Strain Fitness in Sphingobium sp. HT1-2 around GFF66

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF62 and GFF63 are separated by 105 nucleotidesGFF63 and GFF64 are separated by 93 nucleotidesGFF64 and GFF65 are separated by 120 nucleotidesGFF65 and GFF66 are separated by 72 nucleotidesGFF66 and GFF67 are separated by 203 nucleotides GFF62 - tRNA-Arg-ACG, at 53,797 to 53,873 GFF62 GFF63 - hypothetical protein, at 53,979 to 54,158 GFF63 GFF64 - hypothetical protein, at 54,252 to 54,434 GFF64 GFF65 - tRNA-Arg-ACG, at 54,555 to 54,631 GFF65 GFF66 - Cobyric acid synthase (EC 6.3.5.10), at 54,704 to 56,152 GFF66 GFF67 - hypothetical protein, at 56,356 to 57,999 GFF67 Position (kb) 54 55 56 57Strain fitness (log2 ratio) -2 -1 0 1 2 3at 53.712 kb on + strandat 53.812 kb on - strand, within GFF62at 53.907 kb on + strandat 53.981 kb on + strandat 53.988 kb on + strandat 53.988 kb on + strandat 53.988 kb on + strandat 53.996 kb on - strandat 54.010 kb on + strand, within GFF63at 54.035 kb on + strand, within GFF63at 54.043 kb on - strand, within GFF63at 54.044 kb on - strand, within GFF63at 54.075 kb on + strand, within GFF63at 54.092 kb on + strand, within GFF63at 54.105 kb on + strand, within GFF63at 54.105 kb on + strand, within GFF63at 54.113 kb on - strand, within GFF63at 54.113 kb on - strand, within GFF63at 54.119 kb on + strand, within GFF63at 54.127 kb on - strand, within GFF63at 54.159 kb on + strandat 54.198 kb on + strandat 54.259 kb on + strandat 54.262 kb on - strandat 54.301 kb on - strand, within GFF64at 54.337 kb on - strand, within GFF64at 54.369 kb on + strand, within GFF64at 54.428 kb on + strandat 54.548 kb on + strandat 54.794 kb on + strandat 54.802 kb on - strandat 54.903 kb on + strand, within GFF66at 55.020 kb on + strand, within GFF66at 55.020 kb on + strand, within GFF66at 55.038 kb on + strand, within GFF66at 55.038 kb on - strand, within GFF66at 55.073 kb on - strand, within GFF66at 55.073 kb on - strand, within GFF66at 55.075 kb on + strand, within GFF66at 55.083 kb on - strand, within GFF66at 55.083 kb on - strand, within GFF66at 55.083 kb on - strand, within GFF66at 55.097 kb on + strand, within GFF66at 55.199 kb on + strand, within GFF66at 55.209 kb on + strand, within GFF66at 55.209 kb on + strand, within GFF66at 55.209 kb on + strand, within GFF66at 55.209 kb on + strand, within GFF66at 55.217 kb on - strand, within GFF66at 55.230 kb on + strand, within GFF66at 55.252 kb on + strand, within GFF66at 55.260 kb on - strand, within GFF66at 55.289 kb on - strand, within GFF66at 55.321 kb on + strand, within GFF66at 55.327 kb on + strand, within GFF66at 55.327 kb on + strand, within GFF66at 55.346 kb on - strand, within GFF66at 55.348 kb on + strand, within GFF66at 55.376 kb on + strand, within GFF66at 55.389 kb on - strand, within GFF66at 55.441 kb on - strand, within GFF66at 55.457 kb on - strand, within GFF66at 55.489 kb on + strand, within GFF66at 55.497 kb on - strand, within GFF66at 55.497 kb on - strand, within GFF66at 55.554 kb on - strand, within GFF66at 55.600 kb