Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3057 and GFF3058 are separated by 98 nucleotides GFF3058 and GFF3059 are separated by 28 nucleotides GFF3059 and GFF3060 overlap by 4 nucleotides GFF3060 and GFF3061 are separated by 88 nucleotides GFF3061 and GFF3062 are separated by 120 nucleotides
GFF3057 - ClpXP protease specificity-enhancing factor SspBalpha, at 3,132,286 to 3,132,762
GFF3057
GFF3058 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 3,132,861 to 3,133,448
GFF3058
GFF3059 - hypothetical protein, at 3,133,477 to 3,133,764
GFF3059
GFF3060 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 3,133,761 to 3,134,375
GFF3060
GFF3061 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 3,134,464 to 3,135,201
GFF3061
GFF3062 - Imidazole glycerol phosphate synthase cyclase subunit, at 3,135,322 to 3,136,086
GFF3062
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3132.774 kb on - strand at 3132.795 kb on + strand at 3132.799 kb on + strand at 3132.803 kb on - strand at 3132.843 kb on + strand at 3132.845 kb on + strand at 3132.847 kb on + strand at 3132.853 kb on - strand at 3132.861 kb on - strand at 3132.870 kb on + strand at 3132.881 kb on + strand at 3132.881 kb on + strand at 3132.934 kb on + strand, within GFF3058 at 3132.941 kb on - strand, within GFF3058 at 3132.941 kb on - strand, within GFF3058 at 3132.971 kb on + strand, within GFF3058 at 3132.972 kb on - strand, within GFF3058 at 3133.016 kb on + strand, within GFF3058 at 3133.024 kb on - strand, within GFF3058 at 3133.039 kb on + strand, within GFF3058 at 3133.049 kb on + strand, within GFF3058 at 3133.057 kb on - strand, within GFF3058 at 3133.057 kb on - strand, within GFF3058 at 3133.058 kb on - strand, within GFF3058 at 3133.059 kb on + strand, within GFF3058 at 3133.063 kb on + strand, within GFF3058 at 3133.064 kb on + strand, within GFF3058 at 3133.089 kb on + strand, within GFF3058 at 3133.092 kb on - strand, within GFF3058 at 3133.094 kb on + strand, within GFF3058 at 3133.094 kb on + strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.128 kb on - strand, within GFF3058 at 3133.169 kb on - strand, within GFF3058 at 3133.172 kb on + strand, within GFF3058 at 3133.195 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.201 kb on + strand, within GFF3058 at 3133.204 kb on - strand, within GFF3058 at 3133.217 kb on + strand, within GFF3058 at 3133.217 kb on + strand, within GFF3058 at 3133.223 kb on - strand, within GFF3058 at 3133.227 kb on - strand, within GFF3058 at 3133.279 kb on + strand, within GFF3058 at 3133.279 kb on + strand, within GFF3058 at 3133.283 kb on + strand, within GFF3058 at 3133.284 kb on + strand, within GFF3058 at 3133.287 kb on - strand, within GFF3058 at 3133.291 kb on - strand, within GFF3058 at 3133.317 kb on - strand, within GFF3058 at 3133.319 kb on + strand, within GFF3058 at 3133.319 kb on + strand, within GFF3058 at 3133.322 kb on - strand, within GFF3058 at 3133.365 kb on + strand, within GFF3058 at 3133.365 kb on + strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.382 kb on + strand, within GFF3058 at 3133.390 kb on - strand at 3133.390 kb on - strand at 3133.392 kb on + strand at 3133.397 kb on - strand at 3133.400 