Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF43 and GFF44 are separated by 100 nucleotides GFF44 and GFF45 are separated by 59 nucleotides GFF45 and GFF46 are separated by 45 nucleotides
GFF43 - Transporter, LysE family, at 33,443 to 34,069
GFF43
GFF44 - Acyl dehydratase, at 34,170 to 34,634
GFF44
GFF45 - secreted alkaline phosphatase, at 34,694 to 36,244
GFF45
GFF46 - Alkaline phosphodiesterase I (EC 3.1.4.1) / Nucleotide pyrophosphatase (EC 3.6.1.9), at 36,290 to 37,570
GFF46
Position (kb) 34
35
36
37 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 33.696 kb on + strand, within GFF43 at 33.699 kb on - strand, within GFF43 at 33.699 kb on - strand, within GFF43 at 33.708 kb on + strand, within GFF43 at 33.718 kb on + strand, within GFF43 at 33.718 kb on + strand, within GFF43 at 33.739 kb on - strand, within GFF43 at 33.769 kb on + strand, within GFF43 at 33.782 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.852 kb on - strand, within GFF43 at 33.855 kb on - strand, within GFF43 at 33.867 kb on + strand, within GFF43 at 33.867 kb on + strand, within GFF43 at 33.875 kb on - strand, within GFF43 at 33.930 kb on + strand, within GFF43 at 33.969 kb on - strand, within GFF43 at 33.990 kb on - strand, within GFF43 at 34.013 kb on + strand at 34.015 kb on + strand at 34.030 kb on + strand at 34.030 kb on + strand at 34.034 kb on - strand at 34.059 kb on + strand at 34.059 kb on + strand at 34.059 kb on + strand at 34.067 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.072 kb on - strand at 34.077 kb on - strand at 34.079 kb on - strand at 34.089 kb on - strand at 34.096 kb on - strand at 34.129 kb on + strand at 34.129 kb on + strand at 34.137 kb on - strand at 34.138 kb on + strand at 34.145 kb on - strand at 34.166 kb on - strand at 34.166 kb on - strand at 34.171 kb on - strand at 34.171 kb on - strand at 34.176 kb on - strand at 34.206 kb on + strand at 34.210 kb on - strand at 34.214 kb on + strand at 34.217 kb on - strand, within GFF44 at 34.217 kb on - strand, within GFF44 at 34.217 kb on - strand, within GFF44 at 34.227 kb on - strand, within GFF44 at 34.263 kb on + strand, within GFF44 at 34.271 kb on - strand, within GFF44 at 34.312 kb on + strand, within GFF44 at 34.323 kb on + strand, within GFF44 at 34.364 kb on - strand, within GFF44 at 34.366 kb on - strand, within GFF44 at 34.366 kb on - strand, within GFF44 at 34.377 kb on - strand, within GFF44 at 34.384 kb on - strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.442 kb on + strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.450 kb on - strand, within GFF44 at 34.455 kb on - strand, within GFF44 at 34.464 kb on + strand, within GFF44 at 34.464 kb on + strand, within GFF44 at 34.511 kb on + strand, within GFF44 at 34.511 kb on + strand, within GFF44 at 34.519 kb on - strand, within GFF44 at 34.525 kb on + strand, within GFF44 at 34.546 kb on - strand, within GFF44 at 34.670 kb on - strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.708 kb on + strand at 34.716 kb on - strand at 34.716 