Strain Fitness in Sphingobium sp. HT1-2 around GFF2

Experiment: EMM_noCarbon with Sucrose and Putrescine

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	EMM_noCarbon with Sucrose and Putrescine
215	-				+0.9
219	+				-0.2
219	+				+1.0
225	+				+1.3
225	-				-0.5
225	-				-1.9
225	-				+0.5
225	-				-1.0
299	+				-0.3
299	+				+0.4
299	+				+0.4
299	+				+0.3
299	+				+0.2
299	+				-0.3
299	+				-0.1
301	+				-0.2
301	+				-0.0
307	-				+0.1
307	-				-1.0
307	-				-0.4
309	-				+0.4
336	+				+0.3
338	+				-0.6
339	-				+0.3
356	-				+0.2
358	+				-0.5
363	+				+0.4
366	-				+0.4
379	+		GFF1	0.11	-0.5
397	+		GFF1	0.13	-1.6
409	-		GFF1	0.15	+0.3
468	+		GFF1	0.22	-1.2
468	+		GFF1	0.22	-0.8
468	+		GFF1	0.22	-1.6
468	+		GFF1	0.22	-0.0
468	+		GFF1	0.22	+0.9
469	+		GFF1	0.22	-0.8
470	+		GFF1	0.22	+0.4
470	-		GFF1	0.22	-1.3
476	-		GFF1	0.23	+0.2
476	-		GFF1	0.23	-0.3
477	-		GFF1	0.23	+0.8
477	-		GFF1	0.23	+0.3
480	+		GFF1	0.23	+0.4
488	-		GFF1	0.24	-1.2
519	+		GFF1	0.28	-0.2
527	-		GFF1	0.29	-0.1
527	-		GFF1	0.29	-0.0
527	-		GFF1	0.29	-1.0
527	-		GFF1	0.29	-0.9
536	+		GFF1	0.30	-1.8
627	-		GFF1	0.41	+0.5
628	+		GFF1	0.41	+0.7
628	+		GFF1	0.41	+0.3
628	+		GFF1	0.41	+0.5
656	-		GFF1	0.45	+0.2
663	+		GFF1	0.45	+0.6
663	+		GFF1	0.45	+1.2
663	+		GFF1	0.45	-0.6
663	+		GFF1	0.45	-0.3
663	+		GFF1	0.45	+0.3
671	-		GFF1	0.46	-0.6
671	-		GFF1	0.46	+0.3
671	-		GFF1	0.46	-1.5
671	-		GFF1	0.46	-1.2
697	+		GFF1	0.50	+0.4
697	+		GFF1	0.50	+0.7
697	+		GFF1	0.50	-1.2
705	-		GFF1	0.51	+0.3
705	-		GFF1	0.51	-0.2
717	-		GFF1	0.52	-1.5
719	-		GFF1	0.52	+0.5
741	+		GFF1	0.55	-0.4
743	+		GFF1	0.55	-1.6
743	+		GFF1	0.55	-0.1
751	-		GFF1	0.56	-0.6
751	-		GFF1	0.56	-0.6
751	-		GFF1	0.56	+0.2
751	-		GFF1	0.56	-0.6
751	-		GFF1	0.56	-1.2
760	-		GFF1	0.57	-0.7
767	-		GFF1	0.58	-0.4
772	+		GFF1	0.59	+0.2
772	+		GFF1	0.59	+0.2
772	+		GFF1	0.59	+1.5
780	-		GFF1	0.60	-1.4
791	-		GFF1	0.61	+0.3
808	+		GFF1	0.63	-0.7
808	+		GFF1	0.63	-0.1
808	+		GFF1	0.63	+0.0
816	-		GFF1	0.64	-0.8
833	+		GFF1	0.66	-1.7
833	+		GFF1	0.66	+0.7
833	+		GFF1	0.66	+0.5
841	-		GFF1	0.67	-0.2
857	-		GFF1	0.69	+1.0
870	-		GFF1	0.70	-2.0
870	-		GFF1	0.70	-0.5
882	+		GFF1	0.72	+0.2
882	-		GFF1	0.72	+0.6
887	+		GFF1	0.72	-1.0
