Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF356 and GFF357 are separated by 131 nucleotides GFF357 and GFF358 are separated by 83 nucleotides GFF358 and GFF359 are separated by 12 nucleotides
GFF356 - Sulfur carrier protein ThiS / Thiazole synthase (EC 2.8.1.10), at 348,163 to 349,164
GFF356
GFF357 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 349,296 to 349,736
GFF357
GFF358 - Biotin carboxyl carrier protein of acetyl-CoA carboxylase, at 349,820 to 350,308
GFF358
GFF359 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 350,321 to 351,673
GFF359
Position (kb)
349
350
351 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 348.835 kb on - strand, within GFF356 at 348.840 kb on - strand, within GFF356 at 348.850 kb on - strand, within GFF356 at 348.852 kb on + strand, within GFF356 at 348.885 kb on - strand, within GFF356 at 348.894 kb on + strand, within GFF356 at 348.901 kb on + strand, within GFF356 at 348.909 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.910 kb on - strand, within GFF356 at 348.914 kb on + strand, within GFF356 at 348.920 kb on - strand, within GFF356 at 348.926 kb on + strand, within GFF356 at 348.933 kb on - strand, within GFF356 at 348.946 kb on - strand, within GFF356 at 348.953 kb on - strand, within GFF356 at 348.971 kb on + strand, within GFF356 at 348.979 kb on - strand, within GFF356 at 348.979 kb on - strand, within GFF356 at 348.988 kb on + strand, within GFF356 at 348.988 kb on + strand, within GFF356 at 348.996 kb on - strand, within GFF356 at 348.997 kb on - strand, within GFF356 at 349.006 kb on + strand, within GFF356 at 349.006 kb on + strand, within GFF356 at 349.006 kb on + strand, within GFF356 at 349.011 kb on + strand, within GFF356 at 349.014 kb on - strand, within GFF356 at 349.014 kb on - strand, within GFF356 at 349.019 kb on - strand, within GFF356 at 349.021 kb on + strand, within GFF356 at 349.021 kb on + strand, within GFF356 at 349.021 kb on - strand, within GFF356 at 349.037 kb on - strand, within GFF356 at 349.039 kb on + strand, within GFF356 at 349.046 kb on + strand, within GFF356 at 349.077 kb on - strand at 349.077 kb on - strand at 349.091 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.095 kb on + strand at 349.096 kb on + strand at 349.096 kb on + strand at 349.096 kb on + strand at 349.096 kb on + strand at 349.096 kb on + strand at 349.096 kb on + strand at 349.098 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.103 kb on - strand at 349.104 kb on - strand at 349.104 kb on - strand at 349.113 kb on - strand at 349.135 kb on - strand at 349.146 kb on - strand at 349.166 kb on + strand at 349.166 kb on + strand at 349.166 kb on + strand at 349.166 kb on + strand at 349.174 kb on - strand at 349.174 kb on - strand at 349.174 kb on - strand at 349.217 kb on + strand at 349.217 kb on + strand at 349.218 kb on + strand at 349.225 kb on - strand at 349.225 kb on - strand at 349.225 kb on - strand at 349.241 kb on - strand at 349.270 kb on - strand at 349.270 kb on - strand at 349.271 kb on + strand at 349.271 kb on + strand at 349.271 kb on + strand at 349.275 kb on - strand at 349.275 kb on - strand at 349.279 kb on - strand at 349.279 kb on - strand at 349.279 kb on - strand at 349.338 kb on - strand at 349.608 kb on - strand, within GFF357 at 349.678 kb on - strand, within GFF357 at 349.722 kb on + strand at 349.730 kb on - strand at 349.730 kb on - strand at 349.730 kb on - strand at 349.731 kb on + strand at 349.731 kb on + strand at 349.731 kb on - strand at 349.733 kb on + strand at 349.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 348,835 - GFF356 0.67 +0.3 348,840 - GFF356 0.68 +0.1 348,850 - GFF356 0.69 -1.9 348,852 + GFF356 0.69 -1.7 348,885 - GFF356 0.72 -1.2 348,894 + GFF356 0.73 +0.2 348,901 + GFF356 0.74 +0.6 348,909 - GFF356 0.74 -0.5 348,910 - GFF356 0.75 +1.0 348,910 - GFF356 0.75 +0.8 348,910 - GFF356 0.75 +0.6 348,910 - GFF356 0.75 +1.4 348,910 - GFF356 0.75 -1.1 348,910 - GFF356 0.75 -1.1 348,914 + GFF356 0.75 -0.2 348,920 - GFF356 0.76 +2.2 348,926 + GFF356 0.76 +0.2 348,933 - GFF356 0.77 -0.2 348,946 - GFF356 0.78 -1.2 348,953 - GFF356 0.79 +0.1 348,971 + GFF356 0.81 -0.1 348,979 - GFF356 0.81 -1.0 348,979 - GFF356 0.81 -0.4 348,988 + GFF356 0.82 -0.9 348,988 + GFF356 0.82 +0.1 348,996 - GFF356 0.83 +0.3 348,997 - GFF356 0.83 +0.1 349,006 + GFF356 0.84 +1.0 349,006 + GFF356 0.84 +0.2 349,006 + GFF356 0.84 +0.1 349,011 + GFF356 0.85 -0.3 349,014 - GFF356 0.85 -0.9 349,014 - GFF356 0.85 -0.5 349,019 - GFF356 0.85 -1.3 349,021 + GFF356 0.86 +0.4 349,021 + GFF356 0.86 +0.4 349,021 - GFF356 0.86 -0.1 349,037 - GFF356 0.87 +0.1 349,039 + GFF356 0.87 +0.2 349,046 + GFF356 0.88 -1.6 349,077 - +0.4 349,077 - +1.0 349,091 + -0.3 349,095 + -0.1 349,095 + -0.3 349,095 + +0.8 349,095 + +0.1 349,095 + +0.7 349,095 + -1.8 349,095 + +0.5 349,096 + -1.4 349,096 + -1.2 349,096 + -0.5 349,096 + -0.7 349,096 + +0.1 349,096 + +0.4 349,098 - +0.9 349,103 - +0.9 349,103 - +1.0 349,103 - -1.2 349,103 - -1.5 349,103 - -1.1 349,103 - -1.1 349,103 - -1.1 349,104 - +2.2 349,104 - +0.1 349,113 - -0.3 349,135 - -0.3 349,146 - -1.9 349,166 + -1.0 349,166 + +0.7 349,166 + -1.9 349,166 + +0.5 349,174 - -1.8 349,174 - -1.9 349,174 - +1.2 349,217 + -0.9 349,217 + +0.2 349,218 + +0.3 349,225 - -0.5 349,225 - +0.1 349,225 - -1.1 349,241 - -1.1 349,270 - -0.9 349,270 - -0.2 349,271 + -1.6 349,271 + -0.8 349,271 + +0.8 349,275 - +0.1 349,275 - -1.3 349,279 - -0.1 349,279 - -0.2 349,279 - -1.5 349,338 - -0.2 349,608 - GFF357 0.71 -0.7 349,678 - GFF357 0.87 -1.1 349,722 + +0.4 349,730 - -1.2 349,730 - +0.2 349,730 - +0.1 349,731 + -0.0 349,731 + +0.4 349,731 - -1.1 349,733 + +1.2 349,748 - -0.5
Or see this region's nucleotide sequence