on + strand, within GFF66at 55.652 kb on - strand, within GFF66at 55.683 kb on - strand, within GFF66at 55.740 kb on - strand, within GFF66at 55.740 kb on - strand, within GFF66at 55.785 kb on + strand, within GFF66at 55.803 kb on - strand, within GFF66at 55.815 kb on + strand, within GFF66at 55.916 kb on - strand, within GFF66at 55.916 kb on - strand, within GFF66at 56.040 kb on + strandat 56.066 kb on - strandat 56.080 kb on - strandat 56.153 kb on + strandat 56.166 kb on - strandat 56.180 kb on + strandat 56.188 kb on - strandat 56.197 kb on - strandat 56.263 kb on - strandat 56.368 kb on + strandat 56.413 kb on + strandat 56.419 kb on - strandat 56.419 kb on - strandat 56.421 kb on - strandat 56.421 kb on - strandat 56.421 kb on - strandat 56.421 kb on - strandat 56.430 kb on + strandat 56.438 kb on - strandat 56.443 kb on - strandat 56.446 kb on + strandat 56.474 kb on + strandat 56.474 kb on + strandat 56.475 kb on - strandat 56.482 kb on - strandat 56.482 kb on - strandat 56.482 kb on - strandat 56.482 kb on - strandat 56.531 kb on - strand, within GFF67at 56.538 kb on + strand, within GFF67at 56.543 kb on + strand, within GFF67at 56.544 kb on - strand, within GFF67at 56.557 kb on - strand, within GFF67at 56.568 kb on + strand, within GFF67at 56.568 kb on + strand, within GFF67at 56.568 kb on + strand, within GFF67at 56.576 kb on - strand, within GFF67at 56.576 kb on - strand, within GFF67at 56.582 kb on + strand, within GFF67at 56.590 kb on - strand, within GFF67at 56.592 kb on + strand, within GFF67at 56.592 kb on + strand, within GFF67at 56.608 kb on - strand, within GFF67at 56.609 kb on + strand, within GFF67at 56.609 kb on + strand, within GFF67at 56.614 kb on + strand, within GFF67at 56.647 kb on - strand, within GFF67at 56.666 kb on + strand, within GFF67at 56.690 kb on - strand, within GFF67at 56.693 kb on + strand, within GFF67at 56.693 kb on + strand, within GFF67at 56.700 kb on - strand, within GFF67at 56.700 kb on - strand, within GFF67at 56.700 kb on - strand, within GFF67at 56.700 kb on - strand, within GFF67at 56.701 kb on - strand, within GFF67at 56.701 kb on - strand, within GFF67at 56.812 kb on - strand, within GFF67at 56.830 kb on + strand, within GFF67at 56.838 kb on - strand, within GFF67at 56.845 kb on + strand, within GFF67at 56.845 kb on - strand, within GFF67at 56.898 kb on + strand, within GFF67at 56.906 kb on - strand, within GFF67at 56.940 kb on + strand, within GFF67at 56.967 kb on - strand, within GFF67at 56.974 kb on + strand, within GFF67at 56.974 kb on + strand, within GFF67at 56.974 kb on + strand, within GFF67at 56.977 kb on - strand, within GFF67at 56.977 kb on - strand, within GFF67at 56.977 kb on - strand, within GFF67at 56.977 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.982 kb on - strand, within GFF67at 56.989 kb on - strand, within GFF67at 57.019 kb on + strand, within GFF67at 57.019 kb on + strand, within GFF67at 57.019 kb on + strand, within GFF67at 57.019 kb on + strand, within GFF67at 57.019 kb on + strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.027 