Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF215 and GFF216 are separated by 83 nucleotides GFF216 and GFF217 overlap by 4 nucleotides GFF217 and GFF218 overlap by 4 nucleotides
GFF215 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), C-terminal domain, at 220,743 to 223,346
GFF215
GFF216 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), N-terminal domain, at 223,430 to 224,476
GFF216
GFF217 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 224,473 to 225,405
GFF217
GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373
GFF218
Position (kb)
223
224
225 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 222.430 kb on + strand, within GFF215 at 222.438 kb on - strand, within GFF215 at 222.440 kb on - strand, within GFF215 at 222.440 kb on - strand, within GFF215 at 222.471 kb on - strand, within GFF215 at 222.522 kb on + strand, within GFF215 at 222.528 kb on - strand, within GFF215 at 222.530 kb on + strand, within GFF215 at 222.530 kb on - strand, within GFF215 at 222.530 kb on - strand, within GFF215 at 222.549 kb on + strand, within GFF215 at 222.562 kb on - strand, within GFF215 at 222.577 kb on + strand, within GFF215 at 222.587 kb on + strand, within GFF215 at 222.587 kb on + strand, within GFF215 at 222.592 kb on + strand, within GFF215 at 222.592 kb on + strand, within GFF215 at 222.600 kb on - strand, within GFF215 at 222.600 kb on - strand, within GFF215 at 222.622 kb on + strand, within GFF215 at 222.626 kb on - strand, within GFF215 at 222.630 kb on - strand, within GFF215 at 222.636 kb on - strand, within GFF215 at 222.638 kb on + strand, within GFF215 at 222.638 kb on + strand, within GFF215 at 222.696 kb on + strand, within GFF215 at 222.762 kb on - strand, within GFF215 at 222.834 kb on + strand, within GFF215 at 222.856 kb on + strand, within GFF215 at 222.866 kb on + strand, within GFF215 at 222.866 kb on - strand, within GFF215 at 222.895 kb on + strand, within GFF215 at 222.908 kb on - strand, within GFF215 at 222.913 kb on - strand, within GFF215 at 222.934 kb on - strand, within GFF215 at 222.936 kb on + strand, within GFF215 at 222.937 kb on + strand, within GFF215 at 222.957 kb on - strand, within GFF215 at 222.963 kb on - strand, within GFF215 at 222.971 kb on + strand, within GFF215 at 222.979 kb on - strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.004 kb on + strand, within GFF215 at 223.012 kb on - strand, within GFF215 at 223.012 kb on - strand, within GFF215 at 223.012 kb on - strand, within GFF215 at 223.012 kb on - strand, within GFF215 at 223.012 kb on - strand, within GFF215 at 223.026 kb on - strand, within GFF215 at 223.026 kb on - strand, within GFF215 at 223.026 kb on - strand, within GFF215 at 223.047 kb on + strand, within GFF215 at 223.052 kb on + strand, within GFF215 at 223.055 kb on - strand, within GFF215 at 223.055 kb on - strand, within GFF215 at 223.055 kb on - strand, within GFF215 at 223.065 kb on - strand, within GFF215 at 223.097 kb on + strand at 