Experiment: EMM_noCarbon with Sucrose and Putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3055 and GFF3056 are separated by 225 nucleotides GFF3056 and GFF3057 are separated by 27 nucleotides GFF3057 and GFF3058 are separated by 98 nucleotides GFF3058 and GFF3059 are separated by 28 nucleotides
GFF3055 - Fumarate hydratase class II (EC 4.2.1.2), at 3,129,958 to 3,131,343
GFF3055
GFF3056 - hypothetical protein, at 3,131,569 to 3,132,258
GFF3056
GFF3057 - ClpXP protease specificity-enhancing factor SspBalpha, at 3,132,286 to 3,132,762
GFF3057
GFF3058 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 3,132,861 to 3,133,448
GFF3058
GFF3059 - hypothetical protein, at 3,133,477 to 3,133,764
GFF3059
GFF3060 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 3,133,761 to 3,134,375
GFF3060
Position (kb)
3132
3133 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3131.334 kb on - strand at 3131.393 kb on - strand at 3131.469 kb on + strand at 3131.481 kb on + strand at 3131.530 kb on + strand at 3131.557 kb on - strand at 3131.587 kb on - strand at 3131.593 kb on - strand at 3131.593 kb on - strand at 3131.593 kb on - strand at 3131.598 kb on + strand at 3131.623 kb on - strand at 3131.628 kb on - strand at 3131.628 kb on - strand at 3131.682 kb on + strand, within GFF3056 at 3131.718 kb on + strand, within GFF3056 at 3131.718 kb on + strand, within GFF3056 at 3131.718 kb on + strand, within GFF3056 at 3131.801 kb on + strand, within GFF3056 at 3131.803 kb on + strand, within GFF3056 at 3131.805 kb on + strand, within GFF3056 at 3131.818 kb on + strand, within GFF3056 at 3131.818 kb on + strand, within GFF3056 at 3131.826 kb on - strand, within GFF3056 at 3131.901 kb on + strand, within GFF3056 at 3131.901 kb on + strand, within GFF3056 at 3131.911 kb on + strand, within GFF3056 at 3131.944 kb on + strand, within GFF3056 at 3131.951 kb on + strand, within GFF3056 at 3131.956 kb on + strand, within GFF3056 at 3131.956 kb on + strand, within GFF3056 at 3131.977 kb on - strand, within GFF3056 at 3132.005 kb on - strand, within GFF3056 at 3132.007 kb on - strand, within GFF3056 at 3132.046 kb on - strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.068 kb on + strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.076 kb on - strand, within GFF3056 at 3132.133 kb on + strand, within GFF3056 at 3132.140 kb on + strand, within GFF3056 at 3132.140 kb on + strand, within GFF3056 at 3132.140 kb on + strand, within GFF3056 at 3132.140 kb on + strand, within GFF3056 at 3132.145 kb on + strand, within GFF3056 at 3132.148 kb on - strand, within GFF3056 at 3132.148 kb on - strand, within GFF3056 at 3132.150 kb on + strand, within GFF3056 at 3132.207 kb on - strand at 3132.375 kb on - strand, within GFF3057 at 3132.398 kb on + strand, within GFF3057 at 3132.523 kb on - strand, within GFF3057 at 3132.556 kb on + strand, within GFF3057 at 3132.583 kb on - strand, within GFF3057 at 3132.603 kb on - strand, within GFF3057 at 3132.664 kb on + strand, within GFF3057 at 3132.696 kb on - strand, within GFF3057 at 3132.696 kb on - strand, within GFF3057 at 3132.732 kb on + strand at 3132.750 kb on + strand at 3132.774 kb on - strand at 3132.795 kb on + strand at 3132.799 kb on + strand at 3132.803 kb on - strand at 3132.843 kb on + strand at 3132.845 kb on + strand at 3132.847 kb on + strand at 3132.853 kb on - strand at 3132.861 kb on - strand at 3132.870 kb on + strand at 3132.881 kb on + strand at 3132.881 kb on + strand at 3132.934 kb on + strand, within GFF3058 at 3132.941 kb on - strand, within GFF3058 at 3132.941 kb on - strand, within GFF3058 at 3132.971 kb on + strand, within GFF3058 at 3132.972 kb on - strand, within GFF3058 at 3133.016 kb on + strand, within GFF3058 at 3133.024 kb on - strand, within GFF3058 at 3133.039 kb on + strand, within GFF3058 at 3133.049 kb on + strand, within GFF3058 at 3133.057 kb on - strand, within GFF3058 at 3133.057 kb on - strand, within GFF3058 at 3133.058 kb on - strand, within GFF3058 at 3133.059 kb on + strand, within GFF3058 at 3133.063 kb on + strand, within GFF3058 at 3133.064 kb on + strand, within GFF3058 at 3133.089 kb on + strand, within GFF3058 at 3133.092 kb on - strand, within GFF3058 at 3133.094 kb on + strand, within GFF3058 at 3133.094 kb on + strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.102 kb on - strand, within GFF3058 at 3133.128 kb on - strand, within GFF3058 at 3133.169 kb on - strand, within GFF3058 at 3133.172 kb on + strand, within GFF3058 at 3133.195 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.196 kb on + strand, within GFF3058 at 3133.201 kb on + strand, within GFF3058 at 3133.204 kb on - strand, within GFF3058 at 3133.217 kb on + strand, within GFF3058 at 3133.217 kb on + strand, within GFF3058 at 3133.223 kb on - strand, within GFF3058 at 3133.227 kb on - strand, within GFF3058 at 3133.279 kb on + strand, within GFF3058 at 3133.279 kb on + strand, within GFF3058 at 3133.283 kb on + strand, within GFF3058 at 3133.284 kb on + strand, within GFF3058 at 3133.287 kb on - strand, within GFF3058 at 3133.291 kb on - strand, within GFF3058 at 3133.317 kb on - strand, within GFF3058 at 3133.319 kb on + strand, within GFF3058 at 3133.319 kb on + strand, within GFF3058 at 3133.322 kb on - strand, within GFF3058 at 3133.365 kb on + strand, within GFF3058 at 3133.365 kb on + strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.373 kb on - strand, within GFF3058 at 3133.382 kb on + strand, within GFF3058 at 3133.390 kb on - strand at 3133.390 kb on - strand at 3133.392 kb on + strand at 3133.397 kb on - strand at 3133.400 kb on - strand at 3133.400 kb on - strand at 3133.413 kb on + strand at 3133.414 kb on - strand at 3133.430 kb on + strand at 3133.437 kb on + strand at 3133.439 kb on - strand at 3133.450 kb on - strand at 