Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1954 and GFF1955 overlap by 4 nucleotides GFF1955 and GFF1956 overlap by 4 nucleotides GFF1956 and GFF1957 are separated by 183 nucleotides
GFF1954 - Cell division protein FtsL, at 1,981,468 to 1,982,121
GFF1954
GFF1955 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase (EC 2.1.1.199), at 1,982,118 to 1,983,065
GFF1955
GFF1956 - Transcriptional regulator MraZ, at 1,983,062 to 1,983,559
GFF1956
GFF1957 - hypothetical protein, at 1,983,743 to 1,984,966
GFF1957
Position (kb)
1983
1984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1982.178 kb on - strand at 1982.178 kb on - strand at 1982.359 kb on - strand, within GFF1955 at 1983.096 kb on - strand at 1983.096 kb on - strand at 1983.096 kb on - strand at 1983.371 kb on - strand, within GFF1956 at 1983.583 kb on - strand at 1983.767 kb on - strand at 1983.823 kb on + strand at 1983.830 kb on + strand at 1983.831 kb on - strand at 1983.831 kb on - strand at 1983.839 kb on + strand at 1983.839 kb on + strand at 1983.847 kb on - strand at 1983.847 kb on - strand at 1983.849 kb on + strand at 1983.857 kb on - strand at 1983.882 kb on - strand, within GFF1957 at 1983.939 kb on - strand, within GFF1957 at 1983.969 kb on + strand, within GFF1957 at 1983.982 kb on + strand, within GFF1957 at 1984.013 kb on - strand, within GFF1957 at 1984.025 kb on + strand, within GFF1957 at 1984.030 kb on + strand, within GFF1957 at 1984.030 kb on + strand, within GFF1957 at 1984.033 kb on - strand, within GFF1957 at 1984.038 kb on - strand, within GFF1957 at 1984.068 kb on + strand, within GFF1957 at 1984.084 kb on + strand, within GFF1957 at 1984.084 kb on + strand, within GFF1957 at 1984.084 kb on + strand, within GFF1957 at 1984.087 kb on - strand, within GFF1957 at 1984.089 kb on + strand, within GFF1957 at 1984.092 kb on - strand, within GFF1957 at 1984.097 kb on - strand, within GFF1957 at 1984.110 kb on + strand, within GFF1957 at 1984.134 kb on + strand, within GFF1957 at 1984.190 kb on + strand, within GFF1957 at 1984.190 kb on + strand, within GFF1957 at 1984.191 kb on - strand, within GFF1957 at 1984.193 kb on - strand, within GFF1957 at 1984.193 kb on - strand, within GFF1957 at 1984.193 kb on - strand, within GFF1957 at 1984.193 kb on - strand, within GFF1957 at 1984.193 kb on - strand, within GFF1957 at 1984.198 kb on - strand, within GFF1957 at 1984.198 kb on - strand, within GFF1957 at 1984.208 kb on - strand, within GFF1957 at 1984.225 kb on + strand, within GFF1957 at 1984.280 kb on + strand, within GFF1957 at 1984.338 kb on + strand, within GFF1957 at 1984.370 kb on + strand, within GFF1957 at 1984.370 kb on + strand, within GFF1957 at 1984.373 kb on - strand, within GFF1957 at 1984.400 kb on + strand, within GFF1957 at 1984.438 kb on + strand, within GFF1957 at 1984.446 kb on - strand, within GFF1957 at 1984.480 kb on + strand, within GFF1957 at 1984.482 kb on - strand, within GFF1957 at 