Experiment: EMM_noCarbon with D-Glucose and Ammonium Sulfate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF219 and GFF220 are separated by 83 nucleotides GFF220 and GFF221 are separated by 56 nucleotides GFF221 and GFF222 are separated by 183 nucleotides GFF222 and GFF223 are separated by 180 nucleotides GFF223 and GFF224 are separated by 154 nucleotides GFF224 and GFF225 are separated by 75 nucleotides
GFF219 - hypothetical protein, at 226,587 to 226,805
GFF219
GFF220 - hypothetical protein, at 226,889 to 227,122
GFF220
GFF221 - hypothetical protein, at 227,179 to 227,604
GFF221
GFF222 - hypothetical protein, at 227,788 to 228,771
GFF222
GFF223 - LSU ribosomal protein L28p @ LSU ribosomal protein L28p, zinc-independent, at 228,952 to 229,245
GFF223
GFF224 - hypothetical protein, at 229,400 to 229,621
GFF224
GFF225 - tRNA-specific adenosine-34 deaminase (EC 3.5.4.33), at 229,697 to 230,155
GFF225
Position (kb) 227
228
229 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 226.843 kb on - strand at 226.861 kb on + strand at 226.887 kb on - strand at 226.904 kb on + strand at 226.907 kb on - strand at 226.907 kb on - strand at 226.920 kb on - strand, within GFF220 at 226.933 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.990 kb on + strand, within GFF220 at 226.993 kb on + strand, within GFF220 at 227.001 kb on - strand, within GFF220 at 227.060 kb on - strand, within GFF220 at 227.061 kb on - strand, within GFF220 at 227.068 kb on + strand, within GFF220 at 227.085 kb on - strand, within GFF220 at 227.104 kb on + strand at 227.112 kb on - strand at 227.152 kb on + strand at 227.160 kb on - strand at 227.160 kb on - strand at 227.170 kb on + strand at 227.175 kb on - strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.270 kb on + strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.286 kb on + strand, within GFF221 at 227.294 kb on - strand, within GFF221 at 227.301 kb on + strand, within GFF221 at 227.326 kb on + strand, within GFF221 at 227.326 kb on - strand, within GFF221 at 227.334 kb on - strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.343 kb on + strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.437 kb on - strand, within GFF221 at 227.444 kb on + strand, within GFF221 at 227.458 kb on - strand, within GFF221 at 227.474 kb on + strand, within GFF221 at 227.474 kb on + strand, within GFF221 at 227.482 kb on - strand, within GFF221 at 227.490 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.511 kb on - strand, within GFF221 at 227.511 kb on - strand, within GFF221 at 227.517 kb on - strand, within GFF221 at 227.549 kb on - strand, within GFF221 at 227.553 kb on - strand, within GFF221 at 227.553 kb on - strand, within GFF221 at 227.558 kb on + strand, within GFF221 at 227.566 kb on - strand at 227.576 kb on - strand at 227.587 kb on - strand at 227.606 kb on + strand at 227.614 kb on - strand at 227.619 kb on - strand at 227.637 kb on + strand at 227.669 kb on + strand at 227.669 kb on + strand at 227.677 kb on - strand at 227.718 kb on + strand at 227.726 kb on - strand at 227.726 kb on - strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.750 kb on + strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.779 kb on - strand at 227.792 kb on + strand at 227.792 kb on + strand at 227.792 