Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3927 and GFF3928 are separated by 115 nucleotides GFF3928 and GFF3929 are separated by 189 nucleotides GFF3929 and GFF3930 overlap by 1 nucleotides GFF3930 and GFF3931 are separated by 41 nucleotides
GFF3927 - hypothetical protein, at 3,966,723 to 3,966,968
GFF3927
GFF3928 - UspA, at 3,967,084 to 3,967,539
GFF3928
GFF3929 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 3,967,729 to 3,969,033
GFF3929
GFF3930 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 3,969,033 to 3,969,434
GFF3930
GFF3931 - Dihydrolipoamide dehydrogenase of pyruvate dehydrogenase complex (EC 1.8.1.4), at 3,969,476 to 3,970,891
GFF3931
Position (kb)
3967
3968
3969
3970 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3966.768 kb on + strand, within GFF3927 at 3966.853 kb on - strand, within GFF3927 at 3966.899 kb on + strand, within GFF3927 at 3966.902 kb on - strand, within GFF3927 at 3966.904 kb on + strand, within GFF3927 at 3966.904 kb on + strand, within GFF3927 at 3966.904 kb on + strand, within GFF3927 at 3966.907 kb on - strand, within GFF3927 at 3966.911 kb on + strand, within GFF3927 at 3966.912 kb on - strand, within GFF3927 at 3966.912 kb on - strand, within GFF3927 at 3966.912 kb on - strand, within GFF3927 at 3966.912 kb on - strand, within GFF3927 at 3966.924 kb on + strand, within GFF3927 at 3966.926 kb on + strand, within GFF3927 at 3966.961 kb on + strand at 3966.961 kb on + strand at 3966.969 kb on - strand at 3967.005 kb on + strand at 3967.006 kb on - strand at 3967.006 kb on - strand at 3967.013 kb on - strand at 3967.013 kb on - strand at 3967.062 kb on + strand at 3967.068 kb on + strand at 3967.068 kb on + strand at 3967.068 kb on + strand at 3967.068 kb on + strand at 3967.076 kb on - strand at 3967.076 kb on - strand at 3967.082 kb on + strand at 3967.083 kb on - strand at 3967.085 kb on - strand at 3967.093 kb on - strand at 3967.111 kb on + strand at 3967.111 kb on + strand at 3967.121 kb on + strand at 3967.134 kb on + strand, within GFF3928 at 3967.158 kb on + strand, within GFF3928 at 3967.158 kb on + strand, within GFF3928 at 3967.173 kb on + strand, within GFF3928 at 3967.181 kb on - strand, within GFF3928 at 3967.185 kb on - strand, within GFF3928 at 3967.192 kb on + strand, within GFF3928 at 3967.200 kb on - strand, within GFF3928 at 3967.214 kb on + strand, within GFF3928 at 3967.214 kb on + strand, within GFF3928 at 3967.218 kb on + strand, within GFF3928 at 3967.218 kb on + strand, within GFF3928 at 3967.219 kb on + strand, within GFF3928 at 3967.221 kb on - strand, within GFF3928 at 3967.226 kb on - strand, within GFF3928 at 3967.227 kb on - strand, within GFF3928 at 3967.227 kb on - strand, within GFF3928 at 3967.227 kb on - strand, within GFF3928 at 3967.254 kb on - strand, within GFF3928 at 3967.258 kb on + strand, within GFF3928 at 3967.261 kb on - strand, within GFF3928 at 3967.274 kb on + strand, within GFF3928 at 3967.274 kb on + strand, within GFF3928 at 3967.290 kb on + strand, within GFF3928 at 3967.298 kb on - strand, within GFF3928 at 3967.298 kb on - strand, within GFF3928 at 3967.361 kb on + strand, within GFF3928 at 3967.370 kb on + strand, within GFF3928 at 3967.370 kb on + strand, within