Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1850 and GFF1851 are separated by 161 nucleotides GFF1851 and GFF1852 overlap by 1 nucleotides GFF1852 and GFF1853 are separated by 2 nucleotides GFF1853 and GFF1854 are separated by 2 nucleotides
GFF1850 - Transcriptional regulator, AcrR family, at 1,880,997 to 1,881,668
GFF1850
GFF1851 - Precorrin-6A synthase (deacetylating) (EC 2.1.1.152), at 1,881,830 to 1,882,582
GFF1851
GFF1852 - Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing) (EC 6.3.5.9), at 1,882,582 to 1,883,880
GFF1852
GFF1853 - Precorrin-4 C(11)-methyltransferase (EC 2.1.1.133), at 1,883,883 to 1,884,668
GFF1853
GFF1854 - Cobalamin biosynthesis protein CobE, at 1,884,671 to 1,885,027
GFF1854
Position (kb)
1882
1883
1884 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1881.582 kb on + strand, within GFF1850 at 1881.628 kb on + strand at 1881.661 kb on - strand at 1881.733 kb on + strand at 1881.769 kb on + strand at 1881.845 kb on + strand at 1881.914 kb on - strand, within GFF1851 at 1881.914 kb on - strand, within GFF1851 at 1882.022 kb on - strand, within GFF1851 at 1882.022 kb on - strand, within GFF1851 at 1882.027 kb on - strand, within GFF1851 at 1882.078 kb on + strand, within GFF1851 at 1882.097 kb on - strand, within GFF1851 at 1882.130 kb on + strand, within GFF1851 at 1882.130 kb on + strand, within GFF1851 at 1882.138 kb on - strand, within GFF1851 at 1882.138 kb on - strand, within GFF1851 at 1882.179 kb on - strand, within GFF1851 at 1882.208 kb on - strand, within GFF1851 at 1882.235 kb on + strand, within GFF1851 at 1882.237 kb on + strand, within GFF1851 at 1882.254 kb on + strand, within GFF1851 at 1882.254 kb on + strand, within GFF1851 at 1882.254 kb on + strand, within GFF1851 at 1882.254 kb on + strand, within GFF1851 at 1882.254 kb on + strand, within GFF1851 at 1882.259 kb on + strand, within GFF1851 at 1882.262 kb on + strand, within GFF1851 at 1882.262 kb on + strand, within GFF1851 at 1882.262 kb on - strand, within GFF1851 at 1882.262 kb on - strand, within GFF1851 at 1882.275 kb on - strand, within GFF1851 at 1882.275 kb on - strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.311 kb on + strand, within GFF1851 at 1882.314 kb on - strand, within GFF1851 at 1882.314 kb on - strand, within GFF1851 at 1882.316 kb on + strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.319 kb on - strand, within GFF1851 at 1882.340 kb on + strand, within GFF1851 at 1882.348 kb on - strand, within GFF1851 at 1882.348 kb on - strand, within GFF1851 at 1882.387 kb on + strand, within GFF1851 at 1882.480 kb on - strand, within GFF1851 at 1882.489 kb on + strand, within GFF1851 at 1882.527 kb on - strand at 1882.528 kb on - strand at 1882.567 kb on + strand at 1882.567 kb on + strand at 1882.578 kb on + strand at 1882.681 kb on - strand at 