kb on - strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.029 kb on + strand, within GFF67at 57.034 kb on + strand, within GFF67at 57.034 kb on + strand, within GFF67at 57.037 kb on - strand, within GFF67at 57.037 kb on - strand, within GFF67at 57.037 kb on - strand, within GFF67at 57.037 kb on - strand, within GFF67at 57.037 kb on - strand, within GFF67at 57.039 kb on + strand, within GFF67at 57.039 kb on + strand, within GFF67at 57.039 kb on + strand, within GFF67at 57.042 kb on - strand, within GFF67at 57.044 kb on + strand, within GFF67at 57.068 kb on + strand, within GFF67at 57.073 kb on + strand, within GFF67at 57.073 kb on + strand, within GFF67at 57.073 kb on + strand, within GFF67at 57.073 kb on + strand, within GFF67at 57.078 kb on + strand, within GFF67at 57.081 kb on - strand, within GFF67at 57.081 kb on - strand, within GFF67at 57.101 kb on - strand, within GFF67at 57.130 kb on + strand, within GFF67at 57.130 kb on + strand, within GFF67at 57.130 kb on + strand, within GFF67at 57.130 kb on + strand, within GFF67at 57.138 kb on - strand, within GFF67at 57.138 kb on - strand, within GFF67at 57.138 kb on - strand, within GFF67

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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53,712 + -0.4
53,812 - GFF62 0.19 +1.5
53,907 + -1.5
53,981 + +1.0
53,988 + -0.6
53,988 + +0.8
53,988 + -2.0
53,996 - +0.3
54,010 + GFF63 0.17 -0.4
54,035 + GFF63 0.31 +0.1
54,043 - GFF63 0.36 -0.1
54,044 - GFF63 0.36 +0.2
54,075 + GFF63 0.53 +0.6
54,092 + GFF63 0.63 -0.1
54,105 + GFF63 0.70 +2.2
54,105 + GFF63 0.70 -0.0
54,113 - GFF63 0.74 +0.1
54,113 - GFF63 0.74 -0.3
54,119 + GFF63 0.78 +0.9
54,127 - GFF63 0.82 -1.1
54,159 + +0.1
54,198 + +0.0
54,259 + -1.6
54,262 - -0.9
54,301 - GFF64 0.27 -1.1
54,337 - GFF64 0.46 +0.0
54,369 + GFF64 0.64 -1.0
54,428 + -0.5
54,548 + -0.0
54,794 + -2.6
54,802 - -0.6
54,903 + GFF66 0.14 +1.1
55,020 + GFF66 0.22 -1.4
55,020 + GFF66 0.22 -0.1
55,038 + GFF66 0.23 -1.3
55,038 - GFF66 0.23 -0.1
55,073 - GFF66 0.25 -0.0
55,073 - GFF66 0.25 -2.1
55,075 + GFF66 0.26 -0.9
55,083 - GFF66 0.26 -0.6
55,083 - GFF66 0.26 -1.4
55,083 - GFF66 0.26 -2.1
55,097 + GFF66 0.27 -0.7
55,199 + GFF66 0.34 -1.6
55,209 + GFF66 0.35 +0.4
55,209 + GFF66 0.35 -1.7
55,209 + GFF66 0.35 -0.1
55,209 + GFF66 0.35 -1.0
55,217 - GFF66 0.35 -1.7
55,230 + GFF66 0.36 -1.0
55,252 + GFF66 0.38 -2.4
55,260 - GFF66 0.38 -1.3
55,289 - GFF66 0.40 -1.6
55,321 + GFF66 0.43 -1.6
55,327 + GFF66 0.43 +0.6
55,327 + GFF66 0.43 -0.7
55,346 - GFF66 0.44 -1.1
55,348 + GFF66 0.44 +0.6
55,376 + GFF66 0.46 -0.1
55,389 - GFF66 0.47 -0.5
55,441 - GFF66 0.51 +0.1
55,457 - GFF66 0.52 -0.6
55,489 + GFF66 0.54 -2.6
55,497 - GFF66 0.55 -0.4
55,497 - GFF66 0.55 -0.4
55,554 - GFF66 0.59 -0.7
55,600 + GFF66 0.62 -0.1
55,652 - GFF66 0.65 -1.9
55,683 - GFF66 0.68 -0.1
55,740 - GFF66 0.71 -0.1
55,740 - GFF66 0.71 -2.5
55,785 + GFF66 0.75 +0.1
55,803 - GFF66 0.76 -0.1
55,815 + GFF66 0.77 -0.9
55,916 - GFF66 0.84 -2.4
55,916 - GFF66 0.84 -1.0
56,040 + -2.5
56,066 - -0.6
