Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1888 and GFF1889 are separated by 54 nucleotides GFF1889 and GFF1890 are separated by 30 nucleotides GFF1890 and GFF1891 are separated by 132 nucleotides
GFF1888 - Acetyltransferase, GNAT family, at 1,912,851 to 1,913,378
GFF1888
GFF1889 - Transcriptional regulator, AcrR family, at 1,913,433 to 1,914,113
GFF1889
GFF1890 - GTP-binding protein Obg, at 1,914,144 to 1,915,238
GFF1890
GFF1891 - Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA, at 1,915,371 to 1,916,504
GFF1891
Position (kb)
1914
1915
1916 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1913.183 kb on + strand, within GFF1888 at 1913.191 kb on - strand, within GFF1888 at 1913.205 kb on + strand, within GFF1888 at 1913.206 kb on - strand, within GFF1888 at 1913.207 kb on + strand, within GFF1888 at 1913.213 kb on - strand, within GFF1888 at 1913.215 kb on - strand, within GFF1888 at 1913.217 kb on + strand, within GFF1888 at 1913.229 kb on + strand, within GFF1888 at 1913.237 kb on - strand, within GFF1888 at 1913.279 kb on + strand, within GFF1888 at 1913.280 kb on + strand, within GFF1888 at 1913.289 kb on + strand, within GFF1888 at 1913.293 kb on - strand, within GFF1888 at 1913.311 kb on + strand, within GFF1888 at 1913.311 kb on + strand, within GFF1888 at 1913.319 kb on - strand, within GFF1888 at 1913.319 kb on - strand, within GFF1888 at 1913.339 kb on + strand at 1913.339 kb on + strand at 1913.339 kb on + strand at 1913.339 kb on + strand at 1913.339 kb on + strand at 1913.347 kb on - strand at 1913.361 kb on + strand at 1913.363 kb on - strand at 1913.366 kb on + strand at 1913.366 kb on + strand at 1913.366 kb on + strand at 1913.369 kb on - strand at 1913.374 kb on - strand at 1913.374 kb on - strand at 1913.374 kb on - strand at 1913.382 kb on - strand at 1913.392 kb on - strand at 1913.399 kb on + strand at 1913.409 kb on + strand at 1913.417 kb on - strand at 1913.426 kb on - strand at 1913.426 kb on - strand at 1913.426 kb on - strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.430 kb on + strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.438 kb on - strand at 1913.444 kb on - strand at 1913.455 kb on + strand at 1913.455 kb on + strand at 1913.455 kb on + strand at 1913.455 kb on + strand at 1913.458 kb on - strand at 1913.487 kb on + strand at 1913.489 kb on + strand at 1913.504 kb on + strand, within GFF1889 at 1913.510 kb on - strand, within GFF1889 at 1913.514 kb on + strand, within GFF1889 at 1913.545 kb on + strand, within GFF1889 at 1913.566 kb on - strand, within GFF1889 at 1913.569 kb on + strand, within GFF1889 at 1913.570 kb on + strand, within GFF1889 at 1913.578 kb on + strand, within GFF1889 at 1913.611 kb on + strand, within GFF1889 at 1913.619 kb on - strand, within GFF1889 at 1913.620 kb on + strand, within GFF1889 at 1913.628 kb on - strand, within GFF1889 at 1913.723 kb on - strand, within GFF1889 at 1913.725 kb on - strand, within GFF1889 at 1913.730 