kb on - strand at 3133.400 kb on - strand at 3133.413 kb on + strand at 3133.414 kb on - strand at 3133.430 kb on + strand at 3133.437 kb on + strand at 3133.439 kb on - strand at 3133.450 kb on - strand at 3133.450 kb on - strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.499 kb on + strand at 3133.502 kb on - strand at 3133.502 kb on - strand at 3133.502 kb on - strand at 3133.548 kb on - strand, within GFF3059 at 3133.550 kb on + strand, within GFF3059 at 3133.550 kb on + strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.575 kb on - strand, within GFF3059 at 3133.647 kb on - strand, within GFF3059 at 3133.667 kb on - strand, within GFF3059 at 3133.667 kb on - strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.715 kb on - strand, within GFF3059 at 3133.715 kb on - strand, within GFF3059 at 3133.741 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.761 kb on - strand at 3133.763 kb on + strand at 3133.765 kb on + strand at 3133.765 kb on + strand at 3133.765 kb on + strand at 3133.773 kb on - strand at 3133.775 kb on + strand at 3133.803 kb on - strand at 3133.806 kb on + strand at 3133.810 kb on + strand at 3133.814 kb on - strand at 3133.814 kb on - strand at 3133.815 kb on + strand at 3133.815 kb on + strand at 3133.815 kb on + strand at 3133.815 kb on + strand at 3133.815 kb on + strand at 3133.816 kb on - strand at 3133.818 kb on - strand at 3133.818 kb on - strand at 3133.818 kb on - strand at 3133.818 kb on - strand at 3133.818 kb on - strand at 3133.820 kb on + strand at 3133.820 kb on + strand at 3133.820 kb on + strand at 3133.823 kb on - strand, within GFF3060 at 3133.823 kb on - strand, within GFF3060 at 3133.823 kb on - strand, within GFF3060 at 3133.828 kb on - strand, within GFF3060 at 3133.828 kb on - strand, within GFF3060 at 3133.898 kb on - strand, within GFF3060 at 3133.972 kb on - strand, within GFF3060 at 3133.989 kb on - strand, within GFF3060 at 3134.029 kb on - strand, within GFF3060 at 3134.054 kb on + strand, within GFF3060 at 3134.061 kb on + strand, within GFF3060 at 3134.061 kb on + strand, within GFF3060 at 3134.061 kb on + strand, within GFF3060 at 3134.066 kb on + strand, within GFF3060 at 3134.083 kb on + strand, within GFF3060 at 3134.087 kb on + strand, within GFF3060 at 3134.117 kb on - strand, within GFF3060 at 3134.156 kb on - strand, within GFF3060 at 3134.194 kb on - strand, within GFF3060 at 3134.201 kb on - strand, within GFF3060 at 3134.229 kb on + strand, within GFF3060 at 3134.300 kb on - strand, within GFF3060 at 3134.345 kb on - strand at 3134.407 kb on + strand at 3134.407 kb on + strand at 3134.415 kb on - strand at 3134.436 kb on - strand at 3134.436 kb on - strand at 3134.510 kb on + strand at 3134.516 kb on + strand at 3134.528 kb on - strand at 3134.530 kb on + strand at 3134.531 kb on + strand at 3134.580 kb on + strand, within GFF3061 at 3134.654 kb on + strand, within GFF3061 at 3134.654 kb on + strand, within GFF3061 at 3134.673 kb on - strand, within GFF3061 at 3134.685 kb on - strand, within GFF3061 at 3134.744 kb on + strand, within GFF3061 at 3134.744 kb on + strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.752 kb on - strand, within GFF3061 at 3134.770 kb