Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1267 and GFF1268 are separated by 321 nucleotides GFF1268 and GFF1269 overlap by 1 nucleotides GFF1269 and GFF1270 are separated by 188 nucleotides
GFF1267 - UDP-glucose 6-dehydrogenase (EC 1.1.1.22), at 1,296,513 to 1,297,856
GFF1267
GFF1268 - hypothetical protein, at 1,298,178 to 1,298,525
GFF1268
GFF1269 - Serine acetyltransferase (EC 2.3.1.30), at 1,298,525 to 1,299,241
GFF1269
GFF1270 - hypothetical protein, at 1,299,430 to 1,300,203
GFF1270
Position (kb)
1298
1299
1300 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1297.527 kb on - strand, within GFF1267 at 1297.557 kb on - strand, within GFF1267 at 1297.563 kb on + strand, within GFF1267 at 1297.665 kb on + strand, within GFF1267 at 1297.670 kb on - strand, within GFF1267 at 1297.676 kb on + strand, within GFF1267 at 1297.676 kb on + strand, within GFF1267 at 1297.683 kb on - strand, within GFF1267 at 1297.684 kb on - strand, within GFF1267 at 1297.714 kb on - strand, within GFF1267 at 1297.720 kb on - strand, within GFF1267 at 1297.760 kb on + strand at 1297.760 kb on - strand at 1297.824 kb on + strand at 1297.824 kb on + strand at 1297.828 kb on - strand at 1297.877 kb on + strand at 1297.885 kb on - strand at 1297.906 kb on - strand at 1297.925 kb on - strand at 1297.925 kb on - strand at 1297.925 kb on - strand at 1297.935 kb on - strand at 1297.935 kb on - strand at 1297.966 kb on + strand at 1297.966 kb on + strand at 1297.974 kb on - strand at 1297.974 kb on - strand at 1297.974 kb on - strand at 1297.983 kb on + strand at 1298.010 kb on + strand at 1298.010 kb on + strand at 1298.021 kb on + strand at 1298.052 kb on + strand at 1298.060 kb on - strand at 1298.060 kb on - strand at 1298.062 kb on + strand at 1298.074 kb on + strand at 1298.093 kb on - strand at 1298.104 kb on + strand at 1298.104 kb on + strand at 1298.113 kb on - strand at 1298.139 kb on - strand at 1298.184 kb on - strand at 1298.237 kb on - strand, within GFF1268 at 1298.253 kb on - strand, within GFF1268 at 1298.253 kb on - strand, within GFF1268 at 1298.274 kb on - strand, within GFF1268 at 1298.281 kb on + strand, within GFF1268 at 1298.335 kb on - strand, within GFF1268 at 1298.344 kb on - strand, within GFF1268 at 1298.383 kb on + strand, within GFF1268 at 1298.385 kb on - strand, within GFF1268 at 1298.408 kb on + strand, within GFF1268 at 1298.467 kb on + strand, within GFF1268 at 1298.467 kb on + strand, within GFF1268 at 1298.467 kb on + strand, within GFF1268 at 1298.467 kb on + strand, within GFF1268 at 1298.510 kb on + strand at 1298.531 kb on - strand at 1298.531 kb on - strand at 1298.531 kb on - strand at 1298.531 kb on - strand at 1298.531 kb on - strand at 1298.541 kb on + strand at 1298.542 kb on + strand at 1298.549 kb on - strand at 1298.550 kb on - strand at 1298.628 kb on + strand, within GFF1269 at 1298.682 kb on - strand, within GFF1269 at 1298.682 kb on - strand, within GFF1269 at 1298.713 kb on + strand, within GFF1269 at 1298.719 kb on - strand, within GFF1269 at 1298.769 kb on + strand, within GFF1269 at 1298.801 kb on + strand, within GFF1269 at 1298.925 kb on - strand, within GFF1269 at 1298.944 kb on + strand, within GFF1269 at 1298.950 kb on + strand, within GFF1269 at 1298.958 kb on - strand, within GFF1269 at 1299.101 kb on - strand, within GFF1269 at 1299.101 kb on - strand, within GFF1269 at 1299.147 kb on - strand, within GFF1269 at 1299.187 kb on + strand at 1299.187 kb on + strand at 1299.195 kb on - strand at 1299.236 kb on - strand at 1299.249 kb on + strand at 1299.262 kb on - strand at 1299.267 kb on - strand at 1299.289 kb on - strand at 1299.289 kb on - strand at 1299.299 kb on - strand at 1299.402 kb on + strand at 1299.402 kb on + strand at 1299.470 kb on + strand at 1299.471 kb on - strand at 1299.478 kb on - strand at 1299.478 kb on - strand at 1299.517 kb on + strand, within GFF1270 at 1299.530 kb on - strand, within GFF1270 at 1299.553 kb on - strand, within GFF1270 at 1299.555 kb on + strand, within GFF1270 at 1299.570 kb on + strand, within GFF1270 at 1299.601 kb on + strand, within GFF1270 at 1299.609 kb on - strand, within GFF1270 at 1299.614 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.635 kb on + strand, within GFF1270 at 1299.640 kb on + strand, within GFF1270 at 1299.640 kb on + strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.643 kb on - strand, within GFF1270 at 1299.648 kb on - strand, within GFF1270 at 1299.659 kb on + strand, within GFF1270 at 1299.696 kb on + strand, within GFF1270 at 1299.713 kb on + strand, within GFF1270 at 1299.717 kb on - strand, within GFF1270 at 1299.726 kb on + strand, within GFF1270 at 1299.734 kb on - strand, within GFF1270 at 1299.755 kb on - strand, within GFF1270 at 1299.768 kb on - strand, within GFF1270 at 1299.793 kb on - strand, within GFF1270 at 1299.793 kb on - strand, within GFF1270 at 1299.793 kb on - strand, within GFF1270 at 1299.810 kb on + strand, within GFF1270 at 1299.833 kb on + strand, within GFF1270 at 1299.858 kb on - strand, within GFF1270 at 1299.896 kb on + strand, within GFF1270 at 1299.902 kb on + strand, within GFF1270 at 1299.914 kb on + strand, within GFF1270 at 1299.914 kb on + strand, within GFF1270 at 1299.914 kb on + strand, within GFF1270 at 1299.917 kb on - strand, within GFF1270 at 1299.922 kb on - strand, within GFF1270 at 1299.922 kb on - strand, within GFF1270 at 1299.961 kb on + strand, within GFF1270 at 1299.961 kb on + strand, within GFF1270 at 1299.962 kb on - strand, within GFF1270 at 1299.969 kb on - strand, within GFF1270 at 1299.973 kb on + strand, within GFF1270 at 1299.977 kb on + strand, within GFF1270 at 1299.985 kb on - strand, within GFF1270 at 1299.989 kb on + strand, within GFF1270 at 1300.043 kb on + strand, within GFF1270 at 1300.062 kb on + strand, within GFF1270 at 1300.070 kb on - strand, within GFF1270 at 1300.114 kb on - strand, within GFF1270 at 1300.118 kb on - strand, within GFF1270 at 1300.136 kb on - strand at 1300.150 kb on - strand at 1300.154 kb on - strand at 1300.156 kb on - strand at 1300.165 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 1,297,527 - GFF1267 0.75 -0.2 1,297,557 - GFF1267 0.78 +1.2 1,297,563 + GFF1267 0.78 +1.6 1,297,665 + GFF1267 0.86 +0.9 1,297,670 - GFF1267 0.86 +0.8 1,297,676 + GFF1267 0.87 +0.0 1,297,676 + GFF1267 0.87 +0.6 1,297,683 - GFF1267 0.87 -0.4 1,297,684 - GFF1267 0.87 +0.3 1,297,714 - GFF1267 0.89 -0.8 1,297,720 - GFF1267 0.90 +0.1 1,297,760 + +0.2 1,297,760 - +0.7 1,297,824 + -0.9 1,297,824 + -0.6 1,297,828 - -0.5 1,297,877 + -0.1 1,297,885 - -1.1 1,297,906 - -2.9 1,297,925 - -1.1 1,297,925 - -0.2 1,297,925 - -0.4 1,297,935 - -0.9 1,297,935 - +0.8 1,297,966 + +0.6 1,297,966 + +0.8 1,297,974 - +0.8 1,297,974 - +0.1 1,297,974 - +1.3 1,297,983 + +0.7 1,298,010 + +1.3 1,298,010 + +0.3 1,298,021 + -0.5 1,298,052 + -2.3 1,298,060 - +0.8 1,298,060 - +0.0 1,298,062 + -0.1 1,298,074 + -1.2 1,298,093 - +0.1 1,298,104 + +0.1 1,298,104 + -0.2 1,298,113 - +0.6 1,298,139 - +1.7 1,298,184 - -1.8 1,298,237 - GFF1268 