Strain Fitness in Sphingobium sp. HT1-2 around GFF10

Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7 and GFF8 are separated by 281 nucleotidesGFF8 and GFF9 are separated by 88 nucleotidesGFF9 and GFF10 are separated by 93 nucleotidesGFF10 and GFF11 are separated by 156 nucleotides GFF7 - hypothetical protein, at 4,389 to 4,778 GFF7 GFF8 - hypothetical protein, at 5,060 to 5,461 GFF8 GFF9 - hypothetical protein, at 5,550 to 5,663 GFF9 GFF10 - hypothetical protein, at 5,757 to 6,065 GFF10 GFF11 - hypothetical protein, at 6,222 to 7,784 GFF11 Position (kb) 5 6 7Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4.757 kb on - strandat 4.801 kb on - strandat 4.858 kb on - strandat 4.888 kb on - strandat 5.080 kb on + strandat 5.151 kb on + strand, within GFF8at 5.151 kb on + strand, within GFF8at 5.151 kb on + strand, within GFF8at 5.154 kb on - strand, within GFF8at 5.176 kb on + strand, within GFF8at 5.176 kb on + strand, within GFF8at 5.210 kb on - strand, within GFF8at 5.212 kb on + strand, within GFF8at 5.281 kb on - strand, within GFF8at 5.295 kb on + strand, within GFF8at 5.308 kb on + strand, within GFF8at 5.308 kb on + strand, within GFF8at 5.324 kb on - strand, within GFF8at 5.343 kb on + strand, within GFF8at 5.392 kb on + strand, within GFF8at 5.392 kb on + strand, within GFF8at 5.400 kb on - strand, within GFF8at 5.407 kb on + strand, within GFF8at 5.431 kb on + strandat 5.447 kb on - strandat 5.464 kb on - strandat 5.518 kb on - strandat 5.626 kb on + strand, within GFF9at 5.636 kb on + strand, within GFF9at 5.636 kb on + strand, within GFF9at 5.663 kb on - strandat 5.686 kb on - strandat 5.696 kb on + strandat 5.696 kb on + strandat 5.702 kb on - strandat 5.704 kb on - strandat 5.746 kb on + strandat 5.746 kb on + strandat 5.747 kb on + strandat 5.760 kb on + strandat 5.777 kb on - strandat 5.782 kb on - strandat 5.914 kb on - strand, within GFF10at 6.183 kb on + strandat 6.245 kb on - strandat 6.273 kb on + strandat 6.281 kb on - strandat 6.281 kb on - strandat 6.281 kb on - strandat 6.302 kb on + strandat 6.322 kb on - strandat 6.341 kb on + strandat 6.349 kb on - strandat 6.351 kb on + strandat 6.354 kb on - strandat 6.356 kb on + strandat 6.356 kb on + strandat 6.357 kb on + strandat 6.357 kb on + strandat 6.357 kb on + strandat 6.359 kb on - strandat 6.364 kb on - strandat 6.365 kb on - strandat 6.403 kb on - strand, within GFF11at 6.411 kb on - strand, within GFF11at 6.497 kb on - strand, within GFF11at 6.519 kb on + strand, within GFF11at 6.519 kb on + strand, within GFF11at 6.519 kb on + strand, within GFF11at 6.519 kb on + strand, within GFF11at 6.519 kb on + strand, within GFF11at 6.521 kb on + strand, within GFF11at 6.527 kb on - strand, within GFF11at 6.540 kb on - strand, within GFF11at 6.558 kb on + strand, within GFF11at 6.566 kb on - strand, within GFF11at 6.566 kb on - strand, within GFF11at 6.566 kb on - strand, within GFF11at 6.566 kb on - strand, within GFF11at 6.582 kb on + strand, within GFF11at 6.590 kb on - strand, within GFF11at 6.590 kb on - strand, within GFF11at 6.618 kb on + strand, within GFF11at 6.624 kb on - strand, within GFF11at 6.665 kb on - strand, within GFF11at 6.686 kb on + strand, within GFF11at 6.692 kb on + strand, within GFF11at 6.727 kb on + strand, within GFF11at 6.740 kb on - strand, within GFF11at 6.740 kb on - strand, within GFF11at 6.787 kb on - strand, within GFF11at 6.798 kb on + strand, within GFF11at 6.868 kb on + strand, within GFF11at 6.873 kb on + strand, within GFF11at 6.881 kb on - strand, within GFF11at 6.885 kb on + strand, within GFF11at 6.905 kb on + strand, within GFF11at 6.913 kb on - strand, within GFF11at 6.914 kb on + strand, within GFF11at 6.923 kb on + strand, within GFF11at 6.931 kb on - strand, within GFF11at 6.962 kb on + strand, within GFF11at 6.965 kb on - strand, within GFF11at 6.985 kb on + strand, within GFF11at 6.993 kb on - strand, within GFF11at 6.995 kb on - strand, within GFF11at 7.036 kb on + strand, within GFF11at 7.037 kb on - strand, within GFF11at 7.040 kb on - strand, within GFF11at 7.043 kb on + strand, within GFF11at 7.045 kb on - strand, within GFF11at 7.063 kb on + strand, within GFF11