890	-		GFF1	0.73	-1.7
895	-		GFF1	0.73	+0.1
895	-		GFF1	0.73	+0.6
931	+		GFF1	0.78	+1.2
931	+		GFF1	0.78	-2.8
939	-		GFF1	0.79	-0.1
947	-		GFF1	0.80	+0.4
956	+		GFF1	0.81	+1.1
956	+		GFF1	0.81	-0.8
964	-		GFF1	0.82	-1.2
964	-		GFF1	0.82	-0.3
964	-		GFF1	0.82	-0.3
964	-		GFF1	0.82	+1.4
964	-		GFF1	0.82	-0.7
966	-		GFF1	0.82	+0.2
1,003	-		GFF1	0.86	-1.6
1,055	+				-3.3
1,066	+				+1.5
1,081	-				+0.8
1,126	+				-0.2
1,126	+				+0.4
1,134	-				+0.6
1,144	+				-0.2
1,152	+				-0.2
1,152	+				-0.8
1,152	-				+0.9
1,152	-				-0.6
1,157	+				-1.4
1,162	+				-0.5
1,165	-				+1.6
1,170	-				-0.2
1,170	-				+0.3
1,192	+				+0.4
1,202	+				+0.9
1,210	-				-0.8
1,249	-				+0.8
1,249	-				+0.3
1,250	-				-1.7
1,258	-		GFF2	0.10	-1.5
1,285	+		GFF2	0.14	+1.8
1,285	+		GFF2	0.14	-0.5
1,285	+		GFF2	0.14	-0.4
1,293	+		GFF2	0.15	-0.1
1,293	-		GFF2	0.15	-0.5
1,293	-		GFF2	0.15	+0.7
1,299	-		GFF2	0.15	+0.9
1,299	-		GFF2	0.15	-0.0
1,341	+		GFF2	0.21	-0.8
1,359	+		GFF2	0.23	-1.0
1,359	+		GFF2	0.23	-0.5
1,359	+		GFF2	0.23	+0.3
1,359	+		GFF2	0.23	-1.9
1,359	+		GFF2	0.23	+0.6
1,359	+		GFF2	0.23	-1.8
1,367	-		GFF2	0.24	-0.4
1,367	-		GFF2	0.24	+0.0
1,367	-		GFF2	0.24	+0.3
1,367	-		GFF2	0.24	-0.7
1,367	-		GFF2	0.24	-0.6
1,367	-		GFF2	0.24	-0.3
1,368	+		GFF2	0.25	+0.4
1,373	+		GFF2	0.25	-1.5
1,396	+		GFF2	0.28	-0.7
1,404	-		GFF2	0.29	-0.5
1,406	+		GFF2	0.30	+0.2
1,406	+		GFF2	0.30	+1.3
1,406	+		GFF2	0.30	-2.4
1,412	-		GFF2	0.30	-0.8
1,414	-		GFF2	0.31	+0.2
1,414	-		GFF2	0.31	+0.9
1,414	-		GFF2	0.31	-1.3
1,414	-		GFF2	0.31	-2.4
1,427	-		GFF2	0.32	+1.1
1,438	-		GFF2	0.34	-1.8
1,440	-		GFF2	0.34	-0.0
1,469	-		GFF2	0.38	-0.8
1,469	-		GFF2	0.38	-0.1
1,469	-		GFF2	0.38	-1.6
1,495	-		GFF2	0.41	-1.2
1,511	+		GFF2	0.43	+0.5
1,577	+		GFF2	0.52	+0.2
1,598	+		GFF2	0.55	+0.6
1,611	-		GFF2	0.57	-0.7
1,628	-		GFF2	0.59	-0.1
1,637	-		GFF2	0.60	-0.0
1,639	-		GFF2	0.60	-1.6
1,656	+		GFF2	0.62	-0.9
1,656	+		GFF2	0.62	+0.6
1,664	-		GFF2	0.64	-0.2
1,743	+		GFF2	0.74	-0.3
1,743	+		GFF2	0.74	-0.8
1,748	+		GFF2	0.75	+0.7
1,749	+		GFF2	0.75	-0.6
1,751	-		GFF2	0.75	-0.3
1,760	+		GFF2	0.76	+0.6
1,766	-		GFF2	0.77	-0.9
1,781	+		GFF2	0.79	-2.0
1,786	+		GFF2	0.80	+0.3
1,796	+		GFF2	0.81	+0.1
1,845	-		GFF2	0.87	-0.6
1,857	+		GFF2	0.89	+0.7
