Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1626 and GFF1627 are separated by 2 nucleotides GFF1627 and GFF1628 overlap by 4 nucleotides GFF1628 and GFF1629 are separated by 33 nucleotides
GFF1626 - Tryptophan synthase beta chain (EC 4.2.1.20), at 1,654,526 to 1,655,779
GFF1626
GFF1627 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 1,655,782 to 1,656,600
GFF1627
GFF1628 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 1,656,597 to 1,657,445
GFF1628
GFF1629 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 1,657,479 to 1,658,813
GFF1629
Position (kb)
1655
1656
1657 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1654.934 kb on + strand, within GFF1626 at 1654.972 kb on + strand, within GFF1626 at 1655.119 kb on + strand, within GFF1626 at 1655.124 kb on + strand, within GFF1626 at 1655.227 kb on + strand, within GFF1626 at 1655.317 kb on + strand, within GFF1626 at 1655.317 kb on + strand, within GFF1626 at 1655.443 kb on + strand, within GFF1626 at 1655.452 kb on + strand, within GFF1626 at 1655.452 kb on + strand, within GFF1626 at 1655.468 kb on + strand, within GFF1626 at 1655.501 kb on + strand, within GFF1626 at 1655.501 kb on + strand, within GFF1626 at 1655.506 kb on + strand, within GFF1626 at 1655.523 kb on + strand, within GFF1626 at 1655.528 kb on + strand, within GFF1626 at 1655.617 kb on + strand, within GFF1626 at 1655.742 kb on + strand at 1655.778 kb on + strand at 1655.778 kb on + strand at 1655.778 kb on + strand at 1655.794 kb on + strand at 1655.826 kb on + strand at 1655.863 kb on + strand at 1655.892 kb on + strand, within GFF1627 at 1655.996 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.135 kb on + strand, within GFF1627 at 1656.143 kb on - strand, within GFF1627 at 1656.157 kb on + strand, within GFF1627 at 1656.157 kb on + strand, within GFF1627 at 1656.157 kb on + strand, within GFF1627 at 1656.203 kb on + strand, within GFF1627 at 1656.270 kb on + strand, within GFF1627 at 1656.349 kb on + strand, within GFF1627 at 1656.354 kb on + strand, within GFF1627 at 1656.381 kb on + strand, within GFF1627 at 1656.381 kb on + strand, within GFF1627 at 1656.387 kb on + strand, within GFF1627 at 1656.392 kb on + strand, within GFF1627 at 1656.439 kb on - strand, within GFF1627 at 1656.477 kb on + strand, within GFF1627 at 1656.482 kb on + strand, within GFF1627 at 1656.535 kb on + strand at 1656.565 kb on + strand at 1657.420 kb on + strand at 1657.420 kb on + strand at 1657.517 kb on + strand at 1657.532 kb on + strand at 1657.540 kb on - strand at 1657.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 1,654,934 + GFF1626 0.33 -1.9 1,654,972 + GFF1626 0.36 -1.5 1,655,119 + GFF1626 0.47 -1.6 1,655,124 + GFF1626 0.48 -1.5 1,655,227 + GFF1626 0.56 -1.7 1,655,317 + GFF1626 0.63 -1.0 1,655,317 + GFF1626 0.63 -1.2 1,655,443 + GFF1626 0.73 -1.7 1,655,452 + GFF1626 0.74 -3.1 1,655,452 + GFF1626 0.74 -1.3 1,655,468 + GFF1626 0.75 -1.3 1,655,501 + GFF1626 0.78 -1.8 1,655,501 + GFF1626 0.78 -1.8 1,655,506 + GFF1626 0.78 -1.7 1,655,523 + GFF1626 0.80 -1.2 1,655,528 + GFF1626 0.80 -1.2 1,655,617 + GFF1626 0.87 -1.6 1,655,742 + -2.2 1,655,778 + -1.5 1,655,778 + -1.2 1,655,778 + +0.9 1,655,794 + -1.3 1,655,826 + +0.1 1,655,863 + -2.2 1,655,892 + GFF1627 0.13 -2.3 1,655,996 + GFF1627 0.26 -1.8 1,656,135 + GFF1627 0.43 -1.3 1,656,135 + GFF1627 0.43 -1.5 1,656,135 + GFF1627 0.43 -1.1 1,656,135 + GFF1627 0.43 -1.5 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -1.5 1,656,135 + GFF1627 0.43 -2.6 1,656,135 + GFF1627 0.43 -3.1 1,656,135 + GFF1627 0.43 -1.6 1,656,135 + GFF1627 0.43 -1.2 1,656,135 + GFF1627 0.43 -1.9 1,656,135 + GFF1627 0.43 -2.3 1,656,135 + GFF1627 0.43 -1.9 1,656,135 + GFF1627 0.43 -0.5 1,656,135 + GFF1627 0.43 -1.6 1,656,135 + GFF1627 0.43 -1.3 1,656,135 + GFF1627 0.43 -0.7 1,656,135 + GFF1627 0.43 +0.4 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -2.0 1,656,135 + GFF1627 0.43 -1.8 1,656,135 + GFF1627 0.43 -0.4 1,656,135 + GFF1627 0.43 -1.3 1,656,135 + GFF1627 0.43 -0.7 1,656,135 + GFF1627 0.43 -0.3 1,656,135 + GFF1627 0.43 -1.3 1,656,135 + GFF1627 0.43 -0.9 1,656,135 + GFF1627 0.43 -2.7 1,656,135 + GFF1627 0.43 -1.2 1,656,135 + GFF1627 0.43 -0.8 1,656,135 + GFF1627 0.43 -1.2 1,656,143 - GFF1627 0.44 -1.2 1,656,157 + GFF1627 0.46 -1.2 1,656,157 + GFF1627 0.46 -2.2 1,656,157 + GFF1627 0.46 -1.8 1,656,203 + GFF1627 0.51 -2.6 1,656,270 + GFF1627 0.60 -1.5 1,656,349 + GFF1627 0.69 -0.3 1,656,354 + GFF1627 0.70 -0.6 1,656,381 + GFF1627 0.73 -2.5 1,656,381 + GFF1627 0.73 -0.3 1,656,387 + GFF1627 0.74 -2.5 1,656,392 + GFF1627 0.74 -0.3 1,656,439 - GFF1627 0.80 -1.8 1,656,477 + GFF1627 0.85 +1.0 1,656,482 + GFF1627 0.85 -1.5 1,656,535 + -2.1 1,656,565 + -0.2 1,657,420 + +0.3 1,657,420 + +0.6 1,657,517 + +0.5 1,657,532 + +0.6 1,657,540 - -0.8 1,657,552 - +0.2
Or see this region's nucleotide sequence