kb on + strand at 227.800 kb on - strand at 227.800 kb on - strand at 227.826 kb on + strand at 227.834 kb on - strand at 227.847 kb on + strand at 227.847 kb on + strand at 227.868 kb on + strand at 227.868 kb on + strand at 227.868 kb on + strand at 227.880 kb on + strand at 227.883 kb on + strand at 227.905 kb on + strand, within GFF222 at 227.907 kb on + strand, within GFF222 at 227.913 kb on + strand, within GFF222 at 227.946 kb on + strand, within GFF222 at 227.954 kb on - strand, within GFF222 at 227.976 kb on + strand, within GFF222 at 228.020 kb on - strand, within GFF222 at 228.025 kb on + strand, within GFF222 at 228.037 kb on + strand, within GFF222 at 228.047 kb on + strand, within GFF222 at 228.047 kb on + strand, within GFF222 at 228.050 kb on - strand, within GFF222 at 228.057 kb on + strand, within GFF222 at 228.073 kb on - strand, within GFF222 at 228.093 kb on + strand, within GFF222 at 228.100 kb on - strand, within GFF222 at 228.127 kb on + strand, within GFF222 at 228.127 kb on + strand, within GFF222 at 228.132 kb on + strand, within GFF222 at 228.132 kb on + strand, within GFF222 at 228.132 kb on + strand, within GFF222 at 228.132 kb on + strand, within GFF222 at 228.135 kb on - strand, within GFF222 at 228.140 kb on - strand, within GFF222 at 228.147 kb on - strand, within GFF222 at 228.151 kb on - strand, within GFF222 at 228.153 kb on + strand, within GFF222 at 228.176 kb on + strand, within GFF222 at 228.206 kb on - strand, within GFF222 at 228.208 kb on - strand, within GFF222 at 228.209 kb on + strand, within GFF222 at 228.209 kb on + strand, within GFF222 at 228.209 kb on + strand, within GFF222 at 228.209 kb on + strand, within GFF222 at 228.210 kb on + strand, within GFF222 at 228.217 kb on - strand, within GFF222 at 228.217 kb on - strand, within GFF222 at 228.217 kb on - strand, within GFF222 at 228.217 kb on - strand, within GFF222 at 228.217 kb on - strand, within GFF222 at 228.219 kb on + strand, within GFF222 at 228.227 kb on - strand, within GFF222 at 228.227 kb on - strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.258 kb on + strand, within GFF222 at 228.266 kb on - strand, within GFF222 at 228.266 kb on - strand, within GFF222 at 228.266 kb on - strand, within GFF222 at 228.266 kb on - strand, within GFF222 at 228.267 kb on + strand, within GFF222 at 228.267 kb on - strand, within GFF222 at 228.276 kb on - strand, within GFF222 at 228.297 kb on + strand, within GFF222 at 228.305 kb on - strand, within GFF222 at 228.310 kb on - strand, within GFF222 at 228.366 kb on + strand, within GFF222 at 228.366 kb on + strand, within GFF222 at 228.366 kb on + strand, within GFF222 at 228.374 kb on - strand, within GFF222 at 228.374 kb on - strand, within GFF222 at 228.404 kb on + strand, within GFF222 at 228.404 kb on + strand, within GFF222 at 228.431 kb on + strand, within GFF222 at 228.450 kb on + strand, within GFF222 at 228.458 kb on - strand, within GFF222 at 228.458 kb on - strand, within GFF222 at 228.470 kb on + strand, within GFF222 at 228.470 kb on + strand, within GFF222 at 228.471 kb on - strand, within GFF222 at 228.476 kb on - strand, within GFF222 at 228.491 kb on - strand, within GFF222 at 228.496 kb on - strand, within GFF222 at 228.501 kb on - strand, within GFF222 at 228.517 kb on + strand, within GFF222 at 228.530 kb on + strand, within GFF222 at 228.547 kb on - strand, within GFF222 at 228.547 kb on - strand, within GFF222 at 228.549 kb on + strand, within GFF222 at 228.587 kb on - strand, within GFF222 at 228.595 kb on - strand, within GFF222 at 228.595 kb on - strand, within GFF222 at 228.599 kb on + strand, within GFF222 at 228.607 kb on - strand, within GFF222 at 228.607 kb on - strand, within GFF222 at 228.649 kb on + strand, within GFF222 at 228.665 kb on + strand, within GFF222 at 228.672 kb on - strand, within GFF222 at 228.672 kb on - strand, within GFF222 at 228.673 kb on - strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.677 kb on + strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.685 kb on - strand at 228.687 kb on + strand at 228.687 kb on + strand at 228.687 kb on + strand at 228.697 kb on + strand at 228.718 kb on - strand at 228.718 kb on - strand at 228.765 kb on + strand at 228.770 kb on + strand at 228.773 kb on - strand at 228.775 kb on + strand at 228.777 kb on + strand at 228.777 kb on + strand at 228.783 kb on - strand at 228.785 kb on - strand at 228.795 kb on + strand at 228.799 kb on + strand at 228.803 kb on - strand at 228.803 kb on - strand at 228.803 kb on - strand at 228.803 kb on - strand at 228.809 kb on + strand at 228.814 kb on - strand at 228.815 kb on + strand at 228.817 kb on - strand at 228.846 kb on - strand at 228.875 kb on + strand at 229.311 kb on + strand at 229.319 kb on - strand at 229.329 kb on + strand at 229.337 kb on - strand at 229.370 kb on + strand at 229.378 kb on - strand at 229.378 kb on - strand at 229.378 kb on - strand at 229.380 kb on + strand at 229.380 kb on + strand at 229.383 kb on + strand at 229.385 kb on + strand at 229.385 kb on + strand at 229.388 kb on + strand at 229.388 kb on - strand at 229.390 kb on + strand at 229.391 kb on - strand at 229.395 kb on + strand at 229.398 kb on - strand at 229.403 kb on - strand at 229.475 kb on + strand, within GFF224 at 229.477 kb on + strand, within GFF224 at 229.477 kb on + strand, within GFF224 at 229.477 kb on + strand, within GFF224 at 229.485 kb on - strand, within GFF224 at 229.485 kb on - strand, within GFF224 at 229.513 kb on + strand, within GFF224 at 229.520 kb on - strand, within GFF224 at 229.540 kb on - strand, within GFF224 at 229.565 kb on - strand, within GFF224 at 229.566 kb on - strand, within GFF224 at 229.566 kb on - strand, within GFF224 at 229.591 kb on + strand, within GFF224 at 229.592 kb on + strand, within GFF224 at 229.615 kb on + strand at 229.618 kb on - strand at 229.623 kb on - strand at 229.623 kb on - strand at 229.679 kb on + strand at 229.679 kb on + strand at 229.679 kb on + strand at 229.681 kb on + strand at 229.681 kb on + strand at 229.681 kb on + strand at 229.687 kb on - strand at 229.689 kb on - strand at 229.689 kb on - strand at 229.693 kb on + strand at 229.693 kb on + strand at 229.694 kb on + strand at 229.694 kb on + strand at 229.694 kb on + strand at 229.695 kb on - strand at 229.695 kb on - strand at 229.700 kb on + strand at 229.701 kb on - strand at 229.702 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with D-Glucose and Ammonium Sulfate remove 226,843 - -1.0 226,861 + -1.1 226,887 - -0.3 226,904 + +1.2 226,907 - -0.4 226,907 - -0.1 