kb on - strand at 34.730 kb on + strand at 34.738 kb on - strand at 34.739 kb on - strand at 34.775 kb on - strand at 34.800 kb on + strand at 34.800 kb on + strand at 34.800 kb on + strand at 34.808 kb on - strand at 34.826 kb on + strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.834 kb on - strand at 34.846 kb on - strand at 34.879 kb on + strand, within GFF45 at 34.879 kb on - strand, within GFF45 at 34.903 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.009 kb on + strand, within GFF45 at 35.017 kb on - strand, within GFF45 at 35.034 kb on + strand, within GFF45 at 35.042 kb on - strand, within GFF45 at 35.060 kb on + strand, within GFF45 at 35.060 kb on + strand, within GFF45 at 35.068 kb on + strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.068 kb on - strand, within GFF45 at 35.076 kb on - strand, within GFF45 at 35.076 kb on - strand, within GFF45 at 35.077 kb on + strand, within GFF45 at 35.079 kb on + strand, within GFF45 at 35.142 kb on + strand, within GFF45 at 35.158 kb on + strand, within GFF45 at 35.166 kb on + strand, within GFF45 at 35.168 kb on + strand, within GFF45 at 35.193 kb on + strand, within GFF45 at 35.194 kb on + strand, within GFF45 at 35.218 kb on - strand, within GFF45 at 35.236 kb on + strand, within GFF45 at 35.244 kb on - strand, within GFF45 at 35.283 kb on + strand, within GFF45 at 35.288 kb on + strand, within GFF45 at 35.291 kb on - strand, within GFF45 at 35.296 kb on - strand, within GFF45 at 35.296 kb on - strand, within GFF45 at 35.359 kb on - strand, within GFF45 at 35.375 kb on - strand, within GFF45 at 35.392 kb on - strand, within GFF45 at 35.421 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.566 kb on + strand, within GFF45 at 35.581 kb on + strand, within GFF45 at 35.607 kb on - strand, within GFF45 at 35.614 kb on + strand, within GFF45 at 35.619 kb on + strand, within GFF45 at 35.619 kb on + strand, within GFF45 at 35.622 kb on - strand, within GFF45 at 35.647 kb on + strand, within GFF45 at 35.653 kb on - strand, within GFF45 at 35.653 kb on - strand, within GFF45 at 35.655 kb on - strand, within GFF45 at 35.700 kb on - strand, within GFF45 at 35.700 kb on - strand, within GFF45 at 35.732 kb on + strand, within GFF45 at 35.740 kb on - strand, within GFF45 at 35.766 kb on + strand, within GFF45 at 35.767 kb on - strand, within GFF45 at 35.774 kb on - strand, within GFF45 at 35.774 kb on - strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.821 kb on + strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.829 kb on - strand, within GFF45 at 35.848 kb on + strand, within GFF45 at 35.850 kb on + strand, within GFF45 at 35.850 kb on + strand, within GFF45 at 35.858 kb on - strand, within GFF45 at 35.863 kb on - strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.865 kb on + strand, within GFF45 at 35.875 kb on + strand, within GFF45 at 35.875 kb on + strand, within GFF45 at 35.883 kb on - strand, within GFF45 at 35.883 kb on - strand, within GFF45 at 35.887 kb on + strand, within GFF45 at 35.901 kb