Strain Fitness in Sphingobium sp. HT1-2 around GFF1452

Experiment: EMM_noCarbon with Sucrose and Putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1450 and GFF1451 overlap by 4 nucleotidesGFF1451 and GFF1452 are separated by 1 nucleotidesGFF1452 and GFF1453 overlap by 8 nucleotidesGFF1453 and GFF1454 are separated by 34 nucleotides GFF1450 - Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 1,477,942 to 1,478,730 GFF1450 GFF1451 - Conserved hypothetical protein probably involved in sulfate reduction, at 1,478,727 to 1,479,023 GFF1451 GFF1452 - Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2), at 1,479,025 to 1,480,656 GFF1452 GFF1453 - Oxidoreductase probably involved in sulfite reduction, at 1,480,649 to 1,481,089 GFF1453 GFF1454 - Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8), at 1,481,124 to 1,481,882 GFF1454 Position (kb) 1479 1480 1481Strain fitness (log2 ratio) -3 -2 -1 0 1at 1478.656 kb on + strandat 1478.656 kb on + strandat 1478.657 kb on + strandat 1478.664 kb on - strandat 1478.685 kb on - strandat 1478.950 kb on + strand, within GFF1451at 1478.950 kb on + strand, within GFF1451at 1478.950 kb on + strand, within GFF1451at 1478.958 kb on - strand, within GFF1451at 1478.968 kb on + strand, within GFF1451at 1478.973 kb on + strand, within GFF1451at 1478.976 kb on - strand, within GFF1451at 1479.003 kb on + strandat 1479.025 kb on - strandat 1479.041 kb on + strandat 1479.049 kb on - strandat 1479.113 kb on + strandat 1479.176 kb on + strandat 1479.176 kb on + strandat 1479.184 kb on - strandat 1479.378 kb on - strand, within GFF1452at 1479.412 kb on - strand, within GFF1452at 1480.104 kb on - strand, within GFF1452at 1480.647 kb on + strandat 1480.726 kb on + strand, within GFF1453at 1480.734 kb on - strand, within GFF1453at 1481.026 kb on + strand, within GFF1453at 1481.030 kb on - strand, within GFF1453at 1481.053 kb on - strandat 1481.055 kb on + strandat 1481.121 kb on + strandat 1481.142 kb on + strandat 1481.190 kb on + strandat 1481.215 kb on - strand, within GFF1454at 1481.215 kb on - strand, within GFF1454at 1481.215 kb on - strand, within GFF1454at 1481.227 kb on - strand, within GFF1454at 1481.273 kb on + strand, within GFF1454at 1481.305 kb on - strand, within GFF1454at 1481.402 kb on + strand, within GFF1454at 1481.415 kb on + strand, within GFF1454at 1481.436 kb on - strand, within GFF1454at 1481.438 kb on + strand, within GFF1454at 1481.446 kb on - strand, within GFF1454at 1481.462 kb on + strand, within GFF1454at 1481.467 kb on + strand, within GFF1454at 1481.470 kb on - strand, within GFF1454at 1481.475 kb on - strand, within GFF1454at 1481.513 kb on + strand, within GFF1454at 1481.626 kb on - strand, within GFF1454at 1481.626 kb on - strand, within GFF1454at 1481.653 kb on - strand, within GFF1454at 1481.653 kb on - strand, within GFF1454

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Per-strain Table

Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine
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1,478,656 + -1.4
1,478,656 + +0.4
1,478,657 + -0.2
1,478,664 - -0.4
1,478,685 - -0.4
1,478,950 + GFF1451 0.75 -0.2
1,478,950 + GFF1451 0.75 +1.0
1,478,950 + GFF1451 0.75 -1.3
1,478,958 - GFF1451 0.78 -1.4
1,478,968 + GFF1451 0.81 +0.9
1,478,973 + GFF1451 0.83 -0.1
1,478,976 - GFF1451 0.84 -1.8
1,479,003 + +1.3
1,479,025 - -1.2
1,479,041 + -1.8
1,479,049 - -1.4
1,479,113 + -1.2
1,479,176 + -2.2
1,479,176 + -1.2
1,479,184 - -1.6
1,479,378 - GFF1452 0.22 -1.4
1,479,412 - GFF1452 0.24 -1.7
1,480,104 - GFF1452 0.66 -1.2
1,480,647 + -0.6
1,480,726 + GFF1453 0.17 +0.1
1,480,734 - GFF1453 0.19 -1.5
1,481,026 + GFF1453 0.85 +0.2
1,481,030 - GFF1453 0.86 +1.0
1,481,053 - +1.2
1,481,055 + -1.3
1,481,121 + -1.4
1,481,142 + -1.9
1,481,190 + -1.4
1,481,215 - GFF1454 0.12 -1.6
1,481,215 - GFF1454 0.12 -2.8
1,481,215 - GFF1454 0.12 -0.5
1,481,227 - GFF1454 0.14 -1.2
1,481,273 + GFF1454 0.20 -2.1
1,481,305 - GFF1454 0.24 -1.8
1,481,402 + GFF1454 0.37 -2.3
1,481,415 + GFF1454 0.38 -1.2
1,481,436 - GFF1454 0.41 -2.4
1,481,438 + GFF1454 0.41 -0.5
1,481,446 - GFF1454 0.42 -1.4
1,481,462 + GFF1454 0.45 -1.4
1,481,467 + GFF1454 0.45 -1.5
1,481,470 - GFF1454 0.46 -1.9
1,481,475 - GFF1454 0.46 -1.8
1,481,513 + GFF1454 0.51 -1.2
1,481,626 - GFF1454 0.66 -1.8
1,481,626 - GFF1454 0.66 -1.2
1,481,653 - GFF1454 0.70 -1.4
1,481,653 - GFF1454 0.70 -1.4

Or see this region's nucleotide sequence