GFF3928 at 3967.370 kb on + strand, within GFF3928 at 3967.378 kb on - strand, within GFF3928 at 3967.378 kb on - strand, within GFF3928 at 3967.378 kb on - strand, within GFF3928 at 3967.405 kb on - strand, within GFF3928 at 3967.444 kb on + strand, within GFF3928 at 3967.449 kb on + strand, within GFF3928 at 3967.478 kb on + strand, within GFF3928 at 3967.478 kb on + strand, within GFF3928 at 3967.486 kb on - strand, within GFF3928 at 3967.486 kb on - strand, within GFF3928 at 3967.581 kb on + strand at 3967.589 kb on - strand at 3967.589 kb on - strand at 3967.599 kb on - strand at 3967.624 kb on + strand at 3967.633 kb on - strand at 3967.636 kb on + strand at 3967.649 kb on + strand at 3967.649 kb on + strand at 3967.649 kb on + strand at 3967.649 kb on + strand at 3967.649 kb on + strand at 3967.649 kb on + strand at 3967.652 kb on - strand at 3967.656 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.657 kb on - strand at 3967.753 kb on + strand at 3967.766 kb on + strand at 3967.865 kb on - strand, within GFF3929 at 3967.969 kb on + strand, within GFF3929 at 3968.032 kb on + strand, within GFF3929 at 3968.181 kb on + strand, within GFF3929 at 3968.243 kb on + strand, within GFF3929 at 3968.261 kb on - strand, within GFF3929 at 3968.422 kb on + strand, within GFF3929 at 3968.721 kb on + strand, within GFF3929 at 3968.828 kb on - strand, within GFF3929 at 3969.018 kb on + strand at 3969.026 kb on - strand at 3969.030 kb on - strand at 3969.060 kb on + strand at 3969.062 kb on + strand at 3969.067 kb on + strand at 3969.070 kb on - strand at 3969.070 kb on - strand at 3969.075 kb on - strand, within GFF3930 at 3969.075 kb on - strand, within GFF3930 at 3969.075 kb on - strand, within GFF3930 at 3969.075 kb on - strand, within GFF3930 at 3969.075 kb on - strand, within GFF3930 at 3969.140 kb on - strand, within GFF3930 at 3969.150 kb on - strand, within GFF3930 at 3969.190 kb on + strand, within GFF3930 at 3969.193 kb on + strand, within GFF3930 at 3969.198 kb on - strand, within GFF3930 at 3969.215 kb on + strand, within GFF3930 at 3969.215 kb on + strand, within GFF3930 at 3969.215 kb on + strand, within GFF3930 at 3969.215 kb on + strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.223 kb on - strand, within GFF3930 at 3969.230 kb on - strand, within GFF3930 at 3969.255 kb on + strand, within GFF3930 at 3969.255 kb on + strand, within GFF3930 at 3969.255 kb on - strand, within GFF3930 at 3969.263 kb on - strand, within GFF3930 at 3969.263 kb on - strand, within GFF3930 at 3969.263 kb on - strand, within GFF3930 at 3969.269 kb on - strand, within GFF3930 at 3969.300 kb on - strand, within GFF3930 at 3969.300 kb on - strand, within GFF3930 at 3969.310 kb on - strand, within GFF3930 at 3969.339 kb on + strand, within GFF3930 at 3969.346 kb on - strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.366 kb on + strand, within GFF3930 at 3969.374 kb on - strand, within GFF3930 at 3969.374 kb on - strand, within GFF3930 at 3969.374 kb on - strand, within GFF3930 at 3969.374 kb on - strand, within GFF3930 at 3969.374 kb on - strand, within GFF3930 at 3969.381 kb on - strand, within GFF3930 at 3969.396 kb on + strand at 3969.400 kb on - strand at 3969.409 kb on - strand at 3969.414 kb on - strand at 3969.416 kb on + strand at 3969.429 kb on + strand at 3969.437 kb on - strand at 3969.454 kb on - strand at 3969.454 kb on - strand at 3969.454 kb on - strand at 3969.454 kb on - strand at 3969.462 kb on + strand at 3969.462 kb on + strand at 3969.467 kb on + strand at 3969.470 kb on - strand at 3969.486 kb on + strand at 3969.507 kb on + strand at 3969.507 kb on + strand at 3969.530 kb on - strand at 3969.548 kb on + strand at 3969.567 kb on + strand at 3969.570 kb on - strand at 3969.642 kb on - strand, within GFF3931 at 3969.676 kb on + strand, within GFF3931 at 3969.708 kb on - strand, within GFF3931 at 3969.722 kb on + strand, within GFF3931 at 3969.732 kb on - strand, within GFF3931 at 3969.840 kb on - strand, within GFF3931 at 3969.840 kb on - strand, within GFF3931 at 3969.855 kb on - strand, within GFF3931 at 3969.876 kb on + strand, within GFF3931 at 3969.877 kb on - strand, within GFF3931 at 3969.884 kb on - strand, within GFF3931 at 3969.891 kb on - strand, within GFF3931 at 3969.976 kb on - strand, within GFF3931 at 3969.977 kb on - strand, within GFF3931 at 3970.024 kb on - strand, within GFF3931
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 3,966,768 + GFF3927 0.18 -1.6 3,966,853 - GFF3927 0.53 +2.1 3,966,899 + GFF3927 0.72 -1.4 3,966,902 - GFF3927 0.73 -1.4 3,966,904 + GFF3927 0.74 -0.6 3,966,904 + GFF3927 0.74 -1.6 3,966,904 + GFF3927 0.74 +0.6 3,966,907 - GFF3927 0.75 -0.6 3,966,911 + GFF3927 0.76 -2.5 3,966,912 - GFF3927 0.77 -0.3 3,966,912 - GFF3927 0.77 +0.6 3,966,912 - GFF3927 0.77 +0.1 3,966,912 - GFF3927 0.77 -0.9 3,966,924 + GFF3927 0.82 +0.4 3,966,926 + GFF3927 0.83 -2.4 3,966,961 + -2.9 3,966,961 + +0.1 3,966,969 - -0.8 3,967,005 + +0.2 3,967,006 - +0.6 3,967,006 - +0.6 3,967,013 - +0.7 3,967,013 - -0.4 3,967,062 + -0.8 3,967,068 + -0.1 3,967,068 + +0.1 3,967,068 + +1.0 3,967,068 + +0.4 3,967,076 - -0.1 3,967,076 - +0.1 3,967,082 + -1.6 3,967,083 - -1.4 3,967,085 - -0.9 3,967,093 - -0.6 3,967,111 + +0.6 3,967,111 + +1.0 3,967,121 + +0.4 3,967,134 + GFF3928 0.11 -0.1 3,967,158 + GFF3928 0.16 -0.2 3,967,158 + GFF3928 0.16 -0.3 3,967,173 + GFF3928 0.20 -0.6 3,967,181 - GFF3928 0.21 -0.5 3,967,185 - GFF3928 0.22 -0.1 3,967,192 + GFF3928 0.24 -0.6 3,967,200 - GFF3928 0.25 -0.5 3,967,214 + GFF3928 0.29 -0.2 3,967,214 + GFF3928 0.29 +0.9 3,967,218 + GFF3928 0.29 +0.2 3,967,218 + GFF3928 0.29 +0.2 3,967,219 + GFF3928 0.30 +0.1 3,967,221 - GFF3928 0.30 -0.2 3,967,226 - GFF3928 0.31 +1.2 3,967,227 - GFF3928 0.31 -0.8 3,967,227 - GFF3928 0.31 -0.8 3,967,227 - GFF3928 0.31 +0.0 3,967,254 - GFF3928 0.37 -1.6 3,967,258 + GFF3928 0.38 -1.0 3,967,261 - GFF3928 0.39 -0.5 3,967,274 + GFF3928 0.42 +1.0 3,967,274 + GFF3928 0.42 +0.6 3,967,290 + GFF3928 0.45 -0.6 3,967,298 - GFF3928 0.47 -2.8 3,967,298 - GFF3928 0.47 +0.6 3,967,361 + GFF3928 0.61 -0.2 3,967,370 + GFF3928 0.63 -0.1 3,967,370 + GFF3928 0.63 -3.1 3,967,370 + GFF3928 0.63 -0.2 3,967,378 - GFF3928 0.64 -1.9 3,967,378 - GFF3928 0.64 +0.8 3,967,378 - GFF3928 0.64 -1.1 3,967,405 - GFF3928 0.70 -0.6 3,967,444 + GFF3928 0.79 -1.2 3,967,449 + GFF3928 0.80 -1.2 3,967,478 + GFF3928 0.86 +0.1 3,967,478 + GFF3928 0.86 -0.9 3,967,486 - GFF3928 0.88 -0.9 3,967,486 - GFF3928 