on + strand, within GFF45 at 35.901 kb on - strand, within GFF45 at 35.909 kb on - strand, within GFF45 at 35.994 kb on + strand, within GFF45 at 36.035 kb on + strand, within GFF45 at 36.085 kb on + strand, within GFF45 at 36.102 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.129 kb on + strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.137 kb on - strand at 36.146 kb on + strand at 36.154 kb on - strand at 36.224 kb on - strand at 36.253 kb on + strand at 36.261 kb on - strand at 36.288 kb on + strand at 36.294 kb on + strand at 36.294 kb on + strand at 36.308 kb on + strand at 36.308 kb on + strand at 36.316 kb on - strand at 36.324 kb on - strand at 36.356 kb on - strand at 36.419 kb on + strand, within GFF46 at 36.427 kb on - strand, within GFF46 at 36.449 kb on + strand, within GFF46 at 36.478 kb on + strand, within GFF46 at 36.487 kb on - strand, within GFF46 at 36.495 kb on + strand, within GFF46 at 36.547 kb on + strand, within GFF46 at 36.574 kb on + strand, within GFF46 at 36.603 kb on - strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.622 kb on + strand, within GFF46 at 36.625 kb on - strand, within GFF46 at 36.630 kb on - strand, within GFF46 at 36.630 kb on - strand, within GFF46 at 36.636 kb on + strand, within GFF46 at 36.636 kb on + strand, within GFF46 at 36.644 kb on - strand, within GFF46 at 36.644 kb on - strand, within GFF46 at 36.688 kb on + strand, within GFF46 at 36.688 kb on + strand, within GFF46 at 36.692 kb on + strand, within GFF46 at 36.694 kb on + strand, within GFF46 at 36.699 kb on + strand, within GFF46 at 36.707 kb on - strand, within GFF46 at 36.712 kb on - strand, within GFF46 at 36.777 kb on + strand, within GFF46 at 36.795 kb on - strand, within GFF46 at 36.795 kb on - strand, within GFF46 at 36.802 kb on + strand, within GFF46 at 36.802 kb on - strand, within GFF46 at 36.921 kb on + strand, within GFF46 at 36.943 kb on + strand, within GFF46 at 36.962 kb on + strand, within GFF46 at 36.985 kb on - strand, within GFF46 at 36.985 kb on - strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.009 kb on + strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.017 kb on - strand, within GFF46 at 37.034 kb on - strand, within GFF46 at 37.041 kb on - strand, within GFF46 at 37.044 kb on - strand, within GFF46 at 37.077 kb on - strand, within GFF46 at 37.080 kb on - strand, within GFF46 at 37.130 kb on - strand, within GFF46 at 37.132 kb on - strand, within GFF46 at 37.135 kb on - strand, within GFF46 at 37.142 kb on + strand, within GFF46 at 37.166 kb on + strand, within GFF46 at 37.177 kb on + strand, within GFF46 at 37.182 kb on + strand, within GFF46 at 37.186 kb on + strand, within GFF46 at 37.194 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46 at 37.204 kb on - strand, within GFF46