223.105 kb on - strand at 223.110 kb on - strand at 223.115 kb on + strand at 223.164 kb on + strand at 223.172 kb on - strand at 223.172 kb on - strand at 223.182 kb on + strand at 223.182 kb on - strand at 223.246 kb on - strand at 223.255 kb on + strand at 223.263 kb on - strand at 223.314 kb on + strand at 223.325 kb on + strand at 223.335 kb on - strand at 223.340 kb on - strand at 223.363 kb on + strand at 223.363 kb on + strand at 223.381 kb on - strand at 223.381 kb on - strand at 223.403 kb on - strand at 223.414 kb on - strand at 223.458 kb on - strand at 223.458 kb on - strand at 223.458 kb on - strand at 223.469 kb on + strand at 223.506 kb on + strand at 223.509 kb on - strand at 223.509 kb on - strand at 223.509 kb on - strand at 223.509 kb on - strand at 223.509 kb on - strand at 223.509 kb on - strand at 223.563 kb on + strand, within GFF216 at 223.620 kb on - strand, within GFF216 at 223.642 kb on - strand, within GFF216 at 223.671 kb on + strand, within GFF216 at 223.677 kb on + strand, within GFF216 at 223.677 kb on + strand, within GFF216 at 223.684 kb on - strand, within GFF216 at 223.689 kb on - strand, within GFF216 at 223.707 kb on + strand, within GFF216 at 223.727 kb on + strand, within GFF216 at 223.727 kb on + strand, within GFF216 at 223.730 kb on - strand, within GFF216 at 223.732 kb on + strand, within GFF216 at 223.732 kb on + strand, within GFF216 at 223.732 kb on + strand, within GFF216 at 223.732 kb on + strand, within GFF216 at 223.740 kb on - strand, within GFF216 at 223.740 kb on - strand, within GFF216 at 223.740 kb on - strand, within GFF216 at 223.740 kb on - strand, within GFF216 at 223.740 kb on - strand, within GFF216 at 223.751 kb on + strand, within GFF216 at 223.759 kb on - strand, within GFF216 at 223.764 kb on - strand, within GFF216 at 223.764 kb on - strand, within GFF216 at 223.771 kb on + strand, within GFF216 at 223.771 kb on + strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.779 kb on - strand, within GFF216 at 223.831 kb on - strand, within GFF216 at 223.854 kb on - strand, within GFF216 at 223.874 kb on - strand, within GFF216 at 223.926 kb on - strand, within GFF216 at 224.055 kb on - strand, within GFF216 at 224.061 kb on - strand, within GFF216 at 224.186 kb on + strand, within GFF216 at 224.194 kb on - strand, within GFF216 at 224.194 kb on - strand, within GFF216 at 224.244 kb on + strand, within GFF216 at 224.246 kb on - strand, within GFF216 at 224.246 kb on - strand, within GFF216 at 224.274 kb on - strand, within GFF216 at 224.288 kb on - strand, within GFF216 at 224.298 kb on + strand, within GFF216 at 224.304 kb on + strand, within GFF216 at 224.312 kb on - strand, within GFF216 at 224.331 kb on - strand, within GFF216 at 224.353 kb on + strand, within GFF216 at 224.353 kb on + strand, within GFF216 at 224.388 kb on - strand at 224.406 kb on + strand at 224.408 kb on + strand at 224.416 kb on - strand at 224.434 kb on - strand at 224.514 kb on + strand at 224.539 kb