Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1858 and GFF1859 overlap by 4 nucleotides GFF1859 and GFF1860 overlap by 1 nucleotides GFF1860 and GFF1861 are separated by 411 nucleotides GFF1861 and GFF1862 are separated by 162 nucleotides
GFF1858 - Precorrin-2 C(20)-methyltransferase (EC 2.1.1.130), at 1,887,735 to 1,888,457
GFF1858
GFF1859 - Precorrin-8X methylmutase (EC 5.4.99.61), at 1,888,454 to 1,889,083
GFF1859
GFF1860 - Precorrin-3B synthase, at 1,889,083 to 1,890,393
GFF1860
GFF1861 - tRNA-Gln-TTG, at 1,890,805 to 1,890,879
GFF1861
GFF1862 - Transcriptional regulator, MarR family, at 1,891,042 to 1,891,551
GFF1862
Position (kb)
1889
1890
1891 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1888.109 kb on - strand, within GFF1858 at 1888.138 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.146 kb on + strand, within GFF1858 at 1888.212 kb on - strand, within GFF1858 at 1888.311 kb on - strand, within GFF1858 at 1888.316 kb on - strand, within GFF1858 at 1888.418 kb on - strand at 1888.430 kb on + strand at 1888.447 kb on + strand at 1888.511 kb on - strand at 1888.532 kb on + strand, within GFF1859 at 1888.544 kb on - strand, within GFF1859 at 1888.551 kb on + strand, within GFF1859 at 1888.571 kb on - strand, within GFF1859 at 1888.628 kb on + strand, within GFF1859 at 1888.654 kb on + strand, within GFF1859 at 1888.661 kb on - strand, within GFF1859 at 1888.749 kb on + strand, within GFF1859 at 1888.749 kb on - strand, within GFF1859 at 1888.751 kb on - strand, within GFF1859 at 1888.757 kb on - strand, within GFF1859 at 1888.776 kb on + strand, within GFF1859 at 1888.781 kb on + strand, within GFF1859 at 1888.782 kb on - strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.791 kb on + strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.799 kb on - strand, within GFF1859 at 1888.822 kb on - strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.827 kb on + strand, within GFF1859 at 1888.838 kb on + strand, within GFF1859 at 1888.857 kb on + strand, within GFF1859 at 1888.888 kb on - strand, within GFF1859 at 1889.089 kb on - strand at 1889.090 kb on + strand at 1889.119 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.124 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.129 kb on + strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.132 kb on - strand at 1889.137 kb on - strand at 1889.148 kb on - strand at 1889.163 kb on - strand at 1889.178 kb on - strand at 1889.199 kb on - strand at 1889.230 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.235 kb on - strand, within GFF1860 at 1889.237 kb on - strand, within GFF1860 at 1889.247 kb on + strand, within GFF1860 at 1889.255 kb on - strand, within GFF1860 at 1889.298 kb on + strand, within GFF1860 at 1889.311 kb on - strand, within GFF1860 at 1889.338 kb on + strand, within GFF1860 at 1889.480 kb on + strand, within GFF1860 at 1889.480 kb on + strand, within GFF1860 at 1889.486 kb on - strand, within GFF1860 at 1889.487 kb on - strand, within GFF1860 at 1889.487 kb on - strand, within GFF1860 at 1889.527 kb on + strand, within GFF1860 at 1889.543 kb on + strand, within GFF1860 at 1889.543 kb on + strand, within GFF1860 at 1889.546 kb on - strand, within GFF1860 at 1889.606 kb on + strand, within GFF1860 at 1889.612 kb on - strand, within GFF1860 at 1889.673 kb on - strand, within GFF1860 at 1889.678 kb on + strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.707 kb on - strand, within GFF1860 at 1889.761 kb on + strand, within GFF1860 at 1889.785 kb on + strand, within GFF1860 at 1889.817 kb on - strand, within GFF1860 at 1889.846 kb on - strand, within GFF1860 at 1889.860 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.868 kb on - strand, within GFF1860 at 1889.914 kb on - strand, within GFF1860 at 1889.914 kb on - strand, within GFF1860 at 1889.929 kb on + strand, within GFF1860 at 1889.932 kb on - strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.934 kb on + strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.942 kb on - strand, within GFF1860 at 1889.949 kb on - strand, within GFF1860 at 1889.982 kb on - strand, within GFF1860 at 1889.992 kb on + strand, within GFF1860 at 1890.204 kb on + strand, within GFF1860 at 1890.204 kb on + strand, within GFF1860 at 1890.204 kb on + strand, within GFF1860 at 1890.208 kb on + strand, within GFF1860 at 1890.212 kb on - strand, within GFF1860 at 1890.212 kb on - strand, within GFF1860 at 1890.236 kb on + strand, within GFF1860 at 1890.236 kb on + strand, within GFF1860 at 1890.238 kb on + strand, within GFF1860 at 1890.244 kb on - strand, within GFF1860 at 1890.321 kb on + strand at 1890.411 kb on + strand at 1890.419 kb on - strand at 1890.419 kb on - strand at 1890.419 kb on - strand at 1890.465 kb on + strand at 1890.465 kb on + strand at 1890.473 kb on - strand at 1890.488 kb on + strand at 1890.488 kb on + strand at 1890.491 kb on - strand at 1890.496 kb on - strand at 1890.496 kb on - strand at 1890.562 kb on - strand at 1890.575 kb on - strand at 1890.577 kb on + strand at 1890.577 kb on - strand at 1890.596 kb on - strand at 1890.617 kb on + strand at 1890.659 kb on - strand at 1890.693 kb on - strand at 1890.964 kb on - strand at 1890.964 kb on - strand at 1891.011 kb on + strand at 1891.015 kb on + strand at 1891.017 kb on - strand at 1891.021 kb on - strand at 1891.036 kb on + strand at 1891.056 kb on + strand at 1891.056 kb on - strand at 1891.056 kb on - strand at 1891.113 kb on - strand, within GFF1862 at 1891.126 kb on + strand, within GFF1862 at 1891.145 kb on + strand, within GFF1862 at 1891.145 kb on + strand, within GFF1862 at 1891.153 kb on - strand, within GFF1862 at 1891.163 kb on - strand, within GFF1862 at 1891.189 kb on + strand, within GFF1862 at 1891.189 kb on + strand, within GFF1862 at 1891.189 kb on + strand, within GFF1862 at 1891.197 kb on - strand, within GFF1862 at 1891.228 kb on - strand, within GFF1862 at 1891.235 kb on + strand, within GFF1862 at 1891.241 kb on + strand, within GFF1862 at 1891.241 kb on + strand, within GFF1862 at 1891.243 kb on - strand, within GFF1862 at 1891.255 kb on + strand, within GFF1862 at 1891.255 kb on + strand, within GFF1862 at 1891.255 kb on + strand, within GFF1862 at 1891.295 kb on + strand, within GFF1862 at 1891.303 kb on - strand, within GFF1862 at 1891.303 kb on - strand, within GFF1862 at 1891.325 kb on + strand, within GFF1862 at 1891.330 kb on + strand, within GFF1862 at 1891.333 kb on - strand, within GFF1862 at 1891.333 kb on - strand, within GFF1862 at 1891.338 kb on - strand, within GFF1862 at 1891.384 kb on - strand, within GFF1862
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,888,109 - GFF1858 0.52 -0.1 1,888,138 + GFF1858 0.56 -0.5 1,888,146 + GFF1858 0.57 +0.5 1,888,146 + GFF1858 0.57 -1.9 1,888,212 - GFF1858 0.66 -0.6 1,888,311 - GFF1858 0.80 -1.7 1,888,316 - GFF1858 0.80 -0.7 1,888,418 - -0.7 1,888,430 + -0.5 1,888,447 + -0.5 1,888,511 - -2.8 1,888,532 + GFF1859 0.12 +1.3 1,888,544 - GFF1859 0.14 +0.5 1,888,551 + GFF1859 0.15 -0.8 1,888,571 - GFF1859 0.19 -1.6 1,888,628 + GFF1859 0.28 +0.4 1,888,654 + GFF1859 0.32 +1.9 1,888,661 - GFF1859 0.33 +0.9 1,888,749 + GFF1859 0.47 -0.6 1,888,749 - GFF1859 0.47 +0.5 1,888,751 - GFF1859 0.47 -0.6 1,888,757 - GFF1859 0.48 -1.0 1,888,776 + GFF1859 0.51 -1.3 1,888,781 + GFF1859 0.52 +0.2 1,888,782 - GFF1859 0.52 -0.7 1,888,791 + GFF1859 0.53 -0.0 1,888,791 + GFF1859 0.53 -2.1 1,888,799 - GFF1859 0.55 -0.3 1,888,799 - GFF1859 0.55 -0.8 1,888,799 - GFF1859 0.55 +0.9 1,888,799 - GFF1859 0.55 +1.2 1,888,822 - GFF1859 0.58 +1.5 1,888,827 + GFF1859 0.59 -2.4 1,888,827 + GFF1859 0.59 -1.0 1,888,838 + GFF1859 0.61 +0.5 1,888,857 + GFF1859 0.64 -2.6 1,888,888 - GFF1859 0.69 +0.5 1,889,089 - +2.2 1,889,090 + -0.4 1,889,119 + -1.2 1,889,124 + +2.2 1,889,124 + -2.1 1,889,124 + -1.0 1,889,124 + -1.0 1,889,124 + -0.8 1,889,124 + -0.7 1,889,129 + -1.8 1,889,129 + -0.9 1,889,129 + +2.2 1,889,132 - +0.4 1,889,132 - +0.3 1,889,132 - +0.8 1,889,132 - -0.0 1,889,132 - -1.7 1,889,137 - +1.2 1,889,148 - -0.8 1,889,163 - +0.5 1,889,178 - -1.1 1,889,199 - -1.4 1,889,230 - GFF1860 0.11 +0.5 1,889,235 - GFF1860 0.12 +0.3 1,889,235 - GFF1860 0.12 +0.2 1,889,237 - GFF1860 0.12 +1.2 1,889,247 + GFF1860 0.13 -0.3 1,889,255 - GFF1860 0.13 -0.8 1,889,298 + GFF1860 0.16 -0.7 1,889,311 - GFF1860 0.17 +2.0 1,889,338 + GFF1860 0.19 -0.5 1,889,480 + GFF1860 0.30 -1.3 1,889,480 + GFF1860 0.30 -0.6 1,889,486 - GFF1860 0.31 +0.9 1,889,487 - GFF1860 0.31 +0.1 1,889,487 - GFF1860 0.31 -1.0 1,889,527 + GFF1860 0.34 +0.3 1,889,543 + GFF1860 0.35 -0.3 1,889,543 + GFF1860 