Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF218 and GFF219 are separated by 213 nucleotides GFF219 and GFF220 are separated by 83 nucleotides GFF220 and GFF221 are separated by 56 nucleotides GFF221 and GFF222 are separated by 183 nucleotides
GFF218 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 225,402 to 226,373
GFF218
GFF219 - hypothetical protein, at 226,587 to 226,805
GFF219
GFF220 - hypothetical protein, at 226,889 to 227,122
GFF220
GFF221 - hypothetical protein, at 227,179 to 227,604
GFF221
GFF222 - hypothetical protein, at 227,788 to 228,771
GFF222
Position (kb)
226
227
228 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 225.933 kb on + strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.941 kb on - strand, within GFF218 at 225.973 kb on + strand, within GFF218 at 226.026 kb on + strand, within GFF218 at 226.034 kb on - strand, within GFF218 at 226.071 kb on + strand, within GFF218 at 226.075 kb on - strand, within GFF218 at 226.079 kb on - strand, within GFF218 at 226.094 kb on - strand, within GFF218 at 226.129 kb on - strand, within GFF218 at 226.141 kb on + strand, within GFF218 at 226.148 kb on - strand, within GFF218 at 226.148 kb on - strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.154 kb on + strand, within GFF218 at 226.249 kb on + strand, within GFF218 at 226.295 kb on - strand at 226.300 kb on + strand at 226.308 kb on - strand at 226.318 kb on - strand at 226.353 kb on - strand at 226.450 kb on + strand at 226.458 kb on - strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.480 kb on + strand at 226.483 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.488 kb on - strand at 226.490 kb on + strand at 226.490 kb on + strand at 226.498 kb on - strand at 226.498 kb on - strand at 226.498 kb on - strand at 226.558 kb on - strand at 226.578 kb on + strand at 226.578 kb on + strand at 226.585 kb on - strand at 226.589 kb on + strand at 226.596 kb on - strand at 226.639 kb on - strand, within GFF219 at 226.639 kb on - strand, within GFF219 at 226.639 kb on - strand, within GFF219 at 226.671 kb on + strand, within GFF219 at 226.679 kb on - strand, within GFF219 at 226.692 kb on + strand, within GFF219 at 226.692 kb on + strand, within GFF219 at 226.700 kb on - strand, within GFF219 at 226.700 kb on - strand, within GFF219 at 226.747 kb on - strand, within GFF219 at 226.748 kb on + strand, within GFF219 at 226.843 kb on - strand at 226.861 kb on + strand at 226.887 kb on - strand at 226.904 kb on + strand at 226.907 kb on - strand at 226.907 kb on - strand at 226.920 kb on - strand, within GFF220 at 226.933 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.948 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.976 kb on + strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.984 kb on - strand, within GFF220 at 226.990 kb on + strand, within GFF220 at 226.993 kb on + strand, within GFF220 at 227.001 kb on - strand, within GFF220 at 227.060 kb on - strand, within GFF220 at 227.061 kb on - strand, within GFF220 at 227.068 kb on + strand, within GFF220 at 227.085 kb on - strand, within GFF220 at 227.104 kb on + strand at 227.112 kb on - strand at 227.152 kb on + strand at 227.160 kb on - strand at 227.160 kb on - strand at 227.170 kb on + strand at 227.175 kb on - strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.177 kb on + strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.185 kb on - strand at 227.270 kb on + strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.283 