56,080 - -2.0
56,153 + +2.0
56,166 - -0.6
56,180 + -0.7
56,188 - +0.6
56,197 - +0.6
56,263 - -1.6
56,368 + +1.0
56,413 + +0.2
56,419 - +0.4
56,419 - -0.5
56,421 - -2.1
56,421 - +0.8
56,421 - -0.4
56,421 - -0.3
56,430 + -0.6
56,438 - +0.9
56,443 - +0.0
56,446 + -1.9
56,474 + -2.2
56,474 + +0.2
56,475 - +0.1
56,482 - -0.2
56,482 - -1.1
56,482 - -0.6
56,482 - +0.4
56,531 - GFF67 0.11 +1.0
56,538 + GFF67 0.11 -0.7
56,543 + GFF67 0.11 -1.3
56,544 - GFF67 0.11 -0.9
56,557 - GFF67 0.12 -1.4
56,568 + GFF67 0.13 +0.0
56,568 + GFF67 0.13 -0.5
56,568 + GFF67 0.13 +0.2
56,576 - GFF67 0.13 +0.4
56,576 - GFF67 0.13 +0.2
56,582 + GFF67 0.14 +1.1
56,590 - GFF67 0.14 +1.0
56,592 + GFF67 0.14 +0.6
56,592 + GFF67 0.14 -0.8
56,608 - GFF67 0.15 +0.8
56,609 + GFF67 0.15 +1.2
56,609 + GFF67 0.15 -1.3
56,614 + GFF67 0.16 -0.1
56,647 - GFF67 0.18 +0.9
56,666 + GFF67 0.19 -0.1
56,690 - GFF67 0.20 +0.6
56,693 + GFF67 0.20 -2.2
56,693 + GFF67 0.20 +0.3
56,700 - GFF67 0.21 -0.6
56,700 - GFF67 0.21 +0.7
56,700 - GFF67 0.21 -1.1
56,700 - GFF67 0.21 -0.1
56,701 - GFF67 0.21 +0.2
56,701 - GFF67 0.21 +0.6
56,812 - GFF67 0.28 +0.3
56,830 + GFF67 0.29 -0.2
56,838 - GFF67 0.29 -1.3
56,845 + GFF67 0.30 +0.6
56,845 - GFF67 0.30 +0.2
56,898 + GFF67 0.33 -0.9
56,906 - GFF67 0.33 +0.8
56,940 + GFF67 0.36 +0.2
56,967 - GFF67 0.37 -0.5
56,974 + GFF67 0.38 +0.2
56,974 + GFF67 0.38 +3.3
56,974 + GFF67 0.38 -1.1
56,977 - GFF67 0.38 +1.0
56,977 - GFF67 0.38 -1.0
56,977 - GFF67 0.38 -2.1
56,977 - GFF67 0.38 +1.4
56,982 - GFF67 0.38 +0.1
56,982 - GFF67 0.38 +1.0
56,982 - GFF67 0.38 -0.5
56,982 - GFF67 0.38 -0.4
56,982 - GFF67 0.38 +0.7
56,982 - GFF67 0.38 +0.9
56,982 - GFF67 0.38 -0.7
56,982 - GFF67 0.38 +0.2
56,989 - GFF67 0.39 +0.4
57,019 + GFF67 0.40 -0.9
57,019 + GFF67 0.40 +0.6
57,019 + GFF67 0.40 -0.6
57,019 + GFF67 0.40 -0.7
57,019 + GFF67 0.40 -0.8
57,027 - GFF67 0.41 +1.3
57,027 - GFF67 0.41 -0.2
57,027 - GFF67 0.41 +0.1
57,027 - GFF67 0.41 +0.5
57,027 - GFF67 0.41 -0.6
57,027 - GFF67 0.41 +1.3
57,027 - GFF67 0.41 -0.2
57,029 + GFF67 0.41 +0.8
57,029 + GFF67 0.41 +1.1
57,029 + GFF67 0.41 -0.6
57,029 + GFF67 0.41 -0.6
57,029 + GFF67 0.41 +0.3
57,029 + GFF67 0.41 +1.2
57,029 + GFF67 0.41 +0.4
57,029 + GFF67 0.41 +0.2
57,029 + GFF67 0.41 -1.3
57,029 + GFF67 0.41 +0.1
57,029 + GFF67 0.41 -0.6
57,029 + GFF67 0.41 -0.4
57,034 + GFF67 0.41 -0.3
57,034 + GFF67 0.41 +0.8
57,037 - GFF67 0.41 +0.4
57,037 - GFF67 0.41 -0.0
57,037 - GFF67 0.41 +0.3
57,037 - GFF67 0.41 -0.5
57,037 - GFF67 0.41 -0.8
57,039 + GFF67 0.42 +0.1
57,039 + GFF67 0.42 +0.5
57,039 + GFF67 0.42 -1.3
57,042 - GFF67 0.42 -0.4
57,044 + GFF67 0.42 +0.3
57,068 + GFF67 0.43 +2.4
57,073 + GFF67 0.44 +1.2
57,073 + GFF67 0.44 +1.7
57,073 + GFF67 0.44 +0.8
57,073 + GFF67 0.44 +0.5
57,078 + GFF67 0.44 +0.4
57,081 - GFF67 0.44 -0.7
57,081 - GFF67 0.44 +0.7
57,101 - GFF67 0.45 -0.7
57,130 + GFF67 0.47 -0.4
57,130 + GFF67 0.47 -0.2
57,130 + GFF67 0.47 -0.4
57,130 + GFF67 0.47 +0.1
57,138 - GFF67 0.48 +0.9
57,138 - GFF67 0.48 -0.7
57,138 - GFF67 0.48 +0.1

Or see this region's nucleotide sequence