Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1853 and GFF1854 are separated by 2 nucleotides GFF1854 and GFF1855 overlap by 4 nucleotides GFF1855 and GFF1856 are separated by 46 nucleotides GFF1856 and GFF1857 overlap by 40 nucleotides
GFF1853 - Precorrin-4 C(11)-methyltransferase (EC 2.1.1.133), at 1,883,883 to 1,884,668
GFF1853
GFF1854 - Cobalamin biosynthesis protein CobE, at 1,884,671 to 1,885,027
GFF1854
GFF1855 - Precorrin-6Y C(5,15)-methyltransferase [decarboxylating] (EC 2.1.1.132), at 1,885,024 to 1,886,175
GFF1855
GFF1856 - Precorrin-6A reductase (EC 1.3.1.54), at 1,886,222 to 1,886,995
GFF1856
GFF1857 - Precorrin-3B C(17)-methyltransferase (EC 2.1.1.131), at 1,886,956 to 1,887,738
GFF1857
Position (kb)
1885
1886
1887 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.025 kb on - strand, within GFF1853 at 1884.040 kb on + strand, within GFF1853 at 1884.077 kb on + strand, within GFF1853 at 1884.085 kb on - strand, within GFF1853 at 1884.112 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.145 kb on + strand, within GFF1853 at 1884.153 kb on - strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.183 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.238 kb on + strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.246 kb on - strand, within GFF1853 at 1884.248 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.283 kb on + strand, within GFF1853 at 1884.284 kb on + strand, within GFF1853 at 1884.314 kb on - strand, within GFF1853 at 1884.437 kb on + strand, within GFF1853 at 1884.439 kb on + strand, within GFF1853 at 1884.487 kb on + strand, within GFF1853 at 1884.494 kb on + strand, within GFF1853 at 1884.508 kb on + strand, within GFF1853 at 1884.516 kb on - strand, within GFF1853 at 1884.541 kb on - strand, within GFF1853 at 1884.579 kb on - strand, within GFF1853 at 1884.677 kb on + strand at 1884.678 kb on + strand at 1884.678 kb on - strand at 1884.719 kb on + strand, within GFF1854 at 1884.727 kb on - strand, within GFF1854 at 1884.765 kb on + strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.771 kb on - strand, within GFF1854 at 1884.784 kb on + strand, within GFF1854 at 1884.828 kb on - strand, within GFF1854 at 1884.838 kb on - strand, within GFF1854 at 1884.911 kb on - strand, within GFF1854 at 1884.979 kb on + strand, within GFF1854 at 1885.034 kb on + strand at 1885.037 kb on - strand at 1885.039 kb on + strand at 1885.102 kb on - strand at 1885.102 kb on - strand at 1885.113 kb on + strand at 1885.113 kb on + strand at 1885.142 kb on - strand, within GFF1855 at 1885.164 kb on + strand, within GFF1855 at 1885.191 kb on + strand, within GFF1855 at 1885.199 kb on - strand, within GFF1855 at 1885.235 kb on + strand, within GFF1855 at 1885.255 kb on + strand, within GFF1855 at 1885.317 kb on + strand, within GFF1855 at 1885.346 kb on - strand, within GFF1855 at 1885.357 kb on + strand, within GFF1855 at 1885.357 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.372 kb on + strand, within GFF1855 at 1885.376 kb on + strand, within GFF1855 at 1885.380 kb on - strand, within GFF1855 at 1885.381 kb on + strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.389 kb on - strand, within GFF1855 at 1885.404 kb on - strand, within GFF1855 at 1885.467 