kb on - strand, within GFF221 at 227.286 kb on + strand, within GFF221 at 227.294 kb on - strand, within GFF221 at 227.301 kb on + strand, within GFF221 at 227.326 kb on + strand, within GFF221 at 227.326 kb on - strand, within GFF221 at 227.334 kb on - strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.339 kb on + strand, within GFF221 at 227.343 kb on + strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.351 kb on - strand, within GFF221 at 227.437 kb on - strand, within GFF221 at 227.444 kb on + strand, within GFF221 at 227.458 kb on - strand, within GFF221 at 227.474 kb on + strand, within GFF221 at 227.474 kb on + strand, within GFF221 at 227.482 kb on - strand, within GFF221 at 227.490 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.503 kb on + strand, within GFF221 at 227.511 kb on - strand, within GFF221 at 227.511 kb on - strand, within GFF221 at 227.517 kb on - strand, within GFF221 at 227.549 kb on - strand, within GFF221 at 227.553 kb on - strand, within GFF221 at 227.553 kb on - strand, within GFF221 at 227.558 kb on + strand, within GFF221 at 227.566 kb on - strand at 227.576 kb on - strand at 227.587 kb on - strand at 227.606 kb on + strand at 227.614 kb on - strand at 227.619 kb on - strand at 227.637 kb on + strand at 227.669 kb on + strand at 227.669 kb on + strand at 227.677 kb on - strand at 227.718 kb on + strand at 227.726 kb on - strand at 227.726 kb on - strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.745 kb on + strand at 227.750 kb on + strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.753 kb on - strand at 227.779 kb on - strand at 227.792 kb on + strand at 227.792 kb on + strand at 227.792 kb on + strand at 227.800 kb on - strand at 227.800 kb on - strand at 227.826 kb on + strand at 227.834 kb on - strand at 227.847 kb on + strand at 227.847 kb on + strand at 227.868 kb on + strand at 227.868 kb on + strand at 227.868 kb on + strand at 227.880 kb on + strand at 227.883 kb on + strand at 227.905 kb on + strand, within GFF222 at 227.907 kb on + strand, within GFF222 at 227.913 kb on + strand, within GFF222 at 227.946 kb on + strand, within GFF222 at 227.954 kb on - strand, within GFF222 at 227.976 kb on + strand, within GFF222 at 228.020 kb on - strand, within GFF222 at 228.025 kb on + strand, within GFF222 at 228.037 kb on + strand, within GFF222 at 228.047 kb on + strand, within GFF222 at 228.047 kb on + strand, within GFF222 at 228.050 kb on - strand, within GFF222 at 228.057 kb on + strand, within GFF222 at 228.073 kb on - strand, within GFF222 at 228.093 kb on + strand, within GFF222 at 228.100 kb on - strand, within GFF222
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 225,933 + GFF218 0.55 -1.9 225,941 - GFF218 0.55 -0.0 225,941 - GFF218 0.55 -0.2 225,941 - GFF218 0.55 -2.2 225,973 + GFF218 0.59 -0.6 226,026 + GFF218 0.64 +0.2 226,034 - GFF218 0.65 +0.9 226,071 + GFF218 0.69 -1.7 226,075 - GFF218 0.69 -0.7 226,079 - GFF218 0.70 -0.9 226,094 - GFF218 0.71 +0.1 226,129 - GFF218 0.75 -1.4 226,141 + GFF218 0.76 +0.4 226,148 - GFF218 0.77 +0.4 226,148 - GFF218 0.77 -0.3 226,154 + GFF218 0.77 -1.0 226,154 + GFF218 0.77 -1.5 226,154 + GFF218 0.77 -1.2 226,249 + GFF218 0.87 +0.2 226,295 - +1.0 226,300 + -0.5 226,308 - +0.9 226,318 - +0.3 226,353 - +0.2 226,450 + -1.6 226,458 - +0.3 226,480 + -3.0 226,480 + -0.5 226,480 + +1.0 226,480 + +0.4 226,480 + +1.0 226,480 + +1.0 226,483 - +0.1 226,488 - +1.8 226,488 - +1.2 226,488 - -0.5 