Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF30 and GFF31 are separated by 299 nucleotides GFF31 and GFF32 are separated by 77 nucleotides GFF32 and GFF33 are separated by 98 nucleotides GFF33 and GFF34 are separated by 62 nucleotides GFF34 and GFF35 are separated by 160 nucleotides GFF35 and GFF36 are separated by 79 nucleotides
GFF30 - hypothetical protein, at 26,288 to 26,803
GFF30
GFF31 - Phage lysin (EC 3.2.1.17) # Phage lysozyme or muramidase (EC 3.2.1.17), at 27,103 to 27,312
GFF31
GFF32 - hypothetical protein, at 27,390 to 27,506
GFF32
GFF33 - hypothetical protein, at 27,605 to 27,721
GFF33
GFF34 - hypothetical protein, at 27,784 to 27,912
GFF34
GFF35 - hypothetical protein, at 28,073 to 28,234
GFF35
GFF36 - hypothetical protein, at 28,314 to 28,754
GFF36
Position (kb)
27
28 Strain fitness (log2 ratio)
-2
-1
0
1 at 26.642 kb on + strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.650 kb on - strand, within GFF30 at 26.655 kb on + strand, within GFF30 at 26.685 kb on - strand, within GFF30 at 26.952 kb on + strand at 26.981 kb on + strand at 26.987 kb on + strand at 26.994 kb on - strand at 27.004 kb on - strand at 27.026 kb on + strand at 27.026 kb on - strand at 27.111 kb on + strand at 27.127 kb on + strand, within GFF31 at 27.135 kb on - strand, within GFF31 at 27.137 kb on - strand, within GFF31 at 27.197 kb on + strand, within GFF31 at 27.200 kb on - strand, within GFF31 at 27.205 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.256 kb on + strand, within GFF31 at 27.264 kb on - strand, within GFF31 at 27.278 kb on - strand, within GFF31 at 27.309 kb on - strand at 27.340 kb on - strand at 27.369 kb on - strand at 27.388 kb on + strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.396 kb on - strand at 27.407 kb on + strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.410 kb on - strand, within GFF32 at 27.441 kb on + strand, within GFF32 at 27.556 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.591 kb on + strand at 27.599 kb on - strand at 27.599 kb on - strand at 27.606 kb on + strand at 27.650 kb on - strand, within GFF33 at 27.700 kb on - strand, within GFF33 at 27.713 kb on - strand at 27.729 kb on + strand at 27.731 kb on - strand at 27.731 kb on - strand at 27.823 kb on - strand, within GFF34 at 27.924 kb on - strand at 27.928 kb on + strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.940 kb on + strand at 27.950 kb on + strand at 27.958 kb on - strand at 27.980 kb on - strand at 27.981 kb on + strand at 27.981 kb on - strand at 27.985 kb on - strand at 27.994 kb on + strand at 28.000 kb on + strand at 28.005 kb on + strand at 28.005 kb on + strand at 28.008 kb on - strand at 28.014 kb on + strand at 28.028 kb on - strand at 28.039 kb on + strand at 28.055 kb on - strand at 28.056 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.063 kb on - strand at 28.086 kb on + strand at 28.087 kb on + strand at 28.094 kb on - strand, within GFF35 at 28.144 kb on - strand, within GFF35 at 28.164 kb on + strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.172 kb on - strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.215 kb on + strand, within GFF35 at 28.223 kb on - strand at 28.311 kb on + strand at 28.311 kb on + strand at 28.369 kb on + strand, within GFF36 at 28.373 kb on + strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.387 kb on - strand, within GFF36 at 28.425 kb on - strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.433 kb on + strand, within GFF36 at 28.436 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.441 kb on - strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.443 kb on + strand, within GFF36 at 28.445 kb on + strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.451 