Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF140 and GFF141 are separated by 190 nucleotides GFF141 and GFF142 are separated by 50 nucleotides
GFF140 - Alanine racemase (EC 5.1.1.1), at 134,624 to 135,667
GFF140
GFF141 - Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 135,858 to 137,030
GFF141
GFF142 - Polyhydroxyalkanoic acid synthase, at 137,081 to 138,055
GFF142
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 135.696 kb on - strand at 135.905 kb on + strand at 135.909 kb on + strand at 135.917 kb on - strand at 135.970 kb on - strand at 136.018 kb on + strand, within GFF141 at 136.026 kb on - strand, within GFF141 at 136.026 kb on - strand, within GFF141 at 136.026 kb on - strand, within GFF141 at 136.163 kb on - strand, within GFF141 at 136.236 kb on + strand, within GFF141 at 136.323 kb on - strand, within GFF141 at 136.402 kb on + strand, within GFF141 at 136.440 kb on - strand, within GFF141 at 136.454 kb on + strand, within GFF141 at 136.471 kb on + strand, within GFF141 at 136.471 kb on + strand, within GFF141 at 136.479 kb on - strand, within GFF141 at 136.485 kb on - strand, within GFF141 at 136.555 kb on + strand, within GFF141 at 136.563 kb on - strand, within GFF141 at 136.572 kb on + strand, within GFF141 at 136.580 kb on - strand, within GFF141 at 136.618 kb on + strand, within GFF141 at 136.632 kb on + strand, within GFF141 at 136.633 kb on + strand, within GFF141 at 136.652 kb on + strand, within GFF141 at 136.666 kb on - strand, within GFF141 at 136.728 kb on - strand, within GFF141 at 136.757 kb on + strand, within GFF141 at 136.757 kb on + strand, within GFF141 at 136.760 kb on - strand, within GFF141 at 136.765 kb on - strand, within GFF141 at 136.765 kb on - strand, within GFF141 at 136.765 kb on - strand, within GFF141 at 136.775 kb on + strand, within GFF141 at 136.829 kb on + strand, within GFF141 at 136.839 kb on + strand, within GFF141 at 136.839 kb on + strand, within GFF141 at 136.839 kb on + strand, within GFF141 at 136.839 kb on + strand, within GFF141 at 136.839 kb on + strand, within GFF141 at 136.847 kb on - strand, within GFF141 at 136.847 kb on - strand, within GFF141 at 136.847 kb on - strand, within GFF141 at 136.847 kb on - strand, within GFF141 at 136.858 kb on - strand, within GFF141 at 136.863 kb on + strand, within GFF141 at 136.917 kb on - strand at 137.048 kb on - strand at 137.076 kb on - strand at 137.101 kb on + strand at 137.101 kb on + strand at 137.101 kb on + strand at 137.101 kb on + strand at 137.109 kb on - strand at 137.109 kb on - strand at 137.109 kb on - strand at 137.109 kb on - strand at 137.109 kb on - strand at 137.109 kb on - strand at 137.121 kb on - strand at 137.121 kb on - strand at 137.127 kb on + strand at 137.144 kb on + strand at 137.157 kb on - strand at 137.164 kb on + strand at 137.164 kb on + strand at 137.171 kb on + strand at 137.172 kb on - strand at 137.200 kb on + strand, within GFF142 at 137.203 kb on - strand, within GFF142 at 137.213 kb on - strand, within GFF142 at 137.253 kb on + strand, within GFF142 at 137.256 kb on - strand, within GFF142 at 137.256 kb on - strand, within GFF142 at 137.261 kb on - strand, within GFF142 at 137.261 kb on - strand, within GFF142 at 137.273 kb on + strand, within GFF142 at 137.281 kb on - strand, within GFF142 at 137.363 kb on + strand, within GFF142 at 137.366 kb on - strand, within GFF142 at 137.371 kb on - strand, within GFF142 at 137.371 kb on - strand, within GFF142 at 137.380 kb on + strand, within GFF142 at 137.380 kb on + strand, within GFF142 at 137.383 kb on - strand, within GFF142 at 137.388 kb on - strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.390 kb on + strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.398 kb on - strand, within GFF142 at 137.427 kb on - strand, within GFF142 at 137.427 kb on - strand, within GFF142 at 137.449 kb on - strand, within GFF142 at 137.464 kb on - strand, within GFF142 at 137.464 kb on - strand, within GFF142 at 137.475 kb on + strand, within GFF142 at 137.478 kb on - strand, within GFF142 at 137.492 kb on - strand, within GFF142 at 137.515 kb on + strand, within GFF142 at 137.520 kb on + strand, within GFF142 at 137.523 kb on - strand, within GFF142 at 137.531 kb on + strand, within GFF142 at 137.542 kb on + strand, within GFF142 at 137.550 kb on - strand, within GFF142 at 137.550 kb on - strand, within GFF142 at 137.555 kb on + strand, within GFF142 at 137.555 kb on + strand, within GFF142 at 137.555 kb on + strand, within GFF142 at 137.555 kb on + strand, within GFF142 at 137.555 kb on + strand, within GFF142 at 137.561 kb on - strand, within GFF142 at 137.563 kb on - strand, within GFF142 at 137.563 kb on - strand, within GFF142 at 137.563 kb on - strand, within GFF142 at 137.563 kb on - strand, within GFF142 at 137.568 kb on - strand, within GFF142 at 137.580 kb on - strand, within GFF142 at 137.580 kb on - strand, within GFF142 at 137.587 kb on + strand, within GFF142 at 137.603 kb on + strand, within GFF142 at 137.603 kb on + strand, within GFF142 at 137.611 kb on - strand, within GFF142 at 137.611 kb on - strand, within GFF142 at 137.611 kb on - strand, within GFF142 at 137.632 kb on - strand, within GFF142 at 137.638 kb on - strand, within GFF142 at 137.642 kb on + strand, within GFF142 at 137.642 kb on + strand, within GFF142 at 137.642 kb on + strand, within GFF142 at 137.642 kb on + strand, within GFF142 at 137.642 kb on + strand, within GFF142 at 137.645 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.650 kb on - strand, within GFF142 at 137.678 kb on - strand, within GFF142 at 137.680 kb on + strand, within GFF142 at 137.680 kb on + strand, within GFF142 at 137.680 kb on + strand, within GFF142 at 137.680 kb on + strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.688 kb on - strand, within GFF142 at 137.695 kb on + strand, within GFF142 at 137.703 kb on - strand, within GFF142 at 137.703 kb on - strand, within GFF142 at 137.708 kb on + strand, within GFF142 at 137.716 kb on - strand, within GFF142 at 137.727 kb on + strand, within GFF142 at 137.727 kb on + strand, within GFF142 at 137.727 kb on + strand, within GFF142 at 137.727 kb on + strand, within GFF142 at 137.728 kb on + strand, within GFF142 at 137.728 kb on + strand, within GFF142 at 137.735 kb on - strand, within GFF142 at 137.736 kb on - strand, within GFF142 at 137.746 kb on - strand, within GFF142 at 137.796 kb on - strand, within GFF142 at 137.803 kb on + strand, within GFF142 at 137.834 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.839 kb on + strand, within GFF142 at 137.860 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.861 kb