kb on - strand, within GFF1889 at 1913.735 kb on - strand, within GFF1889 at 1913.756 kb on - strand, within GFF1889 at 1913.770 kb on + strand, within GFF1889 at 1913.802 kb on + strand, within GFF1889 at 1913.807 kb on + strand, within GFF1889 at 1913.807 kb on + strand, within GFF1889 at 1913.807 kb on + strand, within GFF1889 at 1913.807 kb on + strand, within GFF1889 at 1913.807 kb on + strand, within GFF1889 at 1913.812 kb on + strand, within GFF1889 at 1913.815 kb on - strand, within GFF1889 at 1913.820 kb on - strand, within GFF1889 at 1913.820 kb on - strand, within GFF1889 at 1913.835 kb on + strand, within GFF1889 at 1913.856 kb on - strand, within GFF1889 at 1913.877 kb on + strand, within GFF1889 at 1913.901 kb on - strand, within GFF1889 at 1913.915 kb on + strand, within GFF1889 at 1913.915 kb on + strand, within GFF1889 at 1913.916 kb on + strand, within GFF1889 at 1913.923 kb on - strand, within GFF1889 at 1913.933 kb on - strand, within GFF1889 at 1913.944 kb on + strand, within GFF1889 at 1913.948 kb on + strand, within GFF1889 at 1913.948 kb on + strand, within GFF1889 at 1913.950 kb on - strand, within GFF1889 at 1913.992 kb on + strand, within GFF1889 at 1913.992 kb on + strand, within GFF1889 at 1913.992 kb on + strand, within GFF1889 at 1913.997 kb on + strand, within GFF1889 at 1914.000 kb on - strand, within GFF1889 at 1914.000 kb on - strand, within GFF1889 at 1914.000 kb on - strand, within GFF1889 at 1914.000 kb on - strand, within GFF1889 at 1914.000 kb on - strand, within GFF1889 at 1914.047 kb on + strand at 1914.059 kb on + strand at 1914.059 kb on + strand at 1914.059 kb on + strand at 1915.148 kb on + strand at 1915.148 kb on + strand at 1915.148 kb on + strand at 1915.148 kb on + strand at 1915.156 kb on - strand at 1915.156 kb on - strand at 1915.156 kb on - strand at 1915.198 kb on - strand at 1915.258 kb on - strand at 1915.259 kb on - strand at 1915.273 kb on - strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.275 kb on + strand at 1915.283 kb on - strand at 1915.283 kb on - strand at 1915.283 kb on - strand at 1915.283 kb on - strand at 1915.283 kb on - strand at 1915.283 kb on - strand at 1915.294 kb on + strand at 1915.294 kb on + strand at 1915.294 kb on + strand at 1915.302 kb on - strand at 1915.302 kb on - strand at 1915.305 kb on + strand at 1915.310 kb on + strand at 1915.310 kb on + strand at 1915.310 kb on + strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.318 kb on - strand at 1915.341 kb on + strand at 1915.341 kb on + strand at 1915.349 kb on - strand at 1915.354 kb on - strand at 1915.383 kb on + strand at 1915.393 kb on + strand at 1915.393 kb on + strand at 1915.399 kb on + strand at 1915.399 kb on + strand at 1915.404 kb on + strand at 1915.404 kb on + strand at 1915.412 kb on - strand at 1915.412 kb on - strand at 1915.425 kb on - strand at 1915.484 kb on + strand at 1915.533 kb on - strand, within GFF1891 at 1915.540 kb on - strand, within