0.35 -0.6 1,889,546 - GFF1860 0.35 -1.9 1,889,606 + GFF1860 0.40 +1.3 1,889,612 - GFF1860 0.40 +0.0 1,889,673 - GFF1860 0.45 -2.1 1,889,678 + GFF1860 0.45 -2.0 1,889,707 - GFF1860 0.48 -0.7 1,889,707 - GFF1860 0.48 +0.5 1,889,707 - GFF1860 0.48 -1.4 1,889,761 + GFF1860 0.52 +1.2 1,889,785 + GFF1860 0.54 -1.7 1,889,817 - GFF1860 0.56 -1.6 1,889,846 - GFF1860 0.58 +1.9 1,889,860 - GFF1860 0.59 -0.6 1,889,868 - GFF1860 0.60 +1.1 1,889,868 - GFF1860 0.60 -0.8 1,889,868 - GFF1860 0.60 +0.9 1,889,914 - GFF1860 0.63 +0.7 1,889,914 - GFF1860 0.63 -2.3 1,889,929 + GFF1860 0.65 -2.2 1,889,932 - GFF1860 0.65 -0.7 1,889,934 + GFF1860 0.65 -0.7 1,889,934 + GFF1860 0.65 -1.4 1,889,934 + GFF1860 0.65 -0.1 1,889,934 + GFF1860 0.65 -1.8 1,889,934 + GFF1860 0.65 -1.4 1,889,934 + GFF1860 0.65 -0.5 1,889,934 + GFF1860 0.65 -1.1 1,889,934 + GFF1860 0.65 +1.8 1,889,934 + GFF1860 0.65 -1.1 1,889,934 + GFF1860 0.65 +1.6 1,889,934 + GFF1860 0.65 +0.9 1,889,942 - GFF1860 0.66 -2.2 1,889,942 - GFF1860 0.66 +0.6 1,889,942 - GFF1860 0.66 +0.3 1,889,942 - GFF1860 0.66 -1.5 1,889,942 - GFF1860 0.66 -2.1 1,889,949 - GFF1860 0.66 -1.0 1,889,982 - GFF1860 0.69 -1.7 1,889,992 + GFF1860 0.69 +0.5 1,890,204 + GFF1860 0.86 -0.3 1,890,204 + GFF1860 0.86 +1.3 1,890,204 + GFF1860 0.86 -1.9 1,890,208 + GFF1860 0.86 +1.0 1,890,212 - GFF1860 0.86 +0.9 1,890,212 - GFF1860 0.86 -2.2 1,890,236 + GFF1860 0.88 +0.1 1,890,236 + GFF1860 0.88 -0.5 1,890,238 + GFF1860 0.88 -1.7 1,890,244 - GFF1860 0.89 -1.0 1,890,321 + -0.3 1,890,411 + +0.3 1,890,419 - -0.7 1,890,419 - +0.4 1,890,419 - -0.4 1,890,465 + +0.3 1,890,465 + +0.6 1,890,473 - -0.3 1,890,488 + -1.2 1,890,488 + -0.7 1,890,491 - -0.9 1,890,496 - +0.5 1,890,496 - -1.1 1,890,562 - +1.0 1,890,575 - +1.9 1,890,577 + +0.3 1,890,577 - -0.8 1,890,596 - +0.1 1,890,617 + -1.5 1,890,659 - -0.1 1,890,693 - -0.1 1,890,964 - -0.1 1,890,964 - -0.1 1,891,011 + -0.8 1,891,015 + +0.4 1,891,017 - -0.5 1,891,021 - +1.3 1,891,036 + -2.0 1,891,056 + +0.1 1,891,056 - +0.2 1,891,056 - +0.8 1,891,113 - GFF1862 0.14 -1.5 1,891,126 + GFF1862 0.16 -1.7 1,891,145 + GFF1862 0.20 +1.8 1,891,145 + GFF1862 0.20 -0.9 1,891,153 - GFF1862 0.22 +1.1 1,891,163 - GFF1862 0.24 +0.1 1,891,189 + GFF1862 0.29 +1.2 1,891,189 + GFF1862 0.29 +1.3 1,891,189 + GFF1862 0.29 +0.8 1,891,197 - GFF1862 0.30 -0.8 1,891,228 - GFF1862 0.36 +0.2 1,891,235 + GFF1862 0.38 +1.0 1,891,241 + GFF1862 0.39 +1.7 1,891,241 + GFF1862 0.39 +0.3 1,891,243 - GFF1862 0.39 +1.0 1,891,255 + GFF1862 0.42 +1.4 1,891,255 + GFF1862 0.42 +0.6 1,891,255 + GFF1862 0.42 +0.6 1,891,295 + GFF1862 0.50 +1.5 1,891,303 - GFF1862 0.51 +1.2 1,891,303 - GFF1862 0.51 -0.3 1,891,325 + GFF1862 0.55 +0.4 1,891,330 + GFF1862 0.56 -0.3 1,891,333 - GFF1862 0.57 -0.1 1,891,333 - GFF1862 0.57 -1.4 1,891,338 - GFF1862 0.58 -0.1 1,891,384 - GFF1862 0.67 +0.1
Or see this region's nucleotide sequence