Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF50 and GFF51 are separated by 50 nucleotides GFF51 and GFF52 are separated by 28 nucleotides GFF52 and GFF53 are separated by 18 nucleotides GFF53 and GFF54 are separated by 6 nucleotides
GFF50 - Phosphoglucomutase (EC 5.4.2.2), at 40,124 to 41,752
GFF50
GFF51 - hypothetical protein, at 41,803 to 41,949
GFF51
GFF52 - Choloylglycine hydrolase (EC 3.5.1.24), at 41,978 to 43,033
GFF52
GFF53 - FIG00450481: hypothetical protein, at 43,052 to 43,858
GFF53
GFF54 - Peptidyl-tRNA hydrolase (EC 3.1.1.29), at 43,865 to 44,434
GFF54
Position (kb) 41
42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 41.207 kb on - strand, within GFF50 at 41.418 kb on - strand, within GFF50 at 41.608 kb on - strand at 41.742 kb on + strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.750 kb on - strand at 41.752 kb on + strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.760 kb on - strand at 41.765 kb on - strand at 41.772 kb on - strand at 41.795 kb on - strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.803 kb on + strand at 41.808 kb on + strand at 41.813 kb on + strand at 41.816 kb on - strand at 41.816 kb on - strand at 41.883 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.894 kb on - strand, within GFF51 at 41.904 kb on + strand, within GFF51 at 41.944 kb on + strand at 41.950 kb on + strand at 41.950 kb on + strand at 41.958 kb on - strand at 41.981 kb on - strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.006 kb on + strand at 42.024 kb on - strand at 42.031 kb on - strand at 42.102 kb on + strand, within GFF52 at 42.136 kb on + strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.144 kb on - strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.156 kb on + strand, within GFF52 at 42.176 kb on - strand, within GFF52 at 42.180 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.264 kb on + strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.272 kb on - strand, within GFF52 at 42.335 kb on + strand, within GFF52 at 42.396 kb on - strand, within GFF52 at 42.475 kb on - strand, within GFF52 at 42.483 kb on + strand, within GFF52 at 42.494 kb on + strand, within GFF52 at 42.558 kb on + strand, within GFF52 at 42.609 kb on - strand, within GFF52 at 42.655 kb on + strand, within GFF52 at 42.662 kb on + strand, within GFF52 at 42.692 kb on + strand, within GFF52 at 42.692 kb on - strand, within GFF52 at 42.709 kb on + strand, within GFF52 at 42.711 kb on + strand, within GFF52 at 42.716 kb on + strand, within GFF52 at 42.724 kb on - strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.728 kb on + strand, within GFF52 at 42.736 kb on - strand, within GFF52 at 42.737 kb on + strand, within GFF52 at 42.741 kb on - strand, within GFF52 at 42.767 kb on + strand, within GFF52 at 42.796 kb on + strand, within GFF52 at 42.804 kb on - strand, within GFF52 at 42.804 kb on - strand, within GFF52 at 42.835 kb on + strand, within GFF52 at 42.835 kb on + strand, within GFF52 at 42.883 kb on + strand, within GFF52 at 42.894 kb on + strand, within GFF52 at 42.894 kb on + strand, within GFF52 at 42.895 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.909 kb on + strand, within GFF52 at 42.917 kb on - strand, within GFF52 at 42.917 kb on - strand, within GFF52 at 42.977 kb on - strand at 42.991 kb