GFF1891 at 1915.576 kb on + strand, within GFF1891 at 1915.584 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.586 kb on + strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.594 kb on - strand, within GFF1891 at 1915.671 kb on - strand, within GFF1891 at 1915.685 kb on + strand, within GFF1891 at 1915.685 kb on + strand, within GFF1891 at 1915.709 kb on + strand, within GFF1891 at 1915.723 kb on + strand, within GFF1891 at 1915.731 kb on - strand, within GFF1891 at 1915.754 kb on - strand, within GFF1891 at 1915.757 kb on + strand, within GFF1891 at 1915.762 kb on + strand, within GFF1891 at 1915.814 kb on - strand, within GFF1891 at 1915.835 kb on - strand, within GFF1891 at 1915.894 kb on + strand, within GFF1891 at 1915.894 kb on + strand, within GFF1891 at 1915.902 kb on - strand, within GFF1891 at 1915.904 kb on + strand, within GFF1891 at 1915.904 kb on - strand, within GFF1891 at 1915.904 kb on - strand, within GFF1891 at 1915.904 kb on - strand, within GFF1891 at 1915.912 kb on - strand, within GFF1891 at 1915.914 kb on - strand, within GFF1891 at 1915.914 kb on - strand, within GFF1891 at 1915.921 kb on + strand, within GFF1891 at 1915.922 kb on + strand, within GFF1891 at 1915.922 kb on + strand, within GFF1891 at 1915.922 kb on + strand, within GFF1891 at 1915.922 kb on + strand, within GFF1891 at 1915.930 kb on - strand, within GFF1891 at 1915.930 kb on - strand, within GFF1891 at 1915.937 kb on + strand, within GFF1891 at 1915.938 kb on - strand, within GFF1891 at 1915.945 kb on - strand, within GFF1891 at 1915.962 kb on - strand, within GFF1891 at 1915.964 kb on + strand, within GFF1891 at 1915.964 kb on - strand, within GFF1891 at 1915.969 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.972 kb on + strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1915.980 kb on - strand, within GFF1891 at 1916.006 kb on + strand, within GFF1891 at 1916.014 kb on - strand, within GFF1891 at 1916.030 kb on - strand, within GFF1891 at 1916.034 kb on + strand, within GFF1891 at 1916.034 kb on + strand, within GFF1891 at 1916.044 kb on + strand, within GFF1891 at 1916.062 kb on + strand, within GFF1891 at 1916.065 kb on - strand, within GFF1891 at 1916.067 kb on + strand, within GFF1891 at 1916.075 kb on - strand, within GFF1891 at 1916.075 kb on - strand, within GFF1891 at 1916.117 kb on + strand, within GFF1891 at 1916.117 kb on + strand, within GFF1891 at 1916.117 kb on + strand, within GFF1891 at 1916.201 kb on - strand, within GFF1891 at 1916.207 kb on - strand, within GFF1891 at 1916.215 kb on + strand, within GFF1891 at 1916.219 kb on - strand, within GFF1891