1882.701 kb on - strand at 1882.792 kb on + strand, within GFF1852 at 1882.846 kb on - strand, within GFF1852 at 1882.859 kb on + strand, within GFF1852 at 1882.859 kb on + strand, within GFF1852 at 1882.859 kb on + strand, within GFF1852 at 1882.893 kb on - strand, within GFF1852 at 1882.893 kb on - strand, within GFF1852 at 1882.893 kb on - strand, within GFF1852 at 1882.893 kb on - strand, within GFF1852 at 1882.893 kb on - strand, within GFF1852 at 1882.901 kb on - strand, within GFF1852 at 1882.922 kb on + strand, within GFF1852 at 1882.927 kb on + strand, within GFF1852 at 1882.927 kb on + strand, within GFF1852 at 1882.927 kb on + strand, within GFF1852 at 1882.927 kb on + strand, within GFF1852 at 1882.935 kb on - strand, within GFF1852 at 1882.935 kb on - strand, within GFF1852 at 1882.935 kb on - strand, within GFF1852 at 1883.066 kb on + strand, within GFF1852 at 1883.096 kb on - strand, within GFF1852 at 1883.296 kb on + strand, within GFF1852 at 1883.296 kb on + strand, within GFF1852 at 1883.324 kb on + strand, within GFF1852 at 1883.335 kb on - strand, within GFF1852 at 1883.352 kb on + strand, within GFF1852 at 1883.497 kb on + strand, within GFF1852 at 1883.497 kb on + strand, within GFF1852 at 1883.497 kb on + strand, within GFF1852 at 1883.527 kb on + strand, within GFF1852 at 1883.527 kb on + strand, within GFF1852 at 1883.551 kb on - strand, within GFF1852 at 1883.551 kb on - strand, within GFF1852 at 1883.603 kb on + strand, within GFF1852 at 1883.603 kb on - strand, within GFF1852 at 1883.614 kb on + strand, within GFF1852 at 1883.671 kb on + strand, within GFF1852 at 1883.671 kb on + strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.679 kb on - strand, within GFF1852 at 1883.688 kb on + strand, within GFF1852 at 1883.706 kb on + strand, within GFF1852 at 1883.748 kb on + strand, within GFF1852 at 1883.753 kb on + strand at 1883.785 kb on + strand at 1883.789 kb on - strand at 1883.798 kb on - strand at 1883.807 kb on + strand at 1883.894 kb on + strand at 1883.894 kb on + strand at 1883.902 kb on - strand at 1883.907 kb on - strand at 1883.921 kb on - strand at 1883.937 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.938 kb on + strand at 1883.944 kb on - strand at 1883.946 kb on - strand at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.017 kb on + strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.040 kb on + strand, within GFF1853 at 1884.077 kb on + strand, within GFF1853 at 1884.085 kb on - strand, within GFF1853 at 1884.112 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.153 kb on - strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.248 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.284 kb on + strand, within GFF1853 at 1884.314 kb on - strand, within GFF1853 at 1884.437 kb on + strand, within GFF1853 at 1884.439 