on + strand, within GFF142 at 137.868 kb on - strand, within GFF142 at 137.868 kb on - strand, within GFF142 at 137.869 kb on + strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.869 kb on - strand, within GFF142 at 137.872 kb on + strand, within GFF142 at 137.872 kb on + strand, within GFF142 at 137.893 kb on + strand, within GFF142 at 137.893 kb on + strand, within GFF142 at 137.931 kb on + strand, within GFF142 at 137.937 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.953 kb on + strand, within GFF142 at 137.961 kb on + strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.961 kb on - strand at 137.969 kb on - strand at 137.969 kb on - strand at 137.969 kb on - strand at 137.991 kb on - strand at 137.994 kb on + strand at 138.000 kb on + strand at 138.007 kb on - strand at 138.008 kb on - strand at 138.013 kb on + strand at 138.021 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 135,696 - +0.4 135,905 + -0.6 135,909 + +1.1 135,917 - +0.1 135,970 - +0.1 136,018 + GFF141 0.14 -0.0 136,026 - GFF141 0.14 -1.6 136,026 - GFF141 0.14 +0.2 136,026 - GFF141 0.14 +1.0 136,163 - GFF141 0.26 -0.2 136,236 + GFF141 0.32 -0.4 136,323 - GFF141 0.40 -0.9 136,402 + GFF141 0.46 -1.1 136,440 - GFF141 0.50 +0.6 136,454 + GFF141 0.51 -0.8 136,471 + GFF141 0.52 -0.8 136,471 + GFF141 0.52 -1.0 136,479 - GFF141 0.53 +1.4 136,485 - GFF141 0.53 -1.6 136,555 + GFF141 0.59 -1.1 136,563 - GFF141 0.60 -0.8 136,572 + GFF141 0.61 -1.1 136,580 - GFF141 0.62 -1.5 136,618 + GFF141 0.65 -2.3 136,632 + GFF141 0.66 -0.8 136,633 + GFF141 0.66 +1.0 136,652 + GFF141 0.68 -1.0 136,666 - GFF141 0.69 +0.2 136,728 - GFF141 0.74 -0.4 136,757 + GFF141 0.77 -1.8 136,757 + GFF141 0.77 -1.0 136,760 - GFF141 0.77 +0.8 136,765 - GFF141 0.77 -1.0 136,765 - GFF141 0.77 -0.8 136,765 - GFF141 0.77 -0.8 136,775 + GFF141 0.78 -1.0 136,829 + GFF141 0.83 +0.6 136,839 + GFF141 0.84 +0.7 136,839 + GFF141 0.84 -0.6 136,839 + GFF141 0.84 -1.6 136,839 + GFF141 0.84 -1.0 136,839 + GFF141 0.84 +0.5 136,847 - GFF141 0.84 -1.2 136,847 - GFF141 0.84 -1.5 136,847 - GFF141 0.84 -1.3 136,847 - GFF141 0.84 -0.7 136,858 - GFF141 0.85 -1.8 136,863 + GFF141 0.86 +0.8 136,917 - -0.8 137,048 - -2.1 137,076 - +0.5 137,101 + +1.1 137,101 + +0.1 137,101 + -1.0 137,101 + -4.3 137,109 - -0.8 137,109 - -2.0 137,109 - -1.3 137,109 - -2.3 137,109 - -1.6 137,109 - -1.0 137,121 - -3.6 137,121 - -2.7 137,127 + -2.9 137,144 + -1.9 137,157 - -1.8 137,164 + -1.7 137,164 + -2.0 137,171 + -1.8 137,172 - -2.5 137,200 + GFF142 0.12 -2.1 137,203 - GFF142 0.13 -3.0 137,213 - GFF142 0.14 -2.2 137,253 + GFF142 0.18 -2.4 137,256 - GFF142 0.18 -1.6 137,256 - GFF142 0.18 -2.4 137,261 - GFF142 0.18 -2.4 137,261 - GFF142 0.18 -2.0 137,273 + GFF142 0.20 -3.0 137,281 - GFF142 0.21 -2.8 137,363 + GFF142 0.29 -2.4 137,366 - GFF142 0.29 -4.3 137,371 - GFF142 0.30 -3.7 137,371 - GFF142 0.30 -0.2 137,380 + GFF142 0.31 -1.8 137,380 + GFF142 0.31 -3.8 137,383 - GFF142 0.31 -4.0 137,388 - GFF142 0.31 -1.8 137,390 + GFF142 0.32 -1.1 137,390 + GFF142 0.32 -2.6 137,390 + GFF142 0.32 -2.0 137,390 + GFF142 0.32 -3.6 137,390 + GFF142 0.32 -1.2 137,390 + GFF142 0.32 -0.6 137,390 + GFF142 0.32 -4.3 137,390 + GFF142 0.32 -2.2 137,390 + GFF142 0.32 -3.5 137,390 + GFF142 0.32 -3.9 137,390 + GFF142 0.32 -1.8 137,390 + GFF142 0.32 -1.3 137,398 - GFF142 0.33 -4.1 137,398 - GFF142 0.33 -2.9 137,398 - GFF142 0.33 -2.7 137,398 - GFF142 0.33 -2.8 137,398 - GFF142 0.33 -1.3 137,398 - GFF142 0.33 -3.0 137,398 - GFF142 0.33 -1.5 137,398 - GFF142 0.33 -2.1 137,398 - GFF142 0.33 -2.5 137,398 - GFF142 0.33 -2.8 137,427 - GFF142 0.35 +1.3 137,427 - GFF142 0.35 -1.1 137,449 - GFF142 0.38 -0.5 137,464 - GFF142 0.39 -2.6 137,464 - GFF142 0.39 -3.0 137,475 + GFF142 0.40 -3.4 137,478 - GFF142 0.41 -1.0 137,492 - GFF142 0.42 -2.9 137,515 + GFF142 0.45 -3.2 137,520 + GFF142 0.45 -1.6 137,523 - GFF142 0.45 -3.3 137,531 + GFF142 0.46 +0.8 137,542 + GFF142 0.47 -2.8 137,550 - GFF142 0.48 -0.9 137,550 - GFF142 0.48 -3.1 137,555 + GFF142 0.49 -2.4 137,555 + GFF142 0.49 -3.3 137,555 + GFF142 0.49 -2.5 137,555 + GFF142 0.49 -3.5 137,555 + GFF142 0.49 -2.3 137,561 - GFF142 0.49 -0.2 137,563 - GFF142 0.49 -2.8 137,563 - GFF142 0.49 -1.9 137,563 - GFF142 0.49 -1.9 137,563 - GFF142 0.49 -1.5 137,568 - GFF142 0.50 -1.0 137,580 - GFF142 0.51 -2.3 137,580 - GFF142 0.51 -3.0 137,587 + GFF142 0.52 -1.5 137,603 + GFF142 0.54 -0.4 137,603 + GFF142 0.54 -2.4 137,611 - GFF142 0.54 -3.7 137,611 - GFF142 0.54 -3.0 137,611 - GFF142 0.54 -1.4 137,632 - GFF142 0.57 -2.5 137,638 - GFF142 0.57 -2.6 137,642 + GFF142 0.58 -1.1 137,642 + GFF142 0.58 -2.6 137,642 + GFF142 0.58 -2.4 137,642 + GFF142 0.58 -2.8 137,642 + GFF142 0.58 -2.5 137,645 - GFF142 0.58 -3.9 137,650 - GFF142 0.58 -3.4 137,650 - GFF142 0.58 -1.7 137,650 - GFF142 0.58 -3.4 137,650 - GFF142 0.58 -2.2 137,650 - GFF142 0.58 -3.4 137,650 - GFF142 0.58 -2.3 137,650 - GFF142 0.58 -0.2 137,678 - GFF142 0.61 -3.8 137,680 + GFF142 0.61 -1.6 137,680 + GFF142 0.61 -2.5 137,680 + GFF142 0.61 -0.8 137,680 + GFF142 0.61 -3.2 137,688 - GFF142 0.62 -0.8 137,688 - GFF142 0.62 -0.8 137,688 - GFF142 0.62 -3.8 137,688 - GFF142 0.62 -3.5 137,688 - GFF142 0.62 -1.8 137,688 - GFF142 0.62 -1.2 137,695 + GFF142 0.63 -3.5 137,703 - GFF142 0.64 -2.7 137,703 - GFF142 0.64 -0.9 137,708 + GFF142 0.64 -2.5 137,716 - GFF142 0.65 -2.9 137,727 + GFF142 0.66 -2.3 137,727 + GFF142 0.66 -3.4 137,727 + GFF142 0.66 -1.0 137,727 + GFF142 0.66 -2.0 137,728 + GFF142 0.66 -3.5 137,728 + GFF142 0.66 +0.1 137,735 - GFF142 0.67 -2.1 137,736 - GFF142 0.67 -2.6 137,746 - GFF142 0.68 -1.7 137,796 - GFF142 0.73 +0.2 137,803 + GFF142 0.74 -0.3 137,834 + GFF142 0.77 -2.2 137,839 + GFF142 0.78 -2.5 137,839 + GFF142 0.78 -2.4 137,839 + GFF142 0.78 -1.6 137,860 + GFF142 0.80 -4.1 137,861 + GFF142 0.80 -1.0 137,861 + GFF142 0.80 -2.3 137,861 + GFF142 0.80 -2.7 137,861 + GFF142 0.80 -2.7 137,868 - GFF142 0.81 -1.4 137,868 - GFF142 0.81 -2.6 137,869 + GFF142 0.81 -2.2 137,869 - GFF142 0.81 -2.2 137,869 - GFF142 0.81 -1.4 137,872 + GFF142 0.81 -1.6 137,872 + GFF142 0.81 -1.7 137,893 + GFF142 0.83 -1.1 137,893 + GFF142 0.83 -2.7 137,931 + GFF142 0.87 -3.0 137,937 + GFF142 0.88 -2.5 137,953 + GFF142 0.89 -3.2 137,953 + GFF142 0.89 +0.6 137,953 + GFF142 0.89 -1.7 137,953 + GFF142 0.89 -1.4 137,953 + GFF142 0.89 -3.6 137,953 + GFF142 0.89 -3.0 137,953 + GFF142 0.89 -4.0 137,961 + -0.6 137,961 - -0.7 137,961 - -3.5 137,961 - -1.5 137,961 - -1.0 137,961 - -1.6 137,961 - -3.4 137,961 - -3.3 137,969 - -2.5 137,969 - -1.0 137,969 - -3.0 137,991 - -3.0 137,994 + -2.2 138,000 + -2.7 138,007 - -1.3 138,008 - -3.0 138,013 + -1.6 138,021 - -2.4
Or see this region's nucleotide sequence