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,913,183 + GFF1888 0.63 -0.4 1,913,191 - GFF1888 0.64 +1.8 1,913,205 + GFF1888 0.67 -0.3 1,913,206 - GFF1888 0.67 -2.0 1,913,207 + GFF1888 0.67 -0.6 1,913,213 - GFF1888 0.69 +0.3 1,913,215 - GFF1888 0.69 -0.3 1,913,217 + GFF1888 0.69 -0.5 1,913,229 + GFF1888 0.72 +1.2 1,913,237 - GFF1888 0.73 +2.2 1,913,279 + GFF1888 0.81 +0.1 1,913,280 + GFF1888 0.81 -0.8 1,913,289 + GFF1888 0.83 -0.3 1,913,293 - GFF1888 0.84 +0.9 1,913,311 + GFF1888 0.87 -1.8 1,913,311 + GFF1888 0.87 -0.5 1,913,319 - GFF1888 0.89 +1.2 1,913,319 - GFF1888 0.89 -0.6 1,913,339 + +0.6 1,913,339 + -0.8 1,913,339 + +0.1 1,913,339 + -0.1 1,913,339 + -0.1 1,913,347 - -1.0 1,913,361 + -0.1 1,913,363 - +1.5 1,913,366 + -1.6 1,913,366 + -1.1 1,913,366 + -1.9 1,913,369 - -1.1 1,913,374 - -0.9 1,913,374 - -0.5 1,913,374 - -0.5 1,913,382 - +0.2 1,913,392 - +0.1 1,913,399 + +2.0 1,913,409 + -0.6 1,913,417 - +0.5 1,913,426 - +0.9 1,913,426 - -1.3 1,913,426 - -1.1 1,913,430 + -1.5 1,913,430 + +1.4 1,913,430 + +0.2 1,913,430 + +0.7 1,913,430 + -0.3 1,913,430 + +0.5 1,913,430 + -0.7 1,913,430 + +0.4 1,913,430 + -1.1 1,913,438 - -1.4 1,913,438 - +1.2 1,913,438 - +0.3 1,913,438 - +0.0 1,913,438 - +1.1 1,913,438 - -0.3 1,913,438 - -0.5 1,913,438 - -1.2 1,913,444 - -1.1 1,913,455 + -2.1 1,913,455 + -0.0 1,913,455 + -0.9 1,913,455 + +0.2 1,913,458 - -1.7 1,913,487 + +0.7 1,913,489 + -0.3 1,913,504 + GFF1889 0.10 -0.0 1,913,510 - GFF1889 0.11 -1.9 1,913,514 + GFF1889 0.12 +1.3 1,913,545 + GFF1889 0.16 +0.4 1,913,566 - GFF1889 0.20 -0.6 1,913,569 + GFF1889 0.20 +0.1 1,913,570 + GFF1889 0.20 -1.0 1,913,578 + GFF1889 0.21 -0.3 1,913,611 + GFF1889 0.26 +0.9 1,913,619 - GFF1889 0.27 -0.9 1,913,620 + GFF1889 0.27 +0.9 1,913,628 - GFF1889 0.29 +0.3 1,913,723 - GFF1889 0.43 -1.9 1,913,725 - GFF1889 0.43 -0.5 1,913,730 - GFF1889 0.44 -0.9 1,913,735 - GFF1889 0.44 +0.8 1,913,756 - GFF1889 0.47 +1.5 1,913,770 + GFF1889 0.49 -0.6 1,913,802 + GFF1889 0.54 -0.9 1,913,807 + GFF1889 0.55 -0.2 1,913,807 + GFF1889 0.55 +0.3 1,913,807 + GFF1889 0.55 -1.1 1,913,807 + GFF1889 0.55 -1.3 1,913,807 + GFF1889 0.55 +0.0 1,913,812 + GFF1889 0.56 +0.7 1,913,815 - GFF1889 0.56 +0.6 1,913,820 - GFF1889 0.57 -0.9 1,913,820 - GFF1889 0.57 +0.3 1,913,835 + GFF1889 0.59 +0.3 1,913,856 - GFF1889 0.62 -1.7 1,913,877 + GFF1889 0.65 -0.1 1,913,901 - GFF1889 0.69 -0.8 1,913,915 + GFF1889 0.71 -0.6 1,913,915 + GFF1889 0.71 -1.1 1,913,916 + GFF1889 0.71 +0.0 1,913,923 - GFF1889 0.72 -0.7 1,913,933 - GFF1889 0.73 -0.5 1,913,944 + GFF1889 0.75 -0.2 1,913,948 + GFF1889 0.76 -0.0 1,913,948 + GFF1889 0.76 -0.3 1,913,950 - GFF1889 0.76 +0.2 1,913,992 + GFF1889 0.82 -0.6 1,913,992 + GFF1889 0.82 -0.1 1,913,992 + GFF1889 0.82 +1.2 1,913,997 + GFF1889 0.83 +0.2 1,914,000 - GFF1889 0.83 -1.4 1,914,000 - GFF1889 0.83 +0.3 1,914,000 - GFF1889 0.83 +0.0 1,914,000 - GFF1889 0.83 +1.7 1,914,000 - GFF1889 0.83 -0.9 1,914,047 + -1.3 1,914,059 + -0.0 1,914,059 + +0.7 1,914,059 + +1.8 1,915,148 + -0.3 1,915,148 + -0.8 1,915,148 + -1.5 1,915,148 + -0.0 1,915,156 - +1.1 1,915,156 - +0.4 1,915,156 - +0.2 1,915,198 - -1.3 1,915,258 - -1.1 1,915,259 - -0.3 1,915,273 - -2.6 1,915,275 + -0.0 1,915,275 + -2.0 1,915,275 + -0.4 1,915,275 + -1.8 1,915,275 + -1.0 1,915,275 + +1.1 1,915,275 + +0.1 1,915,275 + -0.6 1,915,283 - -0.3 