kb on + strand, within GFF1853 at 1884.487 kb on + strand, within GFF1853 at 1884.494 kb on + strand, within GFF1853 at 1884.508 kb on + strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.541 kb on - strand, within GFF1853 at 1884.579 kb on - strand, within GFF1853 at 1884.677 kb on + strand at 1884.678 kb on + strand at 1884.678 kb on - strand at 1884.719 kb on + strand, within GFF1854 at 1884.727 kb on - strand, within GFF1854 at 1884.765 kb on + strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.784 kb on + strand, within GFF1854 at 1884.828 kb on - strand, within GFF1854 at 1884.838 kb on - strand, within GFF1854
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,881,582 + GFF1850 0.87 -2.6 1,881,628 + +0.8 1,881,661 - +0.5 1,881,733 + +0.4 1,881,769 + +0.8 1,881,845 + +0.4 1,881,914 - GFF1851 0.11 -2.0 1,881,914 - GFF1851 0.11 -0.2 1,882,022 - GFF1851 0.25 -1.6 1,882,022 - GFF1851 0.25 -0.9 1,882,027 - GFF1851 0.26 +1.1 1,882,078 + GFF1851 0.33 -2.2 1,882,097 - GFF1851 0.35 -0.2 1,882,130 + GFF1851 0.40 -1.8 1,882,130 + GFF1851 0.40 -2.1 1,882,138 - GFF1851 0.41 -1.6 1,882,138 - GFF1851 0.41 -0.4 1,882,179 - GFF1851 0.46 -1.5 1,882,208 - GFF1851 0.50 -1.8 1,882,235 + GFF1851 0.54 -1.0 1,882,237 + GFF1851 0.54 -2.1 1,882,254 + GFF1851 0.56 -0.9 1,882,254 + GFF1851 0.56 -1.3 1,882,254 + GFF1851 0.56 +0.1 1,882,254 + GFF1851 0.56 -3.6 1,882,254 + GFF1851 0.56 -2.0 1,882,259 + GFF1851 0.57 -2.0 1,882,262 + GFF1851 0.57 -0.7 1,882,262 + GFF1851 0.57 -1.7 1,882,262 - GFF1851 0.57 -0.9 1,882,262 - GFF1851 0.57 -0.9 1,882,275 - GFF1851 0.59 -0.7 1,882,275 - GFF1851 0.59 -0.9 1,882,311 + GFF1851 0.64 -2.1 1,882,311 + GFF1851 0.64 -0.7 1,882,311 + GFF1851 0.64 -0.5 1,882,311 + GFF1851 0.64 +0.1 1,882,311 + GFF1851 0.64 -0.2 1,882,311 + GFF1851 0.64 +0.4 1,882,311 + GFF1851 0.64 -1.6 1,882,314 - GFF1851 0.64 -0.9 1,882,314 - GFF1851 0.64 -3.4 1,882,316 + GFF1851 0.65 -0.7 1,882,319 - GFF1851 0.65 -0.9 1,882,319 - GFF1851 0.65 +0.1 1,882,319 - GFF1851 0.65 -0.7 1,882,319 - GFF1851 0.65 -0.4 1,882,319 - GFF1851 0.65 -0.6 1,882,319 - GFF1851 0.65 -0.7 1,882,319 - GFF1851 0.65 -0.4 1,882,319 - GFF1851 0.65 -2.2 1,882,319 - GFF1851 0.65 +0.4 1,882,319 - GFF1851 0.65 -2.2 1,882,319 - GFF1851 0.65 -0.4 1,882,340 + GFF1851 0.68 -2.4 1,882,348 - GFF1851 0.69 -0.7 1,882,348 - GFF1851 0.69 -1.0 1,882,387 + GFF1851 0.74 +0.1 1,882,480 - GFF1851 0.86 -0.2 1,882,489 + GFF1851 0.88 -0.7 1,882,527 - -2.0 1,882,528 - -1.2 1,882,567 + -1.0 1,882,567 + -0.7 1,882,578 + -0.0 1,882,681 - -1.7 1,882,701 - -1.9 1,882,792 + GFF1852 0.16 -2.3 1,882,846 - GFF1852 0.20 -0.2 1,882,859 + GFF1852 0.21 +0.0 1,882,859 + GFF1852 0.21 -0.0 1,882,859 + GFF1852 0.21 -1.9 1,882,893 - GFF1852 0.24 -1.7 1,882,893 - GFF1852 0.24 -0.9 1,882,893 - GFF1852 0.24 -1.8 1,882,893 - GFF1852 0.24 +0.4 1,882,893 - GFF1852 