on + strand at 42.991 kb on + strand at 42.991 kb on + strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 42.999 kb on - strand at 43.027 kb on + strand at 43.027 kb on + strand at 43.027 kb on + strand at 43.027 kb on + strand at 43.027 kb on + strand at 43.035 kb on - strand at 43.035 kb on - strand at 43.035 kb on - strand at 43.035 kb on - strand at 43.045 kb on - strand at 43.064 kb on + strand at 43.072 kb on - strand at 43.084 kb on - strand at 43.103 kb on + strand at 43.162 kb on - strand, within GFF53 at 43.167 kb on - strand, within GFF53 at 43.181 kb on + strand, within GFF53 at 43.181 kb on + strand, within GFF53 at 43.189 kb on - strand, within GFF53 at 43.296 kb on + strand, within GFF53 at 43.309 kb on - strand, within GFF53 at 43.326 kb on - strand, within GFF53 at 43.327 kb on - strand, within GFF53 at 43.354 kb on - strand, within GFF53 at 43.364 kb on - strand, within GFF53 at 43.407 kb on + strand, within GFF53 at 43.407 kb on - strand, within GFF53 at 43.415 kb on - strand, within GFF53 at 43.429 kb on + strand, within GFF53 at 43.436 kb on + strand, within GFF53 at 43.449 kb on + strand, within GFF53 at 43.465 kb on + strand, within GFF53 at 43.492 kb on - strand, within GFF53 at 43.516 kb on - strand, within GFF53 at 43.516 kb on - strand, within GFF53 at 43.518 kb on + strand, within GFF53 at 43.544 kb on - strand, within GFF53 at 43.544 kb on - strand, within GFF53 at 43.549 kb on + strand, within GFF53 at 43.549 kb on + strand, within GFF53 at 43.549 kb on + strand, within GFF53 at 43.551 kb on + strand, within GFF53 at 43.553 kb on + strand, within GFF53 at 43.557 kb on - strand, within GFF53 at 43.559 kb on - strand, within GFF53 at 43.586 kb on - strand, within GFF53 at 43.642 kb on - strand, within GFF53 at 43.659 kb on + strand, within GFF53 at 43.659 kb on + strand, within GFF53 at 43.689 kb on + strand, within GFF53 at 43.704 kb on + strand, within GFF53 at 43.704 kb on + strand, within GFF53 at 43.704 kb on + strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.712 kb on - strand, within GFF53 at 43.724 kb on + strand, within GFF53 at 43.748 kb on + strand, within GFF53 at 43.748 kb on + strand, within GFF53 at 43.748 kb on + strand, within GFF53 at 43.748 kb on + strand, within GFF53 at 43.756 kb on - strand, within GFF53 at 43.756 kb on - strand, within GFF53 at 43.756 kb on - strand, within GFF53 at 43.758 kb on + strand, within GFF53 at 43.852 kb on + strand at 43.852 kb on + strand at 43.852 kb on + strand at 43.852 kb on + strand at 43.852 kb on + strand at 43.860 kb on - strand at 43.860 kb on - strand at 43.860 kb on - strand at 43.860 kb on - strand at 43.872 kb on + strand at 43.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 41,207 - GFF50 0.66 +1.2 41,418 - GFF50 0.79 +2.8 41,608 - +0.2 41,742 + +0.2 41,750 - +1.5 41,750 - +0.1 41,750 - -0.0 41,750 - -1.7 41,752 + -2.0 41,760 - +0.2 41,760 - +0.0 41,760 - -0.6 41,760 - -1.2 41,760 - -1.8 41,760 - -0.2 41,765 - -1.0 41,772 - -0.4 41,795 - -0.9 41,803 + -1.0 41,803 + -0.8 41,803 + +0.1 41,808 + +0.2 41,813 + -0.8 41,816 - +1.2 41,816 - -0.7 41,883 - GFF51 0.54 +0.5 41,894 - GFF51 0.62 -1.1 41,894 - GFF51 0.62 +0.8 41,904 + GFF51 0.69 -1.0 41,944 + -0.0 41,950 + -0.2 41,950 + -1.2 41,958 - -0.1 41,981 - +0.6 42,006 + -0.3 42,006 + +0.3 42,006 + -1.0 42,024 - -1.3 42,031 - +0.7 42,102 + GFF52 0.12 +0.2 42,136 + GFF52 0.15 +0.5 42,144 - GFF52 0.16 +0.6 42,144 - GFF52 0.16 -0.8 42,144 - GFF52 0.16 +0.5 42,156 + GFF52 0.17 +0.5 42,156 + GFF52 0.17 -0.1 42,176 - GFF52 0.19 -1.1 42,180 + GFF52 0.19 -0.6 42,264 + GFF52 0.27 +1.8 42,264 + GFF52 0.27 +1.1 42,264 + GFF52 0.27 -1.4 42,264 + GFF52 0.27 -0.0 42,264 + GFF52 0.27 +0.9 42,272 - GFF52 0.28 -0.5 42,272 - GFF52 0.28 +0.6 42,272 - GFF52 0.28 -0.7 42,335 + GFF52 0.34 +0.0 42,396 - GFF52 0.40 +0.1 42,475 - GFF52 0.47 +0.1 42,483 + GFF52 0.48 +0.1 42,494 + GFF52 0.49 -0.4 42,558 + GFF52 0.55 -2.4 42,609 - GFF52 0.60 +0.5 42,655 + GFF52 0.64 -1.4 42,662 + GFF52 0.65 -0.8 42,692 + GFF52 0.68 +0.6 42,692 - GFF52 0.68 -0.8 42,709 + GFF52 0.69 -1.6 42,711 + GFF52 0.69 +1.9 42,716 + GFF52 0.70 -0.1 42,724 - GFF52 0.71 -0.7 42,728 + GFF52 0.71 +0.1 42,728 + GFF52 0.71 +0.8 42,728 + GFF52 0.71 -0.2 42,728 + GFF52 0.71 -0.4 42,736 - GFF52 0.72 +0.7 42,737 + GFF52 0.72 -0.3 42,741 - GFF52 0.72 -0.9 42,767 + GFF52 0.75 -1.2 42,796 + GFF52 0.77 +0.3 42,804 - GFF52 0.78 +0.8 42,804 - GFF52 0.78 -0.2 42,835 + GFF52 0.81 +0.1 42,835 + GFF52 0.81 -0.2 42,883 + GFF52 0.86 -0.0 42,894 + GFF52 0.87 +0.7 42,894 + GFF52 0.87 +0.2 42,895 + GFF52 0.87 +0.3 42,909 + GFF52 0.88 +0.6 42,909 + GFF52 0.88 -0.3 42,909 + GFF52 0.88 +0.6 42,917 - GFF52 0.89 +0.8 42,917 - GFF52 0.89 +0.3 42,977 - +1.3 42,991 + -0.2 42,991 + -1.1 42,991 + +0.1 42,999 - -0.5 42,999 - +0.0 42,999 - +0.1 42,999 - +0.1 42,999 - -0.7 42,999 - +1.2 42,999 - -1.6 42,999 - +0.5 42,999 - +1.3 42,999 - +1.2 42,999 - -0.8 43,027 + -0.3 43,027 + +0.6 43,027 + -0.7 43,027 + +0.7 43,027 + +1.4 43,035 - -1.1 43,035 - -0.8 43,035 - +1.9 43,035 - -0.0 43,045 - +1.0 43,064 + -0.0 43,072 - +0.6 43,084 - +0.4 43,103 + +0.4 43,162 - GFF53 0.14 +0.5 43,167 - GFF53 0.14 +0.9 43,181 + GFF53 0.16 -1.0 43,181 + GFF53 0.16 -3.3 43,189 - GFF53 0.17 -1.2 43,296 + GFF53 0.30 +0.6 43,309 - GFF53 0.32 -0.4 43,326 - GFF53 0.34 -0.4 43,327 - GFF53 0.34 -0.0 43,354 - GFF53 0.37 +0.8 43,364 - GFF53 0.39 +0.8 43,407 + GFF53 0.44 -0.4 43,407 - GFF53 0.44 +2.0 43,415 - GFF53 0.45 +0.2 43,429 + GFF53 0.47 +0.4 43,436 + GFF53 0.48 -0.2 43,449 + GFF53 0.49 +0.1 43,465 + GFF53 0.51 -1.5 43,492 - GFF53 0.55 -0.2 43,516 - GFF53 0.57 -1.4 43,516 - GFF53 0.57 -1.1 43,518 + GFF53 0.58 +0.6 43,544 - GFF53 0.61 -0.4 43,544 - GFF53 0.61 +0.7 43,549 + GFF53 0.62 -0.3 43,549 + GFF53 0.62 +0.5 43,549 + GFF53 0.62 +0.6 43,551 + GFF53 0.62 +0.9 43,553 + GFF53 0.62 +1.9 43,557 - GFF53 0.63 -1.4 43,559 - GFF53 0.63 -1.6 43,586 - GFF53 0.66 -0.0 43,642 - GFF53 0.73 +0.3 43,659 + GFF53 0.75 -0.9 43,659 + GFF53 0.75 +0.5 43,689 + GFF53 0.79 +0.6 43,704 + GFF53 0.81 -0.3 43,704 + GFF53 0.81 -0.1 43,704 + GFF53 0.81 +0.0 43,712 - GFF53 0.82 +0.0 43,712 - GFF53 0.82 -2.1 43,712 - GFF53 0.82 -0.4 43,712 - GFF53 0.82 +0.3 43,712 - GFF53 0.82 +0.6 43,712 - GFF53 0.82 -0.7 43,724 + GFF53 0.83 +0.1 43,748 + GFF53 0.86 +1.2 43,748 + GFF53 0.86 +1.0 43,748 + GFF53 0.86 +1.2 43,748 + GFF53 0.86 -0.9 43,756 - GFF53 0.87 +0.5 43,756 - GFF53 0.87 -0.6 43,756 - GFF53 0.87 +0.2 43,758 + GFF53 0.87 -0.6 43,852 + -0.0 43,852 + -1.4 43,852 + +0.3 43,852 + -1.6 43,852 + -0.3 43,860 - -0.6 43,860 - -2.3 43,860 - -0.3 43,860 - +0.2 43,872 + +0.2 43,874 - +0.5
Or see this region's nucleotide sequence