on + strand, within GFF3061 at 3134.778 kb on - strand, within GFF3061 at 3134.805 kb on + strand, within GFF3061 at 3134.826 kb on - strand, within GFF3061 at 3134.986 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.988 kb on + strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3134.996 kb on - strand, within GFF3061 at 3135.032 kb on + strand, within GFF3061 at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.242 kb on - strand at 3135.244 kb on + strand at 3135.247 kb on - strand at 3135.287 kb on - strand at 3135.339 kb on + strand at 3135.350 kb on + strand at 3135.358 kb on - strand at 3135.368 kb on + strand at 3135.375 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,132,774 - -0.3 3,132,795 + +1.3 3,132,799 + -0.3 3,132,803 - -0.1 3,132,843 + -0.2 3,132,845 + -2.0 3,132,847 + +0.9 3,132,853 - -1.5 3,132,861 - -1.5 3,132,870 + -3.1 3,132,881 + -2.0 3,132,881 + -1.0 3,132,934 + GFF3058 0.12 -1.0 3,132,941 - GFF3058 0.14 -1.8 3,132,941 - GFF3058 0.14 -2.4 3,132,971 + GFF3058 0.19 -1.7 3,132,972 - GFF3058 0.19 -1.5 3,133,016 + GFF3058 0.26 -2.8 3,133,024 - GFF3058 0.28 -1.7 3,133,039 + GFF3058 0.30 -2.9 3,133,049 + GFF3058 0.32 -2.8 3,133,057 - GFF3058 0.33 -1.5 3,133,057 - GFF3058 0.33 -1.8 3,133,058 - GFF3058 0.34 -1.7 3,133,059 + GFF3058 0.34 -1.2 3,133,063 + GFF3058 0.34 -2.2 3,133,064 + GFF3058 0.35 -2.6 3,133,089 + GFF3058 0.39 -2.0 3,133,092 - GFF3058 0.39 -0.6 3,133,094 + GFF3058 0.40 -1.5 3,133,094 + GFF3058 0.40 -1.6 3,133,102 - GFF3058 0.41 -3.1 3,133,102 - GFF3058 0.41 -1.9 3,133,102 - GFF3058 0.41 -3.4 3,133,102 - GFF3058 0.41 -1.9 3,133,128 - GFF3058 0.45 -2.5 3,133,169 - GFF3058 0.52 -3.3 3,133,172 + GFF3058 0.53 -0.7 3,133,195 + GFF3058 0.57 -1.6 3,133,196 + GFF3058 0.57 -2.7 3,133,196 + GFF3058 0.57 -2.0 3,133,196 + GFF3058 0.57 -2.9 3,133,201 + GFF3058 0.58 -1.6 3,133,204 - GFF3058 0.58 -1.2 3,133,217 + GFF3058 0.61 +0.1 3,133,217 + GFF3058 0.61 -3.5 3,133,223 - GFF3058 0.62 -1.3 3,133,227 - GFF3058 0.62 -1.0 3,133,279 + GFF3058 0.71 -1.9 3,133,279 + GFF3058 0.71 -1.8 3,133,283 + GFF3058 0.72 -3.1 3,133,284 + GFF3058 0.72 -2.8 3,133,287 - GFF3058 0.72 -0.6 3,133,291 - GFF3058 0.73 -2.0 3,133,317 - GFF3058 0.78 -2.8 3,133,319 + GFF3058 0.78 -2.2 3,133,319 + GFF3058 0.78 -1.2 3,133,322 - GFF3058 0.78 -1.2 3,133,365 + GFF3058 0.86 -2.1 3,133,365 + GFF3058 0.86 -2.0 3,133,373 - GFF3058 0.87 -1.1 3,133,373 - GFF3058 0.87 -2.2 3,133,373 - GFF3058 0.87 -0.0 3,133,373 - GFF3058 0.87 -2.3 3,133,373 - GFF3058 0.87 -0.2 3,133,382 + GFF3058 0.89 -1.9 3,133,390 - -3.2 3,133,390 - -1.3 3,133,392 + -2.7 3,133,397 - +1.0 3,133,400 - -1.8 3,133,400 - -2.8 3,133,413 + -1.3 3,133,414 - -0.0 3,133,430 + +0.1 3,133,437 + -1.1 3,133,439 - -2.5 3,133,450 - -0.2 3,133,450 - +0.7 3,133,494 + -1.1 3,133,494 + +0.9 3,133,494 + +0.2 3,133,494 + +0.9 3,133,494 + +0.1 3,133,499 + -0.9 3,133,502 - -0.7 3,133,502 - -1.2 3,133,502 - -0.9 3,133,548 - GFF3059 0.25 -1.6 3,133,550 + GFF3059 0.25 -0.9 3,133,550 + GFF3059 0.25 -2.9 3,133,558 - GFF3059 0.28 -2.8 3,133,558 - GFF3059 0.28 -2.0 3,133,558 - GFF3059 0.28 -2.6 3,133,558 - GFF3059 0.28 -2.4 3,133,575 - GFF3059 0.34 -1.3 3,133,647 - GFF3059 0.59 -0.8 3,133,667 - GFF3059 0.66 -1.6 3,133,667 - GFF3059 0.66 -2.5 3,133,697 + GFF3059 0.76 -0.5 3,133,697 + GFF3059 0.76 -0.0 3,133,697 + GFF3059 0.76 -0.2 3,133,697 + GFF3059 0.76 -0.2 3,133,697 + GFF3059 0.76 -0.9 3,133,697 + GFF3059 0.76 +1.6 3,133,705 - GFF3059 0.79 -0.5 3,133,705 - GFF3059 0.79 -2.7 3,133,705 - GFF3059 0.79 -0.8 3,133,715 - GFF3059 0.83 -1.7 3,133,715 - GFF3059 0.83 -2.5 3,133,741 + +0.3 3,133,758 + +0.3 3,133,758 + -0.2 3,133,758 + -1.6 3,133,758 + -0.7 3,133,761 - -3.0 3,133,763 + +0.4 3,133,765 + -1.6 3,133,765 + -0.5 3,133,765 + -2.4 3,133,773 - -2.5 3,133,775 + -3.5 3,133,803 - -3.0 3,133,806 + -3.4 3,133,810 + -2.0 3,133,814 - -2.9 3,133,814 - -2.8 3,133,815 + +0.2 3,133,815 + -2.8 3,133,815 + -2.2 3,133,815 + -2.5 3,133,815 + -3.7 3,133,816 - -2.1 3,133,818 - -3.2 3,133,818 - -3.2 3,133,818 - -2.8 3,133,818 - -2.5 3,133,818 - -0.3 3,133,820 + -1.8 3,133,820 + -1.7 3,133,820 + +0.6 3,133,823 - GFF3060 0.10 -2.0 3,133,823 - GFF3060 0.10 -2.1 3,133,823 - GFF3060 0.10 -2.9 3,133,828 - GFF3060 0.11 -0.6 3,133,828 - GFF3060 0.11 -2.1 3,133,898 - GFF3060 0.22 -3.4 3,133,972 - GFF3060 0.34 -1.7 3,133,989 - GFF3060 0.37 -1.3 3,134,029 - GFF3060 0.44 -0.0 3,134,054 + GFF3060 0.48 -1.8 3,134,061 + GFF3060 0.49 -1.1 3,134,061 + GFF3060 0.49 -0.7 3,134,061 + GFF3060 0.49 -2.5 3,134,066 + GFF3060 0.50 -3.4 3,134,083 + GFF3060 0.52 -0.3 3,134,087 + GFF3060 0.53 -2.8 3,134,117 - GFF3060 0.58 -2.0 3,134,156 - GFF3060 0.64 -2.0 3,134,194 - GFF3060 0.70 -2.4 3,134,201 - GFF3060 0.72 -1.6 3,134,229 + GFF3060 0.76 -1.9 3,134,300 - GFF3060 0.88 -3.5 3,134,345 - -2.7 3,134,407 + +1.7 3,134,407 + +0.2 3,134,415 - -0.6 3,134,436 - -0.8 3,134,436 - +0.0 3,134,510 + -2.7 3,134,516 + -2.2 3,134,528 - -2.1 3,134,530 + -1.7 3,134,531 + +0.4 3,134,580 + GFF3061 0.16 -1.5 3,134,654 + GFF3061 0.26 -1.2 3,134,654 + GFF3061 0.26 -1.5 3,134,673 - GFF3061 0.28 -1.2 3,134,685 - GFF3061 0.30 -1.8 3,134,744 + GFF3061 0.38 -2.8 3,134,744 + GFF3061 0.38 -1.8 3,134,752 - GFF3061 0.39 -1.0 3,134,752 - GFF3061 0.39 -4.1 3,134,752 - GFF3061 0.39 -1.5 3,134,752 - GFF3061 0.39 -2.3 3,134,752 - GFF3061 0.39 -2.2 3,134,752 - GFF3061 0.39 -2.0 3,134,752 - GFF3061 0.39 -2.4 3,134,770 + GFF3061 0.41 -2.6 3,134,778 - GFF3061 0.43 -1.1 3,134,805 + GFF3061 0.46 -1.9 3,134,826 - GFF3061 0.49 -1.0 3,134,986 + GFF3061 0.71 -0.3 3,134,988 + GFF3061 0.71 -3.1 3,134,988 + GFF3061 0.71 -2.2 3,134,988 + GFF3061 0.71 -1.0 3,134,988 + GFF3061 0.71 -2.0 3,134,988 + GFF3061 0.71 -1.5 3,134,988 + GFF3061 0.71 -2.0 3,134,988 + GFF3061 0.71 -1.6 3,134,988 + GFF3061 0.71 -0.2 3,134,988 + GFF3061 0.71 -1.3 3,134,988 + GFF3061 0.71 -1.7 3,134,996 - GFF3061 0.72 -2.1 3,134,996 - GFF3061 0.72 -2.5 3,134,996 - GFF3061 0.72 -3.5 3,134,996 - GFF3061 0.72 -2.4 3,134,996 - GFF3061 0.72 -2.8 3,134,996 - GFF3061 0.72 -2.4 3,134,996 - GFF3061 0.72 -0.9 3,134,996 - GFF3061 0.72 -2.8 3,135,032 + GFF3061 0.77 -2.2 3,135,242 - -2.1 3,135,242 - -2.2 3,135,242 - +0.5 3,135,242 - +1.4 3,135,242 - -0.4 3,135,242 - +0.4 3,135,242 - +0.5 3,135,242 - -2.0 3,135,242 - +0.4 3,135,242 - -0.6 3,135,244 + -1.3 3,135,247 - -0.2 3,135,287 - -0.8 3,135,339 + -3.0 3,135,350 + -2.6 3,135,358 - -4.4 3,135,368 + -2.3 3,135,375 + -2.7
Or see this region's nucleotide sequence