0.24 -2.0 1,882,901 - GFF1852 0.25 +0.4 1,882,922 + GFF1852 0.26 -0.2 1,882,927 + GFF1852 0.27 -0.5 1,882,927 + GFF1852 0.27 -1.3 1,882,927 + GFF1852 0.27 -1.4 1,882,927 + GFF1852 0.27 -0.6 1,882,935 - GFF1852 0.27 -1.7 1,882,935 - GFF1852 0.27 -1.9 1,882,935 - GFF1852 0.27 +2.8 1,883,066 + GFF1852 0.37 -1.2 1,883,096 - GFF1852 0.40 -1.9 1,883,296 + GFF1852 0.55 -0.6 1,883,296 + GFF1852 0.55 -1.2 1,883,324 + GFF1852 0.57 -1.7 1,883,335 - GFF1852 0.58 -0.8 1,883,352 + GFF1852 0.59 -0.9 1,883,497 + GFF1852 0.70 +0.4 1,883,497 + GFF1852 0.70 -0.2 1,883,497 + GFF1852 0.70 -0.9 1,883,527 + GFF1852 0.73 -0.2 1,883,527 + GFF1852 0.73 -1.3 1,883,551 - GFF1852 0.75 -0.2 1,883,551 - GFF1852 0.75 -1.4 1,883,603 + GFF1852 0.79 -1.5 1,883,603 - GFF1852 0.79 +1.0 1,883,614 + GFF1852 0.79 -0.7 1,883,671 + GFF1852 0.84 -1.4 1,883,671 + GFF1852 0.84 -1.0 1,883,679 - GFF1852 0.84 -2.6 1,883,679 - GFF1852 0.84 -2.6 1,883,679 - GFF1852 0.84 -0.4 1,883,688 + GFF1852 0.85 -1.0 1,883,706 + GFF1852 0.87 -1.8 1,883,748 + GFF1852 0.90 -1.0 1,883,753 + -1.7 1,883,785 + -0.3 1,883,789 - -1.7 1,883,798 - -0.7 1,883,807 + +2.7 1,883,894 + +0.6 1,883,894 + +0.8 1,883,902 - +0.0 1,883,907 - +2.4 1,883,921 - +1.1 1,883,937 + +0.1 1,883,938 + +1.2 1,883,938 + +0.4 1,883,938 + -0.6 1,883,944 - +0.4 1,883,946 - -1.9 1,884,017 + GFF1853 0.17 -2.1 1,884,017 + GFF1853 0.17 +0.1 1,884,017 + GFF1853 0.17 -3.2 1,884,017 + GFF1853 0.17 +0.6 1,884,025 - GFF1853 0.18 -0.9 1,884,025 - GFF1853 0.18 -2.0 1,884,025 - GFF1853 0.18 -1.8 1,884,025 - GFF1853 0.18 -1.5 1,884,040 + GFF1853 0.20 -1.5 1,884,077 + GFF1853 0.25 +1.1 1,884,085 - GFF1853 0.26 -1.3 1,884,112 + GFF1853 0.29 -1.2 1,884,145 + GFF1853 0.33 -1.6 1,884,145 + GFF1853 0.33 +0.4 1,884,145 + GFF1853 0.33 +0.4 1,884,153 - GFF1853 0.34 -1.3 1,884,183 + GFF1853 0.38 -0.2 1,884,183 + GFF1853 0.38 -0.4 1,884,238 + GFF1853 0.45 -0.6 1,884,238 + GFF1853 0.45 -1.5 1,884,238 + GFF1853 0.45 -1.8 1,884,238 + GFF1853 0.45 -0.7 1,884,238 + GFF1853 0.45 -1.7 1,884,238 + GFF1853 0.45 -1.2 1,884,246 - GFF1853 0.46 -2.2 1,884,246 - GFF1853 0.46 -2.2 1,884,246 - GFF1853 0.46 -1.4 1,884,248 + GFF1853 0.46 -1.4 1,884,283 + GFF1853 0.51 -0.7 1,884,283 + GFF1853 0.51 -1.5 1,884,284 + GFF1853 0.51 +0.4 1,884,314 - GFF1853 0.55 -1.6 1,884,437 + GFF1853 0.70 -1.8 1,884,439 + GFF1853 0.71 -0.9 1,884,487 + GFF1853 0.77 -1.7 1,884,494 + GFF1853 0.78 +0.7 1,884,508 + GFF1853 0.80 -3.0 1,884,516 - GFF1853 0.81 -1.7 1,884,541 - GFF1853 0.84 -2.0 1,884,579 - GFF1853 0.89 -1.2 1,884,677 + -1.0 1,884,678 + -0.3 1,884,678 - +0.5 1,884,719 + GFF1854 0.13 -0.7 1,884,727 - GFF1854 0.16 -2.0 1,884,765 + GFF1854 0.26 -1.6 1,884,771 - GFF1854 0.28 -1.4 1,884,771 - GFF1854 0.28 -0.7 1,884,784 + GFF1854 0.32 +0.3 1,884,828 - GFF1854 0.44 -1.7 1,884,838 - GFF1854 0.47 -0.7
Or see this region's nucleotide sequence