226,920 - GFF220 0.13 +0.2 226,933 + GFF220 0.19 -0.2 226,948 + GFF220 0.25 +0.6 226,948 + GFF220 0.25 -0.2 226,976 + GFF220 0.37 -0.8 226,976 + GFF220 0.37 -0.9 226,976 + GFF220 0.37 -0.3 226,976 + GFF220 0.37 +0.1 226,976 + GFF220 0.37 +0.4 226,976 + GFF220 0.37 -0.5 226,976 + GFF220 0.37 -0.8 226,976 + GFF220 0.37 -2.3 226,976 + GFF220 0.37 +0.6 226,984 - GFF220 0.41 +0.6 226,984 - GFF220 0.41 -0.7 226,984 - GFF220 0.41 +0.7 226,984 - GFF220 0.41 +0.0 226,984 - GFF220 0.41 -0.4 226,984 - GFF220 0.41 -1.0 226,990 + GFF220 0.43 +1.0 226,993 + GFF220 0.44 +0.6 227,001 - GFF220 0.48 +1.0 227,060 - GFF220 0.73 +0.8 227,061 - GFF220 0.74 -0.0 227,068 + GFF220 0.76 -0.5 227,085 - GFF220 0.84 -1.1 227,104 + +0.5 227,112 - -0.2 227,152 + -0.5 227,160 - -0.9 227,160 - +0.8 227,170 + +0.6 227,175 - -1.8 227,177 + +0.3 227,177 + +2.1 227,177 + +0.7 227,185 - -0.2 227,185 - -0.6 227,185 - +0.0 227,270 + GFF221 0.21 +0.4 227,283 - GFF221 0.24 -0.9 227,283 - GFF221 0.24 -0.6 227,286 + GFF221 0.25 -1.1 227,294 - GFF221 0.27 -1.5 227,301 + GFF221 0.29 -0.4 227,326 + GFF221 0.35 -0.5 227,326 - GFF221 0.35 +0.4 227,334 - GFF221 0.36 -0.8 227,339 + GFF221 0.38 -0.4 227,339 + GFF221 0.38 -1.1 227,339 + GFF221 0.38 +0.1 227,343 + GFF221 0.38 -0.4 227,351 - GFF221 0.40 +0.6 227,351 - GFF221 0.40 -0.2 227,437 - GFF221 0.61 -0.3 227,444 + GFF221 0.62 +0.6 227,458 - GFF221 0.65 -0.9 227,474 + GFF221 0.69 -0.1 227,474 + GFF221 0.69 -2.9 227,482 - GFF221 0.71 +0.3 227,490 + GFF221 0.73 -1.7 227,503 + GFF221 0.76 +0.3 227,503 + GFF221 0.76 -0.2 227,503 + GFF221 0.76 -0.5 227,503 + GFF221 0.76 -1.5 227,503 + GFF221 0.76 -0.1 227,503 + GFF221 0.76 -0.5 227,511 - GFF221 0.78 -0.4 227,511 - GFF221 0.78 +1.4 227,517 - GFF221 0.79 -0.4 227,549 - GFF221 0.87 -0.3 227,553 - GFF221 0.88 -1.4 227,553 - GFF221 0.88 -1.0 227,558 + GFF221 0.89 +1.8 227,566 - -1.5 227,576 - +0.2 227,587 - +0.2 227,606 + -1.0 227,614 - -0.3 227,619 - +0.3 227,637 + -0.4 227,669 + -1.4 227,669 + -0.9 227,677 - +0.6 227,718 + +0.3 227,726 - +0.4 227,726 - +0.2 227,745 + +0.6 227,745 + -1.0 227,745 + -0.7 227,745 + -1.4 227,750 + -2.2 227,753 - -1.3 227,753 - -0.2 227,753 - +0.6 227,753 - -0.8 227,779 - -0.3 227,792 + -0.2 227,792 + -1.3 227,792 + -1.8 227,800 - +0.9 227,800 - +0.4 227,826 + -1.0 227,834 - -0.7 227,847 + -1.5 227,847 + +0.4 227,868 + +0.3 227,868 + -0.1 227,868 + -1.5 227,880 + +0.7 227,883 + +0.6 227,905 + GFF222 0.12 +0.4 227,907 + GFF222 0.12 -1.7 227,913 + GFF222 0.13 -0.7 227,946 + GFF222 0.16 -0.3 227,954 - GFF222 0.17 -3.2 227,976 + GFF222 0.19 -2.6 228,020 - GFF222 0.24 +0.3 228,025 + GFF222 0.24 -0.8 228,037 + GFF222 0.25 +0.2 228,047 + GFF222 0.26 +0.2 228,047 + GFF222 0.26 -1.5 228,050 - GFF222 0.27 +1.0 228,057 + GFF222 0.27 -0.3 228,073 - GFF222 0.29 -1.5 228,093 + GFF222 0.31 +0.1 228,100 - GFF222 0.32 +0.6 228,127 + GFF222 0.34 -0.3 228,127 + GFF222 0.34 -1.0 228,132 + GFF222 0.35 -0.2 228,132 + GFF222 0.35 +0.6 228,132 + GFF222 0.35 -0.0 228,132 + GFF222 0.35 +0.1 228,135 - GFF222 0.35 +0.0 228,140 - GFF222 0.36 -2.2 228,147 - GFF222 0.36 +0.5 228,151 - GFF222 0.37 -1.0 228,153 + GFF222 0.37 +0.9 228,176 + GFF222 0.39 -0.8 228,206 - GFF222 0.42 +0.8 228,208 - GFF222 0.43 -0.3 228,209 + GFF222 0.43 +0.4 228,209 + GFF222 0.43 -0.5 228,209 + GFF222 0.43 +0.6 228,209 + GFF222 