Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1573 and GFF1574 are separated by 84 nucleotides GFF1574 and GFF1575 overlap by 4 nucleotides GFF1575 and GFF1576 are separated by 157 nucleotides
GFF1573 - Enoyl-CoA hydratase (EC 4.2.1.17) => degradation of branched-chain amino acids and alpha-keto acids, at 1,602,112 to 1,602,888
GFF1573
GFF1574 - FolM Alternative dihydrofolate reductase 1, at 1,602,973 to 1,603,833
GFF1574
GFF1575 - GTP cyclohydrolase I (EC 3.5.4.16) type 1, at 1,603,830 to 1,604,438
GFF1575
GFF1576 - Molybdenum cofactor cytidylyltransferase (EC 2.7.7.76), at 1,604,596 to 1,605,192
GFF1576
Position (kb)
1602
1603
1604 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1602.050 kb on + strand at 1602.055 kb on + strand at 1602.055 kb on + strand at 1602.060 kb on + strand at 1602.063 kb on - strand at 1602.191 kb on + strand, within GFF1573 at 1602.225 kb on + strand, within GFF1573 at 1602.311 kb on + strand, within GFF1573 at 1602.422 kb on + strand, within GFF1573 at 1602.430 kb on - strand, within GFF1573 at 1602.472 kb on - strand, within GFF1573 at 1602.492 kb on + strand, within GFF1573 at 1602.499 kb on - strand, within GFF1573 at 1602.536 kb on - strand, within GFF1573 at 1602.585 kb on - strand, within GFF1573 at 1602.585 kb on - strand, within GFF1573 at 1602.724 kb on + strand, within GFF1573 at 1602.724 kb on + strand, within GFF1573 at 1602.724 kb on + strand, within GFF1573 at 1602.734 kb on + strand, within GFF1573 at 1602.734 kb on + strand, within GFF1573 at 1602.792 kb on + strand, within GFF1573 at 1602.904 kb on - strand at 1602.909 kb on - strand at 1602.915 kb on + strand at 1602.999 kb on + strand at 1603.039 kb on + strand at 1603.047 kb on - strand at 1603.047 kb on - strand at 1603.047 kb on - strand at 1603.185 kb on - strand, within GFF1574 at 1603.190 kb on - strand, within GFF1574 at 1603.217 kb on - strand, within GFF1574 at 1603.244 kb on + strand, within GFF1574 at 1603.269 kb on + strand, within GFF1574 at 1603.269 kb on + strand, within GFF1574 at 1603.271 kb on + strand, within GFF1574 at 1603.271 kb on + strand, within GFF1574 at 1603.271 kb on + strand, within GFF1574 at 1603.271 kb on + strand, within GFF1574 at 1603.372 kb on + strand, within GFF1574 at 1603.399 kb on - strand, within GFF1574 at 1603.442 kb on + strand, within GFF1574 at 1603.469 kb on + strand, within GFF1574 at 1603.469 kb on + strand, within GFF1574 at 1603.469 kb on + strand, within GFF1574 at 1603.477 kb on - strand, within GFF1574 at 1603.639 kb on - strand, within GFF1574 at 1603.641 kb on - strand, within GFF1574 at 1603.666 kb on - strand, within GFF1574 at 1603.763 kb on + strand at 1603.804 kb on + strand at 1603.817 kb on - strand at 1604.442 kb on - strand at 1604.447 kb on - strand at 1604.447 kb on - strand at 1604.460 kb on - strand at 1604.493 kb on - strand at 1604.495 kb on + strand at 1604.514 kb on + strand at 1604.517 kb on + strand at 1604.517 kb on + strand at 1604.530 kb on - strand at 1604.532 kb on - strand at 1604.593 kb on + strand at 1604.630 kb on - strand at 1604.630 kb on - strand at 1604.640 kb on - strand at 1604.667 kb on + strand, within GFF1576 at 1604.680 kb on - strand, within GFF1576 at 1604.754 kb on + strand, within GFF1576 at 1604.792 kb on + strand, within GFF1576 at 1604.800 kb on - strand, within GFF1576 at 1604.800 kb on - strand, within GFF1576 at 1604.815 kb on - strand, within GFF1576
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,602,050 + +0.2 1,602,055 + -0.0 1,602,055 + -0.5 1,602,060 + -1.1 1,602,063 - +0.5 1,602,191 + GFF1573 0.10 -0.0 1,602,225 + GFF1573 0.15 -1.1 1,602,311 + GFF1573 0.26 -1.0 1,602,422 + GFF1573 0.40 +1.1 1,602,430 - GFF1573 0.41 +0.3 1,602,472 - GFF1573 0.46 -1.6 1,602,492 + GFF1573 0.49 -0.5 1,602,499 - GFF1573 0.50 +1.1 1,602,536 - GFF1573 0.55 -0.6 1,602,585 - GFF1573 0.61 +1.4 1,602,585 - GFF1573 0.61 -1.3 1,602,724 + GFF1573 0.79 +0.3 1,602,724 + GFF1573 0.79 +1.6 1,602,724 + GFF1573 0.79 -1.6 1,602,734 + GFF1573 0.80 -1.1 1,602,734 + GFF1573 0.80 -0.0 1,602,792 + GFF1573 0.88 -0.4 1,602,904 - -1.1 1,602,909 - +0.1 1,602,915 + -1.3 1,602,999 + +2.4 1,603,039 + -1.6 1,603,047 - +1.3 1,603,047 - -1.6 1,603,047 - +0.1 1,603,185 - GFF1574 0.25 -0.9 1,603,190 - GFF1574 0.25 -1.4 1,603,217 - GFF1574 0.28 +0.1 1,603,244 + GFF1574 0.31 +0.7 1,603,269 + GFF1574 0.34 -3.3 1,603,269 + GFF1574 0.34 -0.7 1,603,271 + GFF1574 0.35 +0.3 1,603,271 + GFF1574 0.35 +0.4 1,603,271 + GFF1574 0.35 -1.3 1,603,271 + GFF1574 0.35 -0.8 1,603,372 + GFF1574 0.46 -2.5 1,603,399 - GFF1574 0.49 -0.9 1,603,442 + GFF1574 0.54 -1.4 1,603,469 + GFF1574 0.58 -1.5 1,603,469 + GFF1574 0.58 -2.1 1,603,469 + GFF1574 0.58 -1.1 1,603,477 - GFF1574 0.59 -0.4 1,603,639 - GFF1574 0.77 +1.0 1,603,641 - GFF1574 0.78 +0.1 1,603,666 - GFF1574 0.80 -1.7 1,603,763 + +0.9 1,603,804 + -0.2 1,603,817 - +0.4 1,604,442 - +0.8 1,604,447 - +1.2 1,604,447 - -1.5 1,604,460 - -0.2 1,604,493 - +0.2 1,604,495 + -0.7 1,604,514 + +0.3 1,604,517 + -0.4 1,604,517 + -0.1 1,604,530 - -1.3 1,604,532 - -0.5 1,604,593 + -0.3 1,604,630 - -1.3 1,604,630 - +1.5 1,604,640 - -1.1 1,604,667 + GFF1576 0.12 +0.2 1,604,680 - GFF1576 0.14 -1.3 1,604,754 + GFF1576 0.26 +1.6 1,604,792 + GFF1576 0.33 +0.2 1,604,800 - GFF1576 0.34 -1.6 1,604,800 - GFF1576 0.34 -0.1 1,604,815 - GFF1576 0.37 -0.5
Or see this region's nucleotide sequence