Strain Fitness in Sphingobium sp. HT1-2 around GFF1627

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1626 and GFF1627 are separated by 2 nucleotidesGFF1627 and GFF1628 overlap by 4 nucleotidesGFF1628 and GFF1629 are separated by 33 nucleotides GFF1626 - Tryptophan synthase beta chain (EC 4.2.1.20), at 1,654,526 to 1,655,779 GFF1626 GFF1627 - Tryptophan synthase alpha chain (EC 4.2.1.20), at 1,655,782 to 1,656,600 GFF1627 GFF1628 - Acetyl-coenzyme A carboxyl transferase beta chain (EC 6.4.1.2), at 1,656,597 to 1,657,445 GFF1628 GFF1629 - Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17), at 1,657,479 to 1,658,813 GFF1629 Position (kb) 1655 1656 1657Strain fitness (log2 ratio) -3 -2 -1 0 1at 1654.934 kb on + strand, within GFF1626at 1654.972 kb on + strand, within GFF1626at 1655.119 kb on + strand, within GFF1626at 1655.124 kb on + strand, within GFF1626at 1655.227 kb on + strand, within GFF1626at 1655.317 kb on + strand, within GFF1626at 1655.317 kb on + strand, within GFF1626at 1655.443 kb on + strand, within GFF1626at 1655.452 kb on + strand, within GFF1626at 1655.452 kb on + strand, within GFF1626at 1655.468 kb on + strand, within GFF1626at 1655.501 kb on + strand, within GFF1626at 1655.501 kb on + strand, within GFF1626at 1655.506 kb on + strand, within GFF1626at 1655.523 kb on + strand, within GFF1626at 1655.528 kb on + strand, within GFF1626at 1655.617 kb on + strand, within GFF1626at 1655.742 kb on + strandat 1655.778 kb on + strandat 1655.778 kb on + strandat 1655.778 kb on + strandat 1655.794 kb on + strandat 1655.826 kb on + strandat 1655.863 kb on + strandat 1655.892 kb on + strand, within GFF1627at 1655.996 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.135 kb on + strand, within GFF1627at 1656.143 kb on - strand, within GFF1627at 1656.157 kb on + strand, within GFF1627at 1656.157 kb on + strand, within GFF1627at 1656.157 kb on + strand, within GFF1627at 1656.203 kb on + strand, within GFF1627at 1656.270 kb on + strand, within GFF1627at 1656.349 kb on + strand, within GFF1627at 1656.354 kb on + strand, within GFF1627at 1656.381 kb on + strand, within GFF1627at 1656.381 kb on + strand, within GFF1627at 1656.387 kb on + strand, within GFF1627at 1656.392 kb on + strand, within GFF1627at 1656.439 kb on - strand, within GFF1627at 1656.477 kb on + strand, within GFF1627at 1656.482 kb on + strand, within GFF1627at 1656.535 kb on + strandat 1656.565 kb on + strandat 1657.420 kb on + strandat 1657.420 kb on + strandat 1657.517 kb on + strandat 1657.532 kb on + strandat 1657.540 kb on - strandat 1657.552 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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1,654,934 + GFF1626 0.33 -1.9
1,654,972 + GFF1626 0.36 -1.5
1,655,119 + GFF1626 0.47 -1.6
1,655,124 + GFF1626 0.48 -1.5
1,655,227 + GFF1626 0.56 -1.7
1,655,317 + GFF1626 0.63 -1.0
1,655,317 + GFF1626 0.63 -1.2
1,655,443 + GFF1626 0.73 -1.7
1,655,452 + GFF1626 0.74 -3.1
1,655,452 + GFF1626 0.74 -1.3
1,655,468 + GFF1626 0.75 -1.3
1,655,501 + GFF1626 0.78 -1.8
1,655,501 + GFF1626 0.78 -1.8
1,655,506 + GFF1626 0.78 -1.7
1,655,523 + GFF1626 0.80 -1.2
1,655,528 + GFF1626 0.80 -1.2
1,655,617 + GFF1626 0.87 -1.6
1,655,742 + -2.2
1,655,778 + -1.5
1,655,778 + -1.2
1,655,778 + +0.9
1,655,794 + -1.3
1,655,826 + +0.1
1,655,863 + -2.2
1,655,892 + GFF1627 0.13 -2.3
1,655,996 + GFF1627 0.26 -1.8
1,656,135 + GFF1627 0.43 -1.3
1,656,135 + GFF1627 0.43 -1.5
1,656,135 + GFF1627 0.43 -1.1
1,656,135 + GFF1627 0.43 -1.5
1,656,135 + GFF1627 0.43 -0.5
1,656,135 + GFF1627 0.43 -1.8
1,656,135 + GFF1627 0.43 -1.5
1,656,135 + GFF1627 0.43 -2.6
1,656,135 + GFF1627 0.43 -3.1
1,656,135 + GFF1627 0.43 -1.6
1,656,135 + GFF1627 0.43 -1.2
1,656,135 + GFF1627 0.43 -1.9
1,656,135 + GFF1627 0.43 -2.3
1,656,135 + GFF1627 0.43 -1.9
1,656,135 + GFF1627 0.43 -0.5
1,656,135 + GFF1627 0.43 -1.6
1,656,135 + GFF1627 0.43 -1.3
1,656,135 + GFF1627 0.43 -0.7
1,656,135 + GFF1627 0.43 +0.4
1,656,135 + GFF1627 0.43 -1.8
1,656,135 + GFF1627 0.43 -2.0
1,656,135 + GFF1627 0.43 -1.8
1,656,135 + GFF1627 0.43 -0.4
1,656,135 + GFF1627 0.43 -1.3
1,656,135 + GFF1627 0.43 -0.7
1,656,135 + GFF1627 0.43 -0.3
1,656,135 + GFF1627 0.43 -1.3
1,656,135 + GFF1627 0.43 -0.9
1,656,135 + GFF1627 0.43 -2.7
1,656,135 + GFF1627 0.43 -1.2
1,656,135 + GFF1627 0.43 -0.8
1,656,135 + GFF1627 0.43 -1.2
1,656,143 - GFF1627 0.44 -1.2
1,656,157 + GFF1627 0.46 -1.2
1,656,157 + GFF1627 0.46 -2.2
1,656,157 + GFF1627 0.46 -1.8
1,656,203 + GFF1627 0.51 -2.6
1,656,270 + GFF1627 0.60 -1.5
1,656,349 + GFF1627 0.69 -0.3
1,656,354 + GFF1627 0.70 -0.6
1,656,381 + GFF1627 0.73 -2.5
1,656,381 + GFF1627 0.73 -0.3
1,656,387 + GFF1627 0.74 -2.5
1,656,392 + GFF1627 0.74 -0.3
1,656,439 - GFF1627 0.80 -1.8
1,656,477 + GFF1627 0.85 +1.0
1,656,482 + GFF1627 0.85 -1.5
1,656,535 + -2.1
1,656,565 + -0.2
1,657,420 + +0.3
1,657,420 + +0.6
1,657,517 + +0.5
1,657,532 + +0.6
1,657,540 - -0.8
1,657,552 - +0.2

Or see this region's nucleotide sequence