kb on - strand, within GFF1855 at 1885.500 kb on + strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.508 kb on - strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.576 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.581 kb on + strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.584 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.589 kb on - strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.602 kb on + strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.610 kb on - strand, within GFF1855 at 1885.694 kb on - strand, within GFF1855 at 1885.823 kb on + strand, within GFF1855 at 1885.866 kb on + strand, within GFF1855 at 1885.911 kb on - strand, within GFF1855 at 1885.935 kb on - strand, within GFF1855 at 1885.957 kb on + strand, within GFF1855 at 1885.997 kb on + strand, within GFF1855 at 1886.013 kb on + strand, within GFF1855 at 1886.061 kb on - strand at 1886.079 kb on + strand at 1886.097 kb on + strand at 1886.112 kb on - strand at 1886.112 kb on - strand at 1886.112 kb on - strand at 1886.145 kb on - strand at 1886.177 kb on - strand at 1886.199 kb on + strand at 1886.216 kb on + strand at 1886.224 kb on - strand at 1886.241 kb on - strand at 1886.259 kb on + strand at 1886.259 kb on - strand at 1886.294 kb on - strand at 1886.312 kb on + strand, within GFF1856 at 1886.320 kb on - strand, within GFF1856 at 1886.320 kb on - strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.353 kb on + strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.361 kb on - strand, within GFF1856 at 1886.389 kb on + strand, within GFF1856 at 1886.406 kb on - strand, within GFF1856 at 1886.421 kb on + strand, within GFF1856 at 1886.434 kb on + strand, within GFF1856 at 1886.439 kb on + strand, within GFF1856 at 1886.444 kb on + strand, within GFF1856 at 1886.444 kb on + strand, within GFF1856 at 1886.452 kb on - strand, within GFF1856 at 1886.487 kb on - strand, within GFF1856 at 1886.516 kb on + strand, within GFF1856 at 1886.522 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.524 kb on - strand, within GFF1856 at 1886.531 kb on - strand, within GFF1856 at 1886.554 kb on + strand, within GFF1856 at 1886.575 kb on - strand, within GFF1856 at 1886.630 kb on - strand, within GFF1856 at 1886.651 kb on - strand, within GFF1856 at 1886.711 kb on + strand, within GFF1856 at 1886.727 kb on + strand, within GFF1856 at 1886.735 kb on - strand, within GFF1856 at 1886.735 kb on - strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.772 kb on + strand, within GFF1856 at 1886.777 kb on + strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.780 kb on - strand, within GFF1856 at 1886.848 kb on + strand, within GFF1856 at 1886.848 kb on + strand, within GFF1856 at 1886.856 kb on - strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.865 kb on + strand, within GFF1856 at 1886.869 kb on + strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.873 kb on - strand, within GFF1856 at 1886.877 kb on - strand, within GFF1856 at 1886.912 kb on + strand, within GFF1856 at 1886.918 kb on + strand at 1886.929 kb on + strand at 1886.931 kb on + strand at 1886.939 kb on + strand at 1886.947 kb on - strand at 1886.947 kb on - strand at 1886.965 kb on - strand at 1886.970 kb on + strand at 1886.990 kb on + strand at 1886.996 kb on - strand at 1886.996 kb on - strand at 1886.997 kb on + strand at 1886.997 kb on + strand at 1886.997 kb on + strand at 1886.998 kb on - strand at 1887.000 kb on - strand at 1887.011 kb on - strand at 1887.028 kb on + strand at 1887.036 kb on - strand, within GFF1857 at 1887.080 kb on - strand, within GFF1857 at 1887.090 kb on - strand, within GFF1857 at 1887.136 kb on + strand, within GFF1857