0.88 +0.2 3,967,581 + -1.3 3,967,589 - -0.2 3,967,589 - -1.4 3,967,599 - -0.1 3,967,624 + -1.7 3,967,633 - -0.6 3,967,636 + -3.3 3,967,649 + -0.2 3,967,649 + -1.5 3,967,649 + -0.8 3,967,649 + +1.2 3,967,649 + -0.2 3,967,649 + -0.3 3,967,652 - -0.1 3,967,656 - -1.0 3,967,657 - -0.2 3,967,657 - -1.7 3,967,657 - -0.4 3,967,657 - -0.2 3,967,657 - -0.5 3,967,657 - +0.3 3,967,657 - +0.1 3,967,657 - +0.2 3,967,753 + -0.7 3,967,766 + -2.0 3,967,865 - GFF3929 0.10 -1.8 3,967,969 + GFF3929 0.18 -2.1 3,968,032 + GFF3929 0.23 -0.6 3,968,181 + GFF3929 0.35 +0.0 3,968,243 + GFF3929 0.39 -1.7 3,968,261 - GFF3929 0.41 -2.4 3,968,422 + GFF3929 0.53 -1.7 3,968,721 + GFF3929 0.76 -1.8 3,968,828 - GFF3929 0.84 -2.0 3,969,018 + -0.6 3,969,026 - -1.4 3,969,030 - -2.1 3,969,060 + -0.9 3,969,062 + +0.9 3,969,067 + -0.5 3,969,070 - -0.3 3,969,070 - +0.4 3,969,075 - GFF3930 0.10 -1.6 3,969,075 - GFF3930 0.10 -2.0 3,969,075 - GFF3930 0.10 -1.1 3,969,075 - GFF3930 0.10 -2.1 3,969,075 - GFF3930 0.10 -1.6 3,969,140 - GFF3930 0.27 -1.2 3,969,150 - GFF3930 0.29 -1.4 3,969,190 + GFF3930 0.39 -0.3 3,969,193 + GFF3930 0.40 +0.6 3,969,198 - GFF3930 0.41 -2.2 3,969,215 + GFF3930 0.45 +0.6 3,969,215 + GFF3930 0.45 +0.2 3,969,215 + GFF3930 0.45 -0.1 3,969,215 + GFF3930 0.45 -0.5 3,969,223 - GFF3930 0.47 -3.7 3,969,223 - GFF3930 0.47 -1.6 3,969,223 - GFF3930 0.47 +0.1 3,969,223 - GFF3930 0.47 -0.1 3,969,223 - GFF3930 0.47 +0.1 3,969,223 - GFF3930 0.47 -2.8 3,969,223 - GFF3930 0.47 -1.4 3,969,223 - GFF3930 0.47 +0.1 3,969,223 - GFF3930 0.47 -1.2 3,969,230 - GFF3930 0.49 -2.3 3,969,255 + GFF3930 0.55 +0.7 3,969,255 + GFF3930 0.55 +0.0 3,969,255 - GFF3930 0.55 -2.3 3,969,263 - GFF3930 0.57 -1.9 3,969,263 - GFF3930 0.57 -1.9 3,969,263 - GFF3930 0.57 +0.5 3,969,269 - GFF3930 0.59 -2.9 3,969,300 - GFF3930 0.66 +0.9 3,969,300 - GFF3930 0.66 +0.9 3,969,310 - GFF3930 0.69 -0.3 3,969,339 + GFF3930 0.76 +1.4 3,969,346 - GFF3930 0.78 -1.2 3,969,366 + GFF3930 0.83 -2.2 3,969,366 + GFF3930 0.83 +0.4 3,969,366 + GFF3930 0.83 +0.4 3,969,366 + GFF3930 0.83 +0.1 3,969,366 + GFF3930 0.83 -0.5 3,969,366 + GFF3930 0.83 +0.7 3,969,366 + GFF3930 0.83 +0.9 3,969,366 + GFF3930 0.83 -0.6 3,969,366 + GFF3930 0.83 -0.6 3,969,374 - GFF3930 0.85 -1.0 3,969,374 - GFF3930 0.85 -0.2 3,969,374 - GFF3930 0.85 -0.9 3,969,374 - GFF3930 0.85 -1.0 3,969,374 - GFF3930 0.85 -0.1 3,969,381 - GFF3930 0.87 -2.4 3,969,396 + +0.6 3,969,400 - -0.9 3,969,409 - -3.0 3,969,414 - -0.8 3,969,416 + +1.2 3,969,429 + +0.3 3,969,437 - -2.5 3,969,454 - -3.0 3,969,454 - -1.6 3,969,454 - -2.1 3,969,454 - -0.6 3,969,462 + -0.3 3,969,462 + -0.8 3,969,467 + -0.6 3,969,470 - -2.8 3,969,486 + -0.2 3,969,507 + -0.8 3,969,507 + -1.6 3,969,530 - -0.6 3,969,548 + -2.6 3,969,567 + -1.8 3,969,570 - -1.7 3,969,642 - GFF3931 0.12 -0.8 3,969,676 + GFF3931 0.14 -1.9 3,969,708 - GFF3931 0.16 -1.9 3,969,722 + GFF3931 0.17 -1.2 3,969,732 - GFF3931 0.18 -2.5 3,969,840 - GFF3931 0.26 -1.4 3,969,840 - GFF3931 0.26 -1.1 3,969,855 - GFF3931 0.27 -0.6 3,969,876 + GFF3931 0.28 +0.1 3,969,877 - GFF3931 0.28 -1.4 3,969,884 - GFF3931 0.29 -2.4 3,969,891 - GFF3931 0.29 -2.9 3,969,976 - GFF3931 0.35 -2.0 3,969,977 - GFF3931 0.35 -2.1 3,970,024 - GFF3931 0.39 -1.4
Or see this region's nucleotide sequence