on + strand at 224.539 kb on + strand at 224.547 kb on - strand at 224.547 kb on - strand at 224.608 kb on - strand, within GFF217 at 224.666 kb on - strand, within GFF217 at 224.697 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.702 kb on + strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.710 kb on - strand, within GFF217 at 224.730 kb on - strand, within GFF217 at 224.797 kb on - strand, within GFF217 at 224.844 kb on - strand, within GFF217 at 224.863 kb on + strand, within GFF217 at 224.975 kb on + strand, within GFF217 at 224.975 kb on + strand, within GFF217 at 225.065 kb on - strand, within GFF217 at 225.078 kb on - strand, within GFF217 at 225.100 kb on - strand, within GFF217 at 225.110 kb on + strand, within GFF217 at 225.136 kb on + strand, within GFF217 at 225.144 kb on - strand, within GFF217 at 225.188 kb on + strand, within GFF217 at 225.189 kb on + strand, within GFF217 at 225.197 kb on - strand, within GFF217 at 225.222 kb on + strand, within GFF217 at 225.278 kb on + strand, within GFF217 at 225.351 kb on + strand at 225.361 kb on + strand at 225.374 kb on + strand at 225.395 kb on + strand at 225.403 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 222,430 + GFF215 0.65 -3.1 222,438 - GFF215 0.65 -2.4 222,440 - GFF215 0.65 -2.0 222,440 - GFF215 0.65 -2.0 222,471 - GFF215 0.66 -2.5 222,522 + GFF215 0.68 -0.8 222,528 - GFF215 0.69 -0.6 222,530 + GFF215 0.69 -1.2 222,530 - GFF215 0.69 -3.3 222,530 - GFF215 0.69 -2.2 222,549 + GFF215 0.69 -2.3 222,562 - GFF215 0.70 -2.0 222,577 + GFF215 0.70 -3.3 222,587 + GFF215 0.71 -1.7 222,587 + GFF215 0.71 -1.5 222,592 + GFF215 0.71 -2.8 222,592 + GFF215 0.71 -1.3 222,600 - GFF215 0.71 -1.9 222,600 - GFF215 0.71 -0.9 222,622 + GFF215 0.72 -2.0 222,626 - GFF215 0.72 -0.4 222,630 - GFF215 0.72 -3.8 222,636 - GFF215 0.73 -3.7 222,638 + GFF215 0.73 +0.4 222,638 + GFF215 0.73 -2.0 222,696 + GFF215 0.75 -1.8 222,762 - GFF215 0.78 -2.2 222,834 + GFF215 0.80 -1.2 222,856 + GFF215 0.81 -0.8 222,866 + GFF215 0.82 +0.8 222,866 - GFF215 0.82 -2.9 222,895 + GFF215 0.83 -0.9 222,908 - GFF215 0.83 -2.2 222,913 - GFF215 0.83 -1.3 222,934 - GFF215 0.84 -1.5 222,936 + GFF215 0.84 -1.5 222,937 + GFF215 0.84 -1.8 222,957 - GFF215 0.85 -2.0 222,963 - GFF215 0.85 -0.5 222,971 + GFF215 0.86 -2.0 222,979 - GFF215 0.86 -1.8 223,004 + GFF215 0.87 -0.6 223,004 + GFF215 0.87 -2.7 223,004 + GFF215 0.87 -0.9 223,004 + GFF215 0.87 -1.6 223,004 + GFF215 0.87 -2.8 223,004 + GFF215 0.87 -1.2 223,004 + GFF215 0.87 -0.8 223,004 + GFF215 0.87 -2.4 223,004 + GFF215 0.87 -0.2 223,004 + GFF215 0.87 -1.4 223,004 + GFF215 0.87 -1.0 223,004 + GFF215 0.87 -2.9 223,012 - GFF215 0.87 -2.3 223,012 - GFF215 0.87 -1.6 223,012 - GFF215 0.87 -3.0 223,012 - GFF215 0.87 -2.7 223,012 - GFF215 0.87 -1.8 223,026 - GFF215 0.88 -2.2 223,026 - GFF215 0.88 -3.1 223,026 - GFF215 0.88 -2.1 223,047 + GFF215 0.88 -3.3 223,052 + GFF215 0.89 -0.9 223,055 - GFF215 0.89 -2.2 223,055 - GFF215 0.89 -1.8 223,055 - GFF215 0.89 -2.4 223,065 - GFF215 0.89 -1.9 223,097 + -0.8 223,105 - -0.5 223,110 - -1.5 223,115 + -1.4 223,164 + -2.3 223,172 - -1.5 223,172 - -1.9 223,182 + +0.4 223,182 - -2.4 223,246 - -2.5 223,255 + -2.0 223,263 - -0.8 223,314 + -0.4 223,325 + -0.8 223,335 - -2.6 223,340 - -1.0 223,363 + +0.6 223,363 + +1.0 223,381 - +0.0 223,381 - +0.5 223,403 - +0.5 223,414 - +0.6 223,458 - -1.0 223,458 - -0.9 223,458 - +0.2 223,469 + -0.9 223,506 + -1.0 223,509 - -1.7 223,509 - -2.5 223,509 - -2.6 223,509 - -1.0 223,509 - -2.1 223,509 - -1.3 223,563 + GFF216 0.13 -2.6 223,620 - GFF216 0.18 -1.6 223,642 - GFF216 0.20 -0.7 223,671 + GFF216 0.23 -1.9 223,677 + GFF216 0.24 -1.0 223,677 + GFF216 0.24 -3.1 223,684 - GFF216 0.24 -1.2 223,689 - GFF216 0.25 -0.6 223,707 + GFF216 0.26 -2.3 223,727 + GFF216 0.28 -2.0 223,727 + GFF216 0.28 -2.5 223,730 - GFF216 0.29 +0.4 223,732 + GFF216 0.29 -2.1 223,732 + GFF216 0.29 -1.8 223,732 + GFF216 0.29 -1.4 223,732 + GFF216 0.29 -1.5 223,740 - GFF216 0.30 -1.0 223,740 - GFF216 0.30 -1.8 223,740 - GFF216 0.30 -1.2 223,740 - GFF216 0.30 -1.2 223,740 - GFF216 0.30 -1.8 223,751 + GFF216 0.31 -1.1 223,759 - GFF216 0.31 -2.4 223,764 - GFF216 0.32 -0.9 223,764 - GFF216 0.32 -1.5 223,771 + GFF216 0.33 -2.6 223,771 + GFF216 0.33 -2.8 223,779 - GFF216 0.33 +0.1 223,779 - GFF216 0.33 -2.3 223,779 - GFF216 0.33 +0.0 223,779 - GFF216 0.33 -0.3 223,779 - GFF216 0.33 -1.4 223,779 - GFF216 0.33 -2.0 223,831 - GFF216 0.38 -1.7 223,854 - GFF216 0.40 -2.0 223,874 - GFF216 0.42 -1.7 223,926 - GFF216 0.47 -2.9 224,055 - GFF216 0.60 -1.0 224,061 - GFF216 0.60 -2.3 224,186 + GFF216 0.72 -1.3 224,194 - GFF216 0.73 -1.4 224,194 - GFF216 0.73 +0.4 224,244 + GFF216 0.78 -0.6 224,246 - GFF216 0.78 -1.0 224,246 - GFF216 0.78 -1.5 224,274 - GFF216 0.81 -0.2 224,288 - GFF216 0.82 -2.8 224,298 + GFF216 0.83 -2.1 224,304 + GFF216 0.83 -2.4 224,312 - GFF216 0.84 -0.8 224,331 - GFF216 0.86 -0.9 224,353 + GFF216 0.88 -1.5 224,353 + GFF216 0.88 -2.0 224,388 - -0.2 224,406 + -3.1 224,408 + -0.2 224,416 - -2.2 224,434 - -3.3 224,514 + -2.0 224,539 + -2.0 224,539 + -2.1 224,547 - -1.3 224,547 - -1.8 224,608 - GFF217 0.14 -2.7 224,666 - GFF217 0.21 -3.7 224,697 + GFF217 0.24 -2.5 224,702 + GFF217 0.25 -2.8 224,702 + GFF217 0.25 -3.4 224,702 + GFF217 0.25 -1.6 224,702 + GFF217 0.25 -0.2 224,702 + GFF217 0.25 -3.4 224,702 + GFF217 0.25 -2.8 224,710 - GFF217 0.25 -0.8 224,710 - GFF217 0.25 -2.4 224,710 - GFF217 0.25 -2.5 224,730 - GFF217 0.28 -0.9 224,797 - GFF217 0.35 -2.5 224,844 - GFF217 0.40 -1.7 224,863 + GFF217 0.42 -2.0 224,975 + GFF217 0.54 -3.7 224,975 + GFF217 0.54 -0.7 225,065 - GFF217 0.63 -2.0 225,078 - GFF217 0.65 -2.0 225,100 - GFF217 0.67 -2.3 225,110 + GFF217 0.68 +0.7 225,136 + GFF217 0.71 -1.4 225,144 - GFF217 0.72 -3.2 225,188 + GFF217 0.77 -3.4 225,189 + GFF217 0.77 -2.3 225,197 - GFF217 0.78 -2.2 225,222 + GFF217 0.80 -2.7 225,278 + GFF217 0.86 -2.6 225,351 + -1.3 225,361 + -1.4 225,374 + -0.2 225,395 + -0.7 225,403 - -0.5
Or see this region's nucleotide sequence