1,896	+				-0.4
2,045	-				-0.1
2,063	-				-1.2
2,071	+				-1.2
2,071	+				+0.9
2,071	+				-1.2
2,071	+				-0.5
2,071	+				+0.7
2,071	+				+0.3
2,071	+				-0.2
2,071	+				+0.1
2,071	+				-0.5
2,071	+				+0.5
2,071	+				+0.4
2,071	+				-2.4
2,071	+				-1.2
2,071	+				-1.1
2,071	+				-1.5
2,071	+				-1.2
2,079	-				-1.5
2,079	-				+0.3
2,079	-				-1.1
2,079	-				-0.8
2,079	-				-2.4
2,079	-				-0.4
2,079	-				+0.1
2,079	-				-1.4
2,079	-				-1.5
2,079	-				-1.5
2,079	-				-0.4
2,079	-				-0.7
2,079	-				+0.3
2,079	-				-0.3
2,079	-				+0.4
2,079	-				-0.4
2,079	-				-0.6
2,079	-				+0.5
2,079	-				-1.6
2,079	-				+0.3
2,079	-				+0.3
2,079	-				-2.9
2,079	-				-1.0
2,079	-				-1.2
2,079	-				+1.0
2,079	-				-1.6
2,079	-				+0.6
2,079	-				+0.4
2,079	-				-1.0
2,079	-				+0.0
2,079	-				-1.0
2,079	-				+1.2
2,079	-				-0.2
2,079	-				+1.0
2,079	-				+0.6
2,079	-				-0.2
2,081	+				-2.7
2,081	-				+1.4
2,088	+				+0.4
2,113	-				-0.7
2,118	-				+0.6
2,174	-				+0.2
2,174	-				+0.7
2,177	+				-2.4
2,226	-				-1.3
2,238	-		GFF3	0.11	-0.0
2,238	-		GFF3	0.11	-0.0
2,270	-		GFF3	0.16	+0.1
2,270	-		GFF3	0.16	-1.7
2,278	+		GFF3	0.17	+0.0
2,293	+		GFF3	0.19	-0.2
2,293	+		GFF3	0.19	+0.2
2,293	+		GFF3	0.19	-1.2
2,296	-		GFF3	0.20	-0.1
2,300	+		GFF3	0.20	+0.5
2,301	+		GFF3	0.20	-1.5
2,301	+		GFF3	0.20	-0.9
2,301	+		GFF3	0.20	+0.6
2,301	-		GFF3	0.20	+0.9
2,301	-		GFF3	0.20	-0.2
2,301	-		GFF3	0.20	+0.9
2,308	-		GFF3	0.21	+0.2
2,309	-		GFF3	0.21	-1.3
2,309	-		GFF3	0.21	+0.5
2,332	+		GFF3	0.25	+0.8
2,332	+		GFF3	0.25	+0.3
2,332	+		GFF3	0.25	-1.0
2,332	+		GFF3	0.25	-1.7
2,332	+		GFF3	0.25	-0.5
2,332	+		GFF3	0.25	+1.6
2,335	-		GFF3	0.25	+0.1
2,340	-		GFF3	0.26	+0.9
2,340	-		GFF3	0.26	-0.7
2,340	-		GFF3	0.26	-1.7
2,340	-		GFF3	0.26	+0.7
2,347	-		GFF3	0.27	-1.7
2,367	+		GFF3	0.30	+0.9
2,367	+		GFF3	0.30	+0.7
2,367	+		GFF3	0.30	+1.0
2,367	+		GFF3	0.30	-0.2
2,367	+		GFF3	0.30	-0.7
2,375	-		GFF3	0.31	-0.2
2,375	-		GFF3	0.31	+0.3
2,375	-		GFF3	0.31	-0.2
2,375	-		GFF3	0.31	+0.6
2,375	-		GFF3	0.31	+0.8
2,375	-		GFF3	0.31	+1.9
2,375	-		GFF3	0.31	-3.1
2,377	+		GFF3	0.31	-0.2
2,385	-		GFF3	0.32	-2.7
2,390	+		GFF3	0.33	+0.1
2,411	-		GFF3	0.36	-0.0
2,487	+		GFF3	0.46	+0.0
2,487	-		GFF3	0.46	-2.5
2,492	+		GFF3	0.47	-0.9
