Strain Fitness in Sphingobium sp. HT1-2 around GFF2542

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2541 and GFF2542 are separated by 278 nucleotidesGFF2542 and GFF2543 are separated by 7 nucleotidesGFF2543 and GFF2544 are separated by 143 nucleotides GFF2541 - hypothetical protein, at 2,558,468 to 2,559,361 GFF2541 GFF2542 - Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase, at 2,559,640 to 2,560,605 GFF2542 GFF2543 - hypothetical protein, at 2,560,613 to 2,561,101 GFF2543 GFF2544 - Lipopolysaccharide export system permease protein LptF, at 2,561,245 to 2,562,456 GFF2544 Position (kb) 2559 2560 2561Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2558.642 kb on + strand, within GFF2541at 2558.642 kb on + strand, within GFF2541at 2558.642 kb on + strand, within GFF2541at 2558.650 kb on - strand, within GFF2541at 2558.713 kb on + strand, within GFF2541at 2558.722 kb on + strand, within GFF2541at 2558.744 kb on - strand, within GFF2541at 2558.756 kb on + strand, within GFF2541at 2558.764 kb on - strand, within GFF2541at 2558.770 kb on - strand, within GFF2541at 2558.782 kb on - strand, within GFF2541at 2558.783 kb on - strand, within GFF2541at 2558.807 kb on + strand, within GFF2541at 2558.815 kb on - strand, within GFF2541at 2558.828 kb on - strand, within GFF2541at 2558.885 kb on + strand, within GFF2541at 2558.885 kb on + strand, within GFF2541at 2558.885 kb on + strand, within GFF2541at 2558.918 kb on + strand, within GFF2541at 2558.926 kb on - strand, within GFF2541at 2558.926 kb on - strand, within GFF2541at 2558.929 kb on + strand, within GFF2541at 2558.936 kb on - strand, within GFF2541at 2558.937 kb on - strand, within GFF2541at 2558.948 kb on - strand, within GFF2541at 2558.962 kb on + strand, within GFF2541at 2558.962 kb on + strand, within GFF2541at 2558.962 kb on + strand, within GFF2541at 2558.970 kb on - strand, within GFF2541at 2559.041 kb on - strand, within GFF2541at 2559.069 kb on + strand, within GFF2541at 2559.073 kb on + strand, within GFF2541at 2559.079 kb on - strand, within GFF2541at 2559.084 kb on + strand, within GFF2541at 2559.092 kb on - strand, within GFF2541at 2559.092 kb on - strand, within GFF2541at 2559.094 kb on + strand, within GFF2541at 2559.158 kb on - strand, within GFF2541at 2559.168 kb on + strand, within GFF2541at 2559.235 kb on - strand, within GFF2541at 2559.249 kb on - strand, within GFF2541at 2559.249 kb on - strand, within GFF2541at 2559.271 kb on - strand, within GFF2541at 2559.383 kb on - strandat 2559.387 kb on + strandat 2559.438 kb on - strandat 2559.447 kb on - strandat 2559.550 kb on + strandat 2559.609 kb on + strandat 2559.747 kb on - strand, within GFF2542at 2559.749 kb on + strand, within GFF2542at 2559.782 kb on - strand, within GFF2542at 2559.834 kb on - strand, within GFF2542at 2559.834 kb on - strand, within GFF2542at 2560.146 kb on + strand, within GFF2542at 2560.164 kb on - strand, within GFF2542at 2560.171 kb on - strand, within GFF2542at 2560.244 kb on + strand, within GFF2542at 2560.306 kb on - strand, within GFF2542at 2560.336 kb on - strand, within GFF2542at 2560.573 kb on - strandat 2560.651 kb on - strandat 2560.654 kb on + strandat 2560.654 kb on + strandat 2560.654 kb on + strandat 2560.662 kb on - strand, within GFF2543at 2560.667 kb on - strand, within GFF2543at 2560.673 kb on + strand, within GFF2543at 2560.680 kb on + strand, within GFF2543at 2560.686 kb on + strand, within GFF2543at 2560.759 kb on + strand, within GFF2543at 2560.846 kb on - strand, within GFF2543at 2560.855 kb on + strand, within GFF2543at 2560.860 kb on + strand, within GFF2543at 2560.860 kb on + strand, within GFF2543at 2560.868 kb on - strand, within GFF2543at 2560.892 kb on - strand, within GFF2543at 2560.956 kb on - strand, within GFF2543at 2560.956 kb on - strand, within GFF2543at 2560.963 kb on - strand, within GFF2543at 2560.967 kb on + strand, within GFF2543at 2560.975 kb on - strand, within GFF2543at 2561.001 kb on + strand, within GFF2543at 2561.006 kb on - strand, within GFF2543at 2561.036 kb on + strand, within GFF2543at 2561.044 kb on - strand, within GFF2543at 2561.052 kb on + strand, within GFF2543at 2561.099 kb on - strandat 2561.122 kb on - strandat 2561.129 kb on - strandat 2561.240 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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2,558,642 + GFF2541 0.19 -1.3
2,558,642 + GFF2541 0.19 -0.3
2,558,642 + GFF2541 0.19 -1.1
2,558,650 - GFF2541 0.20 -1.8
2,558,713 + GFF2541 0.27 -0.3
2,558,722 + GFF2541 0.28 -0.7
2,558,744 - GFF2541 0.31 +1.2
2,558,756 + GFF2541 0.32 +0.6
2,558,764 - GFF2541 0.33 +0.9
2,558,770 - GFF2541 0.34 -0.0
2,558,782 - GFF2541 0.35 -1.8
2,558,783 - GFF2541 0.35 +0.5
2,558,807 + GFF2541 0.38 -0.5
2,558,815 - GFF2541 0.39 +0.5
2,558,828 - GFF2541 0.40 +0.9
2,558,885 + GFF2541 0.47 +0.0
2,558,885 + GFF2541 0.47 +0.2
2,558,885 + GFF2541 0.47 +0.0
2,558,918 + GFF2541 0.50 -0.7
2,558,926 - GFF2541 0.51 +0.0
2,558,926 - GFF2541 0.51 -0.0
2,558,929 + GFF2541 0.52 +0.7
2,558,936 - GFF2541 0.52 +1.7
2,558,937 - GFF2541 0.52 -1.4
2,558,948 - GFF2541 0.54 +1.2
2,558,962 + GFF2541 0.55 +0.9
2,558,962 + GFF2541 0.55 -0.7
2,558,962 + GFF2541 0.55 -0.1
2,558,970 - GFF2541 0.56 +0.1
2,559,041 - GFF2541 0.64 -0.6
2,559,069 + GFF2541 0.67 -1.6
2,559,073 + GFF2541 0.68 +0.3
2,559,079 - GFF2541 0.68 +0.0
2,559,084 + GFF2541 0.69 -2.2
2,559,092 - GFF2541 0.70 -1.2
2,559,092 - GFF2541 0.70 -3.6
2,559,094 + GFF2541 0.70 -0.9
2,559,158 - GFF2541 0.77 -0.2
2,559,168 + GFF2541 0.78 -0.5
2,559,235 - GFF2541 0.86 +0.6
2,559,249 - GFF2541 0.87 -0.8
2,559,249 - GFF2541 0.87 +1.2
2,559,271 - GFF2541 0.90 -0.2
2,559,383 - -0.6
2,559,387 + -0.9
2,559,438 - -0.8
2,559,447 - -0.9
2,559,550 + -1.5
2,559,609 + -1.3
2,559,747 - GFF2542 0.11 -0.9
2,559,749 + GFF2542 0.11 -0.1
2,559,782 - GFF2542 0.15 +0.1
2,559,834 - GFF2542 0.20 +1.8
2,559,834 - GFF2542 0.20 +0.5
2,560,146 + GFF2542 0.52 -0.5
2,560,164 - GFF2542 0.54 +1.7
2,560,171 - GFF2542 0.55 -0.1
2,560,244 + GFF2542 0.63 +0.5
2,560,306 - GFF2542 0.69 -0.3
2,560,336 - GFF2542 0.72 +1.5
2,560,573 - +0.9
2,560,651 - +0.3
2,560,654 + -0.7
2,560,654 + -0.7
2,560,654 + -1.6
2,560,662 - GFF2543 0.10 -2.3
2,560,667 - GFF2543 0.11 -1.4
2,560,673 + GFF2543 0.12 -0.8
2,560,680 + GFF2543 0.14 -0.2
2,560,686 + GFF2543 0.15 +0.7
2,560,759 + GFF2543 0.30 +1.3
2,560,846 - GFF2543 0.48 +0.4
2,560,855 + GFF2543 0.49 -1.6
2,560,860 + GFF2543 0.51 -0.3
2,560,860 + GFF2543 0.51 +0.9
2,560,868 - GFF2543 0.52 +1.5
2,560,892 - GFF2543 0.57 +1.2
2,560,956 - GFF2543 0.70 -0.6
2,560,956 - GFF2543 0.70 -0.9
2,560,963 - GFF2543 0.72 -0.2
2,560,967 + GFF2543 0.72 -1.0
2,560,975 - GFF2543 0.74 -0.6
2,561,001 + GFF2543 0.79 -1.1
2,561,006 - GFF2543 0.80 +0.3
2,561,036 + GFF2543 0.87 +0.7
2,561,044 - GFF2543 0.88 -0.7
2,561,052 + GFF2543 0.90 -2.5
2,561,099 - +0.5
2,561,122 - +0.7
2,561,129 - -0.1
2,561,240 + +0.1

Or see this region's nucleotide sequence