226,488 - +0.2 226,488 - +0.1 226,488 - +0.7 226,488 - -0.2 226,490 + +0.8 226,490 + +0.2 226,498 - -0.9 226,498 - +0.7 226,498 - +1.6 226,558 - -0.6 226,578 + +0.7 226,578 + -0.1 226,585 - +0.5 226,589 + -0.5 226,596 - -0.2 226,639 - GFF219 0.24 -0.4 226,639 - GFF219 0.24 -1.2 226,639 - GFF219 0.24 +0.8 226,671 + GFF219 0.38 +0.8 226,679 - GFF219 0.42 +0.5 226,692 + GFF219 0.48 -1.1 226,692 + GFF219 0.48 -0.2 226,700 - GFF219 0.52 +0.8 226,700 - GFF219 0.52 +0.1 226,747 - GFF219 0.73 +2.8 226,748 + GFF219 0.74 +0.5 226,843 - -0.9 226,861 + -1.9 226,887 - -0.3 226,904 + +1.7 226,907 - -1.8 226,907 - -0.3 226,920 - GFF220 0.13 +0.0 226,933 + GFF220 0.19 -0.3 226,948 + GFF220 0.25 +0.6 226,948 + GFF220 0.25 +0.0 226,976 + GFF220 0.37 +0.6 226,976 + GFF220 0.37 +1.3 226,976 + GFF220 0.37 +0.7 226,976 + GFF220 0.37 -0.9 226,976 + GFF220 0.37 +0.1 226,976 + GFF220 0.37 -0.7 226,976 + GFF220 0.37 -0.6 226,976 + GFF220 0.37 -2.2 226,976 + GFF220 0.37 +0.6 226,984 - GFF220 0.41 -1.2 226,984 - GFF220 0.41 -0.9 226,984 - GFF220 0.41 +0.1 226,984 - GFF220 0.41 -1.2 226,984 - GFF220 0.41 +0.1 226,984 - GFF220 0.41 -0.8 226,990 + GFF220 0.43 -2.3 226,993 + GFF220 0.44 +0.6 227,001 - GFF220 0.48 -0.5 227,060 - GFF220 0.73 +0.4 227,061 - GFF220 0.74 -0.5 227,068 + GFF220 0.76 -1.1 227,085 - GFF220 0.84 -1.5 227,104 + -2.5 227,112 - -1.6 227,152 + -0.3 227,160 - -0.7 227,160 - +1.4 227,170 + -0.2 227,175 - -1.0 227,177 + -0.3 227,177 + +0.9 227,177 + -0.2 227,185 - +0.4 227,185 - -2.7 227,185 - +0.7 227,270 + GFF221 0.21 -0.2 227,283 - GFF221 0.24 -1.2 227,283 - GFF221 0.24 -0.4 227,286 + GFF221 0.25 +0.1 227,294 - GFF221 0.27 -0.9 227,301 + GFF221 0.29 +0.4 227,326 + GFF221 0.35 -0.3 227,326 - GFF221 0.35 +1.1 227,334 - GFF221 0.36 +0.4 227,339 + GFF221 0.38 +1.0 227,339 + GFF221 0.38 -1.9 227,339 + GFF221 0.38 -0.0 227,343 + GFF221 0.38 +0.9 227,351 - GFF221 0.40 +0.4 227,351 - GFF221 0.40 -0.4 227,437 - GFF221 0.61 -0.5 227,444 + GFF221 0.62 -1.3 227,458 - GFF221 0.65 +0.0 227,474 + GFF221 0.69 +0.5 227,474 + GFF221 0.69 +0.9 227,482 - GFF221 0.71 +1.3 227,490 + GFF221 0.73 -1.5 227,503 + GFF221 0.76 -0.8 227,503 + GFF221 0.76 +0.0 227,503 + GFF221 0.76 -1.3 227,503 + GFF221 0.76 -0.3 227,503 + GFF221 0.76 +0.1 227,503 + GFF221 0.76 +0.3 227,511 - GFF221 0.78 -0.2 227,511 - GFF221 0.78 +0.9 227,517 - GFF221 0.79 -0.4 227,549 - GFF221 0.87 +0.6 227,553 - GFF221 0.88 +0.4 227,553 - GFF221 0.88 -0.8 227,558 + GFF221 0.89 -1.6 227,566 - +0.3 227,576 - -0.2 227,587 - -0.2 227,606 + -0.8 227,614 - +0.9 227,619 - -0.2 227,637 + -1.8 227,669 + -0.9 227,669 + -0.3 227,677 - +0.8 227,718 + +1.0 227,726 - -0.7 227,726 - -0.9 227,745 + -0.6 227,745 + +0.1 227,745 + +1.5 227,745 + -0.2 227,750 + +1.7 227,753 - -0.7 227,753 - -0.8 227,753 - +0.6 227,753 - -1.2 227,779 - +1.2 227,792 + +0.7 227,792 + +1.5 227,792 + +0.0 227,800 - +0.3 227,800 - +0.0 227,826 + -0.0 227,834 - +0.1 227,847 + +1.5 227,847 + -2.2 227,868 + -0.5 227,868 + +0.5 227,868 + -0.4 227,880 + +0.3 227,883 + -0.0 227,905 + GFF222 0.12 -2.0 227,907 + GFF222 0.12 -1.5 227,913 + GFF222 0.13 -1.1 227,946 + GFF222 0.16 +0.4 227,954 - GFF222 0.17 +0.2 227,976 + GFF222 0.19 +0.4 228,020 - GFF222 0.24 +0.1 228,025 + GFF222 0.24 -0.3 228,037 + GFF222 0.25 -0.6 228,047 + GFF222 0.26 -0.8 228,047 + GFF222 0.26 -0.4 228,050 - GFF222 0.27 -1.1 228,057 + GFF222 0.27 +0.5 228,073 - GFF222 0.29 -1.4 228,093 + GFF222 0.31 -0.4 228,100 - GFF222 0.32 -1.0
Or see this region's nucleotide sequence