kb on - strand, within GFF36 at 28.457 kb on + strand, within GFF36 at 28.467 kb on + strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.475 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.498 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.514 kb on - strand, within GFF36 at 28.521 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.616 kb on + strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.624 kb on - strand, within GFF36 at 28.627 kb on - strand, within GFF36 at 28.658 kb on + strand, within GFF36 at 28.659 kb on + strand, within GFF36 at 28.660 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.666 kb on - strand, within GFF36 at 28.667 kb on - strand, within GFF36
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 26,642 + GFF30 0.69 -1.2 26,650 - GFF30 0.70 -0.3 26,650 - GFF30 0.70 +0.8 26,650 - GFF30 0.70 +0.8 26,655 + GFF30 0.71 -0.2 26,685 - GFF30 0.77 -0.6 26,952 + -1.1 26,981 + -1.2 26,987 + +1.1 26,994 - +0.4 27,004 - +0.2 27,026 + +0.9 27,026 - -0.1 27,111 + -0.3 27,127 + GFF31 0.11 -0.7 27,135 - GFF31 0.15 -0.0 27,137 - GFF31 0.16 -0.4 27,197 + GFF31 0.45 +0.3 27,200 - GFF31 0.46 +0.7 27,205 + GFF31 0.49 -0.0 27,256 + GFF31 0.73 +0.1 27,256 + GFF31 0.73 +0.1 27,264 - GFF31 0.77 +0.1 27,278 - GFF31 0.83 +0.1 27,309 - +0.2 27,340 - +1.5 27,369 - +0.6 27,388 + -0.7 27,396 - -0.4 27,396 - -1.6 27,396 - -0.1 27,407 + GFF32 0.15 +0.4 27,410 - GFF32 0.17 +0.2 27,410 - GFF32 0.17 -0.9 27,410 - GFF32 0.17 -1.2 27,410 - GFF32 0.17 +1.3 27,441 + GFF32 0.44 +0.6 27,556 + +0.7 27,591 + -1.6 27,591 + -2.2 27,591 + +0.3 27,591 + +0.1 27,591 + +1.3 27,591 + -1.5 27,599 - -0.2 27,599 - +1.3 27,606 + -1.6 27,650 - GFF33 0.38 -0.6 27,700 - GFF33 0.81 -1.8 27,713 - +1.1 27,729 + -1.1 27,731 - -0.4 27,731 - +0.4 27,823 - GFF34 0.30 -1.0 27,924 - -0.2 27,928 + +0.0 27,940 + +0.8 27,940 + -1.5 27,940 + +1.2 27,940 + -0.4 27,950 + +0.1 27,958 - -0.0 27,980 - -0.1 27,981 + -0.7 27,981 - +0.1 27,985 - -0.8 27,994 + -0.4 28,000 + -0.2 28,005 + -0.5 28,005 + +0.3 28,008 - +0.8 28,014 + +0.8 28,028 - -0.7 28,039 + +0.4 28,055 - -0.1 28,056 - +0.6 28,063 - +0.9 28,063 - +0.1 28,063 - -0.5 28,063 - -0.4 28,086 + +0.0 28,087 + +1.0 28,094 - GFF35 0.13 +1.2 28,144 - GFF35 0.44 +0.4 28,164 + GFF35 0.56 -0.6 28,172 - GFF35 0.61 +0.5 28,172 - GFF35 0.61 -1.6 28,172 - GFF35 0.61 +0.1 28,172 - GFF35 0.61 +0.4 28,172 - GFF35 0.61 +0.6 28,215 + GFF35 0.88 +0.2 28,215 + GFF35 0.88 -0.8 28,223 - -1.4 28,311 + +0.2 28,311 + -0.6 28,369 + GFF36 0.12 -0.3 28,373 + GFF36 0.13 -0.4 28,387 - GFF36 0.17 -0.0 28,387 - GFF36 0.17 +0.9 28,425 - GFF36 0.25 +1.0 28,433 + GFF36 0.27 -0.4 28,433 + GFF36 0.27 +1.1 28,436 - GFF36 0.28 -1.8 28,441 - GFF36 0.29 -1.2 28,441 - GFF36 0.29 +0.4 28,441 - GFF36 0.29 +0.9 28,441 - GFF36 0.29 +0.3 28,441 - GFF36 0.29 +1.0 28,443 + GFF36 0.29 +0.4 28,443 + GFF36 0.29 -0.3 28,443 + GFF36 0.29 -0.3 28,445 + GFF36 0.30 +0.8 28,451 - GFF36 0.31 -0.3 28,451 - GFF36 0.31 +0.5 28,451 - GFF36 0.31 -2.7 28,457 + GFF36 0.32 -0.7 28,467 + GFF36 0.35 +0.6 28,475 - GFF36 0.37 +0.1 28,475 - GFF36 0.37 -0.7 28,498 - GFF36 0.42 +0.6 28,498 - GFF36 0.42 +0.3 28,498 - GFF36 0.42 -0.2 28,498 - GFF36 0.42 +0.0 28,514 - GFF36 0.45 +0.2 28,514 - GFF36 0.45 -0.5 28,521 + GFF36 0.47 +0.5 28,616 + GFF36 0.68 -1.2 28,616 + GFF36 0.68 +0.5 28,616 + GFF36 0.68 -0.6 28,624 - GFF36 0.70 +0.4 28,624 - GFF36 0.70 +0.6 28,624 - GFF36 0.70 +0.3 28,624 - GFF36 0.70 -0.2 28,624 - GFF36 0.70 -0.3 28,627 - GFF36 0.71 +0.4 28,658 + GFF36 0.78 -0.7 28,659 + GFF36 0.78 +0.1 28,660 - GFF36 0.78 +0.3 28,666 - GFF36 0.80 +0.2 28,666 - GFF36 0.80 +0.7 28,666 - GFF36 0.80 +0.1 28,666 - GFF36 0.80 -2.6 28,666 - GFF36 0.80 +0.3 28,667 - GFF36 0.80 -0.2
Or see this region's nucleotide sequence