Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF80 and GFF81 are separated by 2 nucleotides GFF81 and GFF82 overlap by 4 nucleotides GFF82 and GFF83 overlap by 4 nucleotides
GFF80 - Cytochrome c-type biogenesis protein CcmC, putative heme lyase for CcmE, at 70,214 to 70,936
GFF80
GFF81 - hypothetical protein, at 70,939 to 71,070
GFF81
GFF82 - Cytochrome c-type biogenesis protein CcmE, heme chaperone, at 71,067 to 71,513
GFF82
GFF83 - Cytochrome c heme lyase subunit CcmF, at 71,510 to 73,438
GFF83
Position (kb)
71
72 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 70.070 kb on + strand at 70.070 kb on + strand at 70.070 kb on + strand at 70.070 kb on + strand at 70.070 kb on + strand at 70.071 kb on + strand at 70.076 kb on - strand at 70.078 kb on - strand at 70.078 kb on - strand at 70.078 kb on - strand at 70.120 kb on + strand at 70.129 kb on + strand at 70.129 kb on + strand at 70.129 kb on + strand at 70.205 kb on + strand at 70.205 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.931 kb on + strand at 70.936 kb on - strand at 70.939 kb on - strand at 70.939 kb on - strand at 70.939 kb on - strand at 70.941 kb on - strand at 71.045 kb on - strand, within GFF81 at 71.051 kb on - strand, within GFF81 at 71.066 kb on + strand at 71.072 kb on + strand at 71.495 kb on + strand at 71.495 kb on + strand at 71.503 kb on - strand at 71.503 kb on - strand at 71.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 70,070 + +0.7 70,070 + -1.5 70,070 + -1.6 70,070 + -2.6 70,070 + -0.8 70,071 + -2.9 70,076 - -2.4 70,078 - +0.5 70,078 - -0.4 70,078 - -0.7 70,120 + +0.1 70,129 + -0.1 70,129 + +0.1 70,129 + -0.7 70,205 + +0.2 70,205 + -2.1 70,931 + +0.3 70,931 + +0.3 70,931 + +0.1 70,931 + -0.5 70,931 + +0.5 70,936 - -0.1 70,939 - -2.7 70,939 - -0.5 70,939 - -1.0 70,941 - +0.1 71,045 - GFF81 0.80 -2.5 71,051 - GFF81 0.85 -1.2 71,066 + +0.0 71,072 + -1.1 71,495 + -0.8 71,495 + -0.4 71,503 - +0.1 71,503 - -0.7 71,503 - -0.3
Or see this region's nucleotide sequence