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 33,696 + GFF43 0.40 -1.1 33,699 - GFF43 0.41 -0.4 33,699 - GFF43 0.41 +0.4 33,708 + GFF43 0.42 -0.3 33,718 + GFF43 0.44 +0.7 33,718 + GFF43 0.44 -0.6 33,739 - GFF43 0.47 -0.1 33,769 + GFF43 0.52 -0.2 33,782 - GFF43 0.54 +0.8 33,852 - GFF43 0.65 -0.0 33,852 - GFF43 0.65 -1.0 33,852 - GFF43 0.65 +1.3 33,855 - GFF43 0.66 -0.4 33,867 + GFF43 0.68 +0.9 33,867 + GFF43 0.68 +0.7 33,875 - GFF43 0.69 +0.9 33,930 + GFF43 0.78 -1.0 33,969 - GFF43 0.84 -0.4 33,990 - GFF43 0.87 +1.7 34,013 + -1.2 34,015 + -0.3 34,030 + +0.1 34,030 + +1.3 34,034 - -0.7 34,059 + +0.9 34,059 + -1.0 34,059 + -1.1 34,067 - +0.9 34,072 - +0.8 34,072 - +0.7 34,072 - +0.9 34,072 - +0.8 34,072 - +0.5 34,072 - -0.8 34,072 - +0.5 34,077 - -2.0 34,079 - -0.6 34,089 - -0.2 34,096 - +0.0 34,129 + +1.6 34,129 + -0.8 34,137 - +1.4 34,138 + +1.8 34,145 - +1.0 34,166 - -1.6 34,166 - -0.7 34,171 - -0.5 34,171 - +0.4 34,176 - +0.2 34,206 + +0.5 34,210 - +0.0 34,214 + -0.1 34,217 - GFF44 0.10 -0.3 34,217 - GFF44 0.10 -0.1 34,217 - GFF44 0.10 -0.3 34,227 - GFF44 0.12 -0.2 34,263 + GFF44 0.20 -0.3 34,271 - GFF44 0.22 -0.7 34,312 + GFF44 0.31 +0.4 34,323 + GFF44 0.33 -0.7 34,364 - GFF44 0.42 +0.2 34,366 - GFF44 0.42 +0.6 34,366 - GFF44 0.42 +0.0 34,377 - GFF44 0.45 +1.6 34,384 - GFF44 0.46 +1.0 34,442 + GFF44 0.58 +0.6 34,442 + GFF44 0.58 -0.7 34,442 + GFF44 0.58 +0.6 34,442 + GFF44 0.58 +0.8 34,442 + GFF44 0.58 -0.5 34,450 - GFF44 0.60 -1.1 34,450 - GFF44 0.60 +0.8 34,450 - GFF44 0.60 +0.2 34,455 - GFF44 0.61 -0.8 34,464 + GFF44 0.63 -0.3 34,464 + GFF44 0.63 -2.2 34,511 + GFF44 0.73 +0.3 34,511 + GFF44 0.73 -1.1 34,519 - GFF44 0.75 -1.2 34,525 + GFF44 0.76 -1.0 34,546 - GFF44 0.81 -2.5 34,670 - +0.1 34,708 + +0.2 34,708 + +0.4 34,708 + -0.0 34,708 + +0.8 34,716 - +0.2 34,716 - +0.8 34,730 + +0.3 34,738 - -1.6 34,739 - -0.2 34,775 - -0.1 34,800 + +0.1 34,800 + +1.0 34,800 + +0.9 34,808 - -1.1 34,826 + -0.0 34,834 - -0.6 34,834 - -1.2 34,834 - -0.2 34,834 - +0.1 34,846 - +0.2 34,879 + GFF45 0.12 -1.6 34,879 - GFF45 0.12 -0.1 34,903 + GFF45 0.13 -2.3 35,009 + GFF45 0.20 -1.0 35,009 + GFF45 0.20 -0.3 35,009 + GFF45 0.20 -1.4 35,017 - GFF45 0.21 -1.7 35,034 + GFF45 0.22 +1.4 35,042 - GFF45 0.22 +0.3 35,060 + GFF45 0.24 +1.2 35,060 + GFF45 0.24 -3.2 35,068 + GFF45 0.24 +0.4 35,068 - GFF45 0.24 -1.2 35,068 - GFF45 0.24 -1.2 35,068 - GFF45 0.24 +0.3 35,068 - GFF45 0.24 +0.3 35,076 - GFF45 0.25 +1.7 35,076 - GFF45 0.25 -1.4 35,077 + GFF45 0.25 +0.6 35,079 + GFF45 0.25 -2.2 35,142 + GFF45 0.29 -2.0 35,158 + GFF45 0.30 +0.1 35,166 + GFF45 0.30 +0.0 35,168 + GFF45 0.31 -0.0 35,193 + GFF45 0.32 -0.1 35,194 + GFF45 0.32 -0.3 35,218 - GFF45 0.34 -0.3 35,236 + GFF45 0.35 +0.6 35,244 - GFF45 0.35 -0.5 35,283 + GFF45 0.38 +0.5 35,288 + GFF45 0.38 +0.7 35,291 - GFF45 0.38 +1.0 35,296 - GFF45 0.39 -0.1 35,296 - GFF45 0.39 -0.3 35,359 - GFF45 0.43 +0.5 35,375 - GFF45 0.44 -2.2 35,392 - GFF45 0.45 -0.3 35,421 + GFF45 0.47 -1.7 35,566 + GFF45 0.56 +0.2 35,566 + GFF45 0.56 +0.7 35,566 + GFF45 0.56 -0.5 35,581 + GFF45 0.57 -1.5 35,607 - GFF45 0.59 +0.4 35,614 + GFF45 0.59 -0.0 35,619 + GFF45 0.60 +1.4 35,619 + GFF45 0.60 +0.2 35,622 - GFF45 0.60 +0.1 35,647 + GFF45 0.61 +0.2 35,653 - GFF45 0.62 +0.2 35,653 - GFF45 0.62 -0.2 35,655 - GFF45 0.62 -0.0 35,700 - GFF45 0.65 -0.6 35,700 - GFF45 0.65 -0.6 35,732 + GFF45 0.67 -2.6 35,740 - GFF45 0.67 -0.9 35,766 + GFF45 0.69 -0.4 35,767 - GFF45 0.69 +0.8 35,774 - GFF45 0.70 +1.0 35,774 - GFF45 0.70 -1.0 35,821 + GFF45 0.73 -0.9 35,821 + GFF45 0.73 -0.1 35,821 + GFF45 0.73 -1.0 35,821 + GFF45 0.73 -0.6 35,821 + GFF45 0.73 -0.6 35,821 + GFF45 0.73 +2.0 35,821 + GFF45 0.73 +0.2 35,821 + GFF45 0.73 +0.4 35,821 + GFF45 0.73 -1.0 35,829 - GFF45 0.73 +0.5 35,829 - GFF45 0.73 -1.0 35,829 - GFF45 0.73 -0.5 35,829 - GFF45 0.73 +0.9 35,829 - GFF45 0.73 -0.7 35,829 - GFF45 0.73 +1.4 35,829 - GFF45 0.73 -0.4 35,848 + GFF45 0.74 +0.3 35,850 + GFF45 0.75 +1.4 35,850 + GFF45 0.75 +0.2 35,858 - GFF45 0.75 -0.0 35,863 - GFF45 0.75 -0.0 35,865 + GFF45 0.75 -0.5 35,865 + GFF45 0.75 +1.2 35,865 + GFF45 0.75 +0.7 35,865 + GFF45 0.75 -2.7 35,875 + GFF45 0.76 -0.6 35,875 + GFF45 0.76 +1.0 35,883 - GFF45 0.77 -1.3 35,883 - GFF45 0.77 -0.0 35,887 + GFF45 0.77 -2.5 35,901 + GFF45 0.78 -1.0 35,901 - GFF45 0.78 -0.4 35,909 - GFF45 0.78 -1.0 35,994 + GFF45 0.84 +0.5 36,035 + GFF45 0.86 +0.8 36,085 + GFF45 0.90 +0.4 36,102 + +0.2 36,129 + +1.2 36,129 + +1.4 36,129 + +0.5 36,129 + -2.4 36,129 + -0.7 36,129 + +0.2 36,129 + -0.1 36,137 - +0.4 36,137 - +0.2 36,137 - -0.8 36,137 - +1.2 36,146 + -1.3 36,154 - +0.5 36,224 - -0.6 36,253 + -0.0 36,261 - +1.0 36,288 + -0.4 36,294 + +1.4 36,294 + +0.2 36,308 + +0.2 36,308 + +2.0 36,316 - -0.1 36,324 - -0.1 36,356 - +0.7 36,419 + GFF46 0.10 +0.4 36,427 - GFF46 0.11 +0.2 36,449 + GFF46 0.12 -1.0 36,478 + GFF46 0.15 +0.9 36,487 - GFF46 0.15 +0.1 36,495 + GFF46 0.16 -1.2 36,547 + GFF46 0.20 +0.9 36,574 + GFF46 0.22 -0.4 36,603 - GFF46 0.24 -0.6 36,622 + GFF46 0.26 -0.3 36,622 + GFF46 0.26 +0.8 36,622 + GFF46 0.26 +0.3 36,622 + GFF46 0.26 -1.0 36,625 - GFF46 0.26 +0.1 36,630 - GFF46 0.27 +1.0 36,630 - GFF46 0.27 -0.6 36,636 + GFF46 0.27 -0.6 36,636 + GFF46 0.27 -0.6 36,644 - GFF46 0.28 -0.3 36,644 - GFF46 0.28 +0.0 36,688 + GFF46 0.31 -1.2 36,688 + GFF46 0.31 +1.8 36,692 + GFF46 0.31 -0.3 36,694 + GFF46 0.32 +0.7 36,699 + GFF46 0.32 -0.8 36,707 - GFF46 0.33 -0.2 36,712 - GFF46 0.33 +0.1 36,777 + GFF46 0.38 +0.2 36,795 - GFF46 0.39 +0.3 36,795 - GFF46 0.39 -0.6 36,802 + GFF46 0.40 -1.0 36,802 - GFF46 0.40 -1.2 36,921 + GFF46 0.49 +0.0 36,943 + GFF46 0.51 -0.3 36,962 + GFF46 0.52 -0.2 36,985 - GFF46 0.54 -2.4 36,985 - GFF46 0.54 -1.5 37,009 + GFF46 0.56 -0.2 37,009 + GFF46 0.56 +0.9 37,009 + GFF46 0.56 -0.7 37,017 - GFF46 0.57 -0.2 37,017 - GFF46 0.57 +1.4 37,017 - GFF46 0.57 -1.5 37,017 - GFF46 0.57 -0.1 37,017 - GFF46 0.57 +1.4 37,017 - GFF46 0.57 -0.1 37,034 - GFF46 0.58 +0.1 37,041 - GFF46 0.59 +0.3 37,044 - GFF46 0.59 -0.3 37,077 - GFF46 0.61 +0.1 37,080 - GFF46 0.62 -0.2 37,130 - GFF46 0.66 +0.4 37,132 - GFF46 0.66 -1.0 37,135 - GFF46 0.66 -1.0 37,142 + GFF46 0.67 +1.8 37,166 + GFF46 0.68 +1.2 37,177 + GFF46 0.69 +1.2 37,182 + GFF46 0.70 -0.5 37,186 + GFF46 0.70 +1.1 37,194 - GFF46 0.71 +0.5 37,204 - GFF46 0.71 +1.1 37,204 - GFF46 0.71 +2.2
Or see this region's nucleotide sequence