2,494	+		GFF3	0.47	+0.7
2,502	-		GFF3	0.48	-0.2
2,502	-		GFF3	0.48	+0.2
2,502	-		GFF3	0.48	-0.2
2,513	-		GFF3	0.50	+0.1
2,553	+		GFF3	0.56	-0.0
2,561	-		GFF3	0.57	+1.1
2,563	+		GFF3	0.57	+0.2
2,563	+		GFF3	0.57	-0.7
2,563	+		GFF3	0.57	+0.1
2,563	+		GFF3	0.57	-0.5
2,563	+		GFF3	0.57	+0.9
2,563	+		GFF3	0.57	-0.6
2,563	+		GFF3	0.57	+0.8
2,566	-		GFF3	0.57	+0.1
2,566	-		GFF3	0.57	-0.0
2,566	-		GFF3	0.57	+0.4
2,571	-		GFF3	0.58	+0.7
2,571	-		GFF3	0.58	-0.2
2,571	-		GFF3	0.58	-1.0
2,571	-		GFF3	0.58	+0.0
2,590	-		GFF3	0.61	+1.1
2,606	-		GFF3	0.63	-0.8
2,608	+		GFF3	0.63	-1.4
2,618	+		GFF3	0.65	-1.1
2,618	+		GFF3	0.65	-1.7
2,618	+		GFF3	0.65	+1.1
2,618	+		GFF3	0.65	-0.9
2,618	+		GFF3	0.65	+0.1
2,626	+		GFF3	0.66	+0.8
2,626	-		GFF3	0.66	-1.8
2,626	-		GFF3	0.66	-0.4
2,626	-		GFF3	0.66	-0.0
2,626	-		GFF3	0.66	+0.9
2,627	+		GFF3	0.66	-0.7
2,640	+		GFF3	0.68	-0.8
2,648	-		GFF3	0.69	+0.6
2,654	+		GFF3	0.70	+0.8
2,662	+		GFF3	0.71	+0.0
2,662	+		GFF3	0.71	-1.4
2,662	+		GFF3	0.71	+0.2
2,662	-		GFF3	0.71	-2.0
2,670	-		GFF3	0.72	-0.5
2,670	-		GFF3	0.72	+0.6
2,675	-		GFF3	0.73	+0.8
2,680	-		GFF3	0.73	+0.6
2,702	+		GFF3	0.76	+0.6
2,702	+		GFF3	0.76	+0.7
2,702	+		GFF3	0.76	-0.6
2,713	+		GFF3	0.78	-1.7
2,718	+		GFF3	0.79	-1.2
2,723	+		GFF3	0.79	-0.6
2,736	-		GFF3	0.81	-0.6
2,737	+		GFF3	0.81	-0.7
2,744	+		GFF3	0.82	+0.6
2,752	-		GFF3	0.83	-1.2
2,758	+		GFF3	0.84	+0.2
2,758	+		GFF3	0.84	-1.2
2,766	-		GFF3	0.85	-0.5
2,766	-		GFF3	0.85	-0.2
2,766	-		GFF3	0.85	-1.9
2,783	+		GFF3	0.88	-0.2
2,783	+		GFF3	0.88	-0.6
2,783	+		GFF3	0.88	+1.2
2,783	+		GFF3	0.88	+0.8
2,783	+		GFF3	0.88	+1.3
2,783	+		GFF3	0.88	+0.8
2,783	+		GFF3	0.88	-1.7
2,783	+		GFF3	0.88	+0.0
2,783	+		GFF3	0.88	-0.3
2,783	+		GFF3	0.88	-0.0
2,783	+		GFF3	0.88	-1.1
2,783	+		GFF3	0.88	-1.3
2,783	+		GFF3	0.88	+0.2
2,783	+		GFF3	0.88	-0.5
2,791	+		GFF3	0.89	-2.0
2,791	-		GFF3	0.89	-1.6
2,791	-		GFF3	0.89	+0.4
2,791	-		GFF3	0.89	-0.1
2,791	-		GFF3	0.89	+0.2
2,791	-		GFF3	0.89	+0.1
2,791	-		GFF3	0.89	+0.5
2,791	-		GFF3	0.89	-0.6
2,801	+				+1.0
2,810	+				-1.5
2,845	-				-0.2
2,894	+				-0.9
2,895	+				+0.8
2,900	+				-1.6
2,900	-				-1.2
2,902	+				+0.1
2,902	-				-1.8
2,918	+				-2.0
2,931	-				+0.4
2,931	-				-1.1
2,937	+				+0.4