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate
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4,757 - -2.2
4,801 - +1.2
4,858 - -0.7
4,888 - -0.4
5,080 + +0.2
5,151 + GFF8 0.23 +0.7
5,151 + GFF8 0.23 -0.2
5,151 + GFF8 0.23 +0.2
5,154 - GFF8 0.23 +0.7
5,176 + GFF8 0.29 -0.2
5,176 + GFF8 0.29 -2.0
5,210 - GFF8 0.37 -1.4
5,212 + GFF8 0.38 +0.1
5,281 - GFF8 0.55 +0.4
5,295 + GFF8 0.58 -0.8
5,308 + GFF8 0.62 -2.0
5,308 + GFF8 0.62 +0.7
5,324 - GFF8 0.66 +1.4
5,343 + GFF8 0.70 +0.5
5,392 + GFF8 0.83 +0.3
5,392 + GFF8 0.83 +0.5
5,400 - GFF8 0.85 -0.4
5,407 + GFF8 0.86 +1.1
5,431 + -1.1
5,447 - -0.2
5,464 - +0.4
5,518 - +0.7
5,626 + GFF9 0.67 +0.4
5,636 + GFF9 0.75 +0.7
5,636 + GFF9 0.75 -0.1
5,663 - +1.0
5,686 - -0.2
5,696 + -0.9
5,696 + +0.2
5,702 - +1.3
5,704 - -1.2
5,746 + +0.3
5,746 + +0.0
5,747 + -2.0
5,760 + +0.5
5,777 - -1.4
5,782 - +0.6
5,914 - GFF10 0.51 -1.6
6,183 + -0.8
6,245 - -0.8
6,273 + +1.1
6,281 - -1.6
6,281 - -1.0
6,281 - -2.4
6,302 + +0.8
6,322 - -0.8
6,341 + +1.1
6,349 - +0.9
6,351 + +1.4
6,354 - -1.0
6,356 + -0.7
6,356 + +0.9
6,357 + -0.1
6,357 + -1.9
6,357 + +1.1
6,359 - +0.2
6,364 - -0.9
6,365 - -0.8
6,403 - GFF11 0.12 +0.3
6,411 - GFF11 0.12 -0.2
6,497 - GFF11 0.18 +1.3
6,519 + GFF11 0.19 +0.3
6,519 + GFF11 0.19 +0.2
6,519 + GFF11 0.19 -0.3
6,519 + GFF11 0.19 -0.5
6,519 + GFF11 0.19 +1.9
6,521 + GFF11 0.19 +0.0
6,527 - GFF11 0.20 +1.3
6,540 - GFF11 0.20 +0.6
6,558 + GFF11 0.21 +0.6
6,566 - GFF11 0.22 -0.1
6,566 - GFF11 0.22 +0.4
6,566 - GFF11 0.22 +0.6
6,566 - GFF11 0.22 -0.6
6,582 + GFF11 0.23 -0.2
6,590 - GFF11 0.24 +0.1
6,590 - GFF11 0.24 +0.2
6,618 + GFF11 0.25 -0.3
6,624 - GFF11 0.26 -0.5
6,665 - GFF11 0.28 +0.1
6,686 + GFF11 0.30 -1.0
6,692 + GFF11 0.30 +1.1
6,727 + GFF11 0.32 -0.9
6,740 - GFF11 0.33 -2.5
6,740 - GFF11 0.33 +0.0
6,787 - GFF11 0.36 -1.5
6,798 + GFF11 0.37 -0.3
6,868 + GFF11 0.41 -0.2
6,873 + GFF11 0.42 -2.9
6,881 - GFF11 0.42 -0.2
6,885 + GFF11 0.42 +0.2
6,905 + GFF11 0.44 -0.3
6,913 - GFF11 0.44 +0.6
6,914 + GFF11 0.44 -0.8
6,923 + GFF11 0.45 +0.5
6,931 - GFF11 0.45 +0.8
6,962 + GFF11 0.47 -0.8
6,965 - GFF11 0.48 +1.4
6,985 + GFF11 0.49 +1.8
6,993 - GFF11 0.49 -0.1
6,995 - GFF11 0.49 -1.0
7,036 + GFF11 0.52 -1.4
7,037 - GFF11 0.52 +0.2
7,040 - GFF11 0.52 +0.3
7,043 + GFF11 0.53 -0.5
7,045 - GFF11 0.53 +0.5
7,063 + GFF11 0.54 +0.8

Or see this region's nucleotide sequence