0.43 -0.1 228,210 + GFF222 0.43 +0.3 228,217 - GFF222 0.44 -0.5 228,217 - GFF222 0.44 +0.8 228,217 - GFF222 0.44 +0.0 228,217 - GFF222 0.44 -0.3 228,217 - GFF222 0.44 -0.7 228,219 + GFF222 0.44 -0.8 228,227 - GFF222 0.45 +0.3 228,227 - GFF222 0.45 +1.6 228,258 + GFF222 0.48 +0.9 228,258 + GFF222 0.48 +1.0 228,258 + GFF222 0.48 -2.0 228,258 + GFF222 0.48 -2.4 228,258 + GFF222 0.48 +0.9 228,258 + GFF222 0.48 -0.4 228,266 - GFF222 0.49 +0.4 228,266 - GFF222 0.49 -1.1 228,266 - GFF222 0.49 -0.3 228,266 - GFF222 0.49 -0.0 228,267 + GFF222 0.49 +0.5 228,267 - GFF222 0.49 -2.6 228,276 - GFF222 0.50 -0.7 228,297 + GFF222 0.52 -0.8 228,305 - GFF222 0.53 -0.6 228,310 - GFF222 0.53 -0.0 228,366 + GFF222 0.59 -2.0 228,366 + GFF222 0.59 -0.0 228,366 + GFF222 0.59 +1.0 228,374 - GFF222 0.60 -0.0 228,374 - GFF222 0.60 -0.6 228,404 + GFF222 0.63 -1.2 228,404 + GFF222 0.63 +0.6 228,431 + GFF222 0.65 +1.2 228,450 + GFF222 0.67 -1.8 228,458 - GFF222 0.68 -1.0 228,458 - GFF222 0.68 -1.1 228,470 + GFF222 0.69 -1.2 228,470 + GFF222 0.69 -1.2 228,471 - GFF222 0.69 +0.6 228,476 - GFF222 0.70 -2.1 228,491 - GFF222 0.71 -0.9 228,496 - GFF222 0.72 -1.5 228,501 - GFF222 0.72 -0.5 228,517 + GFF222 0.74 +1.6 228,530 + GFF222 0.75 -0.0 228,547 - GFF222 0.77 -0.0 228,547 - GFF222 0.77 +0.4 228,549 + GFF222 0.77 +0.3 228,587 - GFF222 0.81 +0.6 228,595 - GFF222 0.82 -1.1 228,595 - GFF222 0.82 -1.8 228,599 + GFF222 0.82 +1.3 228,607 - GFF222 0.83 +0.4 228,607 - GFF222 0.83 -0.8 228,649 + GFF222 0.88 -1.6 228,665 + GFF222 0.89 +0.1 228,672 - GFF222 0.90 -0.3 228,672 - GFF222 0.90 -0.8 228,673 - -0.3 228,677 + +0.1 228,677 + -0.2 228,677 + +0.2 228,677 + +0.2 228,677 + -0.4 228,677 + +0.5 228,677 + -1.7 228,677 + -1.3 228,677 + -0.2 228,677 + -0.4 228,677 + -0.8 228,685 - +0.6 228,685 - +1.3 228,685 - -1.0 228,685 - -0.5 228,685 - -0.2 228,685 - +1.0 228,687 + -0.6 228,687 + -0.4 228,687 + -0.6 228,697 + -0.5 228,718 - -0.8 228,718 - +0.0 228,765 + +0.1 228,770 + -1.0 228,773 - -0.5 228,775 + +0.0 228,777 + -0.5 228,777 + +0.2 228,783 - +0.7 228,785 - -1.2 228,795 + -0.5 228,799 + +0.3 228,803 - -0.4 228,803 - +0.5 228,803 - +1.1 228,803 - +0.2 228,809 + +1.3 228,814 - +0.3 228,815 + -1.3 228,817 - -0.1 228,846 - +0.3 228,875 + +0.0 229,311 + -0.7 229,319 - +0.4 229,329 + +0.3 229,337 - -0.1 229,370 + -1.3 229,378 - -0.5 229,378 - -1.8 229,378 - +0.5 229,380 + -0.8 229,380 + +1.3 229,383 + +0.1 229,385 + +1.4 229,385 + -1.2 229,388 + -0.5 229,388 - -0.8 229,390 + +0.5 229,391 - -0.5 229,395 + -0.0 229,398 - +0.0 229,403 - -1.8 229,475 + GFF224 0.34 -0.4 229,477 + GFF224 0.35 -1.3 229,477 + GFF224 0.35 -0.6 229,477 + GFF224 0.35 +0.7 229,485 - GFF224 0.38 +0.1 229,485 - GFF224 0.38 +1.4 229,513 + GFF224 0.51 +1.7 229,520 - GFF224 0.54 +0.3 229,540 - GFF224 0.63 -1.5 229,565 - GFF224 0.74 +0.8 229,566 - GFF224 0.75 -1.7 229,566 - GFF224 0.75 +0.7 229,591 + GFF224 0.86 -0.4 229,592 + GFF224 0.86 -0.2 229,615 + -0.1 229,618 - +0.3 229,623 - -1.0 229,623 - -2.0 229,679 + -0.5 229,679 + -2.7 229,679 + +0.2 229,681 + -0.5 229,681 + -0.2 229,681 + -0.1 229,687 - +0.7 229,689 - -0.1 229,689 - +0.6 229,693 + +0.7 229,693 + +0.5 229,694 + +0.5 229,694 + -1.5 229,694 + -0.3 229,695 - -0.1 229,695 - -1.2 229,700 + -0.0 229,701 - +0.3 229,702 - -0.1
Or see this region's nucleotide sequence