0.17 -0.4 1,298,253 - GFF1268 0.22 +0.8 1,298,253 - GFF1268 0.22 -1.0 1,298,274 - GFF1268 0.28 +0.2 1,298,281 + GFF1268 0.30 -0.5 1,298,335 - GFF1268 0.45 -0.0 1,298,344 - GFF1268 0.48 -0.1 1,298,383 + GFF1268 0.59 -1.0 1,298,385 - GFF1268 0.59 -1.4 1,298,408 + GFF1268 0.66 +2.1 1,298,467 + GFF1268 0.83 -0.7 1,298,467 + GFF1268 0.83 -0.1 1,298,467 + GFF1268 0.83 +0.5 1,298,467 + GFF1268 0.83 -0.2 1,298,510 + +0.7 1,298,531 - -0.2 1,298,531 - -0.2 1,298,531 - +0.1 1,298,531 - +0.3 1,298,531 - +0.6 1,298,541 + -1.3 1,298,542 + +0.4 1,298,549 - -1.5 1,298,550 - +0.9 1,298,628 + GFF1269 0.14 -0.8 1,298,682 - GFF1269 0.22 -0.4 1,298,682 - GFF1269 0.22 +0.9 1,298,713 + GFF1269 0.26 +1.1 1,298,719 - GFF1269 0.27 -0.5 1,298,769 + GFF1269 0.34 +0.6 1,298,801 + GFF1269 0.38 +0.1 1,298,925 - GFF1269 0.56 +1.0 1,298,944 + GFF1269 0.58 -0.2 1,298,950 + GFF1269 0.59 -1.3 1,298,958 - GFF1269 0.60 -2.0 1,299,101 - GFF1269 0.80 -0.2 1,299,101 - GFF1269 0.80 -1.4 1,299,147 - GFF1269 0.87 -0.0 1,299,187 + -0.5 1,299,187 + +0.0 1,299,195 - -1.2 1,299,236 - +0.4 1,299,249 + -0.3 1,299,262 - -0.1 1,299,267 - +1.2 1,299,289 - -1.4 1,299,289 - -0.6 1,299,299 - +1.1 1,299,402 + -0.6 1,299,402 + +0.2 1,299,470 + -0.7 1,299,471 - -1.3 1,299,478 - -0.3 1,299,478 - -1.0 1,299,517 + GFF1270 0.11 +1.0 1,299,530 - GFF1270 0.13 +1.4 1,299,553 - GFF1270 0.16 +0.2 1,299,555 + GFF1270 0.16 +0.0 1,299,570 + GFF1270 0.18 -1.8 1,299,601 + GFF1270 0.22 +0.3 1,299,609 - GFF1270 0.23 -0.2 1,299,614 + GFF1270 0.24 +0.5 1,299,635 + GFF1270 0.26 +0.2 1,299,635 + GFF1270 0.26 +1.1 1,299,635 + GFF1270 0.26 +0.7 1,299,635 + GFF1270 0.26 +0.7 1,299,635 + GFF1270 0.26 +0.4 1,299,635 + GFF1270 0.26 -0.1 1,299,635 + GFF1270 0.26 +0.4 1,299,635 + GFF1270 0.26 +0.5 1,299,635 + GFF1270 0.26 -0.1 1,299,635 + GFF1270 0.26 +1.2 1,299,635 + GFF1270 0.26 -0.5 1,299,640 + GFF1270 0.27 +0.5 1,299,640 + GFF1270 0.27 -0.5 1,299,643 - GFF1270 0.28 -1.1 1,299,643 - GFF1270 0.28 -0.8 1,299,643 - GFF1270 0.28 -0.9 1,299,643 - GFF1270 0.28 -0.6 1,299,643 - GFF1270 0.28 -0.5 1,299,643 - GFF1270 0.28 -0.0 1,299,643 - GFF1270 0.28 -0.6 1,299,643 - GFF1270 0.28 -0.4 1,299,643 - GFF1270 0.28 -0.8 1,299,643 - GFF1270 0.28 -0.5 1,299,643 - GFF1270 0.28 -1.1 1,299,643 - GFF1270 0.28 -1.0 1,299,643 - GFF1270 0.28 -1.0 1,299,643 - GFF1270 0.28 -0.9 1,299,648 - GFF1270 0.28 -0.7 1,299,659 + GFF1270 0.30 -0.0 1,299,696 + GFF1270 0.34 -1.0 1,299,713 + GFF1270 0.37 -0.0 1,299,717 - GFF1270 0.37 -1.4 1,299,726 + GFF1270 0.38 -0.1 1,299,734 - GFF1270 0.39 -0.1 1,299,755 - GFF1270 0.42 -1.7 1,299,768 - GFF1270 0.44 -1.9 1,299,793 - GFF1270 0.47 -1.0 1,299,793 - GFF1270 0.47 +0.3 1,299,793 - GFF1270 0.47 +0.3 1,299,810 + GFF1270 0.49 +0.1 1,299,833 + GFF1270 0.52 -0.6 1,299,858 - GFF1270 0.55 +0.1 1,299,896 + GFF1270 0.60 -3.2 1,299,902 + GFF1270 0.61 -0.8 1,299,914 + GFF1270 0.63 +0.1 1,299,914 + GFF1270 0.63 +0.4 1,299,914 + GFF1270 0.63 -0.2 1,299,917 - GFF1270 0.63 +0.8 1,299,922 - GFF1270 0.64 -0.1 1,299,922 - GFF1270 0.64 -0.4 1,299,961 + GFF1270 0.69 -0.2 1,299,961 + GFF1270 0.69 +1.9 1,299,962 - GFF1270 0.69 -0.1 1,299,969 - GFF1270 0.70 +1.6 1,299,973 + GFF1270 0.70 -0.4 1,299,977 + GFF1270 0.71 -0.0 1,299,985 - GFF1270 0.72 +0.3 1,299,989 + GFF1270 0.72 -0.5 1,300,043 + GFF1270 0.79 +0.2 1,300,062 + GFF1270 0.82 +1.3 1,300,070 - GFF1270 0.83 -0.8 1,300,114 - GFF1270 0.88 -1.2 1,300,118 - GFF1270 0.89 -1.2 1,300,136 - +0.3 1,300,150 - +0.8 1,300,154 - -0.4 1,300,156 - -0.4 1,300,165 + -0.3
Or see this region's nucleotide sequence