3133.450 kb on - strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.494 kb on + strand at 3133.499 kb on + strand at 3133.502 kb on - strand at 3133.502 kb on - strand at 3133.502 kb on - strand at 3133.548 kb on - strand, within GFF3059 at 3133.550 kb on + strand, within GFF3059 at 3133.550 kb on + strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.558 kb on - strand, within GFF3059 at 3133.575 kb on - strand, within GFF3059 at 3133.647 kb on - strand, within GFF3059 at 3133.667 kb on - strand, within GFF3059 at 3133.667 kb on - strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.697 kb on + strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.705 kb on - strand, within GFF3059 at 3133.715 kb on - strand, within GFF3059 at 3133.715 kb on - strand, within GFF3059 at 3133.741 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.758 kb on + strand at 3133.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction EMM_noCarbon with Sucrose and Putrescine remove 3,131,334 - -0.5 3,131,393 - -1.2 3,131,469 + +0.9 3,131,481 + +0.4 3,131,530 + -2.5 3,131,557 - +0.3 3,131,587 - +2.6 3,131,593 - -1.6 3,131,593 - +0.7 3,131,593 - -0.3 3,131,598 + +0.7 3,131,623 - +0.1 3,131,628 - +0.6 3,131,628 - -0.2 3,131,682 + GFF3056 0.16 -0.7 3,131,718 + GFF3056 0.22 -0.7 3,131,718 + GFF3056 0.22 -0.2 3,131,718 + GFF3056 0.22 +0.5 3,131,801 + GFF3056 0.34 +1.7 3,131,803 + GFF3056 0.34 -2.3 3,131,805 + GFF3056 0.34 +0.9 3,131,818 + GFF3056 0.36 -2.7 3,131,818 + GFF3056 0.36 +0.3 3,131,826 - GFF3056 0.37 +0.1 3,131,901 + GFF3056 0.48 +0.1 3,131,901 + GFF3056 0.48 -1.5 3,131,911 + GFF3056 0.50 +0.1 3,131,944 + GFF3056 0.54 -1.9 3,131,951 + GFF3056 0.55 +1.5 3,131,956 + GFF3056 0.56 +1.0 3,131,956 + GFF3056 0.56 +0.2 3,131,977 - GFF3056 0.59 +0.1 3,132,005 - GFF3056 0.63 +0.8 3,132,007 - GFF3056 0.63 -0.8 3,132,046 - GFF3056 0.69 -0.6 3,132,068 + GFF3056 0.72 -0.5 3,132,068 + GFF3056 0.72 +1.2 3,132,068 + GFF3056 0.72 -0.3 3,132,068 + GFF3056 0.72 -0.0 3,132,068 + GFF3056 0.72 -0.2 3,132,068 + GFF3056 0.72 +0.3 3,132,076 - GFF3056 0.73 -1.7 3,132,076 - GFF3056 0.73 -2.0 3,132,076 - GFF3056 0.73 +0.2 3,132,076 - GFF3056 0.73 -0.3 3,132,076 - GFF3056 0.73 -0.7 3,132,076 - GFF3056 0.73 -0.1 3,132,133 + GFF3056 0.82 +0.3 3,132,140 + GFF3056 0.83 +0.7 3,132,140 + GFF3056 0.83 -0.5 3,132,140 + GFF3056 0.83 +0.7 3,132,140 + GFF3056 0.83 -0.0 3,132,145 + GFF3056 0.83 -0.4 3,132,148 - GFF3056 0.84 -0.7 3,132,148 - GFF3056 0.84 +0.6 3,132,150 + GFF3056 0.84 +0.8 3,132,207 - -1.7 3,132,375 - GFF3057 0.19 +0.9 3,132,398 + GFF3057 0.23 -0.5 3,132,523 - GFF3057 0.50 -0.4 3,132,556 + GFF3057 0.57 -0.0 3,132,583 - GFF3057 0.62 +0.4 3,132,603 - GFF3057 0.66 -1.0 3,132,664 + GFF3057 0.79 -0.9 3,132,696 - GFF3057 0.86 +0.2 3,132,696 - GFF3057 0.86 -0.2 3,132,732 + -0.5 3,132,750 + -0.4 3,132,774 - -0.3 3,132,795 + +1.3 3,132,799 + -0.3 3,132,803 - -0.1 3,132,843 + -0.2 3,132,845 + -2.0 3,132,847 + +0.9 3,132,853 - -1.5 3,132,861 - -1.5 3,132,870 + -3.1 3,132,881 + -2.0 3,132,881 + -1.0 3,132,934 + GFF3058 0.12 -1.0 3,132,941 - GFF3058 0.14 -1.8 3,132,941 - GFF3058 0.14 -2.4 3,132,971 + GFF3058 0.19 -1.7 3,132,972 - GFF3058 0.19 -1.5 3,133,016 + GFF3058 0.26 -2.8 3,133,024 - GFF3058 0.28 -1.7 3,133,039 + GFF3058 0.30 -2.9 3,133,049 + GFF3058 0.32 -2.8 3,133,057 - GFF3058 0.33 -1.5 3,133,057 - GFF3058 0.33 -1.8 3,133,058 - GFF3058 0.34 -1.7 3,133,059 + GFF3058 0.34 -1.2 3,133,063 + GFF3058 0.34 -2.2 3,133,064 + GFF3058 0.35 -2.6 3,133,089 + GFF3058 0.39 -2.0 3,133,092 - GFF3058 0.39 -0.6 3,133,094 + GFF3058 0.40 -1.5 3,133,094 + GFF3058 0.40 -1.6 3,133,102 - GFF3058 0.41 -3.1 3,133,102 - GFF3058 0.41 -1.9 3,133,102 - GFF3058 0.41 -3.4 3,133,102 - GFF3058 0.41 -1.9 3,133,128 - GFF3058 0.45 -2.5 3,133,169 - GFF3058 0.52 -3.3 3,133,172 + GFF3058 0.53 -0.7 3,133,195 + GFF3058 0.57 -1.6 3,133,196 + GFF3058 0.57 -2.7 3,133,196 + GFF3058 0.57 -2.0 3,133,196 + GFF3058 0.57 -2.9 3,133,201 + GFF3058 0.58 -1.6 3,133,204 - GFF3058 0.58 -1.2 3,133,217 + GFF3058 0.61 +0.1 3,133,217 + GFF3058 0.61 -3.5 3,133,223 - GFF3058 0.62 -1.3 3,133,227 - GFF3058 0.62 -1.0 3,133,279 + GFF3058 0.71 -1.9 3,133,279 + GFF3058 0.71 -1.8 3,133,283 + GFF3058 0.72 -3.1 3,133,284 + GFF3058 0.72 -2.8 3,133,287 - GFF3058 0.72 -0.6 3,133,291 - GFF3058 0.73 -2.0 3,133,317 - GFF3058 0.78 -2.8 3,133,319 + GFF3058 0.78 -2.2 3,133,319 + GFF3058 0.78 -1.2 3,133,322 - GFF3058 0.78 -1.2 3,133,365 + GFF3058 0.86 -2.1 3,133,365 + GFF3058 0.86 -2.0 3,133,373 - GFF3058 0.87 -1.1 3,133,373 - GFF3058 0.87 -2.2 3,133,373 - GFF3058 0.87 -0.0 3,133,373 - GFF3058 0.87 -2.3 3,133,373 - GFF3058 0.87 -0.2 3,133,382 + GFF3058 0.89 -1.9 3,133,390 - -3.2 3,133,390 - -1.3 3,133,392 + -2.7 3,133,397 - +1.0 3,133,400 - -1.8 3,133,400 - -2.8 3,133,413 + -1.3 3,133,414 - -0.0 3,133,430 + +0.1 3,133,437 + -1.1 3,133,439 - -2.5 3,133,450 - -0.2 3,133,450 - +0.7 3,133,494 + -1.1 3,133,494 + +0.9 3,133,494 + +0.2 3,133,494 + +0.9 3,133,494 + +0.1 3,133,499 + -0.9 3,133,502 - -0.7 3,133,502 - -1.2 3,133,502 - -0.9 3,133,548 - GFF3059 0.25 -1.6 3,133,550 + GFF3059 0.25 -0.9 3,133,550 + GFF3059 0.25 -2.9 3,133,558 - GFF3059 0.28 -2.8 3,133,558 - GFF3059 0.28 -2.0 3,133,558 - GFF3059 0.28 -2.6 3,133,558 - GFF3059 0.28 -2.4 3,133,575 - GFF3059 0.34 -1.3 3,133,647 - GFF3059 0.59 -0.8 3,133,667 - GFF3059 0.66 -1.6 3,133,667 - GFF3059 0.66 -2.5 3,133,697 + GFF3059 0.76 -0.5 3,133,697 + GFF3059 0.76 -0.0 3,133,697 + GFF3059 0.76 -0.2 3,133,697 + GFF3059 0.76 -0.2 3,133,697 + GFF3059 0.76 -0.9 3,133,697 + GFF3059 0.76 +1.6 3,133,705 - GFF3059 0.79 -0.5 3,133,705 - GFF3059 0.79 -2.7 3,133,705 - GFF3059 0.79 -0.8 3,133,715 - GFF3059 0.83 -1.7 3,133,715 - GFF3059 0.83 -2.5 3,133,741 + +0.3 3,133,758 + +0.3 3,133,758 + -0.2 3,133,758 + -1.6 3,133,758 + -0.7 3,133,761 - -3.0
Or see this region's nucleotide sequence