1,915,283 - -0.3 1,915,283 - +0.2 1,915,283 - +0.1 1,915,283 - -2.7 1,915,283 - -1.3 1,915,294 + -1.1 1,915,294 + -0.7 1,915,294 + +0.5 1,915,302 - -0.1 1,915,302 - -1.7 1,915,305 + +0.3 1,915,310 + +0.8 1,915,310 + -0.4 1,915,310 + +0.2 1,915,318 - +0.7 1,915,318 - -0.3 1,915,318 - -1.5 1,915,318 - +0.5 1,915,318 - +0.5 1,915,318 - -0.7 1,915,318 - -1.2 1,915,318 - -1.3 1,915,341 + +0.9 1,915,341 + +0.9 1,915,349 - -2.4 1,915,354 - +0.7 1,915,383 + +1.2 1,915,393 + -1.3 1,915,393 + -1.3 1,915,399 + -1.8 1,915,399 + -1.5 1,915,404 + -2.1 1,915,404 + -1.5 1,915,412 - -3.4 1,915,412 - -2.2 1,915,425 - -0.4 1,915,484 + -2.6 1,915,533 - GFF1891 0.14 -0.8 1,915,540 - GFF1891 0.15 -2.2 1,915,576 + GFF1891 0.18 -2.4 1,915,584 + GFF1891 0.19 -1.6 1,915,586 + GFF1891 0.19 -0.9 1,915,586 + GFF1891 0.19 -1.1 1,915,586 + GFF1891 0.19 -1.6 1,915,586 + GFF1891 0.19 -0.1 1,915,586 + GFF1891 0.19 -0.3 1,915,586 + GFF1891 0.19 -1.8 1,915,586 + GFF1891 0.19 -1.3 1,915,586 + GFF1891 0.19 -2.6 1,915,594 - GFF1891 0.20 -2.3 1,915,594 - GFF1891 0.20 -1.9 1,915,594 - GFF1891 0.20 -2.6 1,915,594 - GFF1891 0.20 -2.7 1,915,671 - GFF1891 0.26 -1.8 1,915,685 + GFF1891 0.28 -1.3 1,915,685 + GFF1891 0.28 -1.8 1,915,709 + GFF1891 0.30 -0.1 1,915,723 + GFF1891 0.31 -0.8 1,915,731 - GFF1891 0.32 -1.3 1,915,754 - GFF1891 0.34 -1.7 1,915,757 + GFF1891 0.34 -2.3 1,915,762 + GFF1891 0.34 -1.5 1,915,814 - GFF1891 0.39 -0.1 1,915,835 - GFF1891 0.41 -2.1 1,915,894 + GFF1891 0.46 -0.6 1,915,894 + GFF1891 0.46 -1.2 1,915,902 - GFF1891 0.47 -1.2 1,915,904 + GFF1891 0.47 -1.9 1,915,904 - GFF1891 0.47 -1.2 1,915,904 - GFF1891 0.47 -3.5 1,915,904 - GFF1891 0.47 +0.2 1,915,912 - GFF1891 0.48 -3.0 1,915,914 - GFF1891 0.48 -0.9 1,915,914 - GFF1891 0.48 -1.8 1,915,921 + GFF1891 0.49 -1.3 1,915,922 + GFF1891 0.49 -2.0 1,915,922 + GFF1891 0.49 -0.6 1,915,922 + GFF1891 0.49 -1.8 1,915,922 + GFF1891 0.49 -2.5 1,915,930 - GFF1891 0.49 -0.4 1,915,930 - GFF1891 0.49 -1.8 1,915,937 + GFF1891 0.50 -2.9 1,915,938 - GFF1891 0.50 -0.9 1,915,945 - GFF1891 0.51 -1.7 1,915,962 - GFF1891 0.52 -1.5 1,915,964 + GFF1891 0.52 -1.1 1,915,964 - GFF1891 0.52 -3.6 1,915,969 + GFF1891 0.53 -2.6 1,915,972 + GFF1891 0.53 -1.6 1,915,972 + GFF1891 0.53 -1.4 1,915,972 + GFF1891 0.53 -0.6 1,915,972 + GFF1891 0.53 -3.2 1,915,980 - GFF1891 0.54 -3.5 1,915,980 - GFF1891 0.54 -1.5 1,915,980 - GFF1891 0.54 -2.1 1,915,980 - GFF1891 0.54 -1.3 1,916,006 + GFF1891 0.56 -2.8 1,916,014 - GFF1891 0.57 -3.0 1,916,030 - GFF1891 0.58 -3.3 1,916,034 + GFF1891 0.58 -1.9 1,916,034 + GFF1891 0.58 -3.5 1,916,044 + GFF1891 0.59 -0.5 1,916,062 + GFF1891 0.61 -2.6 1,916,065 - GFF1891 0.61 -2.6 1,916,067 + GFF1891 0.61 -2.7 1,916,075 - GFF1891 0.62 -1.3 1,916,075 - GFF1891 0.62 +0.1 1,916,117 + GFF1891 0.66 -1.3 1,916,117 + GFF1891 0.66 -1.3 1,916,117 + GFF1891 0.66 -1.3 1,916,201 - GFF1891 0.73 -1.3 1,916,207 - GFF1891 0.74 -1.5 1,916,215 + GFF1891 0.74 -2.1 1,916,219 - GFF1891 0.75 -3.2
Or see this region's nucleotide sequence