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,884,025 - GFF1853 0.18 -0.8 1,884,025 - GFF1853 0.18 +0.7 1,884,025 - GFF1853 0.18 -0.7 1,884,025 - GFF1853 0.18 -0.4 1,884,040 + GFF1853 0.20 +0.6 1,884,077 + GFF1853 0.25 +0.2 1,884,085 - GFF1853 0.26 +0.4 1,884,112 + GFF1853 0.29 +0.5 1,884,145 + GFF1853 0.33 -1.5 1,884,145 + GFF1853 0.33 -0.5 1,884,145 + GFF1853 0.33 +0.5 1,884,153 - GFF1853 0.34 -1.2 1,884,183 + GFF1853 0.38 -1.1 1,884,183 + GFF1853 0.38 +1.7 1,884,238 + GFF1853 0.45 -0.5 1,884,238 + GFF1853 0.45 -1.4 1,884,238 + GFF1853 0.45 -0.7 1,884,238 + GFF1853 0.45 -0.7 1,884,238 + GFF1853 0.45 +1.1 1,884,238 + GFF1853 0.45 -0.1 1,884,246 - GFF1853 0.46 -1.1 1,884,246 - GFF1853 0.46 -0.5 1,884,246 - GFF1853 0.46 -1.3 1,884,248 + GFF1853 0.46 -1.3 1,884,283 + GFF1853 0.51 -0.7 1,884,283 + GFF1853 0.51 -1.4 1,884,284 + GFF1853 0.51 +0.5 1,884,314 - GFF1853 0.55 -1.5 1,884,437 + GFF1853 0.70 +0.6 1,884,439 + GFF1853 0.71 -0.8 1,884,487 + GFF1853 0.77 +0.7 1,884,494 + GFF1853 0.78 -0.2 1,884,508 + GFF1853 0.80 -0.4 1,884,516 - GFF1853 0.81 -0.7 1,884,541 - GFF1853 0.84 -0.3 1,884,579 - GFF1853 0.89 +0.5 1,884,677 + -0.2 1,884,678 + -0.3 1,884,678 - +1.2 1,884,719 + GFF1854 0.13 -0.7 1,884,727 - GFF1854 0.16 -0.4 1,884,765 + GFF1854 0.26 -1.5 1,884,771 - GFF1854 0.28 -1.9 1,884,771 - GFF1854 0.28 -0.7 1,884,784 + GFF1854 0.32 +0.7 1,884,828 - GFF1854 0.44 -0.1 1,884,838 - GFF1854 0.47 +0.9 1,884,911 - GFF1854 0.67 -1.8 1,884,979 + GFF1854 0.86 +1.0 1,885,034 + -0.7 1,885,037 - -0.7 1,885,039 + -1.4 1,885,102 - -1.2 1,885,102 - +0.5 1,885,113 + +0.0 1,885,113 + -1.4 1,885,142 - GFF1855 0.10 -0.3 1,885,164 + GFF1855 0.12 +0.2 1,885,191 + GFF1855 0.14 -0.8 1,885,199 - GFF1855 0.15 -2.1 1,885,235 + GFF1855 0.18 -0.1 1,885,255 + GFF1855 0.20 -0.7 1,885,317 + GFF1855 0.25 -0.8 1,885,346 - GFF1855 0.28 +0.4 1,885,357 + GFF1855 0.29 -0.5 1,885,357 + GFF1855 0.29 -1.1 1,885,372 + GFF1855 0.30 -0.4 1,885,372 + GFF1855 0.30 -0.1 1,885,376 + GFF1855 0.31 +0.5 1,885,380 - GFF1855 0.31 -1.8 1,885,381 + GFF1855 0.31 -1.0 1,885,389 - GFF1855 0.32 -1.8 1,885,389 - GFF1855 0.32 -0.9 1,885,404 - GFF1855 0.33 -1.0 1,885,467 - GFF1855 0.38 +1.8 1,885,500 + GFF1855 0.41 -1.4 1,885,508 - GFF1855 0.42 -1.2 1,885,508 - GFF1855 0.42 -1.1 1,885,508 - GFF1855 0.42 +1.3 1,885,576 + GFF1855 0.48 -0.5 1,885,576 + GFF1855 0.48 -2.5 1,885,576 + GFF1855 0.48 -1.2 1,885,576 + GFF1855 0.48 -0.7 1,885,576 + GFF1855 0.48 -1.0 1,885,581 + GFF1855 0.48 -0.1 1,885,581 + GFF1855 0.48 -1.1 1,885,581 + GFF1855 0.48 +0.2 1,885,584 - GFF1855 0.49 -1.7 1,885,584 - GFF1855 0.49 -1.0 1,885,589 - GFF1855 0.49 -0.3 1,885,589 - GFF1855 0.49 +1.1 1,885,589 - GFF1855 0.49 +0.3 1,885,589 - GFF1855 0.49 -0.9 1,885,602 + GFF1855 0.50 -0.2 1,885,602 + GFF1855 0.50 -0.7 1,885,602 + GFF1855 0.50 -1.7 1,885,602 + GFF1855 0.50 -1.4 1,885,610 - GFF1855 0.51 +0.3 1,885,610 - GFF1855 0.51 -1.0 1,885,610 - GFF1855 0.51 -1.1 1,885,610 - GFF1855 0.51 -0.2 1,885,694 - GFF1855 0.58 -1.7 1,885,823 + GFF1855 0.69 -1.0 1,885,866 + GFF1855 0.73 -0.1 1,885,911 - GFF1855 0.77 -2.0 1,885,935 - GFF1855 0.79 +0.2 1,885,957 + GFF1855 0.81 +0.5 1,885,997 + GFF1855 0.84 +1.2 1,886,013 + GFF1855 0.86 -0.3 1,886,061 - -0.2 1,886,079 + -0.3 1,886,097 + -2.0 1,886,112 - +0.4 1,886,112 - -1.1 1,886,112 - -1.2 1,886,145 - -0.5 1,886,177 - -0.2 1,886,199 + -0.5 1,886,216 + -1.4 1,886,224 - +0.7 1,886,241 - -1.6 1,886,259 + +0.3 1,886,259 - +0.6 1,886,294 - +0.3 1,886,312 + GFF1856 0.12 -1.3 1,886,320 - GFF1856 0.13 -0.8 1,886,320 - GFF1856 0.13 -2.4 1,886,353 + GFF1856 0.17 -2.0 1,886,353 + GFF1856 0.17 -1.4 1,886,353 + GFF1856 0.17 -0.8 1,886,353 + GFF1856 0.17 -2.0 1,886,353 + GFF1856 0.17 -0.1 1,886,353 + GFF1856 0.17 -1.4 1,886,353 + GFF1856 0.17 -0.4 1,886,353 + GFF1856 0.17 +0.9 1,886,353 + GFF1856 0.17 +0.5 1,886,353 + GFF1856 0.17 -0.9 1,886,353 + GFF1856 0.17 +1.2 1,886,353 + GFF1856 0.17 -1.1 1,886,353 + GFF1856 0.17 -1.5 1,886,361 - GFF1856 0.18 +1.9 1,886,361 - GFF1856 0.18 +1.4 1,886,361 - GFF1856 0.18 -0.1 1,886,361 - GFF1856 0.18 +1.9 1,886,361 - GFF1856 0.18 -0.8 1,886,361 - GFF1856 0.18 -1.1 1,886,389 + GFF1856 0.22 +0.9 1,886,406 - GFF1856 0.24 +1.4 1,886,421 + GFF1856 0.26 -1.5 1,886,434 + GFF1856 0.27 +0.5 1,886,439 + GFF1856 0.28 +0.3 1,886,444 + GFF1856 0.29 -0.8 1,886,444 + GFF1856 0.29 +1.3 1,886,452 - GFF1856 0.30 -0.5 1,886,487 - GFF1856 0.34 -0.7 1,886,516 + GFF1856 0.38 -0.9 1,886,522 - GFF1856 0.39 -0.5 1,886,524 - GFF1856 0.39 -1.3 1,886,524 - GFF1856 0.39 -2.6 1,886,524 - GFF1856 0.39 +2.7 1,886,524 - GFF1856 0.39 -0.2 1,886,524 - GFF1856 0.39 +1.8 1,886,524 - GFF1856 0.39 +1.3 1,886,531 - GFF1856 0.40 -1.1 1,886,554 + GFF1856 0.43 +1.0 1,886,575 - GFF1856 0.46 -1.7 1,886,630 - GFF1856 0.53 -0.5 1,886,651 - GFF1856 0.55 +0.7 1,886,711 + GFF1856 0.63 -2.5 1,886,727 + GFF1856 0.65 +1.0 1,886,735 - GFF1856 0.66 -1.6 1,886,735 - GFF1856 0.66 -0.8 1,886,772 + GFF1856 0.71 +0.9 1,886,772 + GFF1856 0.71 -1.0 1,886,772 + GFF1856 0.71 +0.4 1,886,777 + GFF1856 0.72 -0.1 1,886,780 - GFF1856 0.72 +1.3 1,886,780 - GFF1856 0.72 +2.1 1,886,780 - GFF1856 0.72 -0.5 1,886,780 - GFF1856 0.72 +0.9 1,886,780 - GFF1856 0.72 -0.3 1,886,780 - GFF1856 0.72 -1.3 1,886,848 + GFF1856 0.81 -1.3 1,886,848 + GFF1856 0.81 -2.4 1,886,856 - GFF1856 0.82 +0.2 1,886,865 + GFF1856 0.83 -0.5 1,886,865 + GFF1856 0.83 -1.1 1,886,865 + GFF1856 0.83 -0.3 1,886,865 + GFF1856 0.83 -2.1 1,886,865 + GFF1856 0.83 -0.1 1,886,869 + GFF1856 0.84 +0.3 1,886,873 - GFF1856 0.84 +0.8 1,886,873 - GFF1856 0.84 +0.2 1,886,873 - GFF1856 0.84 -0.9 1,886,877 - GFF1856 0.85 -0.3 1,886,912 + GFF1856 0.89 -0.5 1,886,918 + -1.5 1,886,929 + -1.9 1,886,931 + -1.2 1,886,939 + +0.9 1,886,947 - -0.8 1,886,947 - -1.2 1,886,965 - -0.4 1,886,970 + +0.5 1,886,990 + +1.2 1,886,996 - +0.8 1,886,996 - +0.0 1,886,997 + -0.5 1,886,997 + -0.4 1,886,997 + +0.2 1,886,998 - -1.4 1,887,000 - +0.0 1,887,011 - +1.0 1,887,028 + -1.4 1,887,036 - GFF1857 0.10 +0.3 1,887,080 - GFF1857 0.16 -0.5 1,887,090 - GFF1857 0.17 +1.2 1,887,136 + GFF1857 0.23 +0.0
Or see this region's nucleotide sequence