253 0.33 +0.2 256,626 - GFF253 0.33 -0.5 256,626 - GFF253 0.33 -0.8 256,626 - GFF253 0.33 +1.3 256,706 + -0.1 256,706 + +2.1 256,706 + -0.6 256,706 + -0.3 256,706 + +0.4 256,706 + +1.2 256,711 + -0.5 256,714 - -0.2 256,714 - -1.3 256,714 - +0.8 256,716 + -0.7 256,716 + -0.1 256,719 - -1.8 256,748 + -3.3 256,748 + -1.3 256,804 + +1.9 256,874 + -0.5 256,874 + +0.4 256,882 - +0.6 256,892 + -1.3 256,893 + +1.1 256,900 - +0.2 256,900 - +0.6 256,901 + +0.2 256,901 + +0.2 256,907 - -0.8 256,909 - -0.8 256,929 + +0.6 256,929 + -0.1 256,929 + +1.9 256,934 + +1.0 256,942 - -0.5 256,946 + +0.6 256,946 + -0.6 256,953 + +0.4 256,954 - -0.7 257,031 - GFF254 0.13 -0.3 257,041 - GFF254 0.14 +0.7 257,046 + GFF254 0.14 +1.4 257,054 - GFF254 0.15 +1.4 257,054 - GFF254 0.15 -0.6 257,091 + GFF254 0.17 -0.2 257,114 - GFF254 0.18 -2.0 257,139 - GFF254 0.20 -0.9 257,139 - GFF254 0.20 +0.1 257,139 - GFF254 0.20 -0.1 257,175 + GFF254 0.22 +0.5 257,181 + GFF254 0.22 +0.1 257,193 - GFF254 0.23 +0.8
Or see this region's nucleotide sequence