1984.485 kb on + strand, within GFF1957 at 1984.488 kb on - strand, within GFF1957 at 1984.491 kb on + strand, within GFF1957 at 1984.491 kb on + strand, within GFF1957 at 1984.493 kb on - strand, within GFF1957 at 1984.493 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.499 kb on - strand, within GFF1957 at 1984.501 kb on + strand, within GFF1957 at 1984.503 kb on + strand, within GFF1957 at 1984.503 kb on + strand, within GFF1957 at 1984.503 kb on + strand, within GFF1957 at 1984.511 kb on - strand, within GFF1957 at 1984.511 kb on - strand, within GFF1957 at 1984.519 kb on + strand, within GFF1957 at 1984.537 kb on + strand, within GFF1957
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,982,178 - +1.5 1,982,178 - -0.4 1,982,359 - GFF1955 0.25 -0.3 1,983,096 - +1.9 1,983,096 - -0.1 1,983,096 - -0.3 1,983,371 - GFF1956 0.62 -0.8 1,983,583 - +0.7 1,983,767 - +0.3 1,983,823 + -0.3 1,983,830 + -0.7 1,983,831 - +0.5 1,983,831 - -0.4 1,983,839 + +0.5 1,983,839 + -1.9 1,983,847 - -0.4 1,983,847 - +0.8 1,983,849 + -0.6 1,983,857 - -0.4 1,983,882 - GFF1957 0.11 +0.3 1,983,939 - GFF1957 0.16 -0.2 1,983,969 + GFF1957 0.18 -0.8 1,983,982 + GFF1957 0.20 -1.2 1,984,013 - GFF1957 0.22 -0.8 1,984,025 + GFF1957 0.23 +0.8 1,984,030 + GFF1957 0.23 +0.6 1,984,030 + GFF1957 0.23 -1.0 1,984,033 - GFF1957 0.24 -1.5 1,984,038 - GFF1957 0.24 +0.4 1,984,068 + GFF1957 0.27 +2.1 1,984,084 + GFF1957 0.28 +0.1 1,984,084 + GFF1957 0.28 -0.1 1,984,084 + GFF1957 0.28 -0.7 1,984,087 - GFF1957 0.28 -2.0 1,984,089 + GFF1957 0.28 -1.7 1,984,092 - GFF1957 0.29 +0.8 1,984,097 - GFF1957 0.29 -0.7 1,984,110 + GFF1957 0.30 -2.3 1,984,134 + GFF1957 0.32 -0.6 1,984,190 + GFF1957 0.37 -3.0 1,984,190 + GFF1957 0.37 +0.1 1,984,191 - GFF1957 0.37 -0.0 1,984,193 - GFF1957 0.37 -0.1 1,984,193 - GFF1957 0.37 -0.1 1,984,193 - GFF1957 0.37 -0.8 1,984,193 - GFF1957 0.37 -1.3 1,984,193 - GFF1957 0.37 +1.4 1,984,198 - GFF1957 0.37 +0.5 1,984,198 - GFF1957 0.37 -1.0 1,984,208 - GFF1957 0.38 +0.8 1,984,225 + GFF1957 0.39 -0.3 1,984,280 + GFF1957 0.44 +0.8 1,984,338 + GFF1957 0.49 -0.5 1,984,370 + GFF1957 0.51 -1.4 1,984,370 + GFF1957 0.51 +0.7 1,984,373 - GFF1957 0.51 +0.6 1,984,400 + GFF1957 0.54 +0.2 1,984,438 + GFF1957 0.57 +0.6 1,984,446 - GFF1957 0.57 +2.2 1,984,480 + GFF1957 0.60 -0.6 1,984,482 - GFF1957 0.60 +0.3 1,984,485 + GFF1957 0.61 +0.7 1,984,488 - GFF1957 0.61 +1.6 1,984,491 + GFF1957 0.61 +1.2 1,984,491 + GFF1957 0.61 +0.5 1,984,493 - GFF1957 0.61 -1.2 1,984,493 - GFF1957 0.61 -0.8 1,984,499 - GFF1957 0.62 +0.1 1,984,499 - GFF1957 0.62 +2.1 1,984,499 - GFF1957 0.62 -0.0 1,984,499 - GFF1957 0.62 +1.0 1,984,499 - GFF1957 0.62 +0.8 1,984,499 - GFF1957 0.62 -0.6 1,984,501 + GFF1957 0.62 +0.4 1,984,503 + GFF1957 0.62 -0.8 1,984,503 + GFF1957 0.62 -2.3 1,984,503 + GFF1957 0.62 +1.6 1,984,511 - GFF1957 0.63 +0.5 1,984,511 - GFF1957 0.63 +1.0 1,984,519 + GFF1957 0.63 +0.3 1,984,537 + GFF1957 0.65 +0.3
Or see this region's nucleotide sequence