Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5250 and GFF5251 are separated by 561 nucleotides GFF5251 and GFF5252 overlap by 22 nucleotides GFF5252 and GFF5253 are separated by 65 nucleotides GFF5253 and GFF5254 overlap by 8 nucleotides GFF5254 and GFF5255 are separated by 168 nucleotides
GFF5250 - Replication protein, at 5,244,748 to 5,245,407
GFF5250
GFF5251 - Death on curing protein, Doc toxin, at 5,245,969 to 5,246,274
GFF5251
GFF5252 - Prevent host death protein, Phd antitoxin, at 5,246,253 to 5,246,525
GFF5252
GFF5253 - hypothetical protein, at 5,246,591 to 5,247,034
GFF5253
GFF5254 - Mobilization protein, at 5,247,027 to 5,247,302
GFF5254
GFF5255 - Mobilization protein MobA @ Conjugal transfer protein TraA, at 5,247,471 to 5,248,682
GFF5255
Position (kb) 5246
5247 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5245.300 kb on - strand, within GFF5250 at 5245.411 kb on - strand at 5245.967 kb on + strand at 5246.001 kb on + strand, within GFF5251 at 5246.001 kb on + strand, within GFF5251 at 5246.002 kb on + strand, within GFF5251 at 5246.002 kb on + strand, within GFF5251 at 5246.002 kb on + strand, within GFF5251 at 5246.072 kb on + strand, within GFF5251 at 5246.094 kb on + strand, within GFF5251 at 5246.141 kb on + strand, within GFF5251 at 5246.236 kb on + strand, within GFF5251 at 5246.282 kb on + strand, within GFF5252 at 5246.409 kb on + strand, within GFF5252 at 5246.419 kb on + strand, within GFF5252 at 5246.483 kb on - strand, within GFF5252 at 5246.589 kb on + strand at 5246.607 kb on - strand at 5246.628 kb on + strand at 5246.642 kb on - strand, within GFF5253 at 5246.674 kb on + strand, within GFF5253 at 5246.695 kb on + strand, within GFF5253 at 5246.711 kb on + strand, within GFF5253 at 5246.729 kb on + strand, within GFF5253 at 5246.729 kb on + strand, within GFF5253 at 5246.729 kb on + strand, within GFF5253 at 5246.729 kb on + strand, within GFF5253 at 5246.729 kb on + strand, within GFF5253 at 5246.737 kb on - strand, within GFF5253 at 5246.740 kb on + strand, within GFF5253 at 5246.747 kb on - strand, within GFF5253 at 5246.820 kb on + strand, within GFF5253 at 5246.960 kb on - strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.962 kb on + strand, within GFF5253 at 5246.970 kb on - strand, within GFF5253 at 5246.970 kb on - strand, within GFF5253 at 5246.970 kb on - strand, within GFF5253 at 5246.970 kb on - strand, within GFF5253 at 5246.970 kb on - strand, within GFF5253 at 5246.983 kb on + strand, within GFF5253 at 5247.017 kb on + strand at 5247.038 kb on - strand at 5247.055 kb on + strand, within GFF5254 at 5247.055 kb on + strand, within GFF5254 at 5247.063 kb on + strand, within GFF5254 at 5247.070 kb on + strand, within GFF5254 at 5247.081 kb on - strand, within GFF5254 at 5247.135 kb on + strand, within GFF5254 at 5247.170 kb on + strand, within GFF5254 at 5247.193 kb on + strand, within GFF5254 at 5247.201 kb on + strand, within GFF5254 at 5247.201 kb on + strand, within GFF5254 at 5247.201 kb on + strand, within GFF5254 at 5247.214 kb on - strand, within GFF5254 at 5247.250 kb on + strand, within GFF5254 at 5247.257 kb on - strand, within GFF5254 at 5247.257 kb on - strand, within GFF5254
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 5,245,300 - GFF5250 0.84 +0.7 5,245,411 - +2.1 5,245,967 + -3.2 5,246,001 + GFF5251 0.10 +0.5 5,246,001 + GFF5251 0.10 -0.1 5,246,002 + GFF5251 0.11 -1.4 5,246,002 + GFF5251 0.11 -0.2 5,246,002 + GFF5251 0.11 -0.6 5,246,072 + GFF5251 0.34 -2.9 5,246,094 + GFF5251 0.41 -0.7 5,246,141 + GFF5251 0.56 +0.1 5,246,236 + GFF5251 0.87 -0.9 5,246,282 + GFF5252 0.11 -1.6 5,246,409 + GFF5252 0.57 -1.1 5,246,419 + GFF5252 0.61 -1.1 5,246,483 - GFF5252 0.84 -2.1 5,246,589 + -1.6 5,246,607 - -2.2 5,246,628 + -1.4 5,246,642 - GFF5253 0.11 +0.3 5,246,674 + GFF5253 0.19 -0.6 5,246,695 + GFF5253 0.23 -0.8 5,246,711 + GFF5253 0.27 -0.9 5,246,729 + GFF5253 0.31 -0.4 5,246,729 + GFF5253 0.31 -1.8 5,246,729 + GFF5253 0.31 -1.5 5,246,729 + GFF5253 0.31 -1.5 5,246,729 + GFF5253 0.31 -2.2 5,246,737 - GFF5253 0.33 +0.9 5,246,740 + GFF5253 0.34 -3.3 5,246,747 - GFF5253 0.35 -0.9 5,246,820 + GFF5253 0.52 -0.5 5,246,960 - GFF5253 0.83 -1.5 5,246,962 + GFF5253 0.84 -2.1 5,246,962 + GFF5253 0.84 -1.8 5,246,962 + GFF5253 0.84 -1.9 5,246,962 + GFF5253 0.84 -0.0 5,246,962 + GFF5253 0.84 -0.9 5,246,962 + GFF5253 0.84 -2.4 5,246,962 + GFF5253 0.84 -1.1 5,246,962 + GFF5253 0.84 -1.8 5,246,962 + GFF5253 0.84 -0.1 5,246,962 + GFF5253 0.84 -3.3 5,246,970 - GFF5253 0.85 +0.9 5,246,970 - GFF5253 0.85 -1.1 5,246,970 - GFF5253 0.85 -2.8 5,246,970 - GFF5253 0.85 +0.3 5,246,970 - GFF5253 0.85 -1.4 5,246,983 + GFF5253 0.88 +1.2 5,247,017 + -0.7 5,247,038 - -2.8 5,247,055 + GFF5254 0.10 -2.0 5,247,055 + GFF5254 0.10 -2.5 5,247,063 + GFF5254 0.13 -1.5 5,247,070 + GFF5254 0.16 -1.1 5,247,081 - GFF5254 0.20 -1.7 5,247,135 + GFF5254 0.39 -0.0 5,247,170 + GFF5254 0.52 -0.8 5,247,193 + GFF5254 0.60 -1.7 5,247,201 + GFF5254 0.63 +1.9 5,247,201 + GFF5254 0.63 -1.4 5,247,201 + GFF5254 0.63 -1.2 5,247,214 - GFF5254 0.68 -0.7 5,247,250 + GFF5254 0.81 -1.3 5,247,257 - GFF5254 0.83 -1.8 5,247